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The map label for this gene is NemA [H]

Identifier: 20806554

GI number: 20806554

Start: 14153

End: 15166

Strand: Reverse

Name: NemA [H]

Synonym: TTE0012

Alternate gene names: 20806554

Gene position: 15166-14153 (Counterclockwise)

Preceding gene: 20806555

Following gene: 20806551

Centisome position: 0.56

GC content: 35.9

Gene sequence:

>1014_bases
ATGAGTATTTTACATACACCTTTAAAGATAAAGGATATTACAATAAAAAACAGAATCATGATGTCTCCCATGTGTATGTA
TACAGCTTCTACTGAGGGAATGCCAAATGACTGGCATATAGTTCATTATGCCACAAGGGCTATTGGTGGAGTAGGACTCA
TTATGCAAGAAGCTACAGCTGTTGAAAGCAGAGGAAGAATAACTGACCGTGACCTTGGCATATGGAATGATGAGCAAGCT
GAAGAATTAAAAAAAATTGTAGACATCTGCAAAGCAAATGGCGCTGTAATGGGAATACAGCTTGCTCATGCAGGAAGAAA
ATGTAATATACCCTATGAAGATGTTGTAGGACCTTCCCCTATTAAAGCAGGAGACCAGTACAAACTCCCAAGAGAATTAA
CCATTGATGAAATAAAATCTATAGTAAAAGCTTTTGGAGAAGCTGCCAAAAGGGCCAACTTAGCAGGTTATGATGTGGTG
GAAATACATGCAGCTCACGGCTATTTAATTCATGAATTTCTTTCTCCTCTTTCAAATAAACGAAAAGATGAATACGGCAA
TAGTATTGAAAATAGAGCAAGATTTTTAATTGAAGTGATAGATGAAGTTAGAAAAAATTGGCCTCAAAACAAACCTATTT
TCGTGCGCGTATCTGCAGACGATTACATTGAAGGCGGAATAAACATAGATATGATGGTAGAATATATCAATATGATAAAA
GACAAAGTTGATGTAATTGATGTAAGCAGTGGGGGACTTTTAAATGTTGATATAAACCCATATCCCGGATATCAAGTTAA
ATATGCTGAAACAATTAAAAAGCGCTGCAATATAAAAACCTGTGCAGTAGGATTAATAACGACACAAGAACTTGCAGAAG
AAATTCTTTCAAATGAAAGGGCTGACTTGGTTGCACTGGGAAGAGAACTTTTAAGAAATCCCTATTGGGTTTTGCATACA
TACACTTCAAAGGAAGACTGGCCAAAACAATATGAAAGAGCATTTAAAAACTAA

Upstream 100 bases:

>100_bases
AATGGTAATATAAGCCATGAGATGCTCTTGTTGTGATGAGAAAATTAAAGAAAAACTAAGATATTAAGAGAAAATAAAAG
ATAATAGAGGGAGGGTGTTT

Downstream 100 bases:

>100_bases
GCCCCCAAAACTTGAGGGCTTAGTTTTATTCCTCACTTTCAATTCTTGCCGTAGAGACCACTCTATCTCCGTCTTCTAAT
TTCATAAGGGTGACTCCTTG

Product: NADH:flavin oxidoreductase

Products: NA

Alternate protein names: Xenobiotic reductase [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSILHTPLKIKDITIKNRIMMSPMCMYTASTEGMPNDWHIVHYATRAIGGVGLIMQEATAVESRGRITDRDLGIWNDEQA
EELKKIVDICKANGAVMGIQLAHAGRKCNIPYEDVVGPSPIKAGDQYKLPRELTIDEIKSIVKAFGEAAKRANLAGYDVV
EIHAAHGYLIHEFLSPLSNKRKDEYGNSIENRARFLIEVIDEVRKNWPQNKPIFVRVSADDYIEGGINIDMMVEYINMIK
DKVDVIDVSSGGLLNVDINPYPGYQVKYAETIKKRCNIKTCAVGLITTQELAEEILSNERADLVALGRELLRNPYWVLHT
YTSKEDWPKQYERAFKN

Sequences:

>Translated_337_residues
MSILHTPLKIKDITIKNRIMMSPMCMYTASTEGMPNDWHIVHYATRAIGGVGLIMQEATAVESRGRITDRDLGIWNDEQA
EELKKIVDICKANGAVMGIQLAHAGRKCNIPYEDVVGPSPIKAGDQYKLPRELTIDEIKSIVKAFGEAAKRANLAGYDVV
EIHAAHGYLIHEFLSPLSNKRKDEYGNSIENRARFLIEVIDEVRKNWPQNKPIFVRVSADDYIEGGINIDMMVEYINMIK
DKVDVIDVSSGGLLNVDINPYPGYQVKYAETIKKRCNIKTCAVGLITTQELAEEILSNERADLVALGRELLRNPYWVLHT
YTSKEDWPKQYERAFKN
>Mature_336_residues
SILHTPLKIKDITIKNRIMMSPMCMYTASTEGMPNDWHIVHYATRAIGGVGLIMQEATAVESRGRITDRDLGIWNDEQAE
ELKKIVDICKANGAVMGIQLAHAGRKCNIPYEDVVGPSPIKAGDQYKLPRELTIDEIKSIVKAFGEAAKRANLAGYDVVE
IHAAHGYLIHEFLSPLSNKRKDEYGNSIENRARFLIEVIDEVRKNWPQNKPIFVRVSADDYIEGGINIDMMVEYINMIKD
KVDVIDVSSGGLLNVDINPYPGYQVKYAETIKKRCNIKTCAVGLITTQELAEEILSNERADLVALGRELLRNPYWVLHTY
TSKEDWPKQYERAFKN

Specific function: Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=356, Percent_Identity=31.7415730337079, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789463, Length=330, Percent_Identity=27.8787878787879, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17540738, Length=340, Percent_Identity=27.3529411764706, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17566914, Length=272, Percent_Identity=30.8823529411765, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17559804, Length=237, Percent_Identity=30.8016877637131, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17559802, Length=335, Percent_Identity=25.9701492537313, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17565138, Length=257, Percent_Identity=28.0155642023346, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI72001454, Length=309, Percent_Identity=25.8899676375405, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17564188, Length=309, Percent_Identity=24.9190938511327, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325086, Length=216, Percent_Identity=37.037037037037, Blast_Score=122, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6321973, Length=216, Percent_Identity=37.037037037037, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: =1.6.99.1 [H]

Molecular weight: Translated: 38112; Mature: 37981

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILHTPLKIKDITIKNRIMMSPMCMYTASTEGMPNDWHIVHYATRAIGGVGLIMQEATA
CCCCCCCCEEEEEEECCHHEECCCEEEEECCCCCCCCEEEEEEHHHHHCHHHHHHHHHHH
VESRGRITDRDLGIWNDEQAEELKKIVDICKANGAVMGIQLAHAGRKCNIPYEDVVGPSP
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCCCCC
IKAGDQYKLPRELTIDEIKSIVKAFGEAAKRANLAGYDVVEIHAAHGYLIHEFLSPLSNK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHC
RKDEYGNSIENRARFLIEVIDEVRKNWPQNKPIFVRVSADDYIEGGINIDMMVEYINMIK
CHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHCCCCCHHHHHHHHHHHH
DKVDVIDVSSGGLLNVDINPYPGYQVKYAETIKKRCNIKTCAVGLITTQELAEEILSNER
HHCCEEEECCCCEEEEECCCCCCCEEEHHHHHHHHCCCCHHHEEHHHHHHHHHHHHCCCC
ADLVALGRELLRNPYWVLHTYTSKEDWPKQYERAFKN
HHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SILHTPLKIKDITIKNRIMMSPMCMYTASTEGMPNDWHIVHYATRAIGGVGLIMQEATA
CCCCCCCEEEEEEECCHHEECCCEEEEECCCCCCCCEEEEEEHHHHHCHHHHHHHHHHH
VESRGRITDRDLGIWNDEQAEELKKIVDICKANGAVMGIQLAHAGRKCNIPYEDVVGPSP
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCCCCC
IKAGDQYKLPRELTIDEIKSIVKAFGEAAKRANLAGYDVVEIHAAHGYLIHEFLSPLSNK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHC
RKDEYGNSIENRARFLIEVIDEVRKNWPQNKPIFVRVSADDYIEGGINIDMMVEYINMIK
CHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHCCCCCHHHHHHHHHHHH
DKVDVIDVSSGGLLNVDINPYPGYQVKYAETIKKRCNIKTCAVGLITTQELAEEILSNER
HHCCEEEECCCCEEEEECCCCCCCEEEHHHHHHHHCCCCHHHEEHHHHHHHHHHHHCCCC
ADLVALGRELLRNPYWVLHTYTSKEDWPKQYERAFKN
HHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]