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The map label for this gene is pyrB

Identifier: 20094916

GI number: 20094916

Start: 1501160

End: 1502089

Strand: Direct

Name: pyrB

Synonym: MK1480

Alternate gene names: 20094916

Gene position: 1501160-1502089 (Clockwise)

Preceding gene: 20094915

Following gene: 20094917

Centisome position: 88.57

GC content: 59.57

Gene sequence:

>930_bases
GTGTCGTCCTTCGCGAACAGGGACGTAATCTCCGTCCGGGATTTCACCCGGAAAGAGCTGGAGGAGCTACTAAGTCACGC
GGAAGAGATGGAACGCGTGTACGAACGCGGCGGAGACGACAGACTTTCCGGCAAGATCCTAGCCACTCTCTTCTTCTCTC
CTAGCACGAGGACCCGACTCTCGTTCGAAAGTGCCATGCACCGACTCGGCGGTGACGTGATCTCATTGGGCGGCAAAGAG
GCGGCATCGACGGCTAAGGGTGAAAATCTCGCGGACACCGTCCGCACCGTCGAGCACTACTGCGACGTTATCGTGTTGCG
GCACCCGAAGGAGGGTGCCGCCCGGCTCGCCGCGGAACTTACCGACGTCCCCGTGATCAACGCGGGCGACGGCGCCAACC
AACACCCGACGCAGACGTTCCTAGACCTCTATACGATCATGAAGGAGAAGGGACGTATCGGAGGACTCAGAATCGGGCTG
TTGGGAGACCTCAAATACGGACGAACCGTCCACTCGCTGGCGTACGCTCTCGCACTGTTCGGCGCGAAAATCCACCTGAT
ATCACCGGAGGAACTCCGGATGCCCTCTCACATATTGGAAGAGCTCGAGCAGATAGGCGCGGAGGTCGAGGAACACCGAG
ACCTAGAGGAGATCCTACCCGACTTGGACGTACTCTACGTGACCAGGATTCAGCGCGAGATGTTTCCGGACCCGGAGGAG
TTCGAGCGCGTGAAAGGTAGCTACAAGGTGACGAGGGAACTCATCGAAGAACACGCGCGGAGCGATCTGGTAATCCTGCA
CCCACTCCCCAGGGTGGATGAGATCGAGCCCGATGTGGACGAACTACCGCAGGCCCGCTACTTCGATCAGGTTCGTAACG
GTGTGATCGTACGGATGGCACTCCTCGACCTCATCCTCGGGGGCGGGTGA

Upstream 100 bases:

>100_bases
CACCTCCGATGACCTCCCGCCCTCCAGCCGGGGGCACAATCGTTACTTCTTCAACCGCGAGCCGTTTAAGGTTCCATACA
CGTCGCGTTGGAGGGCCTCC

Downstream 100 bases:

>100_bases
TCGTGGCCCTCAAAGTGAAGAGAATCGAAATGGGTACCGTCCTCGACCACCTGCCGCCGGGTACCGCCCCCCAGATTATG
CGTATCCTGGACATCGATCC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSSFANRDVISVRDFTRKELEELLSHAEEMERVYERGGDDRLSGKILATLFFSPSTRTRLSFESAMHRLGGDVISLGGKE
AASTAKGENLADTVRTVEHYCDVIVLRHPKEGAARLAAELTDVPVINAGDGANQHPTQTFLDLYTIMKEKGRIGGLRIGL
LGDLKYGRTVHSLAYALALFGAKIHLISPEELRMPSHILEELEQIGAEVEEHRDLEEILPDLDVLYVTRIQREMFPDPEE
FERVKGSYKVTRELIEEHARSDLVILHPLPRVDEIEPDVDELPQARYFDQVRNGVIVRMALLDLILGGG

Sequences:

>Translated_309_residues
MSSFANRDVISVRDFTRKELEELLSHAEEMERVYERGGDDRLSGKILATLFFSPSTRTRLSFESAMHRLGGDVISLGGKE
AASTAKGENLADTVRTVEHYCDVIVLRHPKEGAARLAAELTDVPVINAGDGANQHPTQTFLDLYTIMKEKGRIGGLRIGL
LGDLKYGRTVHSLAYALALFGAKIHLISPEELRMPSHILEELEQIGAEVEEHRDLEEILPDLDVLYVTRIQREMFPDPEE
FERVKGSYKVTRELIEEHARSDLVILHPLPRVDEIEPDVDELPQARYFDQVRNGVIVRMALLDLILGGG
>Mature_308_residues
SSFANRDVISVRDFTRKELEELLSHAEEMERVYERGGDDRLSGKILATLFFSPSTRTRLSFESAMHRLGGDVISLGGKEA
ASTAKGENLADTVRTVEHYCDVIVLRHPKEGAARLAAELTDVPVINAGDGANQHPTQTFLDLYTIMKEKGRIGGLRIGLL
GDLKYGRTVHSLAYALALFGAKIHLISPEELRMPSHILEELEQIGAEVEEHRDLEEILPDLDVLYVTRIQREMFPDPEEF
ERVKGSYKVTRELIEEHARSDLVILHPLPRVDEIEPDVDELPQARYFDQVRNGVIVRMALLDLILGGG

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=308, Percent_Identity=43.5064935064935, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI38788445, Length=305, Percent_Identity=26.5573770491803, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI2367364, Length=306, Percent_Identity=47.3856209150327, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1790703, Length=280, Percent_Identity=27.8571428571429, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786469, Length=280, Percent_Identity=26.4285714285714, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=321, Percent_Identity=43.613707165109, Blast_Score=237, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6322331, Length=306, Percent_Identity=43.4640522875817, Blast_Score=260, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6322373, Length=325, Percent_Identity=24.3076923076923, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24642586, Length=309, Percent_Identity=43.042071197411, Blast_Score=258, Evalue=5e-69,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_METKA (Q8TVB2)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614763.1
- ProteinModelPortal:   Q8TVB2
- SMR:   Q8TVB2
- GeneID:   1478075
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1480
- NMPDR:   fig|190192.1.peg.1476
- HOGENOM:   HBG579429
- OMA:   FKQSFYG
- ProtClustDB:   PRK00856
- BioCyc:   MKAN190192:MK1480-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34691; Mature: 34560

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFANRDVISVRDFTRKELEELLSHAEEMERVYERGGDDRLSGKILATLFFSPSTRTRL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHH
SFESAMHRLGGDVISLGGKEAASTAKGENLADTVRTVEHYCDVIVLRHPKEGAARLAAEL
HHHHHHHHHCCCEEECCCCHHHHHCCCCHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHH
TDVPVINAGDGANQHPTQTFLDLYTIMKEKGRIGGLRIGLLGDLKYGRTVHSLAYALALF
HCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
GAKIHLISPEELRMPSHILEELEQIGAEVEEHRDLEEILPDLDVLYVTRIQREMFPDPEE
CCEEEEECHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCHHH
FERVKGSYKVTRELIEEHARSDLVILHPLPRVDEIEPDVDELPQARYFDQVRNGVIVRMA
HHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCHHHHHHHHHCCHHHHHH
LLDLILGGG
HHHHHHCCC
>Mature Secondary Structure 
SSFANRDVISVRDFTRKELEELLSHAEEMERVYERGGDDRLSGKILATLFFSPSTRTRL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHH
SFESAMHRLGGDVISLGGKEAASTAKGENLADTVRTVEHYCDVIVLRHPKEGAARLAAEL
HHHHHHHHHCCCEEECCCCHHHHHCCCCHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHH
TDVPVINAGDGANQHPTQTFLDLYTIMKEKGRIGGLRIGLLGDLKYGRTVHSLAYALALF
HCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
GAKIHLISPEELRMPSHILEELEQIGAEVEEHRDLEEILPDLDVLYVTRIQREMFPDPEE
CCEEEEECHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCHHH
FERVKGSYKVTRELIEEHARSDLVILHPLPRVDEIEPDVDELPQARYFDQVRNGVIVRMA
HHHHCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHCCHHHHHHHHHCCHHHHHH
LLDLILGGG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014