Click here to switch to the map view.

The map label for this gene is MPH1/MUS81 [C]

Identifier: 20095114

GI number: 20095114

Start: 1678882

End: 1681107

Strand: Reverse

Name: MPH1/MUS81 [C]

Synonym: MK1678

Alternate gene names: 20095114

Gene position: 1681107-1678882 (Counterclockwise)

Preceding gene: 20095115

Following gene: 20095108

Centisome position: 99.18

GC content: 56.33

Gene sequence:

>2226_bases
ATGCCTGCATGGAGACGGGTGAATTGGAACTTCCTGGAGCGTCGCGAGTACCAAGTGTCCGTAGCAGCCGAGATTCTCGA
TTCAATGGACAACACTCTGGTGGTGATACCCACCGGGCTAGGTAAGACCGCGATCGGTGTGATGGTACTCTCGGAACTCG
TGGACGAAGGCCGGGCCGTGTTCTTAGCTCCCACCGTACCGCTAGTGAATCAACACGCCCGATTCATAGAGCGAGCTACA
CGAGGATTGGACGTGAAAGCACTCACGGGTAGGGTGCGACCGGAACGAAGGAAAGTCGAGTGGAAAAAATCCGACGTAAT
TGTTGCAACACCGCATGTAATCCGCAATGACATTATAGAGGGTAGAATTGACCCTGATGAGGCATCCGTTGTGATATTTG
ATGAGGCGCATAGAGCGGTTGGAGGTTACCCATATGTGTATGTATCAAAGGAGTTTAATTGCCTGAAAGTTGGCCTTACA
GCGTCGCCTGGATCGGATGTGAAACGCATAAAAGAGGTAGTGCAAAATCTTGGGATAGAACGCATTATAGTTAAAACAGA
GGAGGATCCAGACGTTAAAAAGTACTTAGGAAGAGTGAAAGTTGAGTGGGTTGACGTCGAACTGCCGGAATGGTTCGACA
ACGCTAGACGGGAGCTGCAGCGGGCTTTCGAGCGCAGGCTGGAGTTACTGGAGGACATGGGGTTCCTACAATCCTCGCGT
AACGTGTGGGTGGGAAAGCTGCTGTCGCTCAGGGAGGAGATCAGGGAACAGATGGCGAAGCGCCGAGAACGCGCCAGTTG
GTGCTCACGAGCTCTCGGAGTCGTAGCGGAAGCCCTCCGGATAGCTAGGGCGAGGGAGATCCTGGAAACCCAAGGAATCG
AACCTTTCCTACGGTACGTCGAGCGCCTTACGGAACGCAAACGGTCGAGCGGCGGTTCCTCGCTACGGAGGATACTCGGA
GATCCGAACTTCCAGCGCGCCGTACGAGAGTGCAAATCGGCGTCACTCCGGGACGAACCGGATCACCCGAAACTACCCGA
GGTTGAGGAGTTAGTGAAAGACGTCGAGTCCGCGCTGGTGTTCACGCAATACGTGGACACCGCCAAGCTGATCGCCGATT
ACCTCAAGGAGATTGGGATATCCGTCGGGGTGTTGCTCGGCAAGGAACACATGAAGGAGCACGAGCAGCTGGACGTCATA
AAATCGATCAAACGGGGAGAGTGTCGTGTTCTCGTGTCGACGAGCGTAGGGGAGGAGGGACTGGACCTTCCGACCTGCGA
GGAGGTGGTGCTGTACGAGCCGGTTCCCTCGGAAATAAGGACGATCCAGCGGATCGGACGTACCGCGAGGGATGGTGCCG
TGGGTAACGCTCACGTTCTCGTCGCTAGAGGTTCCTTTCCCACCTTGGACGAGATCTACTTTCACGTCGCGAGGCGTCGT
GAGAAGAAGATGTTGGAGGCAGTGATGCGCGTGCAGGAGTGGTTGCGGAAGAGGAAAGGGAAGACGACCGCTACGAGCAA
AAACCTCAGAAAGTTGCGTTCGAGGGCGAAGACGCTAGATCAGTTCGTAGGTGGGGGTCGTAGCAAACGCGAGCGCGATG
AAGTGCGTCCACCGAGCAGGGCTCCCGTCATCGTAGTGGACTCGCGTGAACTCAACACCAAGGTTGTGGAGCACCTCAGG
AGAAAGCCCGTGGTCCTAGAGAGAGACACGTTAGAACTGGCGGACTACGTCGTAGGTGAGGGCGTCGGTGTGGAGCGCAA
GTCGGAATCCGATTTCGCCAGGTCTCTCCTGGACGGTAGGTTAATGGATCAAGCTCGAGAGATGACCCGAGAGTTCGACC
GTGCCGTAATTATCGTGGAGGGGAACCCGAGGAGGGAGATCGAGCCGGAAGCCGTTGATGGAGCACTGGCGACGCTCGCC
GTCGACTTCGGAATCTCTGTGCTTCAGTCGGCGGGACCGGAGGAAACTGCGGAGCTACTCTACCGGATGGCCAAAAGGTT
CGAGGAGCGACAGAGGCCGAGGCCACGGAAGCGTAGGTCAACCGAGGATCTTCGGGTGGAGATGTTGTCATGTATCCCGG
GAGTTGGACCGGAGCTGGCTCGGAGGCTGTTGGACGAGTTCGGATCCATAGGCGACGTCGTGAACGCTAGTCCATCTGAG
TTGAAACGCGTTAAGGGGATAGGGGAACGGAAGGCCAGGGAGATACGGCGTTTCCTCTGGAGCTAA

Upstream 100 bases:

>100_bases
GCAAGCTCAGTGAACTGCGCCGTGAGATAGTGTCCTCCGTGGAGGATCCGGAAAAGGCCGTCCGGGAGTTCCGGCGTCGG
GCCGAGGAGGTGATCCGGGA

Downstream 100 bases:

>100_bases
GCCGTCGCGCGAATCGTTCAAGTTCTCATAACAATTCGGCCGGGGGGAAGAAAGGCTTAGAGCTCTCCACCCTTGGGCAT
GGAGTACGTGATGGCCTTTT

Product: Hef nuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 741; Mature: 740

Protein sequence:

>741_residues
MPAWRRVNWNFLERREYQVSVAAEILDSMDNTLVVIPTGLGKTAIGVMVLSELVDEGRAVFLAPTVPLVNQHARFIERAT
RGLDVKALTGRVRPERRKVEWKKSDVIVATPHVIRNDIIEGRIDPDEASVVIFDEAHRAVGGYPYVYVSKEFNCLKVGLT
ASPGSDVKRIKEVVQNLGIERIIVKTEEDPDVKKYLGRVKVEWVDVELPEWFDNARRELQRAFERRLELLEDMGFLQSSR
NVWVGKLLSLREEIREQMAKRRERASWCSRALGVVAEALRIARAREILETQGIEPFLRYVERLTERKRSSGGSSLRRILG
DPNFQRAVRECKSASLRDEPDHPKLPEVEELVKDVESALVFTQYVDTAKLIADYLKEIGISVGVLLGKEHMKEHEQLDVI
KSIKRGECRVLVSTSVGEEGLDLPTCEEVVLYEPVPSEIRTIQRIGRTARDGAVGNAHVLVARGSFPTLDEIYFHVARRR
EKKMLEAVMRVQEWLRKRKGKTTATSKNLRKLRSRAKTLDQFVGGGRSKRERDEVRPPSRAPVIVVDSRELNTKVVEHLR
RKPVVLERDTLELADYVVGEGVGVERKSESDFARSLLDGRLMDQAREMTREFDRAVIIVEGNPRREIEPEAVDGALATLA
VDFGISVLQSAGPEETAELLYRMAKRFEERQRPRPRKRRSTEDLRVEMLSCIPGVGPELARRLLDEFGSIGDVVNASPSE
LKRVKGIGERKAREIRRFLWS

Sequences:

>Translated_741_residues
MPAWRRVNWNFLERREYQVSVAAEILDSMDNTLVVIPTGLGKTAIGVMVLSELVDEGRAVFLAPTVPLVNQHARFIERAT
RGLDVKALTGRVRPERRKVEWKKSDVIVATPHVIRNDIIEGRIDPDEASVVIFDEAHRAVGGYPYVYVSKEFNCLKVGLT
ASPGSDVKRIKEVVQNLGIERIIVKTEEDPDVKKYLGRVKVEWVDVELPEWFDNARRELQRAFERRLELLEDMGFLQSSR
NVWVGKLLSLREEIREQMAKRRERASWCSRALGVVAEALRIARAREILETQGIEPFLRYVERLTERKRSSGGSSLRRILG
DPNFQRAVRECKSASLRDEPDHPKLPEVEELVKDVESALVFTQYVDTAKLIADYLKEIGISVGVLLGKEHMKEHEQLDVI
KSIKRGECRVLVSTSVGEEGLDLPTCEEVVLYEPVPSEIRTIQRIGRTARDGAVGNAHVLVARGSFPTLDEIYFHVARRR
EKKMLEAVMRVQEWLRKRKGKTTATSKNLRKLRSRAKTLDQFVGGGRSKRERDEVRPPSRAPVIVVDSRELNTKVVEHLR
RKPVVLERDTLELADYVVGEGVGVERKSESDFARSLLDGRLMDQAREMTREFDRAVIIVEGNPRREIEPEAVDGALATLA
VDFGISVLQSAGPEETAELLYRMAKRFEERQRPRPRKRRSTEDLRVEMLSCIPGVGPELARRLLDEFGSIGDVVNASPSE
LKRVKGIGERKAREIRRFLWS
>Mature_740_residues
PAWRRVNWNFLERREYQVSVAAEILDSMDNTLVVIPTGLGKTAIGVMVLSELVDEGRAVFLAPTVPLVNQHARFIERATR
GLDVKALTGRVRPERRKVEWKKSDVIVATPHVIRNDIIEGRIDPDEASVVIFDEAHRAVGGYPYVYVSKEFNCLKVGLTA
SPGSDVKRIKEVVQNLGIERIIVKTEEDPDVKKYLGRVKVEWVDVELPEWFDNARRELQRAFERRLELLEDMGFLQSSRN
VWVGKLLSLREEIREQMAKRRERASWCSRALGVVAEALRIARAREILETQGIEPFLRYVERLTERKRSSGGSSLRRILGD
PNFQRAVRECKSASLRDEPDHPKLPEVEELVKDVESALVFTQYVDTAKLIADYLKEIGISVGVLLGKEHMKEHEQLDVIK
SIKRGECRVLVSTSVGEEGLDLPTCEEVVLYEPVPSEIRTIQRIGRTARDGAVGNAHVLVARGSFPTLDEIYFHVARRRE
KKMLEAVMRVQEWLRKRKGKTTATSKNLRKLRSRAKTLDQFVGGGRSKRERDEVRPPSRAPVIVVDSRELNTKVVEHLRR
KPVVLERDTLELADYVVGEGVGVERKSESDFARSLLDGRLMDQAREMTREFDRAVIIVEGNPRREIEPEAVDGALATLAV
DFGISVLQSAGPEETAELLYRMAKRFEERQRPRPRKRRSTEDLRVEMLSCIPGVGPELARRLLDEFGSIGDVVNASPSEL
KRVKGIGERKAREIRRFLWS

Specific function: RNA-Dependent ATPase Activity. Suppressor Of A Mutant Defective In 50s Ribosomal Subunit Assembly. Probably Interacts With 23s Ribosomal RNA. [C]

COG id: COG1111

COG function: function code L; ERCC4-like helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI74959747, Length=512, Percent_Identity=27.734375, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI4885217, Length=350, Percent_Identity=25.1428571428571, Blast_Score=87, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6322192, Length=194, Percent_Identity=36.0824742268041, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI116008407, Length=550, Percent_Identity=27.8181818181818, Blast_Score=144, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR020819
- InterPro:   IPR006166
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR011335
- InterPro:   IPR010994 [H]

Pfam domain/function: PF00270 DEAD; PF02732 ERCC4; PF00271 Helicase_C; PF00633 HHH [H]

EC number: =3.6.4.13 [H]

Molecular weight: Translated: 84426; Mature: 84295

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00690 DEAH_ATP_HELICASE ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAWRRVNWNFLERREYQVSVAAEILDSMDNTLVVIPTGLGKTAIGVMVLSELVDEGRAV
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEE
FLAPTVPLVNQHARFIERATRGLDVKALTGRVRPERRKVEWKKSDVIVATPHVIRNDIIE
EEECCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHCCCCCCCCEEEECCHHHHHHHHC
GRIDPDEASVVIFDEAHRAVGGYPYVYVSKEFNCLKVGLTASPGSDVKRIKEVVQNLGIE
CCCCCCCCCEEEEECHHHHCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCE
RIIVKTEEDPDVKKYLGRVKVEWVDVELPEWFDNARRELQRAFERRLELLEDMGFLQSSR
EEEEECCCCCCHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NVWVGKLLSLREEIREQMAKRRERASWCSRALGVVAEALRIARAREILETQGIEPFLRYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ERLTERKRSSGGSSLRRILGDPNFQRAVRECKSASLRDEPDHPKLPEVEELVKDVESALV
HHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
FTQYVDTAKLIADYLKEIGISVGVLLGKEHMKEHEQLDVIKSIKRGECRVLVSTSVGEEG
HHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
LDLPTCEEVVLYEPVPSEIRTIQRIGRTARDGAVGNAHVLVARGSFPTLDEIYFHVARRR
CCCCCCCCEEEECCCCHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
EKKMLEAVMRVQEWLRKRKGKTTATSKNLRKLRSRAKTLDQFVGGGRSKRERDEVRPPSR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCC
APVIVVDSRELNTKVVEHLRRKPVVLERDTLELADYVVGEGVGVERKSESDFARSLLDGR
CCEEEECCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
LMDQAREMTREFDRAVIIVEGNPRREIEPEAVDGALATLAVDFGISVLQSAGPEETAELL
HHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
YRMAKRFEERQRPRPRKRRSTEDLRVEMLSCIPGVGPELARRLLDEFGSIGDVVNASPSE
HHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHH
LKRVKGIGERKAREIRRFLWS
HHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PAWRRVNWNFLERREYQVSVAAEILDSMDNTLVVIPTGLGKTAIGVMVLSELVDEGRAV
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCEE
FLAPTVPLVNQHARFIERATRGLDVKALTGRVRPERRKVEWKKSDVIVATPHVIRNDIIE
EEECCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHCCCCCCCCEEEECCHHHHHHHHC
GRIDPDEASVVIFDEAHRAVGGYPYVYVSKEFNCLKVGLTASPGSDVKRIKEVVQNLGIE
CCCCCCCCCEEEEECHHHHCCCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHCCCE
RIIVKTEEDPDVKKYLGRVKVEWVDVELPEWFDNARRELQRAFERRLELLEDMGFLQSSR
EEEEECCCCCCHHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NVWVGKLLSLREEIREQMAKRRERASWCSRALGVVAEALRIARAREILETQGIEPFLRYV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
ERLTERKRSSGGSSLRRILGDPNFQRAVRECKSASLRDEPDHPKLPEVEELVKDVESALV
HHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHH
FTQYVDTAKLIADYLKEIGISVGVLLGKEHMKEHEQLDVIKSIKRGECRVLVSTSVGEEG
HHHHHHHHHHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
LDLPTCEEVVLYEPVPSEIRTIQRIGRTARDGAVGNAHVLVARGSFPTLDEIYFHVARRR
CCCCCCCCEEEECCCCHHHHHHHHHCCHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
EKKMLEAVMRVQEWLRKRKGKTTATSKNLRKLRSRAKTLDQFVGGGRSKRERDEVRPPSR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCC
APVIVVDSRELNTKVVEHLRRKPVVLERDTLELADYVVGEGVGVERKSESDFARSLLDGR
CCEEEECCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
LMDQAREMTREFDRAVIIVEGNPRREIEPEAVDGALATLAVDFGISVLQSAGPEETAELL
HHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
YRMAKRFEERQRPRPRKRRSTEDLRVEMLSCIPGVGPELARRLLDEFGSIGDVVNASPSE
HHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHH
LKRVKGIGERKAREIRRFLWS
HHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]