Click here to switch to the map view.

The map label for this gene is dnaB [H]

Identifier: 203284036

GI number: 203284036

Start: 120767

End: 122161

Strand: Reverse

Name: dnaB [H]

Synonym: BDU_114

Alternate gene names: 203284036

Gene position: 122161-120767 (Counterclockwise)

Preceding gene: 203284037

Following gene: 203284034

Centisome position: 13.11

GC content: 30.9

Gene sequence:

>1395_bases
GTGGCTTTTACTTCGATAAGCACAACCTCTACACTTCTTTTTAATGAAGGTGCAGAAAAGGCAGTTATTTCAAGTATATT
TTATAATCCGGGAAAAGTAGAAGAGGCTTTATTATATTTAAAGCCGGATGACTTTTATAATCAAGATCATGAAATGATTT
TTAAAGCTATGATTTCTCTTTATGAGAAAAGAGAAAATATTGATCCGATAACTGTATTTGAGGAGGTTTCTACTATAACT
CCTAAGTCACAACTTGTAAATAATTTGAAAGCTTTAACGGGTTTACAAGATTATTTAAGTTTTCTTTCAGGTTATCTTCC
AACTGATAAGACTATAAATATTTATGCAAAAATTGTTAAGGAACATCGTATTAGGAGAGATATTGCAAAAATTTCTAGAG
AACTAAATGATTTGGCTAATGATTCTACAAAAAAAGTTGAACAATTTGTGGAAGAAGCACAAAGACAAGTTCTTTCAATT
GAATTAGATTGTTCTAATAAAAATCTTAATCATGCAAAGGTAATTGCTGAAAGAGTTCATGCTGAAATATATGAGAGGAG
TGTTCAAAAACGAGAAGTTGATTTTGGCATTCCAAGTGGTTTTAAAAAAGTAGATAGTCTTATTGGTGGATTTAGGGAGA
GTGATTTTATCATTATTGGTGCTCGTCCTAGTGTTGGAAAGACAGCATTTGCGTTAAATATTGCATCCAGTATAGCCTTA
AAGGGTGATAAAAAACGAAGAGTAGGATTTTTTTCACTTGAAATGACCTCTGATGCTTTAATTAAAAGAATAATAGCATC
TCAGGCTAATATTGATGGTTTTAAGATTCAAAATAGTATTTTGTCAGGACATGAAATTAAGGCAATTAATGATGTTGTAA
ATGATATTAGTAATTCTGAATTTTATATTGAGGATACTGCAAATATTAGTTTGTTAACGCTTGCAACGCAGGCTAGAAAA
CTTAAAAGATTTTCAGGTATAGATATACTTTTTGTTGATTATATTAGTCTTATTTCTCTTGAAAGTAAAAATGTTCCAAG
GCATGAGCAGGTAGCTTCAATTAGTAAAGCTTTAAAAGAGCTTGCTAGAGAACTTAAAATACCAATTGTTGCTCTTTCAC
AGCTTACACGAGATACTGAGGGACGTGAGCCTAGTCTTGCTAGTTTAAGAGAATCAGGTGCATTAGAACAAGATGCAGAT
ATTGTTATTTTACTTCATAGAGATAAGGATTTAAAGAATAATTCTGATGATGATGATGGAATTGTGAATGCTATTGATAC
TAAAGTTATTGTTGCTAAACATAGAAATGGTCCTACGGGTAGGGTTGATATATTATTTTTGCCGCATGTTGTTAAATTCG
TTAATAAAGAACATGAAATGGACTATTATTTATAG

Upstream 100 bases:

>100_bases
TTCTGTAATTACGGTTGAGATCAAAAGGGAAGAGAAAAAAAATTCTCTTAAAAAGTCTAAAAGTGTTGAAAAAGAAGTTT
AAGGAAGAGGGGCATAGCTT

Downstream 100 bases:

>100_bases
ATAGATTATATTTTTTTGATGTTTAAGGTGTTTGAGGCATTAAATATTAATTTTTATGCCGATTCCGAGATAGTTGCAAA
TGAATTTATGTTTTTCTAAC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MAFTSISTTSTLLFNEGAEKAVISSIFYNPGKVEEALLYLKPDDFYNQDHEMIFKAMISLYEKRENIDPITVFEEVSTIT
PKSQLVNNLKALTGLQDYLSFLSGYLPTDKTINIYAKIVKEHRIRRDIAKISRELNDLANDSTKKVEQFVEEAQRQVLSI
ELDCSNKNLNHAKVIAERVHAEIYERSVQKREVDFGIPSGFKKVDSLIGGFRESDFIIIGARPSVGKTAFALNIASSIAL
KGDKKRRVGFFSLEMTSDALIKRIIASQANIDGFKIQNSILSGHEIKAINDVVNDISNSEFYIEDTANISLLTLATQARK
LKRFSGIDILFVDYISLISLESKNVPRHEQVASISKALKELARELKIPIVALSQLTRDTEGREPSLASLRESGALEQDAD
IVILLHRDKDLKNNSDDDDGIVNAIDTKVIVAKHRNGPTGRVDILFLPHVVKFVNKEHEMDYYL

Sequences:

>Translated_464_residues
MAFTSISTTSTLLFNEGAEKAVISSIFYNPGKVEEALLYLKPDDFYNQDHEMIFKAMISLYEKRENIDPITVFEEVSTIT
PKSQLVNNLKALTGLQDYLSFLSGYLPTDKTINIYAKIVKEHRIRRDIAKISRELNDLANDSTKKVEQFVEEAQRQVLSI
ELDCSNKNLNHAKVIAERVHAEIYERSVQKREVDFGIPSGFKKVDSLIGGFRESDFIIIGARPSVGKTAFALNIASSIAL
KGDKKRRVGFFSLEMTSDALIKRIIASQANIDGFKIQNSILSGHEIKAINDVVNDISNSEFYIEDTANISLLTLATQARK
LKRFSGIDILFVDYISLISLESKNVPRHEQVASISKALKELARELKIPIVALSQLTRDTEGREPSLASLRESGALEQDAD
IVILLHRDKDLKNNSDDDDGIVNAIDTKVIVAKHRNGPTGRVDILFLPHVVKFVNKEHEMDYYL
>Mature_463_residues
AFTSISTTSTLLFNEGAEKAVISSIFYNPGKVEEALLYLKPDDFYNQDHEMIFKAMISLYEKRENIDPITVFEEVSTITP
KSQLVNNLKALTGLQDYLSFLSGYLPTDKTINIYAKIVKEHRIRRDIAKISRELNDLANDSTKKVEQFVEEAQRQVLSIE
LDCSNKNLNHAKVIAERVHAEIYERSVQKREVDFGIPSGFKKVDSLIGGFRESDFIIIGARPSVGKTAFALNIASSIALK
GDKKRRVGFFSLEMTSDALIKRIIASQANIDGFKIQNSILSGHEIKAINDVVNDISNSEFYIEDTANISLLTLATQARKL
KRFSGIDILFVDYISLISLESKNVPRHEQVASISKALKELARELKIPIVALSQLTRDTEGREPSLASLRESGALEQDADI
VILLHRDKDLKNNSDDDDGIVNAIDTKVIVAKHRNGPTGRVDILFLPHVVKFVNKEHEMDYYL

Specific function: Participates In Initiation And Elongation During Chromosome Replication; It Exhibits DNA-Dependent ATPase Activity And Contains Distinct Active Sites For ATP Binding, DNA Binding, And Interaction With DNAc Protein, Primase, And Other Prepriming Proteins.

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=447, Percent_Identity=31.5436241610738, Blast_Score=202, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52121; Mature: 51990

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFTSISTTSTLLFNEGAEKAVISSIFYNPGKVEEALLYLKPDDFYNQDHEMIFKAMISL
CCCCCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHEEEEECCCHHCCCHHHHHHHHHHHH
YEKRENIDPITVFEEVSTITPKSQLVNNLKALTGLQDYLSFLSGYLPTDKTINIYAKIVK
HHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
EHRIRRDIAKISRELNDLANDSTKKVEQFVEEAQRQVLSIELDCSNKNLNHAKVIAERVH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHH
AEIYERSVQKREVDFGIPSGFKKVDSLIGGFRESDFIIIGARPSVGKTAFALNIASSIAL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHC
KGDKKRRVGFFSLEMTSDALIKRIIASQANIDGFKIQNSILSGHEIKAINDVVNDISNSE
CCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCEEEHHHHCCCCHHHHHHHHHHHCCCCE
FYIEDTANISLLTLATQARKLKRFSGIDILFVDYISLISLESKNVPRHEQVASISKALKE
EEEECCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LARELKIPIVALSQLTRDTEGREPSLASLRESGALEQDADIVILLHRDKDLKNNSDDDDG
HHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCC
IVNAIDTKVIVAKHRNGPTGRVDILFLPHVVKFVNKEHEMDYYL
HHHHCCCEEEEEECCCCCCCEEEEEEHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AFTSISTTSTLLFNEGAEKAVISSIFYNPGKVEEALLYLKPDDFYNQDHEMIFKAMISL
CCCCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHEEEEECCCHHCCCHHHHHHHHHHHH
YEKRENIDPITVFEEVSTITPKSQLVNNLKALTGLQDYLSFLSGYLPTDKTINIYAKIVK
HHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
EHRIRRDIAKISRELNDLANDSTKKVEQFVEEAQRQVLSIELDCSNKNLNHAKVIAERVH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHH
AEIYERSVQKREVDFGIPSGFKKVDSLIGGFRESDFIIIGARPSVGKTAFALNIASSIAL
HHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEHHHHHHC
KGDKKRRVGFFSLEMTSDALIKRIIASQANIDGFKIQNSILSGHEIKAINDVVNDISNSE
CCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCEEEHHHHCCCCHHHHHHHHHHHCCCCE
FYIEDTANISLLTLATQARKLKRFSGIDILFVDYISLISLESKNVPRHEQVASISKALKE
EEEECCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LARELKIPIVALSQLTRDTEGREPSLASLRESGALEQDADIVILLHRDKDLKNNSDDDDG
HHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCC
IVNAIDTKVIVAKHRNGPTGRVDILFLPHVVKFVNKEHEMDYYL
HHHHCCCEEEEEECCCCCCCEEEEEEHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 1312531 [H]