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The map label for this gene is fruA-1 [H]

Identifier: 203284318

GI number: 203284318

Start: 431385

End: 433253

Strand: Reverse

Name: fruA-1 [H]

Synonym: BDU_402

Alternate gene names: 203284318

Gene position: 433253-431385 (Counterclockwise)

Preceding gene: 203284322

Following gene: 203284317

Centisome position: 46.5

GC content: 34.3

Gene sequence:

>1869_bases
ATGTTTTTGGATTTTTTAAAAAAGGAGCTTATTTTTGTATCGTCCACAATATGTTCTAAAGATGAAGCACTTAATTTTTT
AGTGGAAGAAGTAAGTAAGAAAGGTTATACAAATGATAAATCAGTATTTATGCAAGGATTGCTTGATAGGGAAAATATTG
GTGATACATCTTGGGAAAATGGAGTTGCTATTCCTCATTTTGTAGGAGATGTTGTTAGTATCAGTTTTATTTCATTGCTT
TACATAAAGGGTAATGGTATAAAATGGTCTGATGATAATCCTCCTGTTAATTTAATATTTTTAATTTGTATGTCAAAGGA
TCAACAAGGTACTGGGCATATTAAGTCAATAGCTTTTATAGCCAGATTATTTGAAAATGATGATTTTAAAAATTTTTTAA
AAAATGGAAATAATTCTGATGAAATTTATTCTTATATAAAAAATGTTGAAAATAATTATTTAGAGAGTGATTTTAGTATT
TCTAGGACAGAGAATATATTGGCTGTATGTGCTTGTCCTGTAGGAATTGCACATACATATATTGCTGCTAAGAAGCTTGA
AGTTGAAGCTAAAAGACAGGGATATAGCATTAAAGTTGAAACTCAGGGTTCTATTGGTATTGATAATCCTTTAAAAAAAG
AGGATATTGAAGCTGCTGATGTTGTAATACTTGCAGTAGATAAAGATGTTGATGAGTTAAGATTTGATGGAAAGCGAGTT
TATAAAATTTCGACTGCAAGGGCTATTAACGATGTAGAGAATGTTATTAAAGAAGCATTTAGTGTTCCAGTGTTAACATT
TAAAGGTATGAATACTTTAAAGGATAATTCTAATAATAGCAAATCTGGCTTTTACAAATATTTAATGAGTGGTGTCTCTC
CTATGGTTCCAATTGTGGCTAGTGGTGGTATTTTAATAGCCTTTGGCATATCTCTTGCAGGGGTTGGTTCTGGTGGTCCA
AATTTTGAGCAATATCCTTTTTATAAGACAATTACAGATATTGGTGGTGTGGCTTTTAGTATGATGTTGCCAGTACTTTC
AGGATTTATTGCAATGTCAATTGCTGATAAGCCAGGTCTTGCACCAGGTCTTGTGGGAGGAGTTCTTGCTAGGGACGTTA
AAGCAGGATTTTTAGGAGCCATACTTGTAGGGTTTATGGCAGGTTTTATTGCAAGATGGTTAGCAAGAAGACAAATATCT
GAATGGCTTAGGCCTATAATGCCTATATTTGTAATTCCTTTAATAAGTACTGTTATTATTGGATTTTTCATGATTTACGG
AGGTGTTTATATTGCCCAATTTATGGGACTTCTTGAAAATGGTCTTAAGGAGCTTCAAAGTAATTCAGATGCTCATGGTA
TTATAGGAAAGTTGTTACTTGGGGCAGTACTTGGTGCTATGGTATCAATTGATATGGGAGGGCCTTTTAATAAAGTTGCA
TTTCTTTTTGGTGTTGGTATGATTCCTAGTGTGCCACAAATAATGGGTATGGTTGCATCAGCTATTCCTGTTGCTCCTAT
GGCTATGGGACTTGCTACTTTGATTATGCCACAATTATTTGAAGAGGAAGAGAGAGAATCGGGAAAAATATCATTTTTGA
TTTCTTTTATTGGCATTAGTGAGGGTGCTATTCCTTTTGCTGCCAGTGATCCTGCAAGAGTTTTGCCTTCGATAGTGCTT
GGAGGTGCTGTTTCAAGCATTATTGCAGCATTTTGGGGCGTTGCAGATCATGCTCCGCATGGGGGGCCAATAGTTTTACC
TGTTGTTGATCATAAATTTGGATTTCTTGTTGCAATAGCTGTTGGTGTTGCGGTGGCAACAAGTGTAGTTATTTTTTTGA
AATCTTTAAAGGTTAAGGTATCTAAATGA

Upstream 100 bases:

>100_bases
CAATTATTTGCAAATAAAGAAGTGTTAGTGAGATGGGGAATACAAATTTAATCTTTGTACTGTGATAAAATTAATTTAAG
TACTTAGGAGGTTTAAGGTT

Downstream 100 bases:

>100_bases
GTGTTGATAATATATTTTTGATGAAAAATGAGATTAAGGAGTATGATTGGGGTGGATATGGTTTCATCCCTTCCCTTTTG
GGACAAAAAAAAGACGGTTT

Product: PTS system, fructose-specific IIABC component

Products: NA

Alternate protein names: EIIBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MFLDFLKKELIFVSSTICSKDEALNFLVEEVSKKGYTNDKSVFMQGLLDRENIGDTSWENGVAIPHFVGDVVSISFISLL
YIKGNGIKWSDDNPPVNLIFLICMSKDQQGTGHIKSIAFIARLFENDDFKNFLKNGNNSDEIYSYIKNVENNYLESDFSI
SRTENILAVCACPVGIAHTYIAAKKLEVEAKRQGYSIKVETQGSIGIDNPLKKEDIEAADVVILAVDKDVDELRFDGKRV
YKISTARAINDVENVIKEAFSVPVLTFKGMNTLKDNSNNSKSGFYKYLMSGVSPMVPIVASGGILIAFGISLAGVGSGGP
NFEQYPFYKTITDIGGVAFSMMLPVLSGFIAMSIADKPGLAPGLVGGVLARDVKAGFLGAILVGFMAGFIARWLARRQIS
EWLRPIMPIFVIPLISTVIIGFFMIYGGVYIAQFMGLLENGLKELQSNSDAHGIIGKLLLGAVLGAMVSIDMGGPFNKVA
FLFGVGMIPSVPQIMGMVASAIPVAPMAMGLATLIMPQLFEEEERESGKISFLISFIGISEGAIPFAASDPARVLPSIVL
GGAVSSIIAAFWGVADHAPHGGPIVLPVVDHKFGFLVAIAVGVAVATSVVIFLKSLKVKVSK

Sequences:

>Translated_622_residues
MFLDFLKKELIFVSSTICSKDEALNFLVEEVSKKGYTNDKSVFMQGLLDRENIGDTSWENGVAIPHFVGDVVSISFISLL
YIKGNGIKWSDDNPPVNLIFLICMSKDQQGTGHIKSIAFIARLFENDDFKNFLKNGNNSDEIYSYIKNVENNYLESDFSI
SRTENILAVCACPVGIAHTYIAAKKLEVEAKRQGYSIKVETQGSIGIDNPLKKEDIEAADVVILAVDKDVDELRFDGKRV
YKISTARAINDVENVIKEAFSVPVLTFKGMNTLKDNSNNSKSGFYKYLMSGVSPMVPIVASGGILIAFGISLAGVGSGGP
NFEQYPFYKTITDIGGVAFSMMLPVLSGFIAMSIADKPGLAPGLVGGVLARDVKAGFLGAILVGFMAGFIARWLARRQIS
EWLRPIMPIFVIPLISTVIIGFFMIYGGVYIAQFMGLLENGLKELQSNSDAHGIIGKLLLGAVLGAMVSIDMGGPFNKVA
FLFGVGMIPSVPQIMGMVASAIPVAPMAMGLATLIMPQLFEEEERESGKISFLISFIGISEGAIPFAASDPARVLPSIVL
GGAVSSIIAAFWGVADHAPHGGPIVLPVVDHKFGFLVAIAVGVAVATSVVIFLKSLKVKVSK
>Mature_622_residues
MFLDFLKKELIFVSSTICSKDEALNFLVEEVSKKGYTNDKSVFMQGLLDRENIGDTSWENGVAIPHFVGDVVSISFISLL
YIKGNGIKWSDDNPPVNLIFLICMSKDQQGTGHIKSIAFIARLFENDDFKNFLKNGNNSDEIYSYIKNVENNYLESDFSI
SRTENILAVCACPVGIAHTYIAAKKLEVEAKRQGYSIKVETQGSIGIDNPLKKEDIEAADVVILAVDKDVDELRFDGKRV
YKISTARAINDVENVIKEAFSVPVLTFKGMNTLKDNSNNSKSGFYKYLMSGVSPMVPIVASGGILIAFGISLAGVGSGGP
NFEQYPFYKTITDIGGVAFSMMLPVLSGFIAMSIADKPGLAPGLVGGVLARDVKAGFLGAILVGFMAGFIARWLARRQIS
EWLRPIMPIFVIPLISTVIIGFFMIYGGVYIAQFMGLLENGLKELQSNSDAHGIIGKLLLGAVLGAMVSIDMGGPFNKVA
FLFGVGMIPSVPQIMGMVASAIPVAPMAMGLATLIMPQLFEEEERESGKISFLISFIGISEGAIPFAASDPARVLPSIVL
GGAVSSIIAAFWGVADHAPHGGPIVLPVVDHKFGFLVAIAVGVAVATSVVIFLKSLKVKVSK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=472, Percent_Identity=39.6186440677966, Blast_Score=324, Evalue=1e-89,
Organism=Escherichia coli, GI1786951, Length=636, Percent_Identity=28.3018867924528, Blast_Score=258, Evalue=1e-69,
Organism=Escherichia coli, GI87082348, Length=481, Percent_Identity=32.6403326403326, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1790386, Length=354, Percent_Identity=38.9830508474576, Blast_Score=216, Evalue=5e-57,
Organism=Escherichia coli, GI1788729, Length=398, Percent_Identity=27.6381909547739, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1790387, Length=94, Percent_Identity=45.7446808510638, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1788730, Length=98, Percent_Identity=42.8571428571429, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790390, Length=105, Percent_Identity=35.2380952380952, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 66877; Mature: 66877

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLDFLKKELIFVSSTICSKDEALNFLVEEVSKKGYTNDKSVFMQGLLDRENIGDTSWEN
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCC
GVAIPHFVGDVVSISFISLLYIKGNGIKWSDDNPPVNLIFLICMSKDQQGTGHIKSIAFI
CCCCHHHHHHHHHHHHHHEEEEECCCEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHH
ARLFENDDFKNFLKNGNNSDEIYSYIKNVENNYLESDFSISRTENILAVCACPVGIAHTY
HHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEEEECCHHHHHHH
IAAKKLEVEAKRQGYSIKVETQGSIGIDNPLKKEDIEAADVVILAVDKDVDELRFDGKRV
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCEE
YKISTARAINDVENVIKEAFSVPVLTFKGMNTLKDNSNNSKSGFYKYLMSGVSPMVPIVA
EEEHHHHHHHHHHHHHHHHHCCCCEEECCCCHHCCCCCCCHHHHHHHHHHCCCCCHHHHH
SGGILIAFGISLAGVGSGGPNFEQYPFYKTITDIGGVAFSMMLPVLSGFIAMSIADKPGL
CCCEEEEECCHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
APGLVGGVLARDVKAGFLGAILVGFMAGFIARWLARRQISEWLRPIMPIFVIPLISTVII
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFFMIYGGVYIAQFMGLLENGLKELQSNSDAHGIIGKLLLGAVLGAMVSIDMGGPFNKVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHH
FLFGVGMIPSVPQIMGMVASAIPVAPMAMGLATLIMPQLFEEEERESGKISFLISFIGIS
HHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
EGAIPFAASDPARVLPSIVLGGAVSSIIAAFWGVADHAPHGGPIVLPVVDHKFGFLVAIA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHH
VGVAVATSVVIFLKSLKVKVSK
HHHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure
MFLDFLKKELIFVSSTICSKDEALNFLVEEVSKKGYTNDKSVFMQGLLDRENIGDTSWEN
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCC
GVAIPHFVGDVVSISFISLLYIKGNGIKWSDDNPPVNLIFLICMSKDQQGTGHIKSIAFI
CCCCHHHHHHHHHHHHHHEEEEECCCEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHH
ARLFENDDFKNFLKNGNNSDEIYSYIKNVENNYLESDFSISRTENILAVCACPVGIAHTY
HHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCEEEEEECCHHHHHHH
IAAKKLEVEAKRQGYSIKVETQGSIGIDNPLKKEDIEAADVVILAVDKDVDELRFDGKRV
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCEE
YKISTARAINDVENVIKEAFSVPVLTFKGMNTLKDNSNNSKSGFYKYLMSGVSPMVPIVA
EEEHHHHHHHHHHHHHHHHHCCCCEEECCCCHHCCCCCCCHHHHHHHHHHCCCCCHHHHH
SGGILIAFGISLAGVGSGGPNFEQYPFYKTITDIGGVAFSMMLPVLSGFIAMSIADKPGL
CCCEEEEECCHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
APGLVGGVLARDVKAGFLGAILVGFMAGFIARWLARRQISEWLRPIMPIFVIPLISTVII
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFFMIYGGVYIAQFMGLLENGLKELQSNSDAHGIIGKLLLGAVLGAMVSIDMGGPFNKVA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHHH
FLFGVGMIPSVPQIMGMVASAIPVAPMAMGLATLIMPQLFEEEERESGKISFLISFIGIS
HHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
EGAIPFAASDPARVLPSIVLGGAVSSIIAAFWGVADHAPHGGPIVLPVVDHKFGFLVAIA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHH
VGVAVATSVVIFLKSLKVKVSK
HHHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3076173; 9278503; 9097040; 1981619 [H]