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The map label for this gene is ddlA

Identifier: 203284120

GI number: 203284120

Start: 210044

End: 211138

Strand: Reverse

Name: ddlA

Synonym: BDU_198

Alternate gene names: 203284120

Gene position: 211138-210044 (Counterclockwise)

Preceding gene: 203284121

Following gene: 203284115

Centisome position: 22.66

GC content: 27.85

Gene sequence:

>1095_bases
ATGAAAAAAAATCTTATGTTAATATTTGGAGGAGTTTCTTTTGAACATGAAATTTCTCTTAGATCTGCTTATGGGATTTA
TTCATCTCTTTTGAAACTTGATAAGTATAATGTATTTTCGGTTTTTGTTGATAAAGTTACTGGAATTTGGTATTTGTTAG
ATTCTGTTCCCAGTAGCGCTGAATTGATTAAAAGACATACTACTTCTATTGTTAATTTTATTCCTGGTTGTGGGATATTT
GTAAATAATAAATCTCTTGAGATAGATGTTATATTTCCTATTATTCATGGAAGAACAGGTGAGGATGGTGCTATTCAGGG
ATTTGTAAAGATGATGGATATTCCTTGTGTTGGTGCTGGTATTTTAGGAAGTGCTATTTCTATTAATAAATATTTCTGTA
AGGTTTTGCTTAAAAGTTTTAATATTCCTGTAGTGTCTTTTATTGGATTTAAAAAGGATGATTATATTTTAAATAAAGAG
GGAATAAAAGAGGATATAAATAATAAATTAAATTATCCTGTAATTGTGAAGCCTTCTGTATTAGGTTCTTCAATTGGAAT
TAATGTTGCATATAATGTTTCTCAGATTGAAAAATATATTGAAGAAGCTTTTGAATATGATTTGACAGTTGTTGTAGAAA
AATTTATTAAAGCAAGAGAAATTGAATGTGCTGTTATTGGAAATGATCAGATTAAAATATTTACACCCGGTGAAATTGTT
GTGCAGGATTTTATATTTTATGATTATGATGCTAAGTATTCTACTGTTCCTGGAGATTCAATTGTGTTTAATATCCCTGC
TCATCTTGATATGAAACATTTATTAGATATTAAAGAATATGCATTTTTAACTTATAAATATTTGGAACTTAGAGGCATGG
CAAGGATTGATTTTTTGATATCAAAAGATACCAATTTACTCTATGTTAATGAAGTAAATACAATTCCAGGTTTTACAGAT
ATTTCTATGTTTGCTAAAATGTGTGAACATGATGGTCTTAGTTATGAGAGTTTGGTTGATAAATTAATCACTTTGGCTTT
TGAGAGTTATAAAAAGCGTAAGGATAAAATTGATTTTACTAGGTTAGAGAGTTAG

Upstream 100 bases:

>100_bases
CATGAAAGTTCAATAATCTATAAAGATAAAAGTATTTTTTGGGATGAACAAGCGGTTATTAAAGATATTATTTTAAGTTT
GGGAAATAGGGGTTAGTGTT

Downstream 100 bases:

>100_bases
TTTAAATTTAAATTTTTTAATTTTTCAATTTCTTTATCATTTTGAAGTTTAAAAAAGGGGTCTAAATCTTGTTTTGATGA
TTTTGGAAGTATTCCTGAAT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MKKNLMLIFGGVSFEHEISLRSAYGIYSSLLKLDKYNVFSVFVDKVTGIWYLLDSVPSSAELIKRHTTSIVNFIPGCGIF
VNNKSLEIDVIFPIIHGRTGEDGAIQGFVKMMDIPCVGAGILGSAISINKYFCKVLLKSFNIPVVSFIGFKKDDYILNKE
GIKEDINNKLNYPVIVKPSVLGSSIGINVAYNVSQIEKYIEEAFEYDLTVVVEKFIKAREIECAVIGNDQIKIFTPGEIV
VQDFIFYDYDAKYSTVPGDSIVFNIPAHLDMKHLLDIKEYAFLTYKYLELRGMARIDFLISKDTNLLYVNEVNTIPGFTD
ISMFAKMCEHDGLSYESLVDKLITLAFESYKKRKDKIDFTRLES

Sequences:

>Translated_364_residues
MKKNLMLIFGGVSFEHEISLRSAYGIYSSLLKLDKYNVFSVFVDKVTGIWYLLDSVPSSAELIKRHTTSIVNFIPGCGIF
VNNKSLEIDVIFPIIHGRTGEDGAIQGFVKMMDIPCVGAGILGSAISINKYFCKVLLKSFNIPVVSFIGFKKDDYILNKE
GIKEDINNKLNYPVIVKPSVLGSSIGINVAYNVSQIEKYIEEAFEYDLTVVVEKFIKAREIECAVIGNDQIKIFTPGEIV
VQDFIFYDYDAKYSTVPGDSIVFNIPAHLDMKHLLDIKEYAFLTYKYLELRGMARIDFLISKDTNLLYVNEVNTIPGFTD
ISMFAKMCEHDGLSYESLVDKLITLAFESYKKRKDKIDFTRLES
>Mature_364_residues
MKKNLMLIFGGVSFEHEISLRSAYGIYSSLLKLDKYNVFSVFVDKVTGIWYLLDSVPSSAELIKRHTTSIVNFIPGCGIF
VNNKSLEIDVIFPIIHGRTGEDGAIQGFVKMMDIPCVGAGILGSAISINKYFCKVLLKSFNIPVVSFIGFKKDDYILNKE
GIKEDINNKLNYPVIVKPSVLGSSIGINVAYNVSQIEKYIEEAFEYDLTVVVEKFIKAREIECAVIGNDQIKIFTPGEIV
VQDFIFYDYDAKYSTVPGDSIVFNIPAHLDMKHLLDIKEYAFLTYKYLELRGMARIDFLISKDTNLLYVNEVNTIPGFTD
ISMFAKMCEHDGLSYESLVDKLITLAFESYKKRKDKIDFTRLES

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=362, Percent_Identity=35.9116022099448, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1786280, Length=353, Percent_Identity=27.7620396600567, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_BORDL (B5RL25)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002221860.1
- EnsemblBacteria:   EBBORT00000001243
- GeneID:   6918282
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_198
- GeneTree:   EBGT00050000007083
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK14570
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 41205; Mature: 41205

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNLMLIFGGVSFEHEISLRSAYGIYSSLLKLDKYNVFSVFVDKVTGIWYLLDSVPSSA
CCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHH
ELIKRHTTSIVNFIPGCGIFVNNKSLEIDVIFPIIHGRTGEDGAIQGFVKMMDIPCVGAG
HHHHHHHHHHHHHCCCCCEEECCCEEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCC
ILGSAISINKYFCKVLLKSFNIPVVSFIGFKKDDYILNKEGIKEDINNKLNYPVIVKPSV
HHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCHHHHHCCCCCCEEECCHH
LGSSIGINVAYNVSQIEKYIEEAFEYDLTVVVEKFIKAREIECAVIGNDQIKIFTPGEIV
CCCCCCEEEEECHHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEEEECCCEEEEEECCHHE
VQDFIFYDYDAKYSTVPGDSIVFNIPAHLDMKHLLDIKEYAFLTYKYLELRGMARIDFLI
EEEHEEEECCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
SKDTNLLYVNEVNTIPGFTDISMFAKMCEHDGLSYESLVDKLITLAFESYKKRKDKIDFT
ECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
RLES
ECCC
>Mature Secondary Structure
MKKNLMLIFGGVSFEHEISLRSAYGIYSSLLKLDKYNVFSVFVDKVTGIWYLLDSVPSSA
CCCCEEEEECCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHH
ELIKRHTTSIVNFIPGCGIFVNNKSLEIDVIFPIIHGRTGEDGAIQGFVKMMDIPCVGAG
HHHHHHHHHHHHHCCCCCEEECCCEEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCC
ILGSAISINKYFCKVLLKSFNIPVVSFIGFKKDDYILNKEGIKEDINNKLNYPVIVKPSV
HHCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCCCCHHHHHCCCCCCEEECCHH
LGSSIGINVAYNVSQIEKYIEEAFEYDLTVVVEKFIKAREIECAVIGNDQIKIFTPGEIV
CCCCCCEEEEECHHHHHHHHHHHHHCHHHHHHHHHHHHCCEEEEEECCCEEEEEECCHHE
VQDFIFYDYDAKYSTVPGDSIVFNIPAHLDMKHLLDIKEYAFLTYKYLELRGMARIDFLI
EEEHEEEECCCCEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
SKDTNLLYVNEVNTIPGFTDISMFAKMCEHDGLSYESLVDKLITLAFESYKKRKDKIDFT
ECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
RLES
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA