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The map label for this gene is dnaX [C]

Identifier: 203284658

GI number: 203284658

Start: 822306

End: 823349

Strand: Reverse

Name: dnaX [C]

Synonym: BDU_769

Alternate gene names: 203284658

Gene position: 823349-822306 (Counterclockwise)

Preceding gene: 203284659

Following gene: 203284657

Centisome position: 88.37

GC content: 21.55

Gene sequence:

>1044_bases
ATGAAAATATTACCTGAAATAGCTAAAACAATAATTAATGAATATAAAAAAGAAAATTTAGCAAATGCAATGCTTTTTTG
GGGAGAAAGATTTTCTTTTAAAAAGACAACTGCAATTGCACTAGCAAAAGAAATTCTAAATACAAAAAATTTAACAAATC
CTAATTTAATTATTCTCTCAGATCTTAATGTAGTGGAAGCAAAAGCTTATCTCAGCTCAACATCAAACGAAGTTATAAAT
CGATATCTAGAATATGTTAAAAATATTATCTTTGCAAAATACAACTTTAGTAGTGACAAAAATTTAAAAAAAATAGAAAC
AAATATCAATTTTATTAATGATGTCTACTATAACAATCAATACAATGACACAACAAAAAAAGAACTTATTAATAAAATTG
AAGAAATAATTAAAGAGATAAATTTTAACATTACAATTAAGGATGTTAGAAAAATTAAATACTGGGTACTATCTGAAAAA
AACAAGATAAAAGTAATTTACATTAATACAATTGAAAACCTTAATTTTAATGTCTATAATGCATTATTAAAAATACTAGA
AGAACCACCTTCAAATATCTATTTCATTTTAACCACAAGAAATAAAAATAAAATTCCAAAAACAATTTTATCAAGACTTA
GAATATACAAATTCAACAAAGAAAACAGAGAATTTGAAATTAGTCTATTTCAGGAAACTTTTAAAAAAAATGATAAACTT
ACAATAGAAGAATATTTTAAATCATTTAATAGCGAAGAACATACCAAATTAAAAGAAGAAGTCAAAAAAATTTTAAATGT
CATACAAGAAAAGCAAAGTATATTTAATCTAAGTTCAACTAATTTCATAAAAGACGACAACATATTCAGTCTATTTTTAG
AAGAACTTGCAAACCAACTTAGAAATGAATTTTTAAACCAAAATTTAGATATAAACACATACTTACAAAGATTAGAATAT
CTAAAATATATAATGAAATATAATCCTTACAATCAAAATAAAAAATTAATAATCGAAAATCTAATGTTACATTATGAGGA
TTAA

Upstream 100 bases:

>100_bases
CTAACTATTTCTTAGAAGGAAGTAACTTTCTTGAATATCTCAATCCTGGAATAAATTACCTATTTAAAATATCAAAAACA
TAGCAAAGAGAAGTAAAAAT

Downstream 100 bases:

>100_bases
TGAGCAAATTTTTAAAAAAAACATTGACCAAATTAAATAAATTATCAAGTGACCAAAAACTTAAATTCATACAAGATATT
TATAAAAAAATAGAAATATA

Product: DNA polymerase III gamma and tau subunit

Products: diphosphate; DNAn+1

Alternate protein names: DNA Polymerase III Gamma And Tau Subunit; DNA Polymerase III Gamma/Tau Subunits

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKILPEIAKTIINEYKKENLANAMLFWGERFSFKKTTAIALAKEILNTKNLTNPNLIILSDLNVVEAKAYLSSTSNEVIN
RYLEYVKNIIFAKYNFSSDKNLKKIETNINFINDVYYNNQYNDTTKKELINKIEEIIKEINFNITIKDVRKIKYWVLSEK
NKIKVIYINTIENLNFNVYNALLKILEEPPSNIYFILTTRNKNKIPKTILSRLRIYKFNKENREFEISLFQETFKKNDKL
TIEEYFKSFNSEEHTKLKEEVKKILNVIQEKQSIFNLSSTNFIKDDNIFSLFLEELANQLRNEFLNQNLDINTYLQRLEY
LKYIMKYNPYNQNKKLIIENLMLHYED

Sequences:

>Translated_347_residues
MKILPEIAKTIINEYKKENLANAMLFWGERFSFKKTTAIALAKEILNTKNLTNPNLIILSDLNVVEAKAYLSSTSNEVIN
RYLEYVKNIIFAKYNFSSDKNLKKIETNINFINDVYYNNQYNDTTKKELINKIEEIIKEINFNITIKDVRKIKYWVLSEK
NKIKVIYINTIENLNFNVYNALLKILEEPPSNIYFILTTRNKNKIPKTILSRLRIYKFNKENREFEISLFQETFKKNDKL
TIEEYFKSFNSEEHTKLKEEVKKILNVIQEKQSIFNLSSTNFIKDDNIFSLFLEELANQLRNEFLNQNLDINTYLQRLEY
LKYIMKYNPYNQNKKLIIENLMLHYED
>Mature_347_residues
MKILPEIAKTIINEYKKENLANAMLFWGERFSFKKTTAIALAKEILNTKNLTNPNLIILSDLNVVEAKAYLSSTSNEVIN
RYLEYVKNIIFAKYNFSSDKNLKKIETNINFINDVYYNNQYNDTTKKELINKIEEIIKEINFNITIKDVRKIKYWVLSEK
NKIKVIYINTIENLNFNVYNALLKILEEPPSNIYFILTTRNKNKIPKTILSRLRIYKFNKENREFEISLFQETFKKNDKL
TIEEYFKSFNSEEHTKLKEEVKKILNVIQEKQSIFNLSSTNFIKDDNIFSLFLEELANQLRNEFLNQNLDINTYLQRLEY
LKYIMKYNPYNQNKKLIIENLMLHYED

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Tau Chain Serves As A Scaffold To Help In The Dimerizaton Of The Core Co

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7

Molecular weight: Translated: 41565; Mature: 41565

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILPEIAKTIINEYKKENLANAMLFWGERFSFKKTTAIALAKEILNTKNLTNPNLIILS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
DLNVVEAKAYLSSTSNEVINRYLEYVKNIIFAKYNFSSDKNLKKIETNINFINDVYYNNQ
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHCCHHHHHHEECCC
YNDTTKKELINKIEEIIKEINFNITIKDVRKIKYWVLSEKNKIKVIYINTIENLNFNVYN
CCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHEECCCCCEEEEEEEEECCCCHHHHH
ALLKILEEPPSNIYFILTTRNKNKIPKTILSRLRIYKFNKENREFEISLFQETFKKNDKL
HHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHEECCCCCEEEHHHHHHHHCCCCCE
TIEEYFKSFNSEEHTKLKEEVKKILNVIQEKQSIFNLSSTNFIKDDNIFSLFLEELANQL
EHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
RNEFLNQNLDINTYLQRLEYLKYIMKYNPYNQNKKLIIENLMLHYED
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKILPEIAKTIINEYKKENLANAMLFWGERFSFKKTTAIALAKEILNTKNLTNPNLIILS
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
DLNVVEAKAYLSSTSNEVINRYLEYVKNIIFAKYNFSSDKNLKKIETNINFINDVYYNNQ
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHCCHHHHHHEECCC
YNDTTKKELINKIEEIIKEINFNITIKDVRKIKYWVLSEKNKIKVIYINTIENLNFNVYN
CCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHEECCCCCEEEEEEEEECCCCHHHHH
ALLKILEEPPSNIYFILTTRNKNKIPKTILSRLRIYKFNKENREFEISLFQETFKKNDKL
HHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHEECCCCCEEEHHHHHHHHCCCCCE
TIEEYFKSFNSEEHTKLKEEVKKILNVIQEKQSIFNLSSTNFIKDDNIFSLFLEELANQL
EHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
RNEFLNQNLDINTYLQRLEYLKYIMKYNPYNQNKKLIIENLMLHYED
HHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA