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The map label for this gene is engD [H]

Identifier: 203284154

GI number: 203284154

Start: 250020

End: 251126

Strand: Reverse

Name: engD [H]

Synonym: BDU_235

Alternate gene names: 203284154

Gene position: 251126-250020 (Counterclockwise)

Preceding gene: 203284155

Following gene: 203284153

Centisome position: 26.95

GC content: 31.26

Gene sequence:

>1107_bases
ATGGCACTTAATGTGGGAATAGTGGGATTGCCCAATGTTGGGAAATCTACCTTATTTTCGTCCTTAACAGCATCAAAGTC
AGAGATTGCTAATTATCCTTTTTGTACTATTGATCCTAATGTGGGAATAGTAGAAGTTCCTGATGCGAGACTTTTGAAAA
TTGCTAATTTAGTATCGTCAAAAAAAATAATTCCTGCTGTGATAGAATTTGTTGATATTGCGGGTCTTGTTAGAGGGGCT
TCCAAAGGAGAAGGACTTGGTAATAAATTTTTAGCGAATATTCGTGAAGTTTCTATTATTGTTCATGTTGTTAGATGCTT
TAATGATAGAGAAGTTATTCATGTTGATGGGGATATAGATCCTCAAAGGGATATAAGTACAATTAATACAGAATTATGTC
TTGCAGATCTTGAAACGGTTCAAAAAAGTATTTTAAAAAATGAAAAAAATGCAAAGAGTATTGATAAAAATATTAGTGAA
AATGCAAAGATAATTGTTTCACTTCTTCGAGATCTTGAAAAACATTTGATTGATGTTAAACCAGCAAGCGAGTTTAAATT
TGATGAATTTGGTTATACTTTTGTTAAGTCTTTAAATCTTTTGACTATGAAAAATGTTATATATGTTTGCAATGTTGATG
AGGATTCACTTTCTGGTAATAAATATACTGATGTTGTTAAAAATATAGCTTTAAGTGAGGGTAATAATTATTTAATTTTG
TGTGCTAAGATTGAGGCAGAACTTGCTGAAATTCAAGACTTAAGCGAGAGAAGAGAAATGTTAGAATCTATGGGTATCAA
AGAGAGTGGTCTTAGTAATTTAATACGTACTTCTTATTATACTTTAGGTTTAAGAACCTATTTTACTGCAGGAGAACAAG
AAGTTAGAGCTTGGACTTTTATTGATGGAATGAAAGCCCCAGAAGCTGCAGGTATTATTCATAGTGATTTTCAGAGAGGT
TTTATTAAAGCAGAGGTGTATTCATTTGATGATTTGATTGAGTTTCAAGGCGTTCATGGTTTAAAGGAAAAGGGACGTCT
TAGACTTGAGGGAAAAGATTATTTGGTAAGAGATGGTGATATAATTTTATTTAAATTTAATGTTTAA

Upstream 100 bases:

>100_bases
ATATTATAATTTATCTGTTGAAGTAAATTTCAATCAACAAATAGGTAGAGGAGAATTTGCATATTTAATTAATTAAATTT
TACTTTATGGAGTTTTATTT

Downstream 100 bases:

>100_bases
ATAAATTAAGATGCAGGAATTATTTTTTTATAAAATCAGATTACTTTTTATTTTTTGTCTTAATATAAGCTTTTCTATAT
ATTATATGTTGTTATATAGG

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MALNVGIVGLPNVGKSTLFSSLTASKSEIANYPFCTIDPNVGIVEVPDARLLKIANLVSSKKIIPAVIEFVDIAGLVRGA
SKGEGLGNKFLANIREVSIIVHVVRCFNDREVIHVDGDIDPQRDISTINTELCLADLETVQKSILKNEKNAKSIDKNISE
NAKIIVSLLRDLEKHLIDVKPASEFKFDEFGYTFVKSLNLLTMKNVIYVCNVDEDSLSGNKYTDVVKNIALSEGNNYLIL
CAKIEAELAEIQDLSERREMLESMGIKESGLSNLIRTSYYTLGLRTYFTAGEQEVRAWTFIDGMKAPEAAGIIHSDFQRG
FIKAEVYSFDDLIEFQGVHGLKEKGRLRLEGKDYLVRDGDIILFKFNV

Sequences:

>Translated_368_residues
MALNVGIVGLPNVGKSTLFSSLTASKSEIANYPFCTIDPNVGIVEVPDARLLKIANLVSSKKIIPAVIEFVDIAGLVRGA
SKGEGLGNKFLANIREVSIIVHVVRCFNDREVIHVDGDIDPQRDISTINTELCLADLETVQKSILKNEKNAKSIDKNISE
NAKIIVSLLRDLEKHLIDVKPASEFKFDEFGYTFVKSLNLLTMKNVIYVCNVDEDSLSGNKYTDVVKNIALSEGNNYLIL
CAKIEAELAEIQDLSERREMLESMGIKESGLSNLIRTSYYTLGLRTYFTAGEQEVRAWTFIDGMKAPEAAGIIHSDFQRG
FIKAEVYSFDDLIEFQGVHGLKEKGRLRLEGKDYLVRDGDIILFKFNV
>Mature_367_residues
ALNVGIVGLPNVGKSTLFSSLTASKSEIANYPFCTIDPNVGIVEVPDARLLKIANLVSSKKIIPAVIEFVDIAGLVRGAS
KGEGLGNKFLANIREVSIIVHVVRCFNDREVIHVDGDIDPQRDISTINTELCLADLETVQKSILKNEKNAKSIDKNISEN
AKIIVSLLRDLEKHLIDVKPASEFKFDEFGYTFVKSLNLLTMKNVIYVCNVDEDSLSGNKYTDVVKNIALSEGNNYLILC
AKIEAELAEIQDLSERREMLESMGIKESGLSNLIRTSYYTLGLRTYFTAGEQEVRAWTFIDGMKAPEAAGIIHSDFQRGF
IKAEVYSFDDLIEFQGVHGLKEKGRLRLEGKDYLVRDGDIILFKFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=375, Percent_Identity=40.5333333333333, Blast_Score=254, Evalue=7e-68,
Organism=Homo sapiens, GI58761502, Length=173, Percent_Identity=39.3063583815029, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI24308117, Length=113, Percent_Identity=31.858407079646, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1787454, Length=370, Percent_Identity=49.1891891891892, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1789574, Length=177, Percent_Identity=29.3785310734463, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17509631, Length=371, Percent_Identity=39.622641509434, Blast_Score=249, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17552324, Length=185, Percent_Identity=28.6486486486486, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319499, Length=372, Percent_Identity=39.247311827957, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6321773, Length=380, Percent_Identity=38.1578947368421, Blast_Score=225, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6321649, Length=165, Percent_Identity=35.7575757575758, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24640873, Length=374, Percent_Identity=40.6417112299465, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24640877, Length=374, Percent_Identity=40.6417112299465, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24640875, Length=374, Percent_Identity=40.6417112299465, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI24640879, Length=333, Percent_Identity=38.4384384384384, Blast_Score=197, Evalue=7e-51,
Organism=Drosophila melanogaster, GI24585318, Length=191, Percent_Identity=32.4607329842932, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40935; Mature: 40803

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALNVGIVGLPNVGKSTLFSSLTASKSEIANYPFCTIDPNVGIVEVPDARLLKIANLVSS
CEEEEEEEECCCCCHHHHHHHHHCCHHHHCCCCEEEECCCCCEEECCCHHHHHHHHHHHC
KKIIPAVIEFVDIAGLVRGASKGEGLGNKFLANIREVSIIVHVVRCFNDREVIHVDGDID
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
PQRDISTINTELCLADLETVQKSILKNEKNAKSIDKNISENAKIIVSLLRDLEKHLIDVK
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
PASEFKFDEFGYTFVKSLNLLTMKNVIYVCNVDEDSLSGNKYTDVVKNIALSEGNNYLIL
CCCCCCCHHHHHHHHHHHHHEEECCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEE
CAKIEAELAEIQDLSERREMLESMGIKESGLSNLIRTSYYTLGLRTYFTAGEQEVRAWTF
EEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEHHEEECCCCHHHEEEEE
IDGMKAPEAAGIIHSDFQRGFIKAEVYSFDDLIEFQGVHGLKEKGRLRLEGKDYLVRDGD
ECCCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHCCCCCCCCCCEEEECCCCEEEECCC
IILFKFNV
EEEEEECC
>Mature Secondary Structure 
ALNVGIVGLPNVGKSTLFSSLTASKSEIANYPFCTIDPNVGIVEVPDARLLKIANLVSS
EEEEEEEECCCCCHHHHHHHHHCCHHHHCCCCEEEECCCCCEEECCCHHHHHHHHHHHC
KKIIPAVIEFVDIAGLVRGASKGEGLGNKFLANIREVSIIVHVVRCFNDREVIHVDGDID
CCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
PQRDISTINTELCLADLETVQKSILKNEKNAKSIDKNISENAKIIVSLLRDLEKHLIDVK
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
PASEFKFDEFGYTFVKSLNLLTMKNVIYVCNVDEDSLSGNKYTDVVKNIALSEGNNYLIL
CCCCCCCHHHHHHHHHHHHHEEECCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEE
CAKIEAELAEIQDLSERREMLESMGIKESGLSNLIRTSYYTLGLRTYFTAGEQEVRAWTF
EEECHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEHHEEECCCCHHHEEEEE
IDGMKAPEAAGIIHSDFQRGFIKAEVYSFDDLIEFQGVHGLKEKGRLRLEGKDYLVRDGD
ECCCCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHCCCCCCCCCCEEEECCCCEEEECCC
IILFKFNV
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]