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The map label for this gene is papS [H]

Identifier: 203284602

GI number: 203284602

Start: 753604

End: 754902

Strand: Reverse

Name: papS [H]

Synonym: BDU_708

Alternate gene names: 203284602

Gene position: 754902-753604 (Counterclockwise)

Preceding gene: 203284618

Following gene: 203284587

Centisome position: 81.03

GC content: 22.25

Gene sequence:

>1299_bases
ATGATATTTAAGAATAAACTAAAACCTTATCTTAACCTTAGAAAAAATAGTATTTCAACTATAATATATTTCTATATGAA
TCTAGGTAATAACACTAAAGATATAATAAAAATTAGTCAAATCTTTAATAACCATAACTATGAATTTTTCCTAGTAGGAG
GCGCATTAAGAGACTTACTTCTTAAAAAGATACCTTATGATTTTGATTTTACAACAAATGCAACTCCAAAAGAAATAATG
AAATTATTTCCAAAAAATATACAAACAGGAATAAAACATGGAACAATAAGTATAATCTTTAATAAAAAAATTTTTGAAAT
CACAACTTATAGAATAGACATAGATTATGAAAACAAACGCTCTCCTAAACAAATAAAATTTACAAAAAATTTAAAGGAAG
ACTTAAAAAGACGGGACTTCACAATAAATGCAATCGCAATGCATACACTTAACTTCGAAATAATAGATTACTACAATGGA
CAACAAGATCTTAATAAAAAAATAATAAAATGCATAGGAAATCCAAATAAACGATTTGAAGAAGATGCTCTTAGAATTCT
TAGAGCTGCAAGATTTGCATCCACACTGAATTTCACAATTGATAAGAATACATTAATTTCCATGAAATACAAAAAAAACA
ATATTTTATTTCTATCAAAAGAAAGAATCAATAATGAATTTATTAAACTTTTAGAGGGAGAAAATCCAACAAAGGGAATC
AAATATTTACAAAAAATAAATTTTTTTAAATACTTTTTTAACATAGAAATAAACAAAAAATTAATAAAAAAAATTACTCT
TTTAAATAAAAATAAATTTTATTTAAAAGCAATAGTTATTTTTACTATAAAACAAGACATATCAAATTTAAAACAAAACT
TAAAATTACTCAAGTTTTCAAACAAAGACATTAAACTAATATTATTATATAAAACAACTATCAACGCAATTCATAAATTA
AATCCAAAAAAATTACATCATATTAGAATTTTTTTAAGCAAAACTACCAGAGAAAATTATAAAGAAACACTTGATATATA
TAAAGCCCTTAAAGGAAAAAACAACAAGTTTAAATTTATAACAAACAAGATTAAAAATAATAAGCTGTTAAGAGATCCAT
TATCTTTAAAAGAATTGCAAATAAATGGCAACGATGTTCAAAAGTTCCAATTAAGAACCCACAAAAACACTGGTATAATT
CTCAATCATCTACTTAATGAAGTACTAATAGAGCCTAAATTAAATAAGAAAGAAATTTTGCTTCAAAAGATTAAAGATCA
ATATTCACAATATTTTTAA

Upstream 100 bases:

>100_bases
CTCTTGAAAGTTTATCAATAGAATCATATACACTAACATTACTCTTGTCCATAAAACCCCATTTATAAATATTTAATATA
AATCGAACAAATATTATTTT

Downstream 100 bases:

>100_bases
GTGCCATTTCAGCTGCTATCATCTCAGCCTCTTTTTTAGACTTTCCTTTGCCATTAGATACAAATTTATCATTAACATAA
AGTTCAACACAAAAAATCTT

Product: tRNA nucleotidyltransferase (CCA-adding enzyme)

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIFKNKLKPYLNLRKNSISTIIYFYMNLGNNTKDIIKISQIFNNHNYEFFLVGGALRDLLLKKIPYDFDFTTNATPKEIM
KLFPKNIQTGIKHGTISIIFNKKIFEITTYRIDIDYENKRSPKQIKFTKNLKEDLKRRDFTINAIAMHTLNFEIIDYYNG
QQDLNKKIIKCIGNPNKRFEEDALRILRAARFASTLNFTIDKNTLISMKYKKNNILFLSKERINNEFIKLLEGENPTKGI
KYLQKINFFKYFFNIEINKKLIKKITLLNKNKFYLKAIVIFTIKQDISNLKQNLKLLKFSNKDIKLILLYKTTINAIHKL
NPKKLHHIRIFLSKTTRENYKETLDIYKALKGKNNKFKFITNKIKNNKLLRDPLSLKELQINGNDVQKFQLRTHKNTGII
LNHLLNEVLIEPKLNKKEILLQKIKDQYSQYF

Sequences:

>Translated_432_residues
MIFKNKLKPYLNLRKNSISTIIYFYMNLGNNTKDIIKISQIFNNHNYEFFLVGGALRDLLLKKIPYDFDFTTNATPKEIM
KLFPKNIQTGIKHGTISIIFNKKIFEITTYRIDIDYENKRSPKQIKFTKNLKEDLKRRDFTINAIAMHTLNFEIIDYYNG
QQDLNKKIIKCIGNPNKRFEEDALRILRAARFASTLNFTIDKNTLISMKYKKNNILFLSKERINNEFIKLLEGENPTKGI
KYLQKINFFKYFFNIEINKKLIKKITLLNKNKFYLKAIVIFTIKQDISNLKQNLKLLKFSNKDIKLILLYKTTINAIHKL
NPKKLHHIRIFLSKTTRENYKETLDIYKALKGKNNKFKFITNKIKNNKLLRDPLSLKELQINGNDVQKFQLRTHKNTGII
LNHLLNEVLIEPKLNKKEILLQKIKDQYSQYF
>Mature_432_residues
MIFKNKLKPYLNLRKNSISTIIYFYMNLGNNTKDIIKISQIFNNHNYEFFLVGGALRDLLLKKIPYDFDFTTNATPKEIM
KLFPKNIQTGIKHGTISIIFNKKIFEITTYRIDIDYENKRSPKQIKFTKNLKEDLKRRDFTINAIAMHTLNFEIIDYYNG
QQDLNKKIIKCIGNPNKRFEEDALRILRAARFASTLNFTIDKNTLISMKYKKNNILFLSKERINNEFIKLLEGENPTKGI
KYLQKINFFKYFFNIEINKKLIKKITLLNKNKFYLKAIVIFTIKQDISNLKQNLKLLKFSNKDIKLILLYKTTINAIHKL
NPKKLHHIRIFLSKTTRENYKETLDIYKALKGKNNKFKFITNKIKNNKLLRDPLSLKELQINGNDVQKFQLRTHKNTGII
LNHLLNEVLIEPKLNKKEILLQKIKDQYSQYF

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=422, Percent_Identity=27.2511848341232, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI87081691, Length=232, Percent_Identity=33.1896551724138, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1789436, Length=216, Percent_Identity=31.4814814814815, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=281, Percent_Identity=28.8256227758007, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6321016, Length=227, Percent_Identity=28.6343612334802, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281360112, Length=230, Percent_Identity=33.4782608695652, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21357337, Length=230, Percent_Identity=33.4782608695652, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 51367; Mature: 51367

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFKNKLKPYLNLRKNSISTIIYFYMNLGNNTKDIIKISQIFNNHNYEFFLVGGALRDLL
CCCCHHCCHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECHHHHHHH
LKKIPYDFDFTTNATPKEIMKLFPKNIQTGIKHGTISIIFNKKIFEITTYRIDIDYENKR
HHHCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCCEEEEEECCEEEEEEEEEEEEEECCCC
SPKQIKFTKNLKEDLKRRDFTINAIAMHTLNFEIIDYYNGQQDLNKKIIKCIGNPNKRFE
CCCEEEEHHHHHHHHHHCCEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHCCCCCCHH
EDALRILRAARFASTLNFTIDKNTLISMKYKKNNILFLSKERINNEFIKLLEGENPTKGI
HHHHHHHHHHHHHHHEEEEEECCEEEEEEEECCCEEEEEHHHCCCHHHHHHCCCCCHHHH
KYLQKINFFKYFFNIEINKKLIKKITLLNKNKFYLKAIVIFTIKQDISNLKQNLKLLKFS
HHHHHHHHEEEEEEEEECHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHHCCEEEEEC
NKDIKLILLYKTTINAIHKLNPKKLHHIRIFLSKTTRENYKETLDIYKALKGKNNKFKFI
CCCEEEEEEEEHHHHHHHCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEE
TNKIKNNKLLRDPLSLKELQINGNDVQKFQLRTHKNTGIILNHLLNEVLIEPKLNKKEIL
EHHHCCCCEECCCCCCEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHH
LQKIKDQYSQYF
HHHHHHHHHCCC
>Mature Secondary Structure
MIFKNKLKPYLNLRKNSISTIIYFYMNLGNNTKDIIKISQIFNNHNYEFFLVGGALRDLL
CCCCHHCCHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECHHHHHHH
LKKIPYDFDFTTNATPKEIMKLFPKNIQTGIKHGTISIIFNKKIFEITTYRIDIDYENKR
HHHCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCCEEEEEECCEEEEEEEEEEEEEECCCC
SPKQIKFTKNLKEDLKRRDFTINAIAMHTLNFEIIDYYNGQQDLNKKIIKCIGNPNKRFE
CCCEEEEHHHHHHHHHHCCEEEEEEEEEEEEEEEEEEECCHHHHHHHHHHHHCCCCCCHH
EDALRILRAARFASTLNFTIDKNTLISMKYKKNNILFLSKERINNEFIKLLEGENPTKGI
HHHHHHHHHHHHHHHEEEEEECCEEEEEEEECCCEEEEEHHHCCCHHHHHHCCCCCHHHH
KYLQKINFFKYFFNIEINKKLIKKITLLNKNKFYLKAIVIFTIKQDISNLKQNLKLLKFS
HHHHHHHHEEEEEEEEECHHHHHHHHHCCCCCEEEEEEEEEEEHHHHHHHHHCCEEEEEC
NKDIKLILLYKTTINAIHKLNPKKLHHIRIFLSKTTRENYKETLDIYKALKGKNNKFKFI
CCCEEEEEEEEHHHHHHHCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEE
TNKIKNNKLLRDPLSLKELQINGNDVQKFQLRTHKNTGIILNHLLNEVLIEPKLNKKEIL
EHHHCCCCEECCCCCCEEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHH
LQKIKDQYSQYF
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]