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The map label for this gene is proS [H]

Identifier: 203284314

GI number: 203284314

Start: 426511

End: 427986

Strand: Reverse

Name: proS [H]

Synonym: BDU_398

Alternate gene names: 203284314

Gene position: 427986-426511 (Counterclockwise)

Preceding gene: 203284317

Following gene: 203284310

Centisome position: 45.94

GC content: 30.89

Gene sequence:

>1476_bases
GTGTGGTTTATGAGTGATTTTATTGCTTCAAGAGAAGAGGAATTTTCTAAATGGTACTTGGATATAGTACAAAAGGCAAA
ACTTGTTGATTATAGTCCTGTTAAGGGTTGTATGGTTATTATGCCTTATGGATATGCTATTTGGGAGAGAATTCAGAGTG
TAATTGATGATAAGTTTAAAGAGAGTGGGCATGAGAATGCATATTTTCCATTACTGATTCCTTATGAGTTTTTAGAGAGG
GAAAAAGAGCATGTTAAGGGATTCTCTCCAGAACTTGCTGTTGTAACAACAGCTGGGGGTGAAGAATTGAGAGAACCTTT
GGTTTTAAGACCTACTTCTGAGACAATTATTTGGAATATGTATAGTAAATGGATAAAATCTTATAGAGATTTACCTATTA
AAATAAATCAATGGGCAAATATTATTCGTTGGGAAAAAAGGACAAAGCCATTTCTTCGTACTACTGAGTTTTTGTGGCAA
GAGGGACATACTGCTCATGAGACTTCTAATGAAGCTCAAGAGGAAGCTTTGTTTATTTTAAATCTTTATAAAAAATTTGT
TGAAGATTATTTGGCTATTCCTGTATTTTATGGTAAGAAGACAGAAAGAGAAAAATTTGCAGGTGCTGTGTCTACTTATA
CAATTGAAGCGTTGATGCAAGATAAAAAAGCTTTGCAAGCAGGAACATCACATTATTTGGGATTGAATTTTGCCAAGGCT
TTTGATGTTAAATTTCAAAATAAAAACGGTGAGATGAGTTATGTTTTTGCCACTAGTTGGGGGGTTTCAACTAGGTTAAT
TGGGGCATTGATTATGGTTCATTCTGATTCTAAAGGTTTGATTTTACCTCCTAAGATAGCACCAATAGAAATTCTTATTG
TTCCTATTTTTAAAAAAGATGATGATGTTAATAAAAAAATTCTTGAATATGCGACTACTATTTTTGATATTTTAGTAAAG
GAAAAGTTTAGGGTTGAGATTGATAAAGATTTTAAGAATTCACCAGGATTTAGATTTGCTGCTTCAGAATTTAAGGGGAT
TCCAATACGAATTGAGATAGGTTCTAATGATATTTTAATGGATTGTGTCACTGTTGCAAGAAGAGATAGAGATAAAAATT
CTAAGTATCAAGTATCATTTAAAGAGTTATTACCTAAAATAAAAGAGGAACTTGAGACTATGCAATGTGAATTATTTAAT
AAAGCGTTAGAATTTAGAAATTCAAATACCAAAGAAATTATTGGTTTTAAAGAGAATGATTATGATCTTTTTAAGACTTA
TATTAACGATAATTTAGGATTTGTACTCTCTTCATGGTGTGGAAATGAAGTTTGTGAAGAAAATATTAAAAATGATACAA
AGGCTACAATACGATGTATTCCAGAAGATTTTCAAGATAAATCTTTAGACAATGTAACTTGTATTTATTGCAATACATCA
GCCAAGCATTTTGTTTTATTTGCAAGAGCTTATTAA

Upstream 100 bases:

>100_bases
ATTAATATATATGATATCCTTTATTGTAAAAAGTAGATTTTTGCTTTTGAGGTCAAATTATATTGTGTCTTTATAGTTAA
GTTTTAAGTTTTTAGGGAAG

Downstream 100 bases:

>100_bases
ATTTTTATTTTTTAGTTATTAAGATATTAAGTATATTGTGAGTCATTAAAGTCTATTATTTGCATTATTGCAATGCTTGA
GGTAAAGTCAATCATTATAG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MWFMSDFIASREEEFSKWYLDIVQKAKLVDYSPVKGCMVIMPYGYAIWERIQSVIDDKFKESGHENAYFPLLIPYEFLER
EKEHVKGFSPELAVVTTAGGEELREPLVLRPTSETIIWNMYSKWIKSYRDLPIKINQWANIIRWEKRTKPFLRTTEFLWQ
EGHTAHETSNEAQEEALFILNLYKKFVEDYLAIPVFYGKKTEREKFAGAVSTYTIEALMQDKKALQAGTSHYLGLNFAKA
FDVKFQNKNGEMSYVFATSWGVSTRLIGALIMVHSDSKGLILPPKIAPIEILIVPIFKKDDDVNKKILEYATTIFDILVK
EKFRVEIDKDFKNSPGFRFAASEFKGIPIRIEIGSNDILMDCVTVARRDRDKNSKYQVSFKELLPKIKEELETMQCELFN
KALEFRNSNTKEIIGFKENDYDLFKTYINDNLGFVLSSWCGNEVCEENIKNDTKATIRCIPEDFQDKSLDNVTCIYCNTS
AKHFVLFARAY

Sequences:

>Translated_491_residues
MWFMSDFIASREEEFSKWYLDIVQKAKLVDYSPVKGCMVIMPYGYAIWERIQSVIDDKFKESGHENAYFPLLIPYEFLER
EKEHVKGFSPELAVVTTAGGEELREPLVLRPTSETIIWNMYSKWIKSYRDLPIKINQWANIIRWEKRTKPFLRTTEFLWQ
EGHTAHETSNEAQEEALFILNLYKKFVEDYLAIPVFYGKKTEREKFAGAVSTYTIEALMQDKKALQAGTSHYLGLNFAKA
FDVKFQNKNGEMSYVFATSWGVSTRLIGALIMVHSDSKGLILPPKIAPIEILIVPIFKKDDDVNKKILEYATTIFDILVK
EKFRVEIDKDFKNSPGFRFAASEFKGIPIRIEIGSNDILMDCVTVARRDRDKNSKYQVSFKELLPKIKEELETMQCELFN
KALEFRNSNTKEIIGFKENDYDLFKTYINDNLGFVLSSWCGNEVCEENIKNDTKATIRCIPEDFQDKSLDNVTCIYCNTS
AKHFVLFARAY
>Mature_491_residues
MWFMSDFIASREEEFSKWYLDIVQKAKLVDYSPVKGCMVIMPYGYAIWERIQSVIDDKFKESGHENAYFPLLIPYEFLER
EKEHVKGFSPELAVVTTAGGEELREPLVLRPTSETIIWNMYSKWIKSYRDLPIKINQWANIIRWEKRTKPFLRTTEFLWQ
EGHTAHETSNEAQEEALFILNLYKKFVEDYLAIPVFYGKKTEREKFAGAVSTYTIEALMQDKKALQAGTSHYLGLNFAKA
FDVKFQNKNGEMSYVFATSWGVSTRLIGALIMVHSDSKGLILPPKIAPIEILIVPIFKKDDDVNKKILEYATTIFDILVK
EKFRVEIDKDFKNSPGFRFAASEFKGIPIRIEIGSNDILMDCVTVARRDRDKNSKYQVSFKELLPKIKEELETMQCELFN
KALEFRNSNTKEIIGFKENDYDLFKTYINDNLGFVLSSWCGNEVCEENIKNDTKATIRCIPEDFQDKSLDNVTCIYCNTS
AKHFVLFARAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=503, Percent_Identity=39.7614314115308, Blast_Score=370, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71991342, Length=497, Percent_Identity=39.6378269617706, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71991346, Length=352, Percent_Identity=44.0340909090909, Blast_Score=341, Evalue=7e-94,
Organism=Saccharomyces cerevisiae, GI6321808, Length=504, Percent_Identity=42.6587301587302, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24649468, Length=504, Percent_Identity=38.0952380952381, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24649466, Length=504, Percent_Identity=38.0952380952381, Blast_Score=359, Evalue=2e-99,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 57085; Mature: 57085

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWFMSDFIASREEEFSKWYLDIVQKAKLVDYSPVKGCMVIMPYGYAIWERIQSVIDDKFK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
ESGHENAYFPLLIPYEFLEREKEHVKGFSPELAVVTTAGGEELREPLVLRPTSETIIWNM
HCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCEEECCCCCCEEHHH
YSKWIKSYRDLPIKINQWANIIRWEKRTKPFLRTTEFLWQEGHTAHETSNEAQEEALFIL
HHHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
NLYKKFVEDYLAIPVFYGKKTEREKFAGAVSTYTIEALMQDKKALQAGTSHYLGLNFAKA
HHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEE
FDVKFQNKNGEMSYVFATSWGVSTRLIGALIMVHSDSKGLILPPKIAPIEILIVPIFKKD
EEEEEECCCCCEEEEEEECCCHHHHHHHEEEEEEECCCCEEECCCCCCEEEEEEEEECCC
DDVNKKILEYATTIFDILVKEKFRVEIDKDFKNSPGFRFAASEFKGIPIRIEIGSNDILM
CCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEHHHCCCCEEEEEECCCCHHH
DCVTVARRDRDKNSKYQVSFKELLPKIKEELETMQCELFNKALEFRNSNTKEIIGFKEND
HHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
YDLFKTYINDNLGFVLSSWCGNEVCEENIKNDTKATIRCIPEDFQDKSLDNVTCIYCNTS
HHHHHHHHCCCHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEEECCC
AKHFVLFARAY
CCEEEEEEECC
>Mature Secondary Structure
MWFMSDFIASREEEFSKWYLDIVQKAKLVDYSPVKGCMVIMPYGYAIWERIQSVIDDKFK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
ESGHENAYFPLLIPYEFLEREKEHVKGFSPELAVVTTAGGEELREPLVLRPTSETIIWNM
HCCCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEEEECCCHHHCCCEEECCCCCCEEHHH
YSKWIKSYRDLPIKINQWANIIRWEKRTKPFLRTTEFLWQEGHTAHETSNEAQEEALFIL
HHHHHHHHCCCCEEEHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
NLYKKFVEDYLAIPVFYGKKTEREKFAGAVSTYTIEALMQDKKALQAGTSHYLGLNFAKA
HHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEE
FDVKFQNKNGEMSYVFATSWGVSTRLIGALIMVHSDSKGLILPPKIAPIEILIVPIFKKD
EEEEEECCCCCEEEEEEECCCHHHHHHHEEEEEEECCCCEEECCCCCCEEEEEEEEECCC
DDVNKKILEYATTIFDILVKEKFRVEIDKDFKNSPGFRFAASEFKGIPIRIEIGSNDILM
CCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEHHHCCCCEEEEEECCCCHHH
DCVTVARRDRDKNSKYQVSFKELLPKIKEELETMQCELFNKALEFRNSNTKEIIGFKEND
HHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
YDLFKTYINDNLGFVLSSWCGNEVCEENIKNDTKATIRCIPEDFQDKSLDNVTCIYCNTS
HHHHHHHHCCCHHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEEECCC
AKHFVLFARAY
CCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA