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The map label for this gene is hslV

Identifier: 203284217

GI number: 203284217

Start: 319293

End: 319835

Strand: Reverse

Name: hslV

Synonym: BDU_299

Alternate gene names: 203284217

Gene position: 319835-319293 (Counterclockwise)

Preceding gene: 203284218

Following gene: 203284216

Centisome position: 34.33

GC content: 34.99

Gene sequence:

>543_bases
GTGAATTTTAAAGGAACCACAGTTATTGCAATAAGAAGGGCAGGAAAGACTGTAGTAGCAGCAGATGGGCAGGTGACTTT
TGGATATACTGTTTTAAAATCTAATGCTGTAAAAATAAGAAAGTTGGTTAATGGAAAGATTTTGGCAGGATTTGCAGGTT
CGACTTCTGATGCTATTACTCTTTTTGAGAAATTTGAGGAAAAGGTGAAGTCTAGAGAAGATGGAATTATTGATATTAAG
AGAGCCGCTGTGGATCTTGCAAAAGATTGGAGATCTGATAAAATACTGCATAAACTTGAGGCAATGATGCTTGTTGCTGA
TTCTGATAATATTTTATTAATTTCAGGTACTGGAGATGTTGTTGAGCCTGAAGAGGATGTAATTTCCATTGGTAGTGGTG
GAAATTATGCATACTCAGCAGCTCTTGCTTACATGGAAAATAAAAAATTAAGTGCTGCTGATATTGCTTTTAAAGCTTTA
AAGATAGCAGCAAGAGTTTGTATATATACAAATTCAAATATTGTGCTTGAGGAGATTGGTTGA

Upstream 100 bases:

>100_bases
AATTGTTGATGATATGGATAGTTGTTGTATAGGTGGAGATATATCTAGTATGCTTATTAGTGAGTTATTGAATGAAATAT
CTAAATAGGGAGGGGACGTC

Downstream 100 bases:

>100_bases
TGAATAAGATTGAGGATCAAAGTATAGTACCTAGAGAAATTGTTGCAGAATTGGATAAGTATATAATAGGACAGTCAGAA
GCAAAGAAATTGGTTTCAAT

Product: ATP-dependent protease peptidase subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 180; Mature: 180

Protein sequence:

>180_residues
MNFKGTTVIAIRRAGKTVVAADGQVTFGYTVLKSNAVKIRKLVNGKILAGFAGSTSDAITLFEKFEEKVKSREDGIIDIK
RAAVDLAKDWRSDKILHKLEAMMLVADSDNILLISGTGDVVEPEEDVISIGSGGNYAYSAALAYMENKKLSAADIAFKAL
KIAARVCIYTNSNIVLEEIG

Sequences:

>Translated_180_residues
MNFKGTTVIAIRRAGKTVVAADGQVTFGYTVLKSNAVKIRKLVNGKILAGFAGSTSDAITLFEKFEEKVKSREDGIIDIK
RAAVDLAKDWRSDKILHKLEAMMLVADSDNILLISGTGDVVEPEEDVISIGSGGNYAYSAALAYMENKKLSAADIAFKAL
KIAARVCIYTNSNIVLEEIG
>Mature_180_residues
MNFKGTTVIAIRRAGKTVVAADGQVTFGYTVLKSNAVKIRKLVNGKILAGFAGSTSDAITLFEKFEEKVKSREDGIIDIK
RAAVDLAKDWRSDKILHKLEAMMLVADSDNILLISGTGDVVEPEEDVISIGSGGNYAYSAALAYMENKKLSAADIAFKAL
KIAARVCIYTNSNIVLEEIG

Specific function: Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery

COG id: COG5405

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), peptidase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase T1B family. HslV subfamily

Homologues:

Organism=Escherichia coli, GI1790367, Length=174, Percent_Identity=54.0229885057471, Blast_Score=193, Evalue=5e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLV_BORDL (B5RLC2)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002221957.1
- EnsemblBacteria:   EBBORT00000001412
- GeneID:   6918383
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_299
- GeneTree:   EBGT00050000007567
- HOGENOM:   HBG288822
- OMA:   AADICVY
- ProtClustDB:   PRK05456
- HAMAP:   MF_00248
- InterPro:   IPR022281
- InterPro:   IPR001353
- TIGRFAMs:   TIGR03692

Pfam domain/function: PF00227 Proteasome

EC number: 3.4.25.-

Molecular weight: Translated: 19377; Mature: 19377

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: NA

Important sites: ACT_SITE 6-6

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKGTTVIAIRRAGKTVVAADGQVTFGYTVLKSNAVKIRKLVNGKILAGFAGSTSDAIT
CCCCCEEEEEEEECCCEEEEECCEEEEEEEEEECCHHEEEHHHCCEEEEEECCCCCHHHH
LFEKFEEKVKSREDGIIDIKRAAVDLAKDWRSDKILHKLEAMMLVADSDNILLISGTGDV
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHEEEEEECCCCEEEEECCCCC
VEPEEDVISIGSGGNYAYSAALAYMENKKLSAADIAFKALKIAARVCIYTNSNIVLEEIG
CCCCHHEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEEECCCEEEEECC
>Mature Secondary Structure
MNFKGTTVIAIRRAGKTVVAADGQVTFGYTVLKSNAVKIRKLVNGKILAGFAGSTSDAIT
CCCCCEEEEEEEECCCEEEEECCEEEEEEEEEECCHHEEEHHHCCEEEEEECCCCCHHHH
LFEKFEEKVKSREDGIIDIKRAAVDLAKDWRSDKILHKLEAMMLVADSDNILLISGTGDV
HHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHEEEEEECCCCEEEEECCCCC
VEPEEDVISIGSGGNYAYSAALAYMENKKLSAADIAFKALKIAARVCIYTNSNIVLEEIG
CCCCHHEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA