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The map label for this gene is PrsA

Identifier: 20095103

GI number: 20095103

Start: 1669535

End: 1670410

Strand: Direct

Name: PrsA

Synonym: MK1667

Alternate gene names: 20095103

Gene position: 1669535-1670410 (Clockwise)

Preceding gene: 20095102

Following gene: 20095104

Centisome position: 98.5

GC content: 62.33

Gene sequence:

>876_bases
TTGATAGTCCTCGCCACATCGGAGGGTGTCAAGCTGGGTCGGCGTCTCGCAGAGGAGCTCGACGCGGAGCTGGCGCCGGT
CGAGGAGGACAGGTTCCCCGACGGTGAGCAGATTGTAAGGGTTCCTCCGGAGCTCGACGGGACGGTAGTCGTCGTACACT
CGATGAGCCCACCCCAGGACGAGAACCTCGTGAAGGCGATAATCACTCTCGACGCCGCTCGGGAGAACGGGGCCGAGGAA
GTCATCGCCATAGTCCCTTACATGGCCTACTCCAGGCAAGATCGTAGATTCGAACCCGGAGAGCCCGTTTCGTTCCGCGC
CGTCGCTAGGGCAGTGTCCGCGAACGCCGACGCTCTAATAACCGTCGACCTCCACGAGCCGGGCACGCTGAAGTACTTCG
ACGTACCCGCCGAGAACGTCAGCGCGGCTGAGGAGCTGGGCAAGTACCTGGCAGAAAGGTTCGAGGGAGAGGATCTCGTG
GTCATCGGACCGGACGAAGGGGCCCGAGAACTGGCCCGCGAAGTCGCCTCGATATGTGGGGTTGAATACGACCATCTGGA
GAAAAAGCGGCTCAGCGGGGACGAGGTCGAGATTCATCCGAAAGAGCTGGACGTCGAGGGGCGGACGGTGGTACTGGTCG
ACGACATGATCGACACCGGAGGGACCATGGTTGAAGCCGCCCGAGCGCTTCGCGATCAAGGTGCCGGGACGCTCTATGCC
GCGTGCACCCACGCGCTCCTCACCAGGAACGCCGCCACTAGGCTTTTGGCCTCAGGATTCGAAGATATCATAGCCACCGA
CACCGTCCCCAACCCGTTCGAGAAGGTGAGTGTGGCCCCACCGGTAGCCGAGGCTGTTGAGAATCTCAGCGGGTGA

Upstream 100 bases:

>100_bases
CGTCAACTGCCTCCACGACATCCACAAGGGTGATTGGACACCTCGCGAGGTGAAACCCGAAGAGTTGACTCGCTACGGAG
GATGATGGGGGAGAGACGTG

Downstream 100 bases:

>100_bases
GACCCGGGAGAAACACTTAAAATCCCGCCTCATACTTGTGCAAGTGGGCAAGGCGCCGGGGCGGCCGAGCCCGGTCCAAG
GCGCGGGACTGCTAATCCCG

Product: phosphoribosylpyrophosphate synthetase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MIVLATSEGVKLGRRLAEELDAELAPVEEDRFPDGEQIVRVPPELDGTVVVVHSMSPPQDENLVKAIITLDAARENGAEE
VIAIVPYMAYSRQDRRFEPGEPVSFRAVARAVSANADALITVDLHEPGTLKYFDVPAENVSAAEELGKYLAERFEGEDLV
VIGPDEGARELAREVASICGVEYDHLEKKRLSGDEVEIHPKELDVEGRTVVLVDDMIDTGGTMVEAARALRDQGAGTLYA
ACTHALLTRNAATRLLASGFEDIIATDTVPNPFEKVSVAPPVAEAVENLSG

Sequences:

>Translated_291_residues
MIVLATSEGVKLGRRLAEELDAELAPVEEDRFPDGEQIVRVPPELDGTVVVVHSMSPPQDENLVKAIITLDAARENGAEE
VIAIVPYMAYSRQDRRFEPGEPVSFRAVARAVSANADALITVDLHEPGTLKYFDVPAENVSAAEELGKYLAERFEGEDLV
VIGPDEGARELAREVASICGVEYDHLEKKRLSGDEVEIHPKELDVEGRTVVLVDDMIDTGGTMVEAARALRDQGAGTLYA
ACTHALLTRNAATRLLASGFEDIIATDTVPNPFEKVSVAPPVAEAVENLSG
>Mature_291_residues
MIVLATSEGVKLGRRLAEELDAELAPVEEDRFPDGEQIVRVPPELDGTVVVVHSMSPPQDENLVKAIITLDAARENGAEE
VIAIVPYMAYSRQDRRFEPGEPVSFRAVARAVSANADALITVDLHEPGTLKYFDVPAENVSAAEELGKYLAERFEGEDLV
VIGPDEGARELAREVASICGVEYDHLEKKRLSGDEVEIHPKELDVEGRTVVLVDDMIDTGGTMVEAARALRDQGAGTLYA
ACTHALLTRNAATRLLASGFEDIIATDTVPNPFEKVSVAPPVAEAVENLSG

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family

Homologues:

Organism=Homo sapiens, GI4506129, Length=273, Percent_Identity=30.4029304029304, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI84875539, Length=276, Percent_Identity=30.7971014492754, Blast_Score=144, Evalue=9e-35,
Organism=Homo sapiens, GI4506127, Length=271, Percent_Identity=30.9963099630996, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI28557709, Length=271, Percent_Identity=30.2583025830258, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI4506133, Length=305, Percent_Identity=25.2459016393443, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1787458, Length=274, Percent_Identity=32.8467153284672, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17554704, Length=271, Percent_Identity=32.1033210332103, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25149168, Length=271, Percent_Identity=32.1033210332103, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17554702, Length=271, Percent_Identity=32.1033210332103, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71989924, Length=271, Percent_Identity=32.1033210332103, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17570245, Length=306, Percent_Identity=24.1830065359477, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6320946, Length=296, Percent_Identity=25.3378378378378, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321776, Length=275, Percent_Identity=28, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319403, Length=297, Percent_Identity=26.5993265993266, Blast_Score=115, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6322667, Length=195, Percent_Identity=25.6410256410256, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21355239, Length=262, Percent_Identity=32.0610687022901, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI45551540, Length=285, Percent_Identity=29.8245614035088, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24651458, Length=286, Percent_Identity=24.1258741258741, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24651456, Length=286, Percent_Identity=24.1258741258741, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI281362873, Length=286, Percent_Identity=24.1258741258741, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24651454, Length=286, Percent_Identity=24.1258741258741, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KPRS_METKA (Q8TUT6)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614950.1
- ProteinModelPortal:   Q8TUT6
- SMR:   Q8TUT6
- GeneID:   1478262
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1667
- NMPDR:   fig|190192.1.peg.1663
- HOGENOM:   HBG519284
- OMA:   FPDGEIY
- BioCyc:   MKAN190192:MK1667-MONOMER
- BRENDA:   2.7.6.1
- GO:   GO:0005737
- HAMAP:   MF_00583_A
- InterPro:   IPR005946
- InterPro:   IPR000836
- TIGRFAMs:   TIGR01251

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.7.6.1

Molecular weight: Translated: 31383; Mature: 31383

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: PS00114 PRPP_SYNTHASE; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVLATSEGVKLGRRLAEELDAELAPVEEDRFPDGEQIVRVPPELDGTVVVVHSMSPPQD
CEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEECCCCCCH
ENLVKAIITLDAARENGAEEVIAIVPYMAYSRQDRRFEPGEPVSFRAVARAVSANADALI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEE
TVDLHEPGTLKYFDVPAENVSAAEELGKYLAERFEGEDLVVIGPDEGARELAREVASICG
EEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHC
VEYDHLEKKRLSGDEVEIHPKELDVEGRTVVLVDDMIDTGGTMVEAARALRDQGAGTLYA
CCHHHHHHHHCCCCCEEECCCEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHH
ACTHALLTRNAATRLLASGFEDIIATDTVPNPFEKVSVAPPVAEAVENLSG
HHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MIVLATSEGVKLGRRLAEELDAELAPVEEDRFPDGEQIVRVPPELDGTVVVVHSMSPPQD
CEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEEECCCCCCH
ENLVKAIITLDAARENGAEEVIAIVPYMAYSRQDRRFEPGEPVSFRAVARAVSANADALI
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEE
TVDLHEPGTLKYFDVPAENVSAAEELGKYLAERFEGEDLVVIGPDEGARELAREVASICG
EEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHC
VEYDHLEKKRLSGDEVEIHPKELDVEGRTVVLVDDMIDTGGTMVEAARALRDQGAGTLYA
CCHHHHHHHHCCCCCEEECCCEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHH
ACTHALLTRNAATRLLASGFEDIIATDTVPNPFEKVSVAPPVAEAVENLSG
HHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014