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The map label for this gene is rsmE2 [H]

Identifier: 203284273

GI number: 203284273

Start: 380550

End: 381278

Strand: Reverse

Name: rsmE2 [H]

Synonym: BDU_355

Alternate gene names: 203284273

Gene position: 381278-380550 (Counterclockwise)

Preceding gene: 203284274

Following gene: 203284272

Centisome position: 40.92

GC content: 26.89

Gene sequence:

>729_bases
GTGAAACAAATAGTTTTAGATTATAGTTGTCTATTTGATGATGACATCATTATTGATGATATTAAGATTTATCACTATCT
TGTTAATGTAAGAAGATTAAGGGTAGGTGATAGATTGAATATTCTTTTAGGTAAAAGAGAGATAAGATTTTCTGAAATTT
TGAGTATAGAAAATCACCGAATTAGGTTTGCTACTCTTAGAGTAGAGGAGCTTAAGATGCGTGACTTTGCAATAGATATA
TTTATATCAAATCTTAAGGGTAGGAAATTAGATTTAAGTTTAAGACAAGTTGTTGAGATTGGTGTTAATAGTATAAATAT
TGTTAATGCTGATAATTCTATTGCTAAGCTAGATATGGATAATTTAGAACCAAAGAAATTAAGATTTTTAAAGATAATAG
ATGAGGCTTTAAAACAAAGTGGAAATGCTCAAATTCCCAGTATTAATTTTTATAATGATTTTTTTAGTATTCCTTATTCG
TCATCTGTGAATTATTACGTTGCTTATAGTAAGGGTGATTTTTTGCATTTGGGTGAAAACATTAGTGTTTTTGGTAAAAT
TGGGATTTTAATAGGTCCTGAGGGTTGTTTTTTAAAGTTGGAAATTGATTTTTTTAAAAAATTGAATTTTAAATTTGTAA
GATTTAATACTCCTATTTTAAGAGCTGATACAGCTATTGTTTATGCACTTGCTCACTTTAAATTATTATTAGAGGGTAAT
AATGGCTAA

Upstream 100 bases:

>100_bases
AGATATACACCATAATCAGCCAATTTATAATTTATATTATGATGAAGTCTTGAAAACTGCTTATGAATATCTTGTAAAGA
AAGGGACACAAAATTAATTT

Downstream 100 bases:

>100_bases
TTTAAAAGATATATATTCAAAACCAGATAGGATTTATTTTCTTGGAGCGCCAATAGATGTGTTTAAAAATCATGAAAAAT
TGATTAGTAGATTTGAATAT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase 2 [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MKQIVLDYSCLFDDDIIIDDIKIYHYLVNVRRLRVGDRLNILLGKREIRFSEILSIENHRIRFATLRVEELKMRDFAIDI
FISNLKGRKLDLSLRQVVEIGVNSINIVNADNSIAKLDMDNLEPKKLRFLKIIDEALKQSGNAQIPSINFYNDFFSIPYS
SSVNYYVAYSKGDFLHLGENISVFGKIGILIGPEGCFLKLEIDFFKKLNFKFVRFNTPILRADTAIVYALAHFKLLLEGN
NG

Sequences:

>Translated_242_residues
MKQIVLDYSCLFDDDIIIDDIKIYHYLVNVRRLRVGDRLNILLGKREIRFSEILSIENHRIRFATLRVEELKMRDFAIDI
FISNLKGRKLDLSLRQVVEIGVNSINIVNADNSIAKLDMDNLEPKKLRFLKIIDEALKQSGNAQIPSINFYNDFFSIPYS
SSVNYYVAYSKGDFLHLGENISVFGKIGILIGPEGCFLKLEIDFFKKLNFKFVRFNTPILRADTAIVYALAHFKLLLEGN
NG
>Mature_242_residues
MKQIVLDYSCLFDDDIIIDDIKIYHYLVNVRRLRVGDRLNILLGKREIRFSEILSIENHRIRFATLRVEELKMRDFAIDI
FISNLKGRKLDLSLRQVVEIGVNSINIVNADNSIAKLDMDNLEPKKLRFLKIIDEALKQSGNAQIPSINFYNDFFSIPYS
SSVNYYVAYSKGDFLHLGENISVFGKIGILIGPEGCFLKLEIDFFKKLNFKFVRFNTPILRADTAIVYALAHFKLLLEGN
NG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27918; Mature: 27918

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQIVLDYSCLFDDDIIIDDIKIYHYLVNVRRLRVGDRLNILLGKREIRFSEILSIENHR
CCEEEEEEEEEECCCEEEEHHHHHHHHHHHEEEECCCEEEEEEECCCCCHHHHHCCCCCE
IRFATLRVEELKMRDFAIDIFISNLKGRKLDLSLRQVVEIGVNSINIVNADNSIAKLDMD
EEEEEEEEEHHHHEEEEEEEEEECCCCCEECHHHHHHHHHCCCEEEEEECCCCEEEEECC
NLEPKKLRFLKIIDEALKQSGNAQIPSINFYNDFFSIPYSSSVNYYVAYSKGDFLHLGEN
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHCCCCCCCCCCEEEEECCCCEEEECCC
ISVFGKIGILIGPEGCFLKLEIDFFKKLNFKFVRFNTPILRADTAIVYALAHFKLLLEGN
EEEEEEEEEEECCCCCEEEEEEEHHHCCCEEEEEECCCEEECCHHHHEEEEEEEEEEECC
NG
CC
>Mature Secondary Structure
MKQIVLDYSCLFDDDIIIDDIKIYHYLVNVRRLRVGDRLNILLGKREIRFSEILSIENHR
CCEEEEEEEEEECCCEEEEHHHHHHHHHHHEEEECCCEEEEEEECCCCCHHHHHCCCCCE
IRFATLRVEELKMRDFAIDIFISNLKGRKLDLSLRQVVEIGVNSINIVNADNSIAKLDMD
EEEEEEEEEHHHHEEEEEEEEEECCCCCEECHHHHHHHHHCCCEEEEEECCCCEEEEECC
NLEPKKLRFLKIIDEALKQSGNAQIPSINFYNDFFSIPYSSSVNYYVAYSKGDFLHLGEN
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHCCCCCCCCCCEEEEECCCCEEEECCC
ISVFGKIGILIGPEGCFLKLEIDFFKKLNFKFVRFNTPILRADTAIVYALAHFKLLLEGN
EEEEEEEEEEECCCCCEEEEEEEHHHCCCEEEEEECCCEEECCHHHHEEEEEEEEEEECC
NG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]