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The map label for this gene is aubA

Identifier: 20093937

GI number: 20093937

Start: 460425

End: 461879

Strand: Reverse

Name: aubA

Synonym: MK0499

Alternate gene names: 20093937

Gene position: 461879-460425 (Counterclockwise)

Preceding gene: 20093944

Following gene: 20093936

Centisome position: 27.25

GC content: 59.24

Gene sequence:

>1455_bases
GTGCCCTGTAAGGTCAGGATACGTGGTATATACTCGACGGCGCTCACCAAGATCTGCCTGGATCACGGGTTCATAGTCAC
CCAGCCCTCCGATGACATTAGGCGGCGGTTCCCCGATGCGGAATTCGACTCCGGTTCGCCCGATGTGGACGTTCGGGACA
CGAGGAATCGACACGGCATCGAGATCCAGGGTCCGGCGGATGATGTTCGAGAGCTCGTGGACATCCTCCAATCGGAGGTC
TGGGCTACAGTTACGGCCGACAAGATCGGAGAAGGGAGCGTGTTCAAGGGCGTGGTCCGTGAAATCGATGATCGGGCCGG
GGTTGCCGTCGTAGATCTCGGGAACGGACTTCAGGGTTTCCTCAGCGAGGACGAGTCGGAGGTCGTGGAAGAGGGAGAGG
AGCTCGTAGTACAGGTTGCGAAGTCCGTGTCGGACGGCCCACTCAAGCTGACGACGGAGGTCACGGTGGCCGGCGAGTAC
GCCGTCCTGGTACCCGTCGAGGGGATCAGAGTCAGCCGCAAAATCCGGGACGAACGCGAGCGTGAACGTCTGAAGCGTCT
CGGCGAGGCGTTGGTCCCGGAAGGATGGGGATTGATCTGGAGGACCGCCGCCGAGGGTAAGTCCGGGGAAGAGCTGGCGG
AGGAGATCGACGATCTAATCGAGGAACGTAAGCAACTGTTCAAGCGTGCCGAGGAGATGTCCGAACCCGGTCCCATCCGA
GATGTGCGTGAGATGGAGTTGGAGATCCATTCGCTCGCCAAGAGCCGGCTCGACTCCGTGCGCTCCGAAGTCCTACCGAC
TATGGTGGGACACCACTACTTCAAGTGTCGCAGCCTCGCCGGTAGCGTGGCCGTGGATACCGTGGAACCGTTCCTGGATG
ACCTTGACGAGGAGGTCGTTGCGGAGCGCCTGATCCGATGCCTGACCAGGTCGGAAGGACCGTCCGAAGGCGATAGAATC
GACATCGTCCACGTGAAACCGGGACAGGGAGTCAAGAAGCTCGGTGGGAACCCTAAGGTAGTAGAGTACGATCCCGTGGA
GGGGATCCTCAAGGTCCGTCGTGAGATGCGTGGACCCGGCTTCTACGATGGAATCGACAAACCGATCGAGAAAGGTGATT
ACGCCATCTCAATACTCCCCGACGGTTCCATGGTCACGGTTCATCAGTATTTCAACAAAGACGGTGAGCTGAAGGGTCGT
TACTACAACATAGGAACGCCGCTGGAGGTATTCAAGAACTGCGTGCGGTACGTGGATTTGGAGGTCGACGTCGTCGAACC
CGAGGAAGGGGAGCGCGAAATAATCGACGAGGAGGATCTGGAGCGCGCCGTCGACTCCGGTCTTATCCCGGAAGAACTCG
CGGAGCTCGCACTGGAGACCGCTAAGAGGGTCGAAAAACGTGGGATGAAGGAGGTCAAGCCCTACCCCGTGTGGAAAGGA
TTCGAGCACGGTTGA

Upstream 100 bases:

>100_bases
GATCCCTCCGGACGCTCCTCGATACGGTTACGGATCACTTCGTAGACCTCGAGGAGAACCTCGGGGTCTCCCATCCGCTT
CCCCCGGGGGGGTGCTCTGG

Downstream 100 bases:

>100_bases
ACCTAGGGGAAACGTTGTTGGAATGGGAGCGTGTGTTGGCCCGGTGTGACGGTTGCGGGGCTTGCGAATCCAGATGTCCG
AACGACGTACCCAGAAGATT

Product: ribonuclease of the G/E family

Products: NA

Alternate protein names: AU-binding protein

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MPCKVRIRGIYSTALTKICLDHGFIVTQPSDDIRRRFPDAEFDSGSPDVDVRDTRNRHGIEIQGPADDVRELVDILQSEV
WATVTADKIGEGSVFKGVVREIDDRAGVAVVDLGNGLQGFLSEDESEVVEEGEELVVQVAKSVSDGPLKLTTEVTVAGEY
AVLVPVEGIRVSRKIRDERERERLKRLGEALVPEGWGLIWRTAAEGKSGEELAEEIDDLIEERKQLFKRAEEMSEPGPIR
DVREMELEIHSLAKSRLDSVRSEVLPTMVGHHYFKCRSLAGSVAVDTVEPFLDDLDEEVVAERLIRCLTRSEGPSEGDRI
DIVHVKPGQGVKKLGGNPKVVEYDPVEGILKVRREMRGPGFYDGIDKPIEKGDYAISILPDGSMVTVHQYFNKDGELKGR
YYNIGTPLEVFKNCVRYVDLEVDVVEPEEGEREIIDEEDLERAVDSGLIPEELAELALETAKRVEKRGMKEVKPYPVWKG
FEHG

Sequences:

>Translated_484_residues
MPCKVRIRGIYSTALTKICLDHGFIVTQPSDDIRRRFPDAEFDSGSPDVDVRDTRNRHGIEIQGPADDVRELVDILQSEV
WATVTADKIGEGSVFKGVVREIDDRAGVAVVDLGNGLQGFLSEDESEVVEEGEELVVQVAKSVSDGPLKLTTEVTVAGEY
AVLVPVEGIRVSRKIRDERERERLKRLGEALVPEGWGLIWRTAAEGKSGEELAEEIDDLIEERKQLFKRAEEMSEPGPIR
DVREMELEIHSLAKSRLDSVRSEVLPTMVGHHYFKCRSLAGSVAVDTVEPFLDDLDEEVVAERLIRCLTRSEGPSEGDRI
DIVHVKPGQGVKKLGGNPKVVEYDPVEGILKVRREMRGPGFYDGIDKPIEKGDYAISILPDGSMVTVHQYFNKDGELKGR
YYNIGTPLEVFKNCVRYVDLEVDVVEPEEGEREIIDEEDLERAVDSGLIPEELAELALETAKRVEKRGMKEVKPYPVWKG
FEHG
>Mature_483_residues
PCKVRIRGIYSTALTKICLDHGFIVTQPSDDIRRRFPDAEFDSGSPDVDVRDTRNRHGIEIQGPADDVRELVDILQSEVW
ATVTADKIGEGSVFKGVVREIDDRAGVAVVDLGNGLQGFLSEDESEVVEEGEELVVQVAKSVSDGPLKLTTEVTVAGEYA
VLVPVEGIRVSRKIRDERERERLKRLGEALVPEGWGLIWRTAAEGKSGEELAEEIDDLIEERKQLFKRAEEMSEPGPIRD
VREMELEIHSLAKSRLDSVRSEVLPTMVGHHYFKCRSLAGSVAVDTVEPFLDDLDEEVVAERLIRCLTRSEGPSEGDRID
IVHVKPGQGVKKLGGNPKVVEYDPVEGILKVRREMRGPGFYDGIDKPIEKGDYAISILPDGSMVTVHQYFNKDGELKGRY
YNIGTPLEVFKNCVRYVDLEVDVVEPEEGEREIIDEEDLERAVDSGLIPEELAELALETAKRVEKRGMKEVKPYPVWKGF
EHG

Specific function: Binds to RNA in loop regions with AU-rich sequences

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Escherichia coli, GI87082235, Length=147, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AUBA_METKA (Q8TY07)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613784.1
- HSSP:   P21513
- ProteinModelPortal:   Q8TY07
- SMR:   Q8TY07
- GeneID:   1476600
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0499
- NMPDR:   fig|190192.1.peg.497
- HOGENOM:   HBG705374
- OMA:   RGIYSTA
- ProtClustDB:   CLSK2747645
- BioCyc:   MKAN190192:MK0499-MONOMER
- HAMAP:   MF_01910
- InterPro:   IPR007295
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR016730
- InterPro:   IPR022967
- PIRSF:   PIRSF018644
- SMART:   SM00316

Pfam domain/function: PF04167 DUF402; PF10150 RNase_E_G; SSF50249 Nucleic_acid_OB

EC number: 3.1.4.- [C]

Molecular weight: Translated: 54157; Mature: 54026

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCKVRIRGIYSTALTKICLDHGFIVTQPSDDIRRRFPDAEFDSGSPDVDVRDTRNRHGI
CCCEEEEHHHHHHHHHHHHHHCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCE
EIQGPADDVRELVDILQSEVWATVTADKIGEGSVFKGVVREIDDRAGVAVVDLGNGLQGF
EEECCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHH
LSEDESEVVEEGEELVVQVAKSVSDGPLKLTTEVTVAGEYAVLVPVEGIRVSRKIRDERE
HCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEEEECCCHHHHHHHHHHHH
RERLKRLGEALVPEGWGLIWRTAAEGKSGEELAEEIDDLIEERKQLFKRAEEMSEPGPIR
HHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DVREMELEIHSLAKSRLDSVRSEVLPTMVGHHYFKCRSLAGSVAVDTVEPFLDDLDEEVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHH
AERLIRCLTRSEGPSEGDRIDIVHVKPGQGVKKLGGNPKVVEYDPVEGILKVRREMRGPG
HHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHCCCCCCEEEECCHHHHHHHHHHHCCCC
FYDGIDKPIEKGDYAISILPDGSMVTVHQYFNKDGELKGRYYNIGTPLEVFKNCVRYVDL
CCCCCCCHHHCCCEEEEEECCCCEEEEHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCC
EVDVVEPEEGEREIIDEEDLERAVDSGLIPEELAELALETAKRVEKRGMKEVKPYPVWKG
EEEEECCCCCCHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCC
FEHG
CCCC
>Mature Secondary Structure 
PCKVRIRGIYSTALTKICLDHGFIVTQPSDDIRRRFPDAEFDSGSPDVDVRDTRNRHGI
CCEEEEHHHHHHHHHHHHHHCCEEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCE
EIQGPADDVRELVDILQSEVWATVTADKIGEGSVFKGVVREIDDRAGVAVVDLGNGLQGF
EEECCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHH
LSEDESEVVEEGEELVVQVAKSVSDGPLKLTTEVTVAGEYAVLVPVEGIRVSRKIRDERE
HCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEEEECCCHHHHHHHHHHHH
RERLKRLGEALVPEGWGLIWRTAAEGKSGEELAEEIDDLIEERKQLFKRAEEMSEPGPIR
HHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DVREMELEIHSLAKSRLDSVRSEVLPTMVGHHYFKCRSLAGSVAVDTVEPFLDDLDEEVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHH
AERLIRCLTRSEGPSEGDRIDIVHVKPGQGVKKLGGNPKVVEYDPVEGILKVRREMRGPG
HHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHHCCCCCCEEEECCHHHHHHHHHHHCCCC
FYDGIDKPIEKGDYAISILPDGSMVTVHQYFNKDGELKGRYYNIGTPLEVFKNCVRYVDL
CCCCCCCHHHCCCEEEEEECCCCEEEEHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHCC
EVDVVEPEEGEREIIDEEDLERAVDSGLIPEELAELALETAKRVEKRGMKEVKPYPVWKG
EEEEECCCCCCHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCC
FEHG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014