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The map label for this gene is murE [H]

Identifier: 203284121

GI number: 203284121

Start: 211143

End: 212669

Strand: Reverse

Name: murE [H]

Synonym: BDU_199

Alternate gene names: 203284121

Gene position: 212669-211143 (Counterclockwise)

Preceding gene: 203284126

Following gene: 203284120

Centisome position: 22.83

GC content: 29.6

Gene sequence:

>1527_bases
ATGAATATGAATAGAAAAGTACTTAATGATGTTTTATTTAGATTAAATCAAAATTTGATACAGAAAATTATAGGGTCTTG
TGAAGTGGAAATATTGGGACTTGCATATGATTCAAGATGTGTTTCATTTAATTTCGTTTTTTTTGCTCTTCCTGGGCTGC
ATTTTAATGGTCAAAAATTTATTGAATCAGCAATTCAACGAGGTAGTAATGTTATTGTTCATACTAATGATATTGATTTT
TATGATTCCAACGTAACGTATATTAAAGTTGATCCTTGTAATATAAAAAGATTTATGTCAAATTTTGCTCATGTTTTTTA
TGATGAGCCCTCAAAAAAGCTTAAAATTATTGGTGTTACAGGAACTGATGGTAAGAGTTCTGTTTGTTTTTATATTTATA
CTTTATTAAAATCTATGGGTGCTAAAGTTGGGTTTATCTCAACAGTGTTTCTTGAAGATGGAAGTGGTACTTTAGTGAAA
AATCCCTATAGGCAGTCTACTCCAGAATCAACAGAAATTCATTTAGTTCTCAGTAAAATGGTTGCTAATAATGTTGAATA
TGCTATTATTGAATCAACTTCTCATGGTCTTGATGATAGGACATCAAGGCTTCTTGATATTGAATATTCTGTTGCTGTTT
TGACTAATATCAGTCATGAACATCTTGAATTTCATGGTACGATGCAGAATTATTTGAGAGCCAAGCTTAATCTTTTTTAT
TCTGCAAATTTTCATGGTGGTTTTGGTATTATCAATATGGATGATAATCATTTTTCTGTGTTTATGAATTCTATTAATAG
GACTTATACCTATAGTTTAAAAAATGAAAAGGCTGATTTTTTTATAAGTAAGATTAATGAACAAATGGGTTTTACTGAAT
TTGAGTTTTATCATAATAAAATTAAATATGCTGCTAGAGTTAATTTGACGGGTAGTTTTAATGTTGAAAATGTTATGGCT
GCCTTAATTGTTGTTAGTCAAATTATGAATGTTGATATTTCAAAACTTATTTGTAAGCTTATTAATATTGAGAGTCTTTA
TGGACGCATGCAAGATATTAATTTTGGCCAAGATTTTTCTTTAATTGTTGATTATGCACATACCCCAGGTGCTTTTCTTA
AAATGTTTCCTATATTTAAACGACTTGCAAAGAAGCGTTTAATTTCTGTTTTTGGTTCTGCTGGGGAGAGAGATATTCTT
AAGAGAAGGTTGCAGGGAGAAATTGCAGATGAGTATTCAGATGTAATAATACTTTGTGATGAGGATCCAAGAGGTGAGGA
TTCTATGCAAATAATTAGAGATATTGCGGAAGGAATTGTGACAAAAACCTTAAATAAAGATTTGTTTTTTATTCCTGATA
GAAAATGTGCAATTGAAAAAGCAATAAATATTGCATGTAGTGACGATTTGGTGGTTACTCTTGGAAAGGGACATGAAAGT
TCAATAATCTATAAAGATAAAAGTATTTTTTGGGATGAACAAGCGGTTATTAAAGATATTATTTTAAGTTTGGGAAATAG
GGGTTAG

Upstream 100 bases:

>100_bases
ACAGTCTTTTTTCACTTTTATAGTTTAAATAATAATTGATTTTTTGGGTATTCTCTTTTGAAATTTTTATATAAAAATAA
ATTTTATTTTTGTAAACTTT

Downstream 100 bases:

>100_bases
TGTTATGAAAAAAAATCTTATGTTAATATTTGGAGGAGTTTCTTTTGAACATGAAATTTCTCTTAGATCTGCTTATGGGA
TTTATTCATCTCTTTTGAAA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate

Products: ADP; phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-; $heptanedioate

Alternate protein names: UDP-MurNAc-tripeptide synthetase [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MNMNRKVLNDVLFRLNQNLIQKIIGSCEVEILGLAYDSRCVSFNFVFFALPGLHFNGQKFIESAIQRGSNVIVHTNDIDF
YDSNVTYIKVDPCNIKRFMSNFAHVFYDEPSKKLKIIGVTGTDGKSSVCFYIYTLLKSMGAKVGFISTVFLEDGSGTLVK
NPYRQSTPESTEIHLVLSKMVANNVEYAIIESTSHGLDDRTSRLLDIEYSVAVLTNISHEHLEFHGTMQNYLRAKLNLFY
SANFHGGFGIINMDDNHFSVFMNSINRTYTYSLKNEKADFFISKINEQMGFTEFEFYHNKIKYAARVNLTGSFNVENVMA
ALIVVSQIMNVDISKLICKLINIESLYGRMQDINFGQDFSLIVDYAHTPGAFLKMFPIFKRLAKKRLISVFGSAGERDIL
KRRLQGEIADEYSDVIILCDEDPRGEDSMQIIRDIAEGIVTKTLNKDLFFIPDRKCAIEKAINIACSDDLVVTLGKGHES
SIIYKDKSIFWDEQAVIKDIILSLGNRG

Sequences:

>Translated_508_residues
MNMNRKVLNDVLFRLNQNLIQKIIGSCEVEILGLAYDSRCVSFNFVFFALPGLHFNGQKFIESAIQRGSNVIVHTNDIDF
YDSNVTYIKVDPCNIKRFMSNFAHVFYDEPSKKLKIIGVTGTDGKSSVCFYIYTLLKSMGAKVGFISTVFLEDGSGTLVK
NPYRQSTPESTEIHLVLSKMVANNVEYAIIESTSHGLDDRTSRLLDIEYSVAVLTNISHEHLEFHGTMQNYLRAKLNLFY
SANFHGGFGIINMDDNHFSVFMNSINRTYTYSLKNEKADFFISKINEQMGFTEFEFYHNKIKYAARVNLTGSFNVENVMA
ALIVVSQIMNVDISKLICKLINIESLYGRMQDINFGQDFSLIVDYAHTPGAFLKMFPIFKRLAKKRLISVFGSAGERDIL
KRRLQGEIADEYSDVIILCDEDPRGEDSMQIIRDIAEGIVTKTLNKDLFFIPDRKCAIEKAINIACSDDLVVTLGKGHES
SIIYKDKSIFWDEQAVIKDIILSLGNRG
>Mature_508_residues
MNMNRKVLNDVLFRLNQNLIQKIIGSCEVEILGLAYDSRCVSFNFVFFALPGLHFNGQKFIESAIQRGSNVIVHTNDIDF
YDSNVTYIKVDPCNIKRFMSNFAHVFYDEPSKKLKIIGVTGTDGKSSVCFYIYTLLKSMGAKVGFISTVFLEDGSGTLVK
NPYRQSTPESTEIHLVLSKMVANNVEYAIIESTSHGLDDRTSRLLDIEYSVAVLTNISHEHLEFHGTMQNYLRAKLNLFY
SANFHGGFGIINMDDNHFSVFMNSINRTYTYSLKNEKADFFISKINEQMGFTEFEFYHNKIKYAARVNLTGSFNVENVMA
ALIVVSQIMNVDISKLICKLINIESLYGRMQDINFGQDFSLIVDYAHTPGAFLKMFPIFKRLAKKRLISVFGSAGERDIL
KRRLQGEIADEYSDVIILCDEDPRGEDSMQIIRDIAEGIVTKTLNKDLFFIPDRKCAIEKAINIACSDDLVVTLGKGHES
SIIYKDKSIFWDEQAVIKDIILSLGNRG

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=487, Percent_Identity=27.3100616016427, Blast_Score=165, Evalue=7e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.13

Molecular weight: Translated: 57629; Mature: 57629

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMNRKVLNDVLFRLNQNLIQKIIGSCEVEILGLAYDSRCVSFNFVFFALPGLHFNGQKF
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEEEEEECCCCCCHHHH
IESAIQRGSNVIVHTNDIDFYDSNVTYIKVDPCNIKRFMSNFAHVFYDEPSKKLKIIGVT
HHHHHHCCCEEEEECCCCEEECCCEEEEEECCCHHHHHHHHHHHEEECCCCCEEEEEEEE
GTDGKSSVCFYIYTLLKSMGAKVGFISTVFLEDGSGTLVKNPYRQSTPESTEIHLVLSKM
CCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCEEECCCCCCCCCCCEEHHHHHHH
VANNVEYAIIESTSHGLDDRTSRLLDIEYSVAVLTNISHEHLEFHGTMQNYLRAKLNLFY
HHCCCCEEEEECCCCCCCCHHHEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHEEEEE
SANFHGGFGIINMDDNHFSVFMNSINRTYTYSLKNEKADFFISKINEQMGFTEFEFYHNK
EECCCCCEEEEEECCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHH
IKYAARVNLTGSFNVENVMAALIVVSQIMNVDISKLICKLINIESLYGRMQDINFGQDFS
EEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHCCCCCCCCE
LIVDYAHTPGAFLKMFPIFKRLAKKRLISVFGSAGERDILKRRLQGEIADEYSDVIILCD
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEC
EDPRGEDSMQIIRDIAEGIVTKTLNKDLFFIPDRKCAIEKAINIACSDDLVVTLGKGHES
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCEEEECCCEEEEECCCCCC
SIIYKDKSIFWDEQAVIKDIILSLGNRG
CEEEECCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNMNRKVLNDVLFRLNQNLIQKIIGSCEVEILGLAYDSRCVSFNFVFFALPGLHFNGQKF
CCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEEEEEEECCCCCCHHHH
IESAIQRGSNVIVHTNDIDFYDSNVTYIKVDPCNIKRFMSNFAHVFYDEPSKKLKIIGVT
HHHHHHCCCEEEEECCCCEEECCCEEEEEECCCHHHHHHHHHHHEEECCCCCEEEEEEEE
GTDGKSSVCFYIYTLLKSMGAKVGFISTVFLEDGSGTLVKNPYRQSTPESTEIHLVLSKM
CCCCCCHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCEEECCCCCCCCCCCEEHHHHHHH
VANNVEYAIIESTSHGLDDRTSRLLDIEYSVAVLTNISHEHLEFHGTMQNYLRAKLNLFY
HHCCCCEEEEECCCCCCCCHHHEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHEEEEE
SANFHGGFGIINMDDNHFSVFMNSINRTYTYSLKNEKADFFISKINEQMGFTEFEFYHNK
EECCCCCEEEEEECCCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHH
IKYAARVNLTGSFNVENVMAALIVVSQIMNVDISKLICKLINIESLYGRMQDINFGQDFS
EEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHCCCCCCCCE
LIVDYAHTPGAFLKMFPIFKRLAKKRLISVFGSAGERDILKRRLQGEIADEYSDVIILCD
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEC
EDPRGEDSMQIIRDIAEGIVTKTLNKDLFFIPDRKCAIEKAINIACSDDLVVTLGKGHES
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHCEEEECCCEEEEECCCCCC
SIIYKDKSIFWDEQAVIKDIILSLGNRG
CEEEECCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 1.5 {meso-lanthionine}} 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.

Substrates: ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate; meso-2,6-diaminoheptanedioate

Specific reaction: ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N- $acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino- $heptanedioate

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]