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The map label for this gene is IspA [H]

Identifier: 20094211

GI number: 20094211

Start: 735536

End: 736510

Strand: Reverse

Name: IspA [H]

Synonym: MK0774

Alternate gene names: 20094211

Gene position: 736510-735536 (Counterclockwise)

Preceding gene: 20094212

Following gene: 20094210

Centisome position: 43.45

GC content: 58.46

Gene sequence:

>975_bases
ATGCGGAGTGAGATACTGAAAGAGCTGAACCGTGCACGCGAGCTCGTCGACGCCAAGATTGAGGAGGTACTACCCAGAGG
AGGCCCAGAGGACCTCTACGATGCGTGTTGGCACCTAATCGAAGCGGGAGGTAAGCGAATCCGACCCCTCCTCGCCATCA
AATCGTGTCTCATGTTAGGTGGATCGGAAGAGGACGTACTCCCTGAAGCGGTCGCGGTCGAGCTGATTCATACGTTCACT
CTAATTCACGACGACATCATGGACGAAGACGACGAACGACGCGGAGTCCCGGCGGTACACGTGAAGTGGGGAGTACCGGT
AGCCATACTGGCGGGAGACACCCTGTTCTCCAAAGCCTTCGAAGTGGCGGCGGAAGGCGGAGACGTAGAGGCAGTGAAAG
AACTCGCCCGCGCCTGCACGGAGATCTGTGAGGGCCAAGCCATGGACATCGGGTTCGAGAACAGGACTGAAGTCACCGAG
GATGAGTTCCTCGAAATGATCCGGAAGAAAACGGCAGCGCTCATACGGACATCGTGCGTCGTAGGCGGGCTTAAAGCCGG
GGCGAACCGCGAGCAACTGGAAGCGCTTCGGGAGTACGGCGAGAACTTGGGGATCGCGTTCCAGATACAGGACGACGTCC
TCGACCTTGTCGGAGACGAGTCCGAACTCGGTAAGCCCGTGGGCAGCGACATCGTCGAGGGGAAGAAGACGCTGATCGTG
ATCAAGGGCCTGGAGCTCGCCGATGAGGAGCAGCGGGAGCGCATACTTTCGGTCCTCGGGAACGAGGATGCTTCACGCGA
GGACGTTCGCGAGGTTATTCAGATACTCGAAGATCTCGGTGCGATAGATTACGCTAAAAAGCGGGCCCGAGAGTACGCCG
ATCGCGCTAAGGCTGCGCTGCGCGAGCTCCCCGAGAACGAGCATCGGGAGTTCCTAGAAAAGTTGGCGGATTTCGTTGTA
GAGAGGGAGTTCTGA

Upstream 100 bases:

>100_bases
CGCCGAACTGGCCACTCAGGAGGGTTACGAGGTGAACCGAGACGTATTCATAATGTTCGACGGCACGAAGCTAAGTCTGC
CGTTATAAGGGGACGATTCC

Downstream 100 bases:

>100_bases
AGTTGGCACTTCCCAGGATATACGTGGAGACCGACGAAGTTCCAGAGAGCGGCAAGATCTTGCTCACGGACCCCGAGTTC
GAGGTGATCGTACGCTCGCG

Product: geranylgeranyl pyrophosphate synthase

Products: NA

Alternate protein names: (2E,6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Farnesyl pyrophosphate synthase; FPP synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MRSEILKELNRARELVDAKIEEVLPRGGPEDLYDACWHLIEAGGKRIRPLLAIKSCLMLGGSEEDVLPEAVAVELIHTFT
LIHDDIMDEDDERRGVPAVHVKWGVPVAILAGDTLFSKAFEVAAEGGDVEAVKELARACTEICEGQAMDIGFENRTEVTE
DEFLEMIRKKTAALIRTSCVVGGLKAGANREQLEALREYGENLGIAFQIQDDVLDLVGDESELGKPVGSDIVEGKKTLIV
IKGLELADEEQRERILSVLGNEDASREDVREVIQILEDLGAIDYAKKRAREYADRAKAALRELPENEHREFLEKLADFVV
EREF

Sequences:

>Translated_324_residues
MRSEILKELNRARELVDAKIEEVLPRGGPEDLYDACWHLIEAGGKRIRPLLAIKSCLMLGGSEEDVLPEAVAVELIHTFT
LIHDDIMDEDDERRGVPAVHVKWGVPVAILAGDTLFSKAFEVAAEGGDVEAVKELARACTEICEGQAMDIGFENRTEVTE
DEFLEMIRKKTAALIRTSCVVGGLKAGANREQLEALREYGENLGIAFQIQDDVLDLVGDESELGKPVGSDIVEGKKTLIV
IKGLELADEEQRERILSVLGNEDASREDVREVIQILEDLGAIDYAKKRAREYADRAKAALRELPENEHREFLEKLADFVV
EREF
>Mature_324_residues
MRSEILKELNRARELVDAKIEEVLPRGGPEDLYDACWHLIEAGGKRIRPLLAIKSCLMLGGSEEDVLPEAVAVELIHTFT
LIHDDIMDEDDERRGVPAVHVKWGVPVAILAGDTLFSKAFEVAAEGGDVEAVKELARACTEICEGQAMDIGFENRTEVTE
DEFLEMIRKKTAALIRTSCVVGGLKAGANREQLEALREYGENLGIAFQIQDDVLDLVGDESELGKPVGSDIVEGKKTLIV
IKGLELADEEQRERILSVLGNEDASREDVREVIQILEDLGAIDYAKKRAREYADRAKAALRELPENEHREFLEKLADFVV
EREF

Specific function: Supplies Octaprenyl Diphosphate, The Precursor For The Side Chain Of The Isoprenoid Quinones Ubiquinone And Menaquinone. [C]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=295, Percent_Identity=28.135593220339, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI4758430, Length=260, Percent_Identity=26.1538461538462, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI83700220, Length=260, Percent_Identity=26.1538461538462, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1789578, Length=289, Percent_Identity=33.9100346020761, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1786623, Length=295, Percent_Identity=35.9322033898305, Blast_Score=144, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17505681, Length=256, Percent_Identity=30.859375, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17508563, Length=353, Percent_Identity=24.6458923512748, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319475, Length=295, Percent_Identity=28.8135593220339, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6325188, Length=274, Percent_Identity=24.8175182481752, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24651612, Length=297, Percent_Identity=26.2626262626263, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281365769, Length=285, Percent_Identity=27.3684210526316, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24660002, Length=285, Percent_Identity=27.3684210526316, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.1; =2.5.1.10 [H]

Molecular weight: Translated: 36063; Mature: 36063

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSEILKELNRARELVDAKIEEVLPRGGPEDLYDACWHLIEAGGKRIRPLLAIKSCLMLG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
GSEEDVLPEAVAVELIHTFTLIHDDIMDEDDERRGVPAVHVKWGVPVAILAGDTLFSKAF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEEECCCCEEEEECHHHHHHHH
EVAAEGGDVEAVKELARACTEICEGQAMDIGFENRTEVTEDEFLEMIRKKTAALIRTSCV
HHHHCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VGGLKAGANREQLEALREYGENLGIAFQIQDDVLDLVGDESELGKPVGSDIVEGKKTLIV
HHHHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCHHHHCCCCCHHHHCCCCEEEE
IKGLELADEEQRERILSVLGNEDASREDVREVIQILEDLGAIDYAKKRAREYADRAKAAL
EECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
RELPENEHREFLEKLADFVVEREF
HHCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSEILKELNRARELVDAKIEEVLPRGGPEDLYDACWHLIEAGGKRIRPLLAIKSCLMLG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHC
GSEEDVLPEAVAVELIHTFTLIHDDIMDEDDERRGVPAVHVKWGVPVAILAGDTLFSKAF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCEEEEECCCCEEEEECHHHHHHHH
EVAAEGGDVEAVKELARACTEICEGQAMDIGFENRTEVTEDEFLEMIRKKTAALIRTSCV
HHHHCCCCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VGGLKAGANREQLEALREYGENLGIAFQIQDDVLDLVGDESELGKPVGSDIVEGKKTLIV
HHHHHCCCCHHHHHHHHHHCCCCCEEEEEHHHHHHHHCCHHHHCCCCCHHHHCCCCEEEE
IKGLELADEEQRERILSVLGNEDASREDVREVIQILEDLGAIDYAKKRAREYADRAKAAL
EECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
RELPENEHREFLEKLADFVVEREF
HHCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7979381 [H]