Click here to switch to the map view.

The map label for this gene is PyrI

Identifier: 20094917

GI number: 20094917

Start: 1502086

End: 1502556

Strand: Direct

Name: PyrI

Synonym: MK1481

Alternate gene names: 20094917

Gene position: 1502086-1502556 (Clockwise)

Preceding gene: 20094916

Following gene: 20094918

Centisome position: 88.62

GC content: 58.39

Gene sequence:

>471_bases
GTGATCGTGGCCCTCAAAGTGAAGAGAATCGAAATGGGTACCGTCCTCGACCACCTGCCGCCGGGTACCGCCCCCCAGAT
TATGCGTATCCTGGACATCGATCCGACCGAGACTACCCTGCTCGTCGCCATAAACGTGGAGAGCTCGAAGATGGGTCGCA
AGGACATACTGAAGATCGAAGGGAAGATCCTGAGCGAGGAGGAGGCGAACAAGGTCGCGCTCGTGGCCCCTAACGCCACA
GTCAACATCGTTCGAGACTATTCCGTGGCCGAGAAGTTCCAGGTCAAACCACCCGAGAGGGTGGAAGGGTTCCTCCGCTG
CCCCAACCCCAACTGCATCACGAACGACGAAAGGGAACCTGTGGACACTGTCTTCGTTAGGGAGTCCAAAAAGCCGCTGG
AGTACCGCTGTCGGTACTGCGAGCGTACCGTCCGGGAGGATCAGATCCGTGAGCTGATACGCCCTAGCTAG

Upstream 100 bases:

>100_bases
AGCCCGATGTGGACGAACTACCGCAGGCCCGCTACTTCGATCAGGTTCGTAACGGTGTGATCGTACGGATGGCACTCCTC
GACCTCATCCTCGGGGGCGG

Downstream 100 bases:

>100_bases
TCTCCGGGACCAGTTAATGCGATAAAGACGAGGGGGTGAGTGAAAGTCGGGATACCCGCCATCATACAACCTGATGGGTA
GGTGATACATTGTCCGATCC

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MIVALKVKRIEMGTVLDHLPPGTAPQIMRILDIDPTETTLLVAINVESSKMGRKDILKIEGKILSEEEANKVALVAPNAT
VNIVRDYSVAEKFQVKPPERVEGFLRCPNPNCITNDEREPVDTVFVRESKKPLEYRCRYCERTVREDQIRELIRPS

Sequences:

>Translated_156_residues
MIVALKVKRIEMGTVLDHLPPGTAPQIMRILDIDPTETTLLVAINVESSKMGRKDILKIEGKILSEEEANKVALVAPNAT
VNIVRDYSVAEKFQVKPPERVEGFLRCPNPNCITNDEREPVDTVFVRESKKPLEYRCRYCERTVREDQIRELIRPS
>Mature_156_residues
MIVALKVKRIEMGTVLDHLPPGTAPQIMRILDIDPTETTLLVAINVESSKMGRKDILKIEGKILSEEEANKVALVAPNAT
VNIVRDYSVAEKFQVKPPERVEGFLRCPNPNCITNDEREPVDTVFVRESKKPLEYRCRYCERTVREDQIRELIRPS

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=139, Percent_Identity=38.8489208633094, Blast_Score=113, Evalue=6e-27,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_METKA (Q8TVB1)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614764.1
- ProteinModelPortal:   Q8TVB1
- SMR:   Q8TVB1
- GeneID:   1478076
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1481
- NMPDR:   fig|190192.1.peg.1477
- HOGENOM:   HBG305327
- OMA:   EGTVIDH
- ProtClustDB:   PRK00893
- BioCyc:   MKAN190192:MK1481-MONOMER
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: 2.1.3.2

Molecular weight: Translated: 17786; Mature: 17786

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVALKVKRIEMGTVLDHLPPGTAPQIMRILDIDPTETTLLVAINVESSKMGRKDILKIE
CEEEEEEEEEEHHHHHHHCCCCCHHHEEEEEECCCCCEEEEEEEECCCCCCCCHHEEEEC
GKILSEEEANKVALVAPNATVNIVRDYSVAEKFQVKPPERVEGFLRCPNPNCITNDEREP
CEEECCCCCCEEEEECCCCEEEEEECCCCHHHCCCCCHHHHHHEEECCCCCCCCCCCCCC
VDTVFVRESKKPLEYRCRYCERTVREDQIRELIRPS
CEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MIVALKVKRIEMGTVLDHLPPGTAPQIMRILDIDPTETTLLVAINVESSKMGRKDILKIE
CEEEEEEEEEEHHHHHHHCCCCCHHHEEEEEECCCCCEEEEEEEECCCCCCCCHHEEEEC
GKILSEEEANKVALVAPNATVNIVRDYSVAEKFQVKPPERVEGFLRCPNPNCITNDEREP
CEEECCCCCCEEEEECCCCEEEEEECCCCHHHCCCCCHHHHHHEEECCCCCCCCCCCCCC
VDTVFVRESKKPLEYRCRYCERTVREDQIRELIRPS
CEEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014