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The map label for this gene is ppnK [H]

Identifier: 203284232

GI number: 203284232

Start: 333823

End: 334722

Strand: Reverse

Name: ppnK [H]

Synonym: BDU_314

Alternate gene names: 203284232

Gene position: 334722-333823 (Counterclockwise)

Preceding gene: 203284233

Following gene: 203284231

Centisome position: 35.93

GC content: 27.0

Gene sequence:

>900_bases
TTGATATTGAACGAATATTTGATAAGGGCGAGTTTGAAAAGATTCCATATAAGGAGAATAATGAGTAGTAAAGTTTTAAT
TTACGTAAATTATTCAAATTTAGATGCTGAAGTTCTTGCTTGTGAGATACAAAGATATTTGGAGTTTAAGTATGGTGTTT
TAAGTTTATTTGCAGGAATGAATAAATCTTTATGTGATATATCAAATAAAGATAATTTGATTTTTGCAATAACTTTGGGT
GGAGATGGTACTGTTTTATTAGCCAGTAGTTTACTTTTAAAGAATGATATTGATATTCCTATTATTTCAATCAATTTGGG
TAAAGTAGGATTTTTGGCAGATATAAAGCCTAGAGACTTTAAAGATGTGATAGATAAGTTTTTTAATAATTCTTTAGTGA
TTCATAAAAAATATTTACTTTGTATTAGTGCTTATGAAGATGGAAATAATTTATTTACTAAATATGCTTTAAATGATGTG
ATTATTCGTTCTAGTATTTTAAATAAATTGATTTATGTAAATCTTAAAGTTAATTCAGAAGATTTTCTTTCGTATAAGAG
TGATGGAATAATATTTGCAACTCCAACAGGATCAACTGGGTATTCTTTTTCAGCGGGAGGAGCTATTTTAGAATCTGATC
TTAAGGCTTTCATTTTAACACCCATTTCACCGCATTCTGTTTATAATCGTTCTTTTATTTTTTCAAGTAAAAGTAAACTG
TCACTTGCGTTTCAAAAAGGGTATACATTAAATTCTGCATCAATTTTTGTTGATGGTGTTAATATTGGTACATTTGGGGT
TAATATTGTTTTTGAGTTAAAACTTGATAATAAAAGTTTACGTTTTGCATCCTTTTGTACGGATACTTTTGTTAGAAGAC
TGAAAAATAAGTTATTATAG

Upstream 100 bases:

>100_bases
GAGCCTCCTGCAACTTTACAAACTTTTGATAAAAAATATATATCTGGTGTTGTTAAGATTGAGAAAGAAGAAAATTTTGA
GAGTGAATATTTAATTTTAA

Downstream 100 bases:

>100_bases
ACTATGGTTTTATAATAATTTATTTTATGTGTCATATATTGATTGAACTTTTTATAAAAAATTTGATTTTAATTAAAAAG
TTTTATATGAGGCTAAATGT

Product: probable inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MILNEYLIRASLKRFHIRRIMSSKVLIYVNYSNLDAEVLACEIQRYLEFKYGVLSLFAGMNKSLCDISNKDNLIFAITLG
GDGTVLLASSLLLKNDIDIPIISINLGKVGFLADIKPRDFKDVIDKFFNNSLVIHKKYLLCISAYEDGNNLFTKYALNDV
IIRSSILNKLIYVNLKVNSEDFLSYKSDGIIFATPTGSTGYSFSAGGAILESDLKAFILTPISPHSVYNRSFIFSSKSKL
SLAFQKGYTLNSASIFVDGVNIGTFGVNIVFELKLDNKSLRFASFCTDTFVRRLKNKLL

Sequences:

>Translated_299_residues
MILNEYLIRASLKRFHIRRIMSSKVLIYVNYSNLDAEVLACEIQRYLEFKYGVLSLFAGMNKSLCDISNKDNLIFAITLG
GDGTVLLASSLLLKNDIDIPIISINLGKVGFLADIKPRDFKDVIDKFFNNSLVIHKKYLLCISAYEDGNNLFTKYALNDV
IIRSSILNKLIYVNLKVNSEDFLSYKSDGIIFATPTGSTGYSFSAGGAILESDLKAFILTPISPHSVYNRSFIFSSKSKL
SLAFQKGYTLNSASIFVDGVNIGTFGVNIVFELKLDNKSLRFASFCTDTFVRRLKNKLL
>Mature_299_residues
MILNEYLIRASLKRFHIRRIMSSKVLIYVNYSNLDAEVLACEIQRYLEFKYGVLSLFAGMNKSLCDISNKDNLIFAITLG
GDGTVLLASSLLLKNDIDIPIISINLGKVGFLADIKPRDFKDVIDKFFNNSLVIHKKYLLCISAYEDGNNLFTKYALNDV
IIRSSILNKLIYVNLKVNSEDFLSYKSDGIIFATPTGSTGYSFSAGGAILESDLKAFILTPISPHSVYNRSFIFSSKSKL
SLAFQKGYTLNSASIFVDGVNIGTFGVNIVFELKLDNKSLRFASFCTDTFVRRLKNKLL

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=170, Percent_Identity=32.9411764705882, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6325068, Length=212, Percent_Identity=30.188679245283, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320794, Length=181, Percent_Identity=32.5966850828729, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6322509, Length=175, Percent_Identity=33.1428571428571, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 33471; Mature: 33471

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILNEYLIRASLKRFHIRRIMSSKVLIYVNYSNLDAEVLACEIQRYLEFKYGVLSLFAGM
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC
NKSLCDISNKDNLIFAITLGGDGTVLLASSLLLKNDIDIPIISINLGKVGFLADIKPRDF
CCCCCCCCCCCCEEEEEEECCCCEEEEEEHHHHCCCCCCEEEEEECCCEEEEECCCCCCH
KDVIDKFFNNSLVIHKKYLLCISAYEDGNNLFTKYALNDVIIRSSILNKLIYVNLKVNSE
HHHHHHHHCCCEEEEEEEEEEEEEEECCCCEEEEHHHHHHHHHHHHHCEEEEEEEEECCH
DFLSYKSDGIIFATPTGSTGYSFSAGGAILESDLKAFILTPISPHSVYNRSFIFSSKSKL
HHHEECCCCEEEECCCCCCCCEECCCCCEECCCCEEEEECCCCCCHHCCCEEEEECCCCE
SLAFQKGYTLNSASIFVDGVNIGTFGVNIVFELKLDNKSLRFASFCTDTFVRRLKNKLL
EEEEECCEEECCCEEEEEEEEEEEEEEEEEEEEEECCCCEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILNEYLIRASLKRFHIRRIMSSKVLIYVNYSNLDAEVLACEIQRYLEFKYGVLSLFAGM
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCC
NKSLCDISNKDNLIFAITLGGDGTVLLASSLLLKNDIDIPIISINLGKVGFLADIKPRDF
CCCCCCCCCCCCEEEEEEECCCCEEEEEEHHHHCCCCCCEEEEEECCCEEEEECCCCCCH
KDVIDKFFNNSLVIHKKYLLCISAYEDGNNLFTKYALNDVIIRSSILNKLIYVNLKVNSE
HHHHHHHHCCCEEEEEEEEEEEEEEECCCCEEEEHHHHHHHHHHHHHCEEEEEEEEECCH
DFLSYKSDGIIFATPTGSTGYSFSAGGAILESDLKAFILTPISPHSVYNRSFIFSSKSKL
HHHEECCCCEEEECCCCCCCCEECCCCCEECCCCEEEEECCCCCCHHCCCEEEEECCCCE
SLAFQKGYTLNSASIFVDGVNIGTFGVNIVFELKLDNKSLRFASFCTDTFVRRLKNKLL
EEEEECCEEECCCEEEEEEEEEEEEEEEEEEEEEECCCCEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]