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The map label for this gene is CarB_3

Identifier: 20095102

GI number: 20095102

Start: 1667891

End: 1669519

Strand: Direct

Name: CarB_3

Synonym: MK1666

Alternate gene names: 20095102

Gene position: 1667891-1669519 (Clockwise)

Preceding gene: 20095093

Following gene: 20095103

Centisome position: 98.4

GC content: 59.12

Gene sequence:

>1629_bases
ATGTCCGATAAAGTCCTCGTGATAGGTGCGGGCCCTAACCGCATCGGCCAGGGGATCGAATTCGATTACTGTACAGTTCA
CGCCGTGTGGGCGATTCAGGAGGAGGGTTACAAGGCGATCATAGTGAACAACAACCCCGAGACGGTCTCCACGGATTACG
ATACTTCGGATAAACTGTACTTCGAACCAATCACACTCGAAGACGTACTCAACATAGTCGAGAAGGAGCGGCCTATCGGC
GTGCTGACGCAGTTCGGGGGGCAGACCTCCGTCAACCTAACCGTGCCACTCGCGGAGCGTGGAGTCCGTGTTCTTGGTAC
CGATCCCGACGACGTCGACCGACTCGAGGATCGGGACAGGTTCTCAAAGCTCCTGAAGAAGCTCGGTATCCCGCAGCCCG
AGAGCGGGACCGCCAACGACCCCGAAGAAGCCGTTGAGGTAGCGGAAGATATCGGTTACCCAGTCCTTGTGCGTCCATCG
TATGTGATAGGTGGCCGAGCCATGGAGATAGTGTACGACGAGGAGGATCTCCGTAGGTACATCGAGGAAGCCGCCAAGGT
ATCCCCGGAGCACCCGATCTTGATAGACCGGTTCATCGAGGGCGGCATAGAGTGCGAGATCGACGGTGCGCGGGATGAGG
CCGGGAACGTGCTCATCCCCGGGATCATGGAACACATCGAGGAAGCCGGTGTGCACTCCGGTGATTCCGCCTGTGTCGTG
CCACCACAGACGCTCCCGGAGCACGCACAGGAGACGGTCCTCGAGTACGCCGAAGACATCGCCGAGGGGGCAAACGTGAT
CGGGCTTATTAACATTCAGTTCGTCTACGACCCTGAAGAGGACGAGGTGTACGTTATCGAAGCCAACCCGCGCGCCAGTC
GAACGGTGCCTTTCATCAGCAAGGCCGTCGGAATACCACTCGCCAAAATCGGAACCAAGGCCATCCTCGGCCGTGAGATC
CCCGAGGTCCTGGACGAGATGGGGCTAGAGCCTCCGGACGGTGACCCCGGAATCGTCGCCGTTAAAGAGGCCGTATTCTC
CTTCGAGAAGTGGCCCGGAGTGGATCCCGTGCTGGGCCCCGAGATGAAGGCTACGGGAGAAGTTATGGGGATCGACAGGA
CGTTCGGTGCCGCCTACTGGAAGGCTCAGCTCGCAGCAGGACACGAGCTTCCTCTCGAGGGTACCGCCGTCATATCGGTA
GCCGACCGGGACAAGCCCGATATCGTCCCGATCGCTCGAAAGCTTCAGCGGTTAGGGTTCGATCTGCTCGCGACGCGCGG
CACCGCATCGCACCTGCGCGAACACGGGATCGAGTGCGAGGTGGTGAGGAAAGTATCGGAGGGTAGTCCCAACATAGTTG
ATCTCATCCGTGAAGGTGAAATCGACCTGATTATCAACACCCCAACGGAAGGGAAGGACGCTCGCCGCGACGGGTACGCC
ATCCGTCGGGCAGCTGTGAAGTTCAAGGTCCCGTACATCACTACGATCGCGGCGGCAAAGGCGGCCGTTGAGGCGATAGA
ACTGGTGAAAGAGAAAGGAGTCACCGTCAACTGCCTCCACGACATCCACAAGGGTGATTGGACACCTCGCGAGGTGAAAC
CCGAAGAGTTGACTCGCTACGGAGGATGA

Upstream 100 bases:

>100_bases
TCCGCGGCTTTCCTGGCCGCGTTCCGTTTGCCGCTCAAAGCCGAACCCCCGAGGGTTTCTTAATGCGGCGTGTGGGCCCT
CACACGCGGGTAGCCGGACG

Downstream 100 bases:

>100_bases
TGGGGGAGAGACGTGTTGATAGTCCTCGCCACATCGGAGGGTGTCAAGCTGGGTCGGCGTCTCGCAGAGGAGCTCGACGC
GGAGCTGGCGCCGGTCGAGG

Product: carbamoylphosphate synthase large subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MSDKVLVIGAGPNRIGQGIEFDYCTVHAVWAIQEEGYKAIIVNNNPETVSTDYDTSDKLYFEPITLEDVLNIVEKERPIG
VLTQFGGQTSVNLTVPLAERGVRVLGTDPDDVDRLEDRDRFSKLLKKLGIPQPESGTANDPEEAVEVAEDIGYPVLVRPS
YVIGGRAMEIVYDEEDLRRYIEEAAKVSPEHPILIDRFIEGGIECEIDGARDEAGNVLIPGIMEHIEEAGVHSGDSACVV
PPQTLPEHAQETVLEYAEDIAEGANVIGLINIQFVYDPEEDEVYVIEANPRASRTVPFISKAVGIPLAKIGTKAILGREI
PEVLDEMGLEPPDGDPGIVAVKEAVFSFEKWPGVDPVLGPEMKATGEVMGIDRTFGAAYWKAQLAAGHELPLEGTAVISV
ADRDKPDIVPIARKLQRLGFDLLATRGTASHLREHGIECEVVRKVSEGSPNIVDLIREGEIDLIINTPTEGKDARRDGYA
IRRAAVKFKVPYITTIAAAKAAVEAIELVKEKGVTVNCLHDIHKGDWTPREVKPEELTRYGG

Sequences:

>Translated_542_residues
MSDKVLVIGAGPNRIGQGIEFDYCTVHAVWAIQEEGYKAIIVNNNPETVSTDYDTSDKLYFEPITLEDVLNIVEKERPIG
VLTQFGGQTSVNLTVPLAERGVRVLGTDPDDVDRLEDRDRFSKLLKKLGIPQPESGTANDPEEAVEVAEDIGYPVLVRPS
YVIGGRAMEIVYDEEDLRRYIEEAAKVSPEHPILIDRFIEGGIECEIDGARDEAGNVLIPGIMEHIEEAGVHSGDSACVV
PPQTLPEHAQETVLEYAEDIAEGANVIGLINIQFVYDPEEDEVYVIEANPRASRTVPFISKAVGIPLAKIGTKAILGREI
PEVLDEMGLEPPDGDPGIVAVKEAVFSFEKWPGVDPVLGPEMKATGEVMGIDRTFGAAYWKAQLAAGHELPLEGTAVISV
ADRDKPDIVPIARKLQRLGFDLLATRGTASHLREHGIECEVVRKVSEGSPNIVDLIREGEIDLIINTPTEGKDARRDGYA
IRRAAVKFKVPYITTIAAAKAAVEAIELVKEKGVTVNCLHDIHKGDWTPREVKPEELTRYGG
>Mature_541_residues
SDKVLVIGAGPNRIGQGIEFDYCTVHAVWAIQEEGYKAIIVNNNPETVSTDYDTSDKLYFEPITLEDVLNIVEKERPIGV
LTQFGGQTSVNLTVPLAERGVRVLGTDPDDVDRLEDRDRFSKLLKKLGIPQPESGTANDPEEAVEVAEDIGYPVLVRPSY
VIGGRAMEIVYDEEDLRRYIEEAAKVSPEHPILIDRFIEGGIECEIDGARDEAGNVLIPGIMEHIEEAGVHSGDSACVVP
PQTLPEHAQETVLEYAEDIAEGANVIGLINIQFVYDPEEDEVYVIEANPRASRTVPFISKAVGIPLAKIGTKAILGREIP
EVLDEMGLEPPDGDPGIVAVKEAVFSFEKWPGVDPVLGPEMKATGEVMGIDRTFGAAYWKAQLAAGHELPLEGTAVISVA
DRDKPDIVPIARKLQRLGFDLLATRGTASHLREHGIECEVVRKVSEGSPNIVDLIREGEIDLIINTPTEGKDARRDGYAI
RRAAVKFKVPYITTIAAAKAAVEAIELVKEKGVTVNCLHDIHKGDWTPREVKPEELTRYGG

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI170295797, Length=512, Percent_Identity=38.8671875, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI169790915, Length=512, Percent_Identity=38.8671875, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI21361331, Length=512, Percent_Identity=38.8671875, Blast_Score=364, Evalue=1e-100,
Organism=Homo sapiens, GI18105007, Length=516, Percent_Identity=39.1472868217054, Blast_Score=363, Evalue=1e-100,
Organism=Escherichia coli, GI1786216, Length=519, Percent_Identity=51.252408477842, Blast_Score=509, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI193204318, Length=540, Percent_Identity=35.3703703703704, Blast_Score=343, Evalue=2e-94,
Organism=Saccharomyces cerevisiae, GI6322331, Length=517, Percent_Identity=41.5860735009671, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6322569, Length=495, Percent_Identity=36.5656565656566, Blast_Score=335, Evalue=1e-92,
Organism=Saccharomyces cerevisiae, GI6319685, Length=298, Percent_Identity=21.1409395973154, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24642586, Length=521, Percent_Identity=35.5086372360845, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI45555749, Length=443, Percent_Identity=37.020316027088, Blast_Score=308, Evalue=8e-84,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARB2_METKA (Q8TUT7)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614949.1
- ProteinModelPortal:   Q8TUT7
- GeneID:   1478261
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1666
- NMPDR:   fig|190192.1.peg.1662
- HOGENOM:   HBG405439
- OMA:   CVQAVFA
- BioCyc:   MKAN190192:MK1666-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01210_A
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR011607
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.1380
- Gene3D:   G3DSA:3.40.50.20
- PRINTS:   PR00098
- SMART:   SM00851

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02142 MGS; SSF52335 MGS-like_dom; SSF52440 PreATP-grasp-like

EC number: =6.3.5.5

Molecular weight: Translated: 59226; Mature: 59095

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS50975 ATP_GRASP; PS00866 CPSASE_1; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKVLVIGAGPNRIGQGIEFDYCTVHAVWAIQEEGYKAIIVNNNPETVSTDYDTSDKLY
CCCCEEEEECCCHHHCCCCCCCEEEEEEEEEEECCCCEEEEECCCCCEEECCCCCCCCEE
FEPITLEDVLNIVEKERPIGVLTQFGGQTSVNLTVPLAERGVRVLGTDPDDVDRLEDRDR
EECCCHHHHHHHHHCCCCCCCEECCCCCEEEEEEEEHHHCCEEEECCCCHHHHHHHHHHH
FSKLLKKLGIPQPESGTANDPEEAVEVAEDIGYPVLVRPSYVIGGRAMEIVYDEEDLRRY
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEECCEEEEEEECHHHHHHH
IEEAAKVSPEHPILIDRFIEGGIECEIDGARDEAGNVLIPGIMEHIEEAGVHSGDSACVV
HHHHHHCCCCCCEEHHHHHCCCCEEEECCCCCCCCCEECHHHHHHHHHCCCCCCCCEEEE
PPQTLPEHAQETVLEYAEDIAEGANVIGLINIQFVYDPEEDEVYVIEANPRASRTVPFIS
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHH
KAVGIPLAKIGTKAILGREIPEVLDEMGLEPPDGDPGIVAVKEAVFSFEKWPGVDPVLGP
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCC
EMKATGEVMGIDRTFGAAYWKAQLAAGHELPLEGTAVISVADRDKPDIVPIARKLQRLGF
CCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCH
DLLATRGTASHLREHGIECEVVRKVSEGSPNIVDLIREGEIDLIINTPTEGKDARRDGYA
HHEECCCCHHHHHHCCCCEEEHHHHHCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCHH
IRRAAVKFKVPYITTIAAAKAAVEAIELVKEKGVTVNCLHDIHKGDWTPREVKPEELTRY
HHHEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCHHHHHHC
GG
CC
>Mature Secondary Structure 
SDKVLVIGAGPNRIGQGIEFDYCTVHAVWAIQEEGYKAIIVNNNPETVSTDYDTSDKLY
CCCEEEEECCCHHHCCCCCCCEEEEEEEEEEECCCCEEEEECCCCCEEECCCCCCCCEE
FEPITLEDVLNIVEKERPIGVLTQFGGQTSVNLTVPLAERGVRVLGTDPDDVDRLEDRDR
EECCCHHHHHHHHHCCCCCCCEECCCCCEEEEEEEEHHHCCEEEECCCCHHHHHHHHHHH
FSKLLKKLGIPQPESGTANDPEEAVEVAEDIGYPVLVRPSYVIGGRAMEIVYDEEDLRRY
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECCCEEECCEEEEEEECHHHHHHH
IEEAAKVSPEHPILIDRFIEGGIECEIDGARDEAGNVLIPGIMEHIEEAGVHSGDSACVV
HHHHHHCCCCCCEEHHHHHCCCCEEEECCCCCCCCCEECHHHHHHHHHCCCCCCCCEEEE
PPQTLPEHAQETVLEYAEDIAEGANVIGLINIQFVYDPEEDEVYVIEANPRASRTVPFIS
CCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHH
KAVGIPLAKIGTKAILGREIPEVLDEMGLEPPDGDPGIVAVKEAVFSFEKWPGVDPVLGP
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCC
EMKATGEVMGIDRTFGAAYWKAQLAAGHELPLEGTAVISVADRDKPDIVPIARKLQRLGF
CCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCH
DLLATRGTASHLREHGIECEVVRKVSEGSPNIVDLIREGEIDLIINTPTEGKDARRDGYA
HHEECCCCHHHHHHCCCCEEEHHHHHCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCHH
IRRAAVKFKVPYITTIAAAKAAVEAIELVKEKGVTVNCLHDIHKGDWTPREVKPEELTRY
HHHEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCCCCCCCCHHHHHHC
GG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014