Click here to switch to the map view.

The map label for this gene is folD [H]

Identifier: 203283955

GI number: 203283955

Start: 35563

End: 36504

Strand: Reverse

Name: folD [H]

Synonym: BDU_31

Alternate gene names: 203283955

Gene position: 36504-35563 (Counterclockwise)

Preceding gene: 203283958

Following gene: 203283954

Centisome position: 3.92

GC content: 31.0

Gene sequence:

>942_bases
ATGCTTCAAACTAGATTGGTGGAGGGGAGGGCTTATGTGAGCAGTGTTTTTGATGGTAAAATTTTTGCAAATCGATATTA
TTTGTTGTTAAAAGAGTTCTTGATCTATCATAATTTAGTAAATAAAATTTCTCTTAAAGTAATTTTGGCAAATAATAGTC
CTGCAAGTGAACTTTATGTTTCTGTTAAGGAGAGAGTATCTAAAGAAATTGGTATTAACTTTAATGTTATTAGGTTGAGT
GAGAATTCGCATCAAGATGACATTTTGCGTTTAATTGAGATTGAAAATACAAATGATTATACTGATGGGATTATTGTTCA
GTTGCCACTTGCTAATAAAATAGATGTGAATATGATTTTAAATAGCATATTAAGTATAAAGGATGTAGATGGACTTTCTT
TTGTGAATTTGGGTAAATTAGTTTCGGGAGATAAGAGAGGGTTTATTCCTTGTACGGCTCTTGCTGTATTGAAAATTTTA
TTTGATTATAAAATAGAAATTTCTGGCAAAACTGTTGTTGTTATTGGTAGAAGCTTGCTTGTTGGGAGAGCCATTTCTAT
TTTGCTCTCTTGTAAGCCTTATAATGCAACTGTTATTGTTTGTCATAGTAAAACTGTCAATTTAGATTTTTATGTAAAAC
AAGCAGATGTTATTATTTCTGCTGTTGGAAAACCTAAATTAATAGATGCTAGTATGATAGTTGGACATCCTTATGTTATT
GATATTGGTATTTCTAGAGTAGATGTTAATAATAGTAGTGTTCTTGTAGGTGATGTTGATTTTGAGGCAGTAAAGGATCG
TGTTAGGTTCATTACTCCTGTAATAGGGGGAGTTGGGCCTGTTACAGTTCTTATGTTGATGTTTAATACAATTAAAGCCT
ATTTAATTAGGCGTAAGAAGTTTGATCTTTTAGAAAAATTGATGAAATTGGTGGAGGTTTGA

Upstream 100 bases:

>100_bases
TTTTAAAAGTTATTATATTGAAAAATAGTTTTTATTACAATTAATTTTTATATGCATTTATTCATGTTTATACTTAAAGT
ACAATATAGTATAATGGATA

Downstream 100 bases:

>100_bases
ATGTCTGGTCACAGTAAATGGTCAACTATAAAGAGAAAGAAAGGTACTCTTGATGCTAAGAGAAATAAGATTTTTACTAA
GTTAATTCGTGAAATAAGTA

Product: methylenetetrahydrofolate dehydrogenase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLQTRLVEGRAYVSSVFDGKIFANRYYLLLKEFLIYHNLVNKISLKVILANNSPASELYVSVKERVSKEIGINFNVIRLS
ENSHQDDILRLIEIENTNDYTDGIIVQLPLANKIDVNMILNSILSIKDVDGLSFVNLGKLVSGDKRGFIPCTALAVLKIL
FDYKIEISGKTVVVIGRSLLVGRAISILLSCKPYNATVIVCHSKTVNLDFYVKQADVIISAVGKPKLIDASMIVGHPYVI
DIGISRVDVNNSSVLVGDVDFEAVKDRVRFITPVIGGVGPVTVLMLMFNTIKAYLIRRKKFDLLEKLMKLVEV

Sequences:

>Translated_313_residues
MLQTRLVEGRAYVSSVFDGKIFANRYYLLLKEFLIYHNLVNKISLKVILANNSPASELYVSVKERVSKEIGINFNVIRLS
ENSHQDDILRLIEIENTNDYTDGIIVQLPLANKIDVNMILNSILSIKDVDGLSFVNLGKLVSGDKRGFIPCTALAVLKIL
FDYKIEISGKTVVVIGRSLLVGRAISILLSCKPYNATVIVCHSKTVNLDFYVKQADVIISAVGKPKLIDASMIVGHPYVI
DIGISRVDVNNSSVLVGDVDFEAVKDRVRFITPVIGGVGPVTVLMLMFNTIKAYLIRRKKFDLLEKLMKLVEV
>Mature_313_residues
MLQTRLVEGRAYVSSVFDGKIFANRYYLLLKEFLIYHNLVNKISLKVILANNSPASELYVSVKERVSKEIGINFNVIRLS
ENSHQDDILRLIEIENTNDYTDGIIVQLPLANKIDVNMILNSILSIKDVDGLSFVNLGKLVSGDKRGFIPCTALAVLKIL
FDYKIEISGKTVVVIGRSLLVGRAISILLSCKPYNATVIVCHSKTVNLDFYVKQADVIISAVGKPKLIDASMIVGHPYVI
DIGISRVDVNNSSVLVGDVDFEAVKDRVRFITPVIGGVGPVTVLMLMFNTIKAYLIRRKKFDLLEKLMKLVEV

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=263, Percent_Identity=38.7832699619772, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI222418558, Length=259, Percent_Identity=42.4710424710425, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI94721354, Length=274, Percent_Identity=41.2408759124088, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI36796743, Length=177, Percent_Identity=27.683615819209, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1786741, Length=253, Percent_Identity=39.9209486166008, Blast_Score=163, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17568735, Length=260, Percent_Identity=39.2307692307692, Blast_Score=170, Evalue=9e-43,
Organism=Saccharomyces cerevisiae, GI6319558, Length=270, Percent_Identity=40.3703703703704, Blast_Score=177, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321643, Length=264, Percent_Identity=38.6363636363636, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322933, Length=195, Percent_Identity=29.2307692307692, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17136818, Length=302, Percent_Identity=39.4039735099338, Blast_Score=186, Evalue=3e-47,
Organism=Drosophila melanogaster, GI17136816, Length=302, Percent_Identity=39.4039735099338, Blast_Score=185, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24645718, Length=271, Percent_Identity=38.0073800738007, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17137370, Length=271, Percent_Identity=38.0073800738007, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI62472483, Length=270, Percent_Identity=38.1481481481481, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI45551871, Length=270, Percent_Identity=38.1481481481481, Blast_Score=158, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 34830; Mature: 34830

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00589 PTS_HPR_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQTRLVEGRAYVSSVFDGKIFANRYYLLLKEFLIYHNLVNKISLKVILANNSPASELYV
CCCCEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHH
SVKERVSKEIGINFNVIRLSENSHQDDILRLIEIENTNDYTDGIIVQLPLANKIDVNMIL
HHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCHHHHHH
NSILSIKDVDGLSFVNLGKLVSGDKRGFIPCTALAVLKILFDYKIEISGKTVVVIGRSLL
HHHHHHCCCCCCCCEEHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEECCCEEEEECCHHH
VGRAISILLSCKPYNATVIVCHSKTVNLDFYVKQADVIISAVGKPKLIDASMIVGHPYVI
HHHHHHHEEECCCCCEEEEEEECEEEEEEEEEEEHEEEEEECCCCCEEECEEEECCCEEE
DIGISRVDVNNSSVLVGDVDFEAVKDRVRFITPVIGGVGPVTVLMLMFNTIKAYLIRRKK
EEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FDLLEKLMKLVEV
HHHHHHHHHHHCC
>Mature Secondary Structure
MLQTRLVEGRAYVSSVFDGKIFANRYYLLLKEFLIYHNLVNKISLKVILANNSPASELYV
CCCCEEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHHH
SVKERVSKEIGINFNVIRLSENSHQDDILRLIEIENTNDYTDGIIVQLPLANKIDVNMIL
HHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEEEECCCCCCCCEEEEECCCCCCHHHHHH
NSILSIKDVDGLSFVNLGKLVSGDKRGFIPCTALAVLKILFDYKIEISGKTVVVIGRSLL
HHHHHHCCCCCCCCEEHHHHHCCCCCCCCHHHHHHHHHHHHHEEEEECCCEEEEECCHHH
VGRAISILLSCKPYNATVIVCHSKTVNLDFYVKQADVIISAVGKPKLIDASMIVGHPYVI
HHHHHHHEEECCCCCEEEEEEECEEEEEEEEEEEHEEEEEECCCCCEEECEEEECCCEEE
DIGISRVDVNNSSVLVGDVDFEAVKDRVRFITPVIGGVGPVTVLMLMFNTIKAYLIRRKK
EEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
FDLLEKLMKLVEV
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]