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The map label for this gene is top6A [H]

Identifier: 20093950

GI number: 20093950

Start: 476375

End: 477415

Strand: Reverse

Name: top6A [H]

Synonym: MK0512

Alternate gene names: 20093950

Gene position: 477415-476375 (Counterclockwise)

Preceding gene: 20093951

Following gene: 20093947

Centisome position: 28.17

GC content: 58.89

Gene sequence:

>1041_bases
ATGTCCGAAGCCCTACAACGCCTACGTGAGTTCGCGAAACAGGTCCTGAAGTGCGTGGAGAAGGACGAACCAGTCCGGAT
ACCGAAACGTACGACGTCCAACATCCGTTACGACCCCGAGCGTGAGATATTCACGCTCGGTCCCGATAAGCTCGAGCGCC
GTCCACGCACCCTGAAAGGCTCCAAGAAGTTGGCTCAGATGCTAAGTGTCGCCGCCTTCGCCAAGGAGCTCGTGGAGAAG
GGCCGCTCCGCCACCCTCAGGGAGATTTACTACACCAGCGAAGGTTGGGAAGTCGACTTCAAGGATCAGCGCGAGAGCGA
CGCGATCGTGGAGGACTTGGAAGCGACGCTAGGTCTCAAGCGTGAGGAGTTCGGTGTATGGCCCGAAGAGGACGGCGCCG
CCGTGTACGGCGACCTGCTAGTCCGGGAAGACGGCGTCGAGATCCACGCCGAGCGAGCCGGGATCAGCGGCTACAACATC
CCGCCCAACGTCGATGCCGTTGAGATACTGGATTGTGGCGCCGAACGCGTGATCGCCGTTGAGACTATGGGTATGTACCG
TAGGCTCGTTCAGGAGCGCGCCCACGAGAAGCTGAACGCGCTGATCGTGGGACTGAAGGGTCAAGCCGCTCGGGCGACCA
GACGACTGCTCCGACGTCTGAACGAGGAGCTGGGATTGCCCGTGTACGTGTTCACGGACGGCGATCCCTACGGCTGGCAC
ATTTACATGGTGATAAGGAGTGGAAGTGCCAAAGCCGCTCATCTCAACGAGGAGCTCGCGTGTCCTGACGCTAAATTCAT
CGGCGTCACGGCCACCGATATCGTCGAGTACGACCTTCCCACGGAACCCCTGAAGAAGACCGATTACAAGAGAATAGAGG
AGCTGCGCCGAGATCCGCGGTACTACGACCCGTTCTGGAAGAAAGAACTGGATTTATTGGAAAAACTCGGGAAGAAGGCA
GAGCAGCAGGCGTTCGCGAAGTATTCCCTGGACTACGTCGTCAAGGAGTACCTGCCTGCCAAGCTCGACGAGCTGGAATG
A

Upstream 100 bases:

>100_bases
TCGTAAGATGAAGCGCGAGAAGCTGCGCCTGTGGAAGGACTCGGAGCCCCCCGACATCCTCTAACACCTCGCCTCTCAGA
GCACCCGAGGGAACTACAGG

Downstream 100 bases:

>100_bases
ACGCCAGCGCTACCATCAGCGGCGATACTGGTATCCCGTGCTTCTTGGCAGGCACCTTCTGCAGCCACGCACCCAGTGCG
GCTAGGCACCAGCCCGTACA

Product: DNA topoisomerase VI subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSEALQRLREFAKQVLKCVEKDEPVRIPKRTTSNIRYDPEREIFTLGPDKLERRPRTLKGSKKLAQMLSVAAFAKELVEK
GRSATLREIYYTSEGWEVDFKDQRESDAIVEDLEATLGLKREEFGVWPEEDGAAVYGDLLVREDGVEIHAERAGISGYNI
PPNVDAVEILDCGAERVIAVETMGMYRRLVQERAHEKLNALIVGLKGQAARATRRLLRRLNEELGLPVYVFTDGDPYGWH
IYMVIRSGSAKAAHLNEELACPDAKFIGVTATDIVEYDLPTEPLKKTDYKRIEELRRDPRYYDPFWKKELDLLEKLGKKA
EQQAFAKYSLDYVVKEYLPAKLDELE

Sequences:

>Translated_346_residues
MSEALQRLREFAKQVLKCVEKDEPVRIPKRTTSNIRYDPEREIFTLGPDKLERRPRTLKGSKKLAQMLSVAAFAKELVEK
GRSATLREIYYTSEGWEVDFKDQRESDAIVEDLEATLGLKREEFGVWPEEDGAAVYGDLLVREDGVEIHAERAGISGYNI
PPNVDAVEILDCGAERVIAVETMGMYRRLVQERAHEKLNALIVGLKGQAARATRRLLRRLNEELGLPVYVFTDGDPYGWH
IYMVIRSGSAKAAHLNEELACPDAKFIGVTATDIVEYDLPTEPLKKTDYKRIEELRRDPRYYDPFWKKELDLLEKLGKKA
EQQAFAKYSLDYVVKEYLPAKLDELE
>Mature_345_residues
SEALQRLREFAKQVLKCVEKDEPVRIPKRTTSNIRYDPEREIFTLGPDKLERRPRTLKGSKKLAQMLSVAAFAKELVEKG
RSATLREIYYTSEGWEVDFKDQRESDAIVEDLEATLGLKREEFGVWPEEDGAAVYGDLLVREDGVEIHAERAGISGYNIP
PNVDAVEILDCGAERVIAVETMGMYRRLVQERAHEKLNALIVGLKGQAARATRRLLRRLNEELGLPVYVFTDGDPYGWHI
YMVIRSGSAKAAHLNEELACPDAKFIGVTATDIVEYDLPTEPLKKTDYKRIEELRRDPRYYDPFWKKELDLLEKLGKKAE
QQAFAKYSLDYVVKEYLPAKLDELE

Specific function: Relaxes both positive and negative superturns and exhibits a strong decatenase activity [H]

COG id: COG1697

COG function: function code L; DNA topoisomerase VI, subunit A

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TOP6A family [H]

Homologues:

Organism=Homo sapiens, GI38201680, Length=290, Percent_Identity=27.9310344827586, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI6912680, Length=290, Percent_Identity=27.9310344827586, Blast_Score=132, Evalue=5e-31,
Organism=Drosophila melanogaster, GI45552753, Length=188, Percent_Identity=30.3191489361702, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002815
- InterPro:   IPR013049
- InterPro:   IPR004085
- InterPro:   IPR022904
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04406 TP6A_N [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 39672; Mature: 39541

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS01034 GLYCOSYL_HYDROL_F16

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEALQRLREFAKQVLKCVEKDEPVRIPKRTTSNIRYDPEREIFTLGPDKLERRPRTLKG
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHCCCCCCCC
SKKLAQMLSVAAFAKELVEKGRSATLREIYYTSEGWEVDFKDQRESDAIVEDLEATLGLK
HHHHHHHHHHHHHHHHHHHCCCCCEEHHEEECCCCCEECCCCCCCHHHHHHHHHHHHCCC
REEFGVWPEEDGAAVYGDLLVREDGVEIHAERAGISGYNIPPNVDAVEILDCGAERVIAV
HHHCCCCCCCCCCEEEEHHEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCCCEEEH
ETMGMYRRLVQERAHEKLNALIVGLKGQAARATRRLLRRLNEELGLPVYVFTDGDPYGWH
HHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEE
IYMVIRSGSAKAAHLNEELACPDAKFIGVTATDIVEYDLPTEPLKKTDYKRIEELRRDPR
EEEEEECCCCCHHHCCCHHCCCCCCEEECCHHHHEECCCCCCCHHHCHHHHHHHHHHCCC
YYDPFWKKELDLLEKLGKKAEQQAFAKYSLDYVVKEYLPAKLDELE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
SEALQRLREFAKQVLKCVEKDEPVRIPKRTTSNIRYDPEREIFTLGPDKLERRPRTLKG
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHCCCCCCCC
SKKLAQMLSVAAFAKELVEKGRSATLREIYYTSEGWEVDFKDQRESDAIVEDLEATLGLK
HHHHHHHHHHHHHHHHHHHCCCCCEEHHEEECCCCCEECCCCCCCHHHHHHHHHHHHCCC
REEFGVWPEEDGAAVYGDLLVREDGVEIHAERAGISGYNIPPNVDAVEILDCGAERVIAV
HHHCCCCCCCCCCEEEEHHEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHCCCCCEEEH
ETMGMYRRLVQERAHEKLNALIVGLKGQAARATRRLLRRLNEELGLPVYVFTDGDPYGWH
HHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCEE
IYMVIRSGSAKAAHLNEELACPDAKFIGVTATDIVEYDLPTEPLKKTDYKRIEELRRDPR
EEEEEECCCCCHHHCCCHHCCCCCCEEECCHHHHEECCCCCCCHHHCHHHHHHHHHHCCC
YYDPFWKKELDLLEKLGKKAEQQAFAKYSLDYVVKEYLPAKLDELE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]