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The map label for this gene is CobQ

Identifier: 20094850

GI number: 20094850

Start: 1442838

End: 1444322

Strand: Direct

Name: CobQ

Synonym: MK1414

Alternate gene names: 20094850

Gene position: 1442838-1444322 (Clockwise)

Preceding gene: 20094848

Following gene: 20094851

Centisome position: 85.12

GC content: 63.5

Gene sequence:

>1485_bases
TTGTCGGCGATCATGTTCGTCGGTACGGCCTCGAACTCCGGTAAGTCGTTCTTGGCGGCGGTTACATGCGCCTACCTGCG
TCAGCGTGGCGTAGACGTGGCTCCGTTCAAATCCCAGAACATGTCGCTGAACTCGTGTGTGGCCAAGGAGAACGGAGAAA
TCGCCGTCGCCCAGGCGTTCCAGGCCGCGATGGCCGGTCAGGAACCATCGATTCACCATAACCCCGTGCTCCTCAAGCCC
AAGGGGGAGCTCCGCTCGGAAGTCATCGTCCACGGGAAGCCCATCGGGACGATGTCGTACCGCGAGTACCGGGAGATCGT
CTTCGAAGATCCCTGGCAGGCCGTCTTGGAATCCGCGGAAATCCTCTCGGAGGAGCACGAGGTCATCGTCGCCGAGGGGG
CGGGGTCACCCGCGGAGATCAACGTCCTGGACACCGATATCGCGAACCTTCGGGTGGCCGAAGCCCTGGGCGCGGACGTG
ATCCTGGTCGCGGACATCAGTAGGGGTGGAGCCTTCGCGGCCGTCTACGGCACGATCGAGCTCTTACCGGAGCGATGGCG
ACGACTGATCAAGGGCTTCCTGTTCAACAAGTTCCTCGGCGATGAGTCCCTCCTCGAGCCGGGGATCAAGGAGCTGGAGC
GCCGCTTGGGCGTACGTTACCTCGGCACAGTGCGTCACGTCGGGGACTTCTGGATGCCTTGGGAAGACTCGGAGGCACTC
GACACCCACTCCCCCGGGCGCGGTTCCGTACGGATCGCGGTCATCAGGTTGCCCCGAATTTCCAACTTCACCGACTTCGA
GCCCCTCGCGATGGAGCCGGACGTGAGGGTGGAGTTCGTGGACCCCAGGGACAACCTCCCGGAGGATGCGGACGCGGTGA
TCCTCCCCGGCACCCGCACCACCATCTCCGACCTCGAAGAACTTCGAAAACGCGGGATGGACGAGGAGGTAGTTCAGGCG
GCCGATGAGGGTACTGTGGTGCTGGGGGTGTGCGGCGGGTACCAGATGCTCGGCAGGGAGCTCGTCGACGAGTCGGGGGG
CGAGCTCGACCCGGGGGAGTCGGTCCCGGGGCTCGGACTTCTCGACGCCGTGACCGTGTTCCCGAGCGACGCCGGCAAGG
TGACGGTGCGATCGGAGGGAGTCGTGAACCATCCGCACCTCCGCGGCATCCGCGTGGAGGGGTTCGAGATCCATGAGGGA
CGGACGTACACCGACGAACCCCACTTGGTCCGTTTACGATCAGGGTACGGAAACAGAGGCTGTTTTCTCGATGGAGCATA
CCGCACCGATCGGCCTGTTCTCGGGACCTACTTACACGGGATATTCTTCAACCGGCGCTTCCGCCACGAGTTCCTCAGAT
GGGTATCCGGGGGTAGGTGGAAGCCCCCCGAGCGGGACGTAGTTCGAGAGGCGGTGAAGCGGAACCTTCAGGTCGCCCTA
GAGATCGTCGAATCGACGGATCTTCCGGAGCTCCTGGGGGAATAA

Upstream 100 bases:

>100_bases
AAGGACGTGCGGACGGGGTACCCGGCCGCCCGTGCCAGTGCGTACGAAGCCGCCACATGGAACACGGGGTCCAAGGGAAC
TCCCCCGAGGGGTTCGTGAC

Downstream 100 bases:

>100_bases
GGGTGGATCTCGATCGGTTGAAGGTAAGTTCCTTCGAGCGCGTAACCCGACCTATCGAGCCGAAGGATTCGGGTCTGATC
ACGGAACGACTGGGGTTACT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSAIMFVGTASNSGKSFLAAVTCAYLRQRGVDVAPFKSQNMSLNSCVAKENGEIAVAQAFQAAMAGQEPSIHHNPVLLKP
KGELRSEVIVHGKPIGTMSYREYREIVFEDPWQAVLESAEILSEEHEVIVAEGAGSPAEINVLDTDIANLRVAEALGADV
ILVADISRGGAFAAVYGTIELLPERWRRLIKGFLFNKFLGDESLLEPGIKELERRLGVRYLGTVRHVGDFWMPWEDSEAL
DTHSPGRGSVRIAVIRLPRISNFTDFEPLAMEPDVRVEFVDPRDNLPEDADAVILPGTRTTISDLEELRKRGMDEEVVQA
ADEGTVVLGVCGGYQMLGRELVDESGGELDPGESVPGLGLLDAVTVFPSDAGKVTVRSEGVVNHPHLRGIRVEGFEIHEG
RTYTDEPHLVRLRSGYGNRGCFLDGAYRTDRPVLGTYLHGIFFNRRFRHEFLRWVSGGRWKPPERDVVREAVKRNLQVAL
EIVESTDLPELLGE

Sequences:

>Translated_494_residues
MSAIMFVGTASNSGKSFLAAVTCAYLRQRGVDVAPFKSQNMSLNSCVAKENGEIAVAQAFQAAMAGQEPSIHHNPVLLKP
KGELRSEVIVHGKPIGTMSYREYREIVFEDPWQAVLESAEILSEEHEVIVAEGAGSPAEINVLDTDIANLRVAEALGADV
ILVADISRGGAFAAVYGTIELLPERWRRLIKGFLFNKFLGDESLLEPGIKELERRLGVRYLGTVRHVGDFWMPWEDSEAL
DTHSPGRGSVRIAVIRLPRISNFTDFEPLAMEPDVRVEFVDPRDNLPEDADAVILPGTRTTISDLEELRKRGMDEEVVQA
ADEGTVVLGVCGGYQMLGRELVDESGGELDPGESVPGLGLLDAVTVFPSDAGKVTVRSEGVVNHPHLRGIRVEGFEIHEG
RTYTDEPHLVRLRSGYGNRGCFLDGAYRTDRPVLGTYLHGIFFNRRFRHEFLRWVSGGRWKPPERDVVREAVKRNLQVAL
EIVESTDLPELLGE
>Mature_493_residues
SAIMFVGTASNSGKSFLAAVTCAYLRQRGVDVAPFKSQNMSLNSCVAKENGEIAVAQAFQAAMAGQEPSIHHNPVLLKPK
GELRSEVIVHGKPIGTMSYREYREIVFEDPWQAVLESAEILSEEHEVIVAEGAGSPAEINVLDTDIANLRVAEALGADVI
LVADISRGGAFAAVYGTIELLPERWRRLIKGFLFNKFLGDESLLEPGIKELERRLGVRYLGTVRHVGDFWMPWEDSEALD
THSPGRGSVRIAVIRLPRISNFTDFEPLAMEPDVRVEFVDPRDNLPEDADAVILPGTRTTISDLEELRKRGMDEEVVQAA
DEGTVVLGVCGGYQMLGRELVDESGGELDPGESVPGLGLLDAVTVFPSDAGKVTVRSEGVVNHPHLRGIRVEGFEIHEGR
TYTDEPHLVRLRSGYGNRGCFLDGAYRTDRPVLGTYLHGIFFNRRFRHEFLRWVSGGRWKPPERDVVREAVKRNLQVALE
IVESTDLPELLGE

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_METKA (Q8TVH5)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614697.1
- ProteinModelPortal:   Q8TVH5
- GeneID:   1478009
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1414
- NMPDR:   fig|190192.1.peg.1410
- HOGENOM:   HBG302271
- OMA:   GVDVAPF
- BioCyc:   MKAN190192:MK1414-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 54562; Mature: 54430

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 331-331 ACT_SITE 439-439

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIMFVGTASNSGKSFLAAVTCAYLRQRGVDVAPFKSQNMSLNSCVAKENGEIAVAQAF
CCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHH
QAAMAGQEPSIHHNPVLLKPKGELRSEVIVHGKPIGTMSYREYREIVFEDPWQAVLESAE
HHHHCCCCCCCCCCCEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHH
ILSEEHEVIVAEGAGSPAEINVLDTDIANLRVAEALGADVILVADISRGGAFAAVYGTIE
HHCCCCCEEEEECCCCCCEEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHH
LLPERWRRLIKGFLFNKFLGDESLLEPGIKELERRLGVRYLGTVRHVGDFWMPWEDSEAL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCC
DTHSPGRGSVRIAVIRLPRISNFTDFEPLAMEPDVRVEFVDPRDNLPEDADAVILPGTRT
CCCCCCCCCEEEEEEEECCCCCCCCCCCCEECCCCEEEEECCCCCCCCCCCEEEECCCCC
TISDLEELRKRGMDEEVVQAADEGTVVLGVCGGYQMLGRELVDESGGELDPGESVPGLGL
HHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCH
LDAVTVFPSDAGKVTVRSEGVVNHPHLRGIRVEGFEIHEGRTYTDEPHLVRLRSGYGNRG
HHHHEECCCCCCEEEEECCCCCCCCCCCEEEEECEEEECCCCCCCCCCEEEEECCCCCCC
CFLDGAYRTDRPVLGTYLHGIFFNRRFRHEFLRWVSGGRWKPPERDVVREAVKRNLQVAL
CEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
EIVESTDLPELLGE
HHHHCCCCHHHHCC
>Mature Secondary Structure 
SAIMFVGTASNSGKSFLAAVTCAYLRQRGVDVAPFKSQNMSLNSCVAKENGEIAVAQAF
CEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHH
QAAMAGQEPSIHHNPVLLKPKGELRSEVIVHGKPIGTMSYREYREIVFEDPWQAVLESAE
HHHHCCCCCCCCCCCEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHH
ILSEEHEVIVAEGAGSPAEINVLDTDIANLRVAEALGADVILVADISRGGAFAAVYGTIE
HHCCCCCEEEEECCCCCCEEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCEEEEHHHHH
LLPERWRRLIKGFLFNKFLGDESLLEPGIKELERRLGVRYLGTVRHVGDFWMPWEDSEAL
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCC
DTHSPGRGSVRIAVIRLPRISNFTDFEPLAMEPDVRVEFVDPRDNLPEDADAVILPGTRT
CCCCCCCCCEEEEEEEECCCCCCCCCCCCEECCCCEEEEECCCCCCCCCCCEEEECCCCC
TISDLEELRKRGMDEEVVQAADEGTVVLGVCGGYQMLGRELVDESGGELDPGESVPGLGL
HHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCH
LDAVTVFPSDAGKVTVRSEGVVNHPHLRGIRVEGFEIHEGRTYTDEPHLVRLRSGYGNRG
HHHHEECCCCCCEEEEECCCCCCCCCCCEEEEECEEEECCCCCCCCCCEEEEECCCCCCC
CFLDGAYRTDRPVLGTYLHGIFFNRRFRHEFLRWVSGGRWKPPERDVVREAVKRNLQVAL
CEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
EIVESTDLPELLGE
HHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014