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The map label for this gene is ThiI_2 [H]

Identifier: 20094344

GI number: 20094344

Start: 862342

End: 863466

Strand: Reverse

Name: ThiI_2 [H]

Synonym: MK0908

Alternate gene names: 20094344

Gene position: 863466-862342 (Counterclockwise)

Preceding gene: 20094346

Following gene: 20094343

Centisome position: 50.94

GC content: 60.98

Gene sequence:

>1125_bases
GTGGAGTGCTTGGTAATGTACGGAGAGATAGCCGTTAAGTCCCCACCGGTCCGACGACGCATGGAGCGGCTGTTGGCCCG
GAATTTGGAAGAACAGTCCGGCGGGCGAGTGAAACGGTTAGAGGGTCGCCTGCTGGTCGCCGATCCGGAGGTTCCCGAAG
CGATCGGTAAAACCTTCGGTGTGGAACGGTGGACGCGCACGCTCCGCGTCGACTCTCACGACCCCGAGGACGTGTTCACG
GAAATGAAGGACGTACTGAACGACTTAAAGGCCCGATCGTTCGCCGTACGGGCCCGGAGAGCCACACACGACGCACCCTC
CTCCCGAGAGATGAACGTGGAACTCGGTGACCTGATCAGGCGATACACCGGTTGGACCGTGAACCTGGACGAGCCCGAGG
TTGAGATTCACGTGGAACTACGGCCCGAAGGGACCTTCGTGTACCTGGACTCGTGGATGAAGGATGGCCCGGGCGGTCTC
CCCTACGGCTCTCAGTCCCGCGTTGTATGCCTGGTATCCGGTGGTATCGACAGCCCTGTAGCCGCTTGGTACGCAGCTCG
TCGTGGATGTGAGGTGATCTGGCTGCATTTGGACCGGGGTAAGTACGGGTCGGAGGTGGACGCCGTAGAACGGCTCGCTG
GGAAGTTCTCCGAGTGGTTACCGTCCGACGTGGAGCTTTTGATCGAGGACTTCGAAGATTTCATGGAGAGTCTGGAGGGG
CTGGAGGGCGAATCGGCCCGATACCGATGCGTATTGTGCAAGCGGGAGATGATACGTAGGGCGTGTGACGTGTGCGAAAA
CGTCGGAGCCGTGGCCGTGGTCATGGGAGACGTCATCGGACAGGTGGCGAGCCAAATCCCGGATAACCTGGCGGTAATCG
ATCGCGTGGCACGGTTTCCGGTCTTCCGTCCGTTGTTGGGTTTCGATAAGAACGAGGTACAACGCCTCTCGGAACGGCTG
GGGCTTTTCGAGGTATCTAAGGAGCATCGCCCGTGTCCGCTGGCACCGCGCAACCCCGTGAAGTCAGCGGATCCGGGGAA
GGTGCTTAAGCTGGAGGAGGAACTGGGCGTGCTCCACCGCGGCCTGCGGGGTTCCGGCGTTGCCGGGTCGGAAGAGTATC
GATGA

Upstream 100 bases:

>100_bases
CGAGCAACGAATCCAGCAGTCCCAGCGTCGAACACCCCCTCCGTGCGTCACACAACCTCCAAAGCGTCCGTCCTGGCCGG
ACCCAGTCGGGGTAACCCTC

Downstream 100 bases:

>100_bases
GATCAACCGCCGAATCGAGCGTGGAGAGGTCTGTGTGGTGACCGCGGAGGAGATGGTGGAAATCGTCGAAGATATCGGGC
CCGAACGTGCCGCTAAAGAG

Product: ATP pyrophosphatase

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MECLVMYGEIAVKSPPVRRRMERLLARNLEEQSGGRVKRLEGRLLVADPEVPEAIGKTFGVERWTRTLRVDSHDPEDVFT
EMKDVLNDLKARSFAVRARRATHDAPSSREMNVELGDLIRRYTGWTVNLDEPEVEIHVELRPEGTFVYLDSWMKDGPGGL
PYGSQSRVVCLVSGGIDSPVAAWYAARRGCEVIWLHLDRGKYGSEVDAVERLAGKFSEWLPSDVELLIEDFEDFMESLEG
LEGESARYRCVLCKREMIRRACDVCENVGAVAVVMGDVIGQVASQIPDNLAVIDRVARFPVFRPLLGFDKNEVQRLSERL
GLFEVSKEHRPCPLAPRNPVKSADPGKVLKLEEELGVLHRGLRGSGVAGSEEYR

Sequences:

>Translated_374_residues
MECLVMYGEIAVKSPPVRRRMERLLARNLEEQSGGRVKRLEGRLLVADPEVPEAIGKTFGVERWTRTLRVDSHDPEDVFT
EMKDVLNDLKARSFAVRARRATHDAPSSREMNVELGDLIRRYTGWTVNLDEPEVEIHVELRPEGTFVYLDSWMKDGPGGL
PYGSQSRVVCLVSGGIDSPVAAWYAARRGCEVIWLHLDRGKYGSEVDAVERLAGKFSEWLPSDVELLIEDFEDFMESLEG
LEGESARYRCVLCKREMIRRACDVCENVGAVAVVMGDVIGQVASQIPDNLAVIDRVARFPVFRPLLGFDKNEVQRLSERL
GLFEVSKEHRPCPLAPRNPVKSADPGKVLKLEEELGVLHRGLRGSGVAGSEEYR
>Mature_374_residues
MECLVMYGEIAVKSPPVRRRMERLLARNLEEQSGGRVKRLEGRLLVADPEVPEAIGKTFGVERWTRTLRVDSHDPEDVFT
EMKDVLNDLKARSFAVRARRATHDAPSSREMNVELGDLIRRYTGWTVNLDEPEVEIHVELRPEGTFVYLDSWMKDGPGGL
PYGSQSRVVCLVSGGIDSPVAAWYAARRGCEVIWLHLDRGKYGSEVDAVERLAGKFSEWLPSDVELLIEDFEDFMESLEG
LEGESARYRCVLCKREMIRRACDVCENVGAVAVVMGDVIGQVASQIPDNLAVIDRVARFPVFRPLLGFDKNEVQRLSERL
GLFEVSKEHRPCPLAPRNPVKSADPGKVLKLEEELGVLHRGLRGSGVAGSEEYR

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=367, Percent_Identity=27.2479564032698, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 42019; Mature: 42019

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MECLVMYGEIAVKSPPVRRRMERLLARNLEEQSGGRVKRLEGRLLVADPEVPEAIGKTFG
CCEEEEECCEEECCCHHHHHHHHHHHHCCHHHCCCCEEEECCEEEEECCCCHHHHHHHHC
VERWTRTLRVDSHDPEDVFTEMKDVLNDLKARSFAVRARRATHDAPSSREMNVELGDLIR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RYTGWTVNLDEPEVEIHVELRPEGTFVYLDSWMKDGPGGLPYGSQSRVVCLVSGGIDSPV
HHCCCEEECCCCCEEEEEEECCCCCEEEEECHHCCCCCCCCCCCCCCEEEEEECCCCCHH
AAWYAARRGCEVIWLHLDRGKYGSEVDAVERLAGKFSEWLPSDVELLIEDFEDFMESLEG
HHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
LEGESARYRCVLCKREMIRRACDVCENVGAVAVVMGDVIGQVASQIPDNLAVIDRVARFP
CCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCH
VFRPLLGFDKNEVQRLSERLGLFEVSKEHRPCPLAPRNPVKSADPGKVLKLEEELGVLHR
HHHHHHCCCHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHC
GLRGSGVAGSEEYR
CCCCCCCCCCCCCC
>Mature Secondary Structure
MECLVMYGEIAVKSPPVRRRMERLLARNLEEQSGGRVKRLEGRLLVADPEVPEAIGKTFG
CCEEEEECCEEECCCHHHHHHHHHHHHCCHHHCCCCEEEECCEEEEECCCCHHHHHHHHC
VERWTRTLRVDSHDPEDVFTEMKDVLNDLKARSFAVRARRATHDAPSSREMNVELGDLIR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RYTGWTVNLDEPEVEIHVELRPEGTFVYLDSWMKDGPGGLPYGSQSRVVCLVSGGIDSPV
HHCCCEEECCCCCEEEEEEECCCCCEEEEECHHCCCCCCCCCCCCCCEEEEEECCCCCHH
AAWYAARRGCEVIWLHLDRGKYGSEVDAVERLAGKFSEWLPSDVELLIEDFEDFMESLEG
HHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
LEGESARYRCVLCKREMIRRACDVCENVGAVAVVMGDVIGQVASQIPDNLAVIDRVARFP
CCCCCCCEEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCH
VFRPLLGFDKNEVQRLSERLGLFEVSKEHRPCPLAPRNPVKSADPGKVLKLEEELGVLHR
HHHHHHCCCHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHC
GLRGSGVAGSEEYR
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]