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The map label for this gene is kinE [H]

Identifier: 203284657

GI number: 203284657

Start: 821146

End: 822306

Strand: Reverse

Name: kinE [H]

Synonym: BDU_768

Alternate gene names: 203284657

Gene position: 822306-821146 (Counterclockwise)

Preceding gene: 203284658

Following gene: 203284656

Centisome position: 88.26

GC content: 22.65

Gene sequence:

>1161_bases
ATGAGCAAATTTTTAAAAAAAACATTGACCAAATTAAATAAATTATCAAGTGACCAAAAACTTAAATTCATACAAGATAT
TTATAAAAAAATAGAAATATATGATGGTATTTTCGCATCTATTAATGAAGGCATTATGGTACTTGATAAAATCAACAACA
TAATTTATCTAAACAAAATGTTATTTCAAATTCTATCTATAAGCCCCAAATATAAATTAGAAACTCTTAAAGATATTTCA
ATTCCAACACTAACAAATCTCATAGAAGAACTAGCAACAAATGAAGATAAAATAATAGGATTTGAAGTACAAATTTCAAC
AAACATATATATCAAAATATCATTTATGCCATATGTAAGAGATCAAAAACTTGAAGGAAATATCATTTTAATTGAAGACA
TTAAAGATAAAAAACATAAAGAAGAACTCTTTAGAAGAGCTGAAGCTTTAGCTGCATTTACAAGACATGCAAGAAACATA
GCACATGAAATTAAAAATCCTCTAGGAGCAATTGATATAAATTTACAACTCCTTAAAAAAGAAATAGATAAACAAAACAT
CGAAAGTACAAAAACAAATCATTACTTTAAAATAATTAAAGAAGAAATAAATAGAATGGACAAAACAGTAACAGATTTCT
TATTAACAGTAAGACCAATAAAAATAATGCCACAAAAAAAAGATATTTCAGATATTATAAAAAGTGTATATAATTTATTA
AATCCAGAATTAAAAAATAAAAATATTAAATTTATACTTAGTTTAAAAAAAGTAAGCTTTGTATTAATTGATGAAAAACT
TATAAGACAAGTAATTATTAATATAGTAAAAAATGCAGAAGAAGCATTACTTGAATCAAATAAAAAAATAAAAAAAATAG
AAATATCTATTCATGAAAATGAAAATAACATTTATGTTTCTATTAAAGATAATGCTAATGGAATAACAAACGAAACAAAA
GACGATATATTTAAACCACAATTTAGTACAAAAGAAAATGGAAGTGGTATAGGACTTACCATTTCCTATAAAATAGTGAA
AGAACATGGTGGTGAAATTTTTGTAGAAAGTAAAGAAATGAAAGGAACATCTTTTACAATAACACTTCCAAAATTAAATA
AAGGCAAAATTTTAATTGAAGGATGTTTAGAAAATGAGTAA

Upstream 100 bases:

>100_bases
TACAAAGATTAGAATATCTAAAATATATAATGAAATATAATCCTTACAATCAAAATAAAAAATTAATAATCGAAAATCTA
ATGTTACATTATGAGGATTA

Downstream 100 bases:

>100_bases
ATTATTGGTAGCAGATGATGAAAAAAACATACGAGAAGGAATAGCAACTTATCTTGAAGAAGAAGGTTACTTTGTATTTA
CTGCTAGTGATGGCGAAGAA

Product: sensory transduction histidine kinase, putative

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSKFLKKTLTKLNKLSSDQKLKFIQDIYKKIEIYDGIFASINEGIMVLDKINNIIYLNKMLFQILSISPKYKLETLKDIS
IPTLTNLIEELATNEDKIIGFEVQISTNIYIKISFMPYVRDQKLEGNIILIEDIKDKKHKEELFRRAEALAAFTRHARNI
AHEIKNPLGAIDINLQLLKKEIDKQNIESTKTNHYFKIIKEEINRMDKTVTDFLLTVRPIKIMPQKKDISDIIKSVYNLL
NPELKNKNIKFILSLKKVSFVLIDEKLIRQVIINIVKNAEEALLESNKKIKKIEISIHENENNIYVSIKDNANGITNETK
DDIFKPQFSTKENGSGIGLTISYKIVKEHGGEIFVESKEMKGTSFTITLPKLNKGKILIEGCLENE

Sequences:

>Translated_386_residues
MSKFLKKTLTKLNKLSSDQKLKFIQDIYKKIEIYDGIFASINEGIMVLDKINNIIYLNKMLFQILSISPKYKLETLKDIS
IPTLTNLIEELATNEDKIIGFEVQISTNIYIKISFMPYVRDQKLEGNIILIEDIKDKKHKEELFRRAEALAAFTRHARNI
AHEIKNPLGAIDINLQLLKKEIDKQNIESTKTNHYFKIIKEEINRMDKTVTDFLLTVRPIKIMPQKKDISDIIKSVYNLL
NPELKNKNIKFILSLKKVSFVLIDEKLIRQVIINIVKNAEEALLESNKKIKKIEISIHENENNIYVSIKDNANGITNETK
DDIFKPQFSTKENGSGIGLTISYKIVKEHGGEIFVESKEMKGTSFTITLPKLNKGKILIEGCLENE
>Mature_385_residues
SKFLKKTLTKLNKLSSDQKLKFIQDIYKKIEIYDGIFASINEGIMVLDKINNIIYLNKMLFQILSISPKYKLETLKDISI
PTLTNLIEELATNEDKIIGFEVQISTNIYIKISFMPYVRDQKLEGNIILIEDIKDKKHKEELFRRAEALAAFTRHARNIA
HEIKNPLGAIDINLQLLKKEIDKQNIESTKTNHYFKIIKEEINRMDKTVTDFLLTVRPIKIMPQKKDISDIIKSVYNLLN
PELKNKNIKFILSLKKVSFVLIDEKLIRQVIINIVKNAEEALLESNKKIKKIEISIHENENNIYVSIKDNANGITNETKD
DIFKPQFSTKENGSGIGLTISYKIVKEHGGEIFVESKEMKGTSFTITLPKLNKGKILIEGCLENE

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=27.3109243697479, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788549, Length=247, Percent_Identity=28.7449392712551, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1790300, Length=270, Percent_Identity=28.5185185185185, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48994928, Length=267, Percent_Identity=25.4681647940075, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788713, Length=234, Percent_Identity=26.4957264957265, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1786600, Length=346, Percent_Identity=21.9653179190751, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44499; Mature: 44368

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKFLKKTLTKLNKLSSDQKLKFIQDIYKKIEIYDGIFASINEGIMVLDKINNIIYLNKM
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCEEEEEHHCCEEHHHHH
LFQILSISPKYKLETLKDISIPTLTNLIEELATNEDKIIGFEVQISTNIYIKISFMPYVR
HHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEEEECEEEEEEEEECCCC
DQKLEGNIILIEDIKDKKHKEELFRRAEALAAFTRHARNIAHEIKNPLGAIDINLQLLKK
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHH
EIDKQNIESTKTNHYFKIIKEEINRMDKTVTDFLLTVRPIKIMPQKKDISDIIKSVYNLL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHH
NPELKNKNIKFILSLKKVSFVLIDEKLIRQVIINIVKNAEEALLESNKKIKKIEISIHEN
CCCCCCCCEEEEEEEEHHEEEEECHHHHHHHHHHHHHCHHHHHHHCCCCEEEEEEEEEEC
ENNIYVSIKDNANGITNETKDDIFKPQFSTKENGSGIGLTISYKIVKEHGGEIFVESKEM
CCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEEECCCC
KGTSFTITLPKLNKGKILIEGCLENE
CCCEEEEECCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SKFLKKTLTKLNKLSSDQKLKFIQDIYKKIEIYDGIFASINEGIMVLDKINNIIYLNKM
CHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCEEEEEHHCCEEHHHHH
LFQILSISPKYKLETLKDISIPTLTNLIEELATNEDKIIGFEVQISTNIYIKISFMPYVR
HHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEEEECEEEEEEEEECCCC
DQKLEGNIILIEDIKDKKHKEELFRRAEALAAFTRHARNIAHEIKNPLGAIDINLQLLKK
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHH
EIDKQNIESTKTNHYFKIIKEEINRMDKTVTDFLLTVRPIKIMPQKKDISDIIKSVYNLL
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHH
NPELKNKNIKFILSLKKVSFVLIDEKLIRQVIINIVKNAEEALLESNKKIKKIEISIHEN
CCCCCCCCEEEEEEEEHHEEEEECHHHHHHHHHHHHHCHHHHHHHCCCCEEEEEEEEEEC
ENNIYVSIKDNANGITNETKDDIFKPQFSTKENGSGIGLTISYKIVKEHGGEIFVESKEM
CCEEEEEEECCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEEEEEEEECCCCEEEEECCCC
KGTSFTITLPKLNKGKILIEGCLENE
CCCEEEEECCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]