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The map label for this gene is tsf

Identifier: 203284047

GI number: 203284047

Start: 130605

End: 131441

Strand: Reverse

Name: tsf

Synonym: BDU_125

Alternate gene names: 203284047

Gene position: 131441-130605 (Counterclockwise)

Preceding gene: 203284048

Following gene: 203284046

Centisome position: 14.11

GC content: 30.35

Gene sequence:

>837_bases
ATGAGTATTAGTCCTCAAGAAGTAAAAAAACTCAGAGATGCAACTGGTGCTGGATTTGGTGATTGTAAAAAGGCATTAAG
TGTTGCTGGTGGTGATTTTGAATTAGCTAAGAAAAAGCTTAAGGAGATGGGTATTGTATCTGCTGATAAGAGAAGTGGTA
GAGATGCTAAGGAAGGGAGAGTTTTTTCTTATGTTAATACAGAAAGAGTGGGTCTTTTACTTATTGCATGCGAGACAGAT
TTTGTTGCTATGAATAGTGATTTTGTGGCTTTTGGAAATTCTTTGATAAAACAATTAGTCGAAAGTGGTAGAGATATTTT
AGATGAACATCAAGAACTTGAAATTAAAAATTTGGCAGCCACAATTAAAGAAAATATTTATGTAAGTAAAGTTTATATTT
CTAACATTGCATCTAATGAGCTTGTGAAAAATTATCTTCATGGGGATCATTCTAAGATAGGTGTGTTTGTTAAATTTAAA
ATAGATGATGTTTTAAAAATGCAAGATGAAAAATTAAATAATTTTGCAATGGATTTAGCTTTGCATGTGGCAGCTTTTTC
TCCTCTTTATTTAAGTGTTAATGATATTTGTCTTAATTATATTAAAGAACAGGAAGAAATTTTTATGAGACAAATGGAAT
CTAGTGGAAAACCTGAAAATGTAGTTAAAGGGATAATATCGGGAAAACTTAAAAAACATTTAGGAGAAATTGCCCTCTTG
GAACAAGGGTTTGTAAAGGATGATAAACTTACTGTTAAAGAAAAAATAGAAGAGGTTTCTAAGTTGATTTTAAGTAAAAT
AGAGGTGGTGGAATTTAAGTATTTAAGTGTTGGATAA

Upstream 100 bases:

>100_bases
CATTCAAATAGTTGAAAATTTGAATGAAGAAGATTTAATGAGCGAAATTGAAGTAAAGAATGAAAGGAAAGAATCATAAA
ATGTATATAAGGAGAGTAAT

Downstream 100 bases:

>100_bases
TAATTTGTGATTCTTTTGATTTTTTAAGGAGGTTTGAATGGAGGAATATAAAGCTTTATTAGATGAAAAGATGGGAAGGG
TTGTTTTATCTCTTGAGAGT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSISPQEVKKLRDATGAGFGDCKKALSVAGGDFELAKKKLKEMGIVSADKRSGRDAKEGRVFSYVNTERVGLLLIACETD
FVAMNSDFVAFGNSLIKQLVESGRDILDEHQELEIKNLAATIKENIYVSKVYISNIASNELVKNYLHGDHSKIGVFVKFK
IDDVLKMQDEKLNNFAMDLALHVAAFSPLYLSVNDICLNYIKEQEEIFMRQMESSGKPENVVKGIISGKLKKHLGEIALL
EQGFVKDDKLTVKEKIEEVSKLILSKIEVVEFKYLSVG

Sequences:

>Translated_278_residues
MSISPQEVKKLRDATGAGFGDCKKALSVAGGDFELAKKKLKEMGIVSADKRSGRDAKEGRVFSYVNTERVGLLLIACETD
FVAMNSDFVAFGNSLIKQLVESGRDILDEHQELEIKNLAATIKENIYVSKVYISNIASNELVKNYLHGDHSKIGVFVKFK
IDDVLKMQDEKLNNFAMDLALHVAAFSPLYLSVNDICLNYIKEQEEIFMRQMESSGKPENVVKGIISGKLKKHLGEIALL
EQGFVKDDKLTVKEKIEEVSKLILSKIEVVEFKYLSVG
>Mature_277_residues
SISPQEVKKLRDATGAGFGDCKKALSVAGGDFELAKKKLKEMGIVSADKRSGRDAKEGRVFSYVNTERVGLLLIACETDF
VAMNSDFVAFGNSLIKQLVESGRDILDEHQELEIKNLAATIKENIYVSKVYISNIASNELVKNYLHGDHSKIGVFVKFKI
DDVLKMQDEKLNNFAMDLALHVAAFSPLYLSVNDICLNYIKEQEEIFMRQMESSGKPENVVKGIISGKLKKHLGEIALLE
QGFVKDDKLTVKEKIEEVSKLILSKIEVVEFKYLSVG

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Escherichia coli, GI1786366, Length=260, Percent_Identity=31.9230769230769, Blast_Score=121, Evalue=5e-29,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_BORDL (B5RLJ6)

Other databases:

- EMBL:   CP000976
- RefSeq:   YP_002221787.1
- EnsemblBacteria:   EBBORT00000001319
- GeneID:   6918209
- GenomeReviews:   CP000976_GR
- KEGG:   bdu:BDU_125
- GeneTree:   EBGT00050000007228
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 31000; Mature: 30869

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISPQEVKKLRDATGAGFGDCKKALSVAGGDFELAKKKLKEMGIVSADKRSGRDAKEGR
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
VFSYVNTERVGLLLIACETDFVAMNSDFVAFGNSLIKQLVESGRDILDEHQELEIKNLAA
EEEEECCCCEEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
TIKENIYVSKVYISNIASNELVKNYLHGDHSKIGVFVKFKIDDVLKMQDEKLNNFAMDLA
HHHHCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHH
LHVAAFSPLYLSVNDICLNYIKEQEEIFMRQMESSGKPENVVKGIISGKLKKHLGEIALL
HHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
EQGFVKDDKLTVKEKIEEVSKLILSKIEVVEFKYLSVG
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECC
>Mature Secondary Structure 
SISPQEVKKLRDATGAGFGDCKKALSVAGGDFELAKKKLKEMGIVSADKRSGRDAKEGR
CCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
VFSYVNTERVGLLLIACETDFVAMNSDFVAFGNSLIKQLVESGRDILDEHQELEIKNLAA
EEEEECCCCEEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
TIKENIYVSKVYISNIASNELVKNYLHGDHSKIGVFVKFKIDDVLKMQDEKLNNFAMDLA
HHHHCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHH
LHVAAFSPLYLSVNDICLNYIKEQEEIFMRQMESSGKPENVVKGIISGKLKKHLGEIALL
HHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
EQGFVKDDKLTVKEKIEEVSKLILSKIEVVEFKYLSVG
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA