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The map label for this gene is recF

Identifier: 20806546

GI number: 20806546

Start: 3277

End: 4371

Strand: Direct

Name: recF

Synonym: TTE0004

Alternate gene names: 20806546

Gene position: 3277-4371 (Clockwise)

Preceding gene: 20806545

Following gene: 20806547

Centisome position: 0.12

GC content: 33.88

Gene sequence:

>1095_bases
TTGTACTTAAAGGAAATTTTTGTAGACAATTTTAGAAATTTAAAAAAGCAAAAATTAGAATTCTGTGAAGGAGTCAATTT
GATATATGGGCTAAATGCACAAGGGAAGAGCAATCTTTTAGAGGCAATAAGGCTTCTCAGCATGGGAAGGTCCTTCAGAG
GAAGTAAAATGTCTGAGCTTGTGAAATTTGATGAAGAGTATTTTTACGTGAGAGGTCTAGTCAGAAGTGCGGATTTTTAT
GAAAAGAAAATAGAATTTGGGTATAAGGTAAATGGAAATAAGGTTATTAAAGTAAATGGCAATAAGTTAAAGAGCACAGG
AGAGATTTTGGGGCACTTTTTGACAGTTATATTTTCCCCTGAGGACATAGAGATAATAAAAGAAGGGCCTTCCCGCAGAA
GAAAATATTTAGATGCTTGCATTTCCGTCATTGACAAAAATTATTTTTTTGACCTTCTTCAGTACAATAAAACCCTATCT
AACAGGAATAGCTTGCTTAAAAAGATAAAAGAAGAAGGAAAAGGAGAAGATTTATTGGAAATATTTGATGAAAAGCTGGC
AGAGTATGGAGCTAGAATAATAAAGGTGAGAAATAATTACCTTGAGAAATTAAAAAATAGCATGTCAAAATTTTTAATGG
AAATTTCTAATGAAAAGTTGGAAATCATATATTTAAACAGCGCAGGAGTAAAGGAAGTGCACGAAGAAAATCTCATAAGA
GAAAAGTTAAAAAATAGGCTGACTAAAAGTTTGACCCTAGATTTAAAATATCTGTCTACACAAGTGGGTCCTCATAGGGA
AGATTTTAAGATATTGATAAATGGATATGATTCTAGGGTGTATTCGTCACAAGGGCAGAAAAGGACAGCTGCCCTGTGCC
TCAAGCTTTCAGAACTAGAGATTTTAGAAGAAGAGACTGGAGAAAAGCCTGTTTTGCTTTTGGATGACGTGATGTCGGAG
TTGGACGATAACAGAAAGAAATACATTTTAAAAAAGCTTGAAGGCTTTCAGTCTTTTATCACCCACACTTCAAAGTCAGA
TGTAGAGGGAGACTGTTGTTTTAAAATTTATGATGGAATTGTCATGAGAGAATAG

Upstream 100 bases:

>100_bases
GTAAACGGAGTTATAGAGCTTAAAAGAGGAAGGAAACTTTATAAAAATGATATAATTGAAGTGGAAGGAAAGACTTTTGT
GATAAAGTGAGGAGTCCATC

Downstream 100 bases:

>100_bases
GGGGTTTTTTAAATGTACATTCATTTAGGTGGAGACGTAGTAGTACCGGACAAAGAAATCATCGGAATTTTTGACATGAA
TATTGTCTCTACTTCCTCCA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MYLKEIFVDNFRNLKKQKLEFCEGVNLIYGLNAQGKSNLLEAIRLLSMGRSFRGSKMSELVKFDEEYFYVRGLVRSADFY
EKKIEFGYKVNGNKVIKVNGNKLKSTGEILGHFLTVIFSPEDIEIIKEGPSRRRKYLDACISVIDKNYFFDLLQYNKTLS
NRNSLLKKIKEEGKGEDLLEIFDEKLAEYGARIIKVRNNYLEKLKNSMSKFLMEISNEKLEIIYLNSAGVKEVHEENLIR
EKLKNRLTKSLTLDLKYLSTQVGPHREDFKILINGYDSRVYSSQGQKRTAALCLKLSELEILEEETGEKPVLLLDDVMSE
LDDNRKKYILKKLEGFQSFITHTSKSDVEGDCCFKIYDGIVMRE

Sequences:

>Translated_364_residues
MYLKEIFVDNFRNLKKQKLEFCEGVNLIYGLNAQGKSNLLEAIRLLSMGRSFRGSKMSELVKFDEEYFYVRGLVRSADFY
EKKIEFGYKVNGNKVIKVNGNKLKSTGEILGHFLTVIFSPEDIEIIKEGPSRRRKYLDACISVIDKNYFFDLLQYNKTLS
NRNSLLKKIKEEGKGEDLLEIFDEKLAEYGARIIKVRNNYLEKLKNSMSKFLMEISNEKLEIIYLNSAGVKEVHEENLIR
EKLKNRLTKSLTLDLKYLSTQVGPHREDFKILINGYDSRVYSSQGQKRTAALCLKLSELEILEEETGEKPVLLLDDVMSE
LDDNRKKYILKKLEGFQSFITHTSKSDVEGDCCFKIYDGIVMRE
>Mature_364_residues
MYLKEIFVDNFRNLKKQKLEFCEGVNLIYGLNAQGKSNLLEAIRLLSMGRSFRGSKMSELVKFDEEYFYVRGLVRSADFY
EKKIEFGYKVNGNKVIKVNGNKLKSTGEILGHFLTVIFSPEDIEIIKEGPSRRRKYLDACISVIDKNYFFDLLQYNKTLS
NRNSLLKKIKEEGKGEDLLEIFDEKLAEYGARIIKVRNNYLEKLKNSMSKFLMEISNEKLEIIYLNSAGVKEVHEENLIR
EKLKNRLTKSLTLDLKYLSTQVGPHREDFKILINGYDSRVYSSQGQKRTAALCLKLSELEILEEETGEKPVLLLDDVMSE
LDDNRKKYILKKLEGFQSFITHTSKSDVEGDCCFKIYDGIVMRE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=356, Percent_Identity=26.123595505618, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_THETN (Q8RDL3)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_621717.1
- ProteinModelPortal:   Q8RDL3
- SMR:   Q8RDL3
- GeneID:   996566
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0004
- NMPDR:   fig|273068.3.peg.2
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   CLSK900569
- BioCyc:   TTEN273068:TTE0004-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42276; Mature: 42276

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLKEIFVDNFRNLKKQKLEFCEGVNLIYGLNAQGKSNLLEAIRLLSMGRSFRGSKMSEL
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
VKFDEEYFYVRGLVRSADFYEKKIEFGYKVNGNKVIKVNGNKLKSTGEILGHFLTVIFSP
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCC
EDIEIIKEGPSRRRKYLDACISVIDKNYFFDLLQYNKTLSNRNSLLKKIKEEGKGEDLLE
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
IFDEKLAEYGARIIKVRNNYLEKLKNSMSKFLMEISNEKLEIIYLNSAGVKEVHEENLIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
EKLKNRLTKSLTLDLKYLSTQVGPHREDFKILINGYDSRVYSSQGQKRTAALCLKLSELE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHH
ILEEETGEKPVLLLDDVMSELDDNRKKYILKKLEGFQSFITHTSKSDVEGDCCFKIYDGI
HHHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCC
VMRE
CCCC
>Mature Secondary Structure
MYLKEIFVDNFRNLKKQKLEFCEGVNLIYGLNAQGKSNLLEAIRLLSMGRSFRGSKMSEL
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHH
VKFDEEYFYVRGLVRSADFYEKKIEFGYKVNGNKVIKVNGNKLKSTGEILGHFLTVIFSP
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCC
EDIEIIKEGPSRRRKYLDACISVIDKNYFFDLLQYNKTLSNRNSLLKKIKEEGKGEDLLE
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
IFDEKLAEYGARIIKVRNNYLEKLKNSMSKFLMEISNEKLEIIYLNSAGVKEVHEENLIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHH
EKLKNRLTKSLTLDLKYLSTQVGPHREDFKILINGYDSRVYSSQGQKRTAALCLKLSELE
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHHHH
ILEEETGEKPVLLLDDVMSELDDNRKKYILKKLEGFQSFITHTSKSDVEGDCCFKIYDGI
HHHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCC
VMRE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336