Click here to switch to the map view.

The map label for this gene is ntrA [H]

Identifier: 203284360

GI number: 203284360

Start: 482927

End: 484183

Strand: Reverse

Name: ntrA [H]

Synonym: BDU_449

Alternate gene names: 203284360

Gene position: 484183-482927 (Counterclockwise)

Preceding gene: 203284361

Following gene: 203284359

Centisome position: 51.97

GC content: 24.42

Gene sequence:

>1257_bases
ATGCTAAAACAAAACTTAAAATTAACACACAAATTACAATTAACACAAATTAACACAATTAAAATGTTAAGTCTTGATAA
GCAAGAATTAATACAAATTATATCAGACGAACTTGACAATAATGAATATATTAAAGTGGATTCAAATAAAATCTTTTTTG
AATCACTAAAAACCTATAAGTTTAAAAAATTTTTTTATAAAGAAAATGAAAATAACAAAACACAATATGAAATTGCACTG
GCTAAAACATCACCTAAAATTTCACTTAAAGAACATCTTTTATTACAACTTAGAATCCAAAGACTTAATGAAGAAGAAAT
TAATATAGGAGAAATCATAATAAACAATCTAAATCGAAAAGGACTATACATAATCAATCCTTATGATTTCTTTAATAAAG
AAGAGTGGCCACAGGTCACTAAAATGATCAAATTGATTCAACAATTTGATCCAATAGGAATTTGCGTACCCAATATCATA
GAATCATTAATATTACAAGCAAAATACCATAACTTAGATACAAATATAATTAAAATTCTTCAAAAAGCAGATTTACTTAC
AAATACTAAAGAAAAATTACAAAAAGAATTAAACATTAGCACACAAGATTTAAATGACGCCATTAACACAATGAAACTTA
AACTTAATCCCAATCCAACATTTGAATTTAAAGACAAAGATGATACTAATGACTATATTGAACCAGATATTATTGTTATC
AATAAAGGCAATAAACTCAAAATCAAAATTAAAGAAGTCAGTATTTTTAAAACAGAAATTAATAAACAAGAAGTTAAAGA
CTTGTGCAAATACAAACAAGCAAAATGGCTCATTGAAGCTTTAAGATATAGAGATGAAACACTAGCTAAAATAGGAATAG
CAATATACACATTACAAAAAGAATTCTTAAAAAGAGGATTTAAGAGATTAAGACCAATGAACTTAGATGATATATCTACA
AGAATCAATCTATCAAAATCAACTATATCAAGAGCAATAAAAAATAAATATTTAAAATTTGACTGGGGCACAATAGCAAT
TAGAAAATTATTTAATTCAATAGGTGGTGCTAAAACAAATGAATTTTCCAAATTAAGCATTAAAATAATAATAAAAGGAA
TACTAGCACAGAATAAAAATATGGTAGATAGTCAAATTTCTGATATACTAAAATCTAAAGGAATCACTATTTCAAGAAGA
ACTGTAAACAAATATAGGAATGAATTAAAATGTGAAGGAGAAATTTATGGAACCTAA

Upstream 100 bases:

>100_bases
CACTTGCAGGATTAAACTTACTTATTTTATTATTCTACAAAAATGTTTCCCCTGCAATATTAATTATATCTAGTGGAGTA
CTATATATTTTTATGTAAAG

Downstream 100 bases:

>100_bases
AATACAAGCCATCAATTATCATTTAAATGATGATATGAAAGATTTTATTTTAAAAAAACTAGATAAAATTGGAACACACA
TTAAACAACATGCAGAAAGT

Product: RNA polymerase sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MLKQNLKLTHKLQLTQINTIKMLSLDKQELIQIISDELDNNEYIKVDSNKIFFESLKTYKFKKFFYKENENNKTQYEIAL
AKTSPKISLKEHLLLQLRIQRLNEEEINIGEIIINNLNRKGLYIINPYDFFNKEEWPQVTKMIKLIQQFDPIGICVPNII
ESLILQAKYHNLDTNIIKILQKADLLTNTKEKLQKELNISTQDLNDAINTMKLKLNPNPTFEFKDKDDTNDYIEPDIIVI
NKGNKLKIKIKEVSIFKTEINKQEVKDLCKYKQAKWLIEALRYRDETLAKIGIAIYTLQKEFLKRGFKRLRPMNLDDIST
RINLSKSTISRAIKNKYLKFDWGTIAIRKLFNSIGGAKTNEFSKLSIKIIIKGILAQNKNMVDSQISDILKSKGITISRR
TVNKYRNELKCEGEIYGT

Sequences:

>Translated_418_residues
MLKQNLKLTHKLQLTQINTIKMLSLDKQELIQIISDELDNNEYIKVDSNKIFFESLKTYKFKKFFYKENENNKTQYEIAL
AKTSPKISLKEHLLLQLRIQRLNEEEINIGEIIINNLNRKGLYIINPYDFFNKEEWPQVTKMIKLIQQFDPIGICVPNII
ESLILQAKYHNLDTNIIKILQKADLLTNTKEKLQKELNISTQDLNDAINTMKLKLNPNPTFEFKDKDDTNDYIEPDIIVI
NKGNKLKIKIKEVSIFKTEINKQEVKDLCKYKQAKWLIEALRYRDETLAKIGIAIYTLQKEFLKRGFKRLRPMNLDDIST
RINLSKSTISRAIKNKYLKFDWGTIAIRKLFNSIGGAKTNEFSKLSIKIIIKGILAQNKNMVDSQISDILKSKGITISRR
TVNKYRNELKCEGEIYGT
>Mature_418_residues
MLKQNLKLTHKLQLTQINTIKMLSLDKQELIQIISDELDNNEYIKVDSNKIFFESLKTYKFKKFFYKENENNKTQYEIAL
AKTSPKISLKEHLLLQLRIQRLNEEEINIGEIIINNLNRKGLYIINPYDFFNKEEWPQVTKMIKLIQQFDPIGICVPNII
ESLILQAKYHNLDTNIIKILQKADLLTNTKEKLQKELNISTQDLNDAINTMKLKLNPNPTFEFKDKDDTNDYIEPDIIVI
NKGNKLKIKIKEVSIFKTEINKQEVKDLCKYKQAKWLIEALRYRDETLAKIGIAIYTLQKEFLKRGFKRLRPMNLDDIST
RINLSKSTISRAIKNKYLKFDWGTIAIRKLFNSIGGAKTNEFSKLSIKIIIKGILAQNKNMVDSQISDILKSKGITISRR
TVNKYRNELKCEGEIYGT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen utilization genes [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=352, Percent_Identity=25.5681818181818, Blast_Score=122, Evalue=5e-29,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 48917; Mature: 48917

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQNLKLTHKLQLTQINTIKMLSLDKQELIQIISDELDNNEYIKVDSNKIFFESLKTYK
CCCCCCCEEEEEEEEEECEEEEECCCHHHHHHHHHHHCCCCCEEEECCCEEEHHHHHHHH
FKKFFYKENENNKTQYEIALAKTSPKISLKEHLLLQLRIQRLNEEEINIGEIIINNLNRK
HHHHHEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCC
GLYIINPYDFFNKEEWPQVTKMIKLIQQFDPIGICVPNIIESLILQAKYHNLDTNIIKIL
CEEEECCHHHCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHH
QKADLLTNTKEKLQKELNISTQDLNDAINTMKLKLNPNPTFEFKDKDDTNDYIEPDIIVI
HHHHHHCCHHHHHHHHHCCCHHHHHHHHHEEEEEECCCCCEEECCCCCCCCCCCCCEEEE
NKGNKLKIKIKEVSIFKTEINKQEVKDLCKYKQAKWLIEALRYRDETLAKIGIAIYTLQK
ECCCEEEEEEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLKRGFKRLRPMNLDDISTRINLSKSTISRAIKNKYLKFDWGTIAIRKLFNSIGGAKTN
HHHHHHHHHCCCCCCCHHHHEEECCHHHHHHHHHHCCEEECHHHHHHHHHHHHHCCCCCC
EFSKLSIKIIIKGILAQNKNMVDSQISDILKSKGITISRRTVNKYRNELKCEGEIYGT
CCHHEEHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHCEECCEECCC
>Mature Secondary Structure
MLKQNLKLTHKLQLTQINTIKMLSLDKQELIQIISDELDNNEYIKVDSNKIFFESLKTYK
CCCCCCCEEEEEEEEEECEEEEECCCHHHHHHHHHHHCCCCCEEEECCCEEEHHHHHHHH
FKKFFYKENENNKTQYEIALAKTSPKISLKEHLLLQLRIQRLNEEEINIGEIIINNLNRK
HHHHHEECCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCC
GLYIINPYDFFNKEEWPQVTKMIKLIQQFDPIGICVPNIIESLILQAKYHNLDTNIIKIL
CEEEECCHHHCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHH
QKADLLTNTKEKLQKELNISTQDLNDAINTMKLKLNPNPTFEFKDKDDTNDYIEPDIIVI
HHHHHHCCHHHHHHHHHCCCHHHHHHHHHEEEEEECCCCCEEECCCCCCCCCCCCCEEEE
NKGNKLKIKIKEVSIFKTEINKQEVKDLCKYKQAKWLIEALRYRDETLAKIGIAIYTLQK
ECCCEEEEEEEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLKRGFKRLRPMNLDDISTRINLSKSTISRAIKNKYLKFDWGTIAIRKLFNSIGGAKTN
HHHHHHHHHCCCCCCCHHHHEEECCHHHHHHHHHHCCEEECHHHHHHHHHHHHHCCCCCC
EFSKLSIKIIIKGILAQNKNMVDSQISDILKSKGITISRRTVNKYRNELKCEGEIYGT
CCHHEEHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEHHHHHHHHHHCEECCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969534 [H]