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The map label for this gene is thrA [C]

Identifier: 20094971

GI number: 20094971

Start: 1551496

End: 1552785

Strand: Reverse

Name: thrA [C]

Synonym: MK1535

Alternate gene names: 20094971

Gene position: 1552785-1551496 (Counterclockwise)

Preceding gene: 20094972

Following gene: 20094969

Centisome position: 91.61

GC content: 65.12

Gene sequence:

>1290_bases
TTGGAGGTTCACAAGTTCGGAGGCACGTCGGTAGCCTCTGAGGAGGGACTTCGGACCCTCGAAGCTTCCGCGGCTTCCGG
CCACGTGATCGTGGTCTCGGCCCTAGCCGGCGTCACAGATGCACTGGAGGACTTCGTTCGACGCGCCGCCGAAGGTTCCG
CCGACCCGACCCCCATACTAGAGCGTCACAGGGAGTTCATCACGGAACATCTGGAACAGCGGCACGAGGAGGTAGAATCG
TTCCTGAAAGACATGGAGACACTCCTCCACGGTGTCGCCACGGTTCTCGAGCAACTTGGCAGACCCGAAGAGCGCCTCCG
CGACTTGGTTCTGTCCCTCGGAGAGCGCGCGTCCGCCCGCATCGTGGCGGCGTACCTGAAGGAGCGGGGTATCAAGGCGA
TGGCGTACGACGCCTGGGACGTGGGACTGGTCACCACCGACGACCCCGGAAACGCCGACATCGTCGGATGGGACGGAACG
CGCTCGAGACTCCTACGAGACCTCCGCTCGGGTCGCGTTCCGGTCGTCACGGGGTTCATCGGGAGGTCAGACCGCGGCCA
CGTGACCACGCTGGGACGAGGGGGCTCCGACTACACGGCCACCGTCCTGGCCGGGGTGTTGGGTTCGCGGTCAGTAATAT
GGACGGACGTCGACGGCATCATGACCACCGACCCGGAACTGGCGGACGCGGAGGTCGTCGAGCGGCTGTCGTACGAGGAA
GCTATGATGGCCGGTGCATCGGGGGCGGGGGTGATCCACCCTAAGGCCGTGGAGGCCGCTAAGAACCTCGGAGTGACAGT
CCTGATCGGGAACTCGTTCACCGGGGAAATAGGGACCGTGATATCCGACAGTACGGAGCCCGGCCCTAAGGTAGTGGCGT
CCCGGGACGACGTCGCGCTGATTAGGGTGAGCGGTGCCAAAATGGTGGACGAGCCCGGAGTGGTGGGTAGGGTGACCTCC
GCGCTGGGGAACGCCGGCGTGAACCTCCTCGCGGTGTTCACGACGGTCTCGGAGCCTTACATCAACCTCCTGGTAGAGGA
GACCGCCCTGAGGAGCGCGGAGGAAGCCCTGAACGGCCTGGACTACGACTGGGAGGTCGACAAGGACGTGGGCTTGGTCA
CGGTGGTGGGCGAAGGGATGTCGGCCCGGGACGTGTCCACGTTCTTGGCAGCCTGCGAGGGGTTCGACCTGCTAGGTAGC
GCACATGGGGTCGTAGCGGTGAGCGTCGTGGTACCGGAGTCCGAGGTCCGCGAGGTCACGCGGCGACTGGCGGAACGGTT
GCTGGCGTAA

Upstream 100 bases:

>100_bases
TGGAGATCCCGTGGCCCGTATGGGCCGCCCGGGTTCAAATCCCGGCCCCGGCGCCAAACCCAGAATCGTTACGAATCCCA
TAACGAAGAGGGGACCGAGC

Downstream 100 bases:

>100_bases
CCCACGTAGCCCGCGGTCTGGGACCCGTCGGTCGGATCCTCCACACCAGGAGGAACAGTAGTGTCAGGAGCAGGGCGGCG
ACCCATTCCCAGATCATCTT

Product: aspartokinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MEVHKFGGTSVASEEGLRTLEASAASGHVIVVSALAGVTDALEDFVRRAAEGSADPTPILERHREFITEHLEQRHEEVES
FLKDMETLLHGVATVLEQLGRPEERLRDLVLSLGERASARIVAAYLKERGIKAMAYDAWDVGLVTTDDPGNADIVGWDGT
RSRLLRDLRSGRVPVVTGFIGRSDRGHVTTLGRGGSDYTATVLAGVLGSRSVIWTDVDGIMTTDPELADAEVVERLSYEE
AMMAGASGAGVIHPKAVEAAKNLGVTVLIGNSFTGEIGTVISDSTEPGPKVVASRDDVALIRVSGAKMVDEPGVVGRVTS
ALGNAGVNLLAVFTTVSEPYINLLVEETALRSAEEALNGLDYDWEVDKDVGLVTVVGEGMSARDVSTFLAACEGFDLLGS
AHGVVAVSVVVPESEVREVTRRLAERLLA

Sequences:

>Translated_429_residues
MEVHKFGGTSVASEEGLRTLEASAASGHVIVVSALAGVTDALEDFVRRAAEGSADPTPILERHREFITEHLEQRHEEVES
FLKDMETLLHGVATVLEQLGRPEERLRDLVLSLGERASARIVAAYLKERGIKAMAYDAWDVGLVTTDDPGNADIVGWDGT
RSRLLRDLRSGRVPVVTGFIGRSDRGHVTTLGRGGSDYTATVLAGVLGSRSVIWTDVDGIMTTDPELADAEVVERLSYEE
AMMAGASGAGVIHPKAVEAAKNLGVTVLIGNSFTGEIGTVISDSTEPGPKVVASRDDVALIRVSGAKMVDEPGVVGRVTS
ALGNAGVNLLAVFTTVSEPYINLLVEETALRSAEEALNGLDYDWEVDKDVGLVTVVGEGMSARDVSTFLAACEGFDLLGS
AHGVVAVSVVVPESEVREVTRRLAERLLA
>Mature_429_residues
MEVHKFGGTSVASEEGLRTLEASAASGHVIVVSALAGVTDALEDFVRRAAEGSADPTPILERHREFITEHLEQRHEEVES
FLKDMETLLHGVATVLEQLGRPEERLRDLVLSLGERASARIVAAYLKERGIKAMAYDAWDVGLVTTDDPGNADIVGWDGT
RSRLLRDLRSGRVPVVTGFIGRSDRGHVTTLGRGGSDYTATVLAGVLGSRSVIWTDVDGIMTTDPELADAEVVERLSYEE
AMMAGASGAGVIHPKAVEAAKNLGVTVLIGNSFTGEIGTVISDSTEPGPKVVASRDDVALIRVSGAKMVDEPGVVGRVTS
ALGNAGVNLLAVFTTVSEPYINLLVEETALRSAEEALNGLDYDWEVDKDVGLVTVVGEGMSARDVSTFLAACEGFDLLGS
AHGVVAVSVVVPESEVREVTRRLAERLLA

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=426, Percent_Identity=29.81220657277, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790455, Length=349, Percent_Identity=31.5186246418338, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790376, Length=410, Percent_Identity=26.8292682926829, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6320893, Length=377, Percent_Identity=24.9336870026525, Blast_Score=83, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 45496; Mature: 45496

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVHKFGGTSVASEEGLRTLEASAASGHVIVVSALAGVTDALEDFVRRAAEGSADPTPIL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHH
ERHREFITEHLEQRHEEVESFLKDMETLLHGVATVLEQLGRPEERLRDLVLSLGERASAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHH
IVAAYLKERGIKAMAYDAWDVGLVTTDDPGNADIVGWDGTRSRLLRDLRSGRVPVVTGFI
HHHHHHHHCCCEEEEECCEEEEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCEEEEEEC
GRSDRGHVTTLGRGGSDYTATVLAGVLGSRSVIWTDVDGIMTTDPELADAEVVERLSYEE
CCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCEECCCCCHHHHHHHHHHHHH
AMMAGASGAGVIHPKAVEAAKNLGVTVLIGNSFTGEIGTVISDSTEPGPKVVASRDDVAL
HHHCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCHHCCCCCCCCCCEEECCCCEEE
IRVSGAKMVDEPGVVGRVTSALGNAGVNLLAVFTTVSEPYINLLVEETALRSAEEALNGL
EEECCCCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
DYDWEVDKDVGLVTVVGEGMSARDVSTFLAACEGFDLLGSAHGVVAVSVVVPESEVREVT
CCCEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHCCCCCEEEEEEECCHHHHHHHH
RRLAERLLA
HHHHHHHCC
>Mature Secondary Structure
MEVHKFGGTSVASEEGLRTLEASAASGHVIVVSALAGVTDALEDFVRRAAEGSADPTPIL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCHHH
ERHREFITEHLEQRHEEVESFLKDMETLLHGVATVLEQLGRPEERLRDLVLSLGERASAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHH
IVAAYLKERGIKAMAYDAWDVGLVTTDDPGNADIVGWDGTRSRLLRDLRSGRVPVVTGFI
HHHHHHHHCCCEEEEECCEEEEEEEECCCCCCEEEECCCHHHHHHHHHHCCCCEEEEEEC
GRSDRGHVTTLGRGGSDYTATVLAGVLGSRSVIWTDVDGIMTTDPELADAEVVERLSYEE
CCCCCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCEECCCCCHHHHHHHHHHHHH
AMMAGASGAGVIHPKAVEAAKNLGVTVLIGNSFTGEIGTVISDSTEPGPKVVASRDDVAL
HHHCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCHHCCCCCCCCCCEEECCCCEEE
IRVSGAKMVDEPGVVGRVTSALGNAGVNLLAVFTTVSEPYINLLVEETALRSAEEALNGL
EEECCCCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
DYDWEVDKDVGLVTVVGEGMSARDVSTFLAACEGFDLLGSAHGVVAVSVVVPESEVREVT
CCCEEECCCCCEEEEECCCCCHHHHHHHHHHHCCCHHHCCCCCEEEEEEECCHHHHHHHH
RRLAERLLA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]