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The map label for this gene is DnaN [H]

Identifier: 20806544

GI number: 20806544

Start: 1934

End: 3046

Strand: Direct

Name: DnaN [H]

Synonym: TTE0002

Alternate gene names: 20806544

Gene position: 1934-3046 (Clockwise)

Preceding gene: 20806543

Following gene: 20806545

Centisome position: 0.07

GC content: 37.74

Gene sequence:

>1113_bases
ATGAAATTTGTGTGTGATAAAAATTCATTGTTGGAAGGCGTCAATATAGCCATAAGGGGGGTATCCTCCCGTACCACCCT
TCCCATATTGCAAGGAATAAAAATAACAGCAAGAGGCAATGTCATAAAGCTTTCAGGTACTGACCTCGAGATAGGGATAG
AGTGTCAAATACCCGCAGTTATTGAAGAAGAGGGGGAGACAGTTGTTCCAGCAAGGATTTTTAGTGACCTCGTAAAAAAA
TTGCCTGAAGGAGAAGTGGAAGTAAAAAGCGATTCACAGAATACTGTAAATGTGGTTTCAGGAGACATAAACTTCTCAAT
TGCAGGAAGCAATCCAGAAGAATTTCCTGAAATACCTGAAGTATCAAGAGAAAAGTCATTTAAACTTCCCCAATCAATCC
TCAAAGACTTGATAAAAAAGACAGTTTTTTGCGTCTCAGAAGAGCAGACTAGGCCAATTCTAACAGGGGTACTTTTTGAA
GTATTTCCAAATGAGCTTAAAGCAGTGGCATTGGACGGATTTAGAATGGCCATATACTCTTATAAGTCGGAAAAGTCCTT
TTTTGACGAAGAAGCGGAGAAGTACTCTCTTGTCATTCCGGGAGATACCATCGATGAAATTTCAAGGATATTGGAAGATG
AAGAGACAGAGGTAATAATATACCACACTTCCAACCAGGTGCTTTTCCAGATTGATAACACTAAAGTCATCTCAAGGCTT
CTTGAAGGGAGTTTTATAAACTACAACGCTGTGCTCCCTAAAGATTTTAAGACAGAGATCACTATAAATAAAGATGTGTT
TATGGAAAGCCTTGAAAGGGCATCTCTAATTGCTGAGAGCAAGAACAATTTAGTAAAATTTGAAATAGGAGATAGCTTTA
TTGTGATTTCTTCAAGTTCGGAAAAAGGAAGTATGTCAGAAAAGTTGGAAGTGGAAGTTAAAGGAATGCTTCTAGAGATT
GCTTTTAACTCTAGATATTTACTTGATGCGCTCAAGGCAATTAATGAAGAAGAAGTAAATCTTTACTTCATAAACAGCAT
AAATCCGCTAATAATAAAACCAGTGGGGGAAAAGGAATACCTCTACATGATACTGCCGGTGAAGCTTAACTAA

Upstream 100 bases:

>100_bases
AAATTCACAATCTCTACTCTTACTACTACTAGATTTTGTTGTACAAAAATATTATATGCTAGGCCTTCAAAAAATATTCT
CAAAAACAGGAGTGAAAAAT

Downstream 100 bases:

>100_bases
GAGGTGTTATTTGATGATAGAGATTCCCATCAAAACAGAGTATATAACTTTGGGACAGTTTTTGAAGTATGCAAAGGTGT
GCGATACAGGTGGACAAGCA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKFVCDKNSLLEGVNIAIRGVSSRTTLPILQGIKITARGNVIKLSGTDLEIGIECQIPAVIEEEGETVVPARIFSDLVKK
LPEGEVEVKSDSQNTVNVVSGDINFSIAGSNPEEFPEIPEVSREKSFKLPQSILKDLIKKTVFCVSEEQTRPILTGVLFE
VFPNELKAVALDGFRMAIYSYKSEKSFFDEEAEKYSLVIPGDTIDEISRILEDEETEVIIYHTSNQVLFQIDNTKVISRL
LEGSFINYNAVLPKDFKTEITINKDVFMESLERASLIAESKNNLVKFEIGDSFIVISSSSEKGSMSEKLEVEVKGMLLEI
AFNSRYLLDALKAINEEEVNLYFINSINPLIIKPVGEKEYLYMILPVKLN

Sequences:

>Translated_370_residues
MKFVCDKNSLLEGVNIAIRGVSSRTTLPILQGIKITARGNVIKLSGTDLEIGIECQIPAVIEEEGETVVPARIFSDLVKK
LPEGEVEVKSDSQNTVNVVSGDINFSIAGSNPEEFPEIPEVSREKSFKLPQSILKDLIKKTVFCVSEEQTRPILTGVLFE
VFPNELKAVALDGFRMAIYSYKSEKSFFDEEAEKYSLVIPGDTIDEISRILEDEETEVIIYHTSNQVLFQIDNTKVISRL
LEGSFINYNAVLPKDFKTEITINKDVFMESLERASLIAESKNNLVKFEIGDSFIVISSSSEKGSMSEKLEVEVKGMLLEI
AFNSRYLLDALKAINEEEVNLYFINSINPLIIKPVGEKEYLYMILPVKLN
>Mature_370_residues
MKFVCDKNSLLEGVNIAIRGVSSRTTLPILQGIKITARGNVIKLSGTDLEIGIECQIPAVIEEEGETVVPARIFSDLVKK
LPEGEVEVKSDSQNTVNVVSGDINFSIAGSNPEEFPEIPEVSREKSFKLPQSILKDLIKKTVFCVSEEQTRPILTGVLFE
VFPNELKAVALDGFRMAIYSYKSEKSFFDEEAEKYSLVIPGDTIDEISRILEDEETEVIIYHTSNQVLFQIDNTKVISRL
LEGSFINYNAVLPKDFKTEITINKDVFMESLERASLIAESKNNLVKFEIGDSFIVISSSSEKGSMSEKLEVEVKGMLLEI
AFNSRYLLDALKAINEEEVNLYFINSINPLIIKPVGEKEYLYMILPVKLN

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=372, Percent_Identity=29.3010752688172, Blast_Score=175, Evalue=4e-45,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41454; Mature: 41454

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFVCDKNSLLEGVNIAIRGVSSRTTLPILQGIKITARGNVIKLSGTDLEIGIECQIPAV
CCEEECCCHHHCCCEEEEEECCCCCCCHHHCCEEEEECCCEEEEECCCEEEEEEEECCCE
IEEEGETVVPARIFSDLVKKLPEGEVEVKSDSQNTVNVVSGDINFSIAGSNPEEFPEIPE
EECCCCEEEHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCEEEEEECCCHHHCCCCCC
VSREKSFKLPQSILKDLIKKTVFCVSEEQTRPILTGVLFEVFPNELKAVALDGFRMAIYS
CCCCCCCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCEEEEEEE
YKSEKSFFDEEAEKYSLVIPGDTIDEISRILEDEETEVIIYHTSNQVLFQIDNTKVISRL
ECCCHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEECCCEEEEEECCHHHHHHH
LEGSFINYNAVLPKDFKTEITINKDVFMESLERASLIAESKNNLVKFEIGDSFIVISSSS
HCCCEEEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCC
EKGSMSEKLEVEVKGMLLEIAFNSRYLLDALKAINEEEVNLYFINSINPLIIKPVGEKEY
CCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCE
LYMILPVKLN
EEEEEEEEEC
>Mature Secondary Structure
MKFVCDKNSLLEGVNIAIRGVSSRTTLPILQGIKITARGNVIKLSGTDLEIGIECQIPAV
CCEEECCCHHHCCCEEEEEECCCCCCCHHHCCEEEEECCCEEEEECCCEEEEEEEECCCE
IEEEGETVVPARIFSDLVKKLPEGEVEVKSDSQNTVNVVSGDINFSIAGSNPEEFPEIPE
EECCCCEEEHHHHHHHHHHHCCCCCEEEECCCCCEEEEEECCEEEEEECCCHHHCCCCCC
VSREKSFKLPQSILKDLIKKTVFCVSEEQTRPILTGVLFEVFPNELKAVALDGFRMAIYS
CCCCCCCCCHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCEEEEEEE
YKSEKSFFDEEAEKYSLVIPGDTIDEISRILEDEETEVIIYHTSNQVLFQIDNTKVISRL
ECCCHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEECCCEEEEEECCHHHHHHH
LEGSFINYNAVLPKDFKTEITINKDVFMESLERASLIAESKNNLVKFEIGDSFIVISSSS
HCCCEEEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEECCC
EKGSMSEKLEVEVKGMLLEIAFNSRYLLDALKAINEEEVNLYFINSINPLIIKPVGEKEY
CCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCE
LYMILPVKLN
EEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA