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The map label for this gene is PurL_2

Identifier: 20093935

GI number: 20093935

Start: 457283

End: 459457

Strand: Reverse

Name: PurL_2

Synonym: MK0497

Alternate gene names: 20093935

Gene position: 459457-457283 (Counterclockwise)

Preceding gene: 20093936

Following gene: 20093934

Centisome position: 27.11

GC content: 62.76

Gene sequence:

>2175_bases
TTGACGTTGGACGAACGCGATCTGGAGCTCATCCGGGAGGAACTAGGCAGGGACCCCAACGCCACGGAGCGAGCCATGTT
CGAGAACATGTGGTCGGAACACTGCGCCTACCGTAGCACTCGCCACTTACTTCGACAGCTCCCCAGCGAGGCGGATCACG
TCATCGTGGGTCCCGGCGATGACGCCGCCGTGGTCGCCATCGACGACGAGTGGGCCGTAGTAGTCGGTATAGAAAGCCAC
AACCACCCCTCCTACGTAGATCCGTACAACGGGGCGGCGACCGGTGTCGGCGGGATAGTCCGAGACGTTCTGTCCATGGG
AGCGTTCCCGATAGCTCTCCTGGATCCGCTTCGCTTCGGCCCGCTGGACGGAGAACGCGTCCCCTACCTCGTCGACGGTG
TCGTCCGCGGGATCTCGGATTACGGTAACCGCATCGGAGTCCCGACGGTAGGTGGAGAGCTGGAGTTCGATCCTTCTTAC
GAGCGCAACCCCCTCGTCAACGTGATGTGTGTGGGTATAGTTCGCAGGAGTGAGATCGTACGAGGTCGTGCGGACCGCCC
CGGGGACGTACTGGTACTAGTCGGCGCTCGAACGGGCCGTGACGGGATCGGCGGTGCGGCCTTCGCTTCCGAAGAGCTGG
GCGAGGAATCGGAGGAAGAAGACAGGCCGGCGGTCCAAATCGGCGACCCGTTCACGGAGCGTCAACTGATCATCGCCATC
CGGGAAGCCGTCGAGCGTGGCCTAGTCAAAGGGTGCAAGGACCTAGGTGCCGCCGGTCTGACGTGCGCCGCGACCGAGAT
GGCCGCCGACGGGGGGACCGGCGTCGAGATCGACGTCTTTAAGGTCCCGCTCCGGGAGGAGGGGATGGAACCGTGGGAGA
TCATGCTCTCGGAATCCCAGGAGAGAATGCTGCTCGTCGTGGCCCCTGAGGATGTGGACGAGGTGATAGAGATCTGCCGT
AAGTACGGTCTCGAGGCGTCGGTCGTCGGCAGGGTTACTGACGACGGCTACCTCACGGTTAAGGACGGTGACGACGTCAT
CGCTCGGGTTCCCGCCGAGTTCCTGGCCGACGGTGCTCCCGAGGTCGAGTGGGAGGAGGAGCCTTATTCGTACCCCGAGA
ACGTGGACGTGCCGGAGCCGGACCCCGAGGATCTAGTGCGCTCAGTCCTGAGCTCCCCCAACGTTTCGCCGGCGCTTCGC
GAGTGGGTTTACCGGCAGTATGACCATGAGGTGCAAGGACGCACCGTCGTGAAACCGGGTCACGATGCTGCCGTGATGTG
GTTGCAGCACGAAGGCCTGGAAGACGTGGCCCTGGCCCTGACGACTGATTCGAATCCGCGGCACGTGCTCATCGATCCGA
AAACGGGTACGGAAGGGTGCGTCGCCGAAGCGTTACGTAACCTCGCCACCGTCGGAGCGGAGCCGCTCTGTCTGGTTGAC
TGCTTGAACTTCGGAAGCCCGGAAAACCCGCGGGTCTATTACCAGCTTCGCCGGTCGATCGAGGGGCTCGGAAAAGCAGC
CCGCGAGTTCGAGGTTCCTGTAGTCGGCGGAAACGTGAGCCTGTACAACGAACACGAGGTCGACGGCCCGGTGAATCCCA
CGCCCGTGATAGGCGCCGTGGGAGTGATCCGGGGATTGGACTATCTAGAAGACTTCCCTCGAGAGCCCGAAGAAGGCGAG
GCGGTGATAGTCTTAGGGGAGACCCGGGAGGAGCTCGGCGGGTCCCTGTACCTCATCGAGTACCATGGTATTAAGGGAGG
TAAGGTACCACGCGTGAGGTACAGGGAGGAGCGAGCCCTGCACGACCTGCTCCGCCGTATCGCGAGGAAGAATATGGTTT
CTTCAGTCACTGACGTGTCCACCGGGGGACTTCTAGCTGCCGTAGCCGAGCTACTGGGACCTGTCGGAGCCTCCCTGAGC
CTGTCCGAAGTCCCGAATTCCGTGTCACGGTGGGACTTCCTGCTGTTGTCCGAGAGCCACGGTCGGGCTATCGTGACTAC
GGACCGACCCGACGATGTGTTGGGGGCTGCTGAAGAGGCCGGTGTACCGGCTCAGGTGGTAGGGGAGGTCACCGGAGACG
GCGTCCTGCGGATCTCGGTGGGACCCGTGGACGTATCACTGGATCGAGAGGAGCTGGAAGAGCTGTGGAGATCCCCGTTG
CACTACTTGGAGTAG

Upstream 100 bases:

>100_bases
CGCTGTGGGATCACGTCGTCGATTTATCCCGATGACGAACCCGCTGTCTGCCGACCACTCCTAGGGTGACGAGGTTACGC
ACTCCCTGAGGGGTCTCGGC

Downstream 100 bases:

>100_bases
GGGGTGCTACGACTGCCGGAGTTCCTGGTGTTCATCGATCATCCGTCCAAGAAGGAGGTCCCCGACTCCGAAGTGGAAGT
GGCGAGAAGGCGCGTCGAAC

Product: phosphoribosylformylglycinamidine synthase domain-containing protein

Products: NA

Alternate protein names: Phosphoribosylformylglycinamidine synthase II; FGAM synthase II

Number of amino acids: Translated: 724; Mature: 723

Protein sequence:

>724_residues
MTLDERDLELIREELGRDPNATERAMFENMWSEHCAYRSTRHLLRQLPSEADHVIVGPGDDAAVVAIDDEWAVVVGIESH
NHPSYVDPYNGAATGVGGIVRDVLSMGAFPIALLDPLRFGPLDGERVPYLVDGVVRGISDYGNRIGVPTVGGELEFDPSY
ERNPLVNVMCVGIVRRSEIVRGRADRPGDVLVLVGARTGRDGIGGAAFASEELGEESEEEDRPAVQIGDPFTERQLIIAI
REAVERGLVKGCKDLGAAGLTCAATEMAADGGTGVEIDVFKVPLREEGMEPWEIMLSESQERMLLVVAPEDVDEVIEICR
KYGLEASVVGRVTDDGYLTVKDGDDVIARVPAEFLADGAPEVEWEEEPYSYPENVDVPEPDPEDLVRSVLSSPNVSPALR
EWVYRQYDHEVQGRTVVKPGHDAAVMWLQHEGLEDVALALTTDSNPRHVLIDPKTGTEGCVAEALRNLATVGAEPLCLVD
CLNFGSPENPRVYYQLRRSIEGLGKAAREFEVPVVGGNVSLYNEHEVDGPVNPTPVIGAVGVIRGLDYLEDFPREPEEGE
AVIVLGETREELGGSLYLIEYHGIKGGKVPRVRYREERALHDLLRRIARKNMVSSVTDVSTGGLLAAVAELLGPVGASLS
LSEVPNSVSRWDFLLLSESHGRAIVTTDRPDDVLGAAEEAGVPAQVVGEVTGDGVLRISVGPVDVSLDREELEELWRSPL
HYLE

Sequences:

>Translated_724_residues
MTLDERDLELIREELGRDPNATERAMFENMWSEHCAYRSTRHLLRQLPSEADHVIVGPGDDAAVVAIDDEWAVVVGIESH
NHPSYVDPYNGAATGVGGIVRDVLSMGAFPIALLDPLRFGPLDGERVPYLVDGVVRGISDYGNRIGVPTVGGELEFDPSY
ERNPLVNVMCVGIVRRSEIVRGRADRPGDVLVLVGARTGRDGIGGAAFASEELGEESEEEDRPAVQIGDPFTERQLIIAI
REAVERGLVKGCKDLGAAGLTCAATEMAADGGTGVEIDVFKVPLREEGMEPWEIMLSESQERMLLVVAPEDVDEVIEICR
KYGLEASVVGRVTDDGYLTVKDGDDVIARVPAEFLADGAPEVEWEEEPYSYPENVDVPEPDPEDLVRSVLSSPNVSPALR
EWVYRQYDHEVQGRTVVKPGHDAAVMWLQHEGLEDVALALTTDSNPRHVLIDPKTGTEGCVAEALRNLATVGAEPLCLVD
CLNFGSPENPRVYYQLRRSIEGLGKAAREFEVPVVGGNVSLYNEHEVDGPVNPTPVIGAVGVIRGLDYLEDFPREPEEGE
AVIVLGETREELGGSLYLIEYHGIKGGKVPRVRYREERALHDLLRRIARKNMVSSVTDVSTGGLLAAVAELLGPVGASLS
LSEVPNSVSRWDFLLLSESHGRAIVTTDRPDDVLGAAEEAGVPAQVVGEVTGDGVLRISVGPVDVSLDREELEELWRSPL
HYLE
>Mature_723_residues
TLDERDLELIREELGRDPNATERAMFENMWSEHCAYRSTRHLLRQLPSEADHVIVGPGDDAAVVAIDDEWAVVVGIESHN
HPSYVDPYNGAATGVGGIVRDVLSMGAFPIALLDPLRFGPLDGERVPYLVDGVVRGISDYGNRIGVPTVGGELEFDPSYE
RNPLVNVMCVGIVRRSEIVRGRADRPGDVLVLVGARTGRDGIGGAAFASEELGEESEEEDRPAVQIGDPFTERQLIIAIR
EAVERGLVKGCKDLGAAGLTCAATEMAADGGTGVEIDVFKVPLREEGMEPWEIMLSESQERMLLVVAPEDVDEVIEICRK
YGLEASVVGRVTDDGYLTVKDGDDVIARVPAEFLADGAPEVEWEEEPYSYPENVDVPEPDPEDLVRSVLSSPNVSPALRE
WVYRQYDHEVQGRTVVKPGHDAAVMWLQHEGLEDVALALTTDSNPRHVLIDPKTGTEGCVAEALRNLATVGAEPLCLVDC
LNFGSPENPRVYYQLRRSIEGLGKAAREFEVPVVGGNVSLYNEHEVDGPVNPTPVIGAVGVIRGLDYLEDFPREPEEGEA
VIVLGETREELGGSLYLIEYHGIKGGKVPRVRYREERALHDLLRRIARKNMVSSVTDVSTGGLLAAVAELLGPVGASLSL
SEVPNSVSRWDFLLLSESHGRAIVTTDRPDDVLGAAEEAGVPAQVVGEVTGDGVLRISVGPVDVSLDREELEELWRSPLH
YLE

Specific function: Unknown

COG id: COG0046

COG function: function code F; Phosphoribosylformylglycinamidine (FGAM) synthase, synthetase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGAMS family

Homologues:

Organism=Homo sapiens, GI31657129, Length=459, Percent_Identity=27.2331154684096, Blast_Score=97, Evalue=5e-20,
Organism=Escherichia coli, GI48994899, Length=419, Percent_Identity=26.2529832935561, Blast_Score=115, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI17553022, Length=806, Percent_Identity=24.9379652605459, Blast_Score=115, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6321498, Length=735, Percent_Identity=24.8979591836735, Blast_Score=137, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24582111, Length=833, Percent_Identity=23.8895558223289, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24582109, Length=833, Percent_Identity=23.8895558223289, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137292, Length=833, Percent_Identity=23.8895558223289, Blast_Score=98, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURL_METKA (Q8TY09)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613782.1
- ProteinModelPortal:   Q8TY09
- GeneID:   1476598
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0497
- NMPDR:   fig|190192.1.peg.495
- HOGENOM:   HBG311214
- OMA:   YGNSFGV
- BioCyc:   MKAN190192:MK0497-MONOMER
- BRENDA:   6.3.5.3
- GO:   GO:0005737
- HAMAP:   MF_00420
- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR010074
- InterPro:   IPR016188
- TIGRFAMs:   TIGR01736

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C; SSF56042 AIR_synth_C; SSF55326 PurM_N-like

EC number: =6.3.5.3

Molecular weight: Translated: 78778; Mature: 78647

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDERDLELIREELGRDPNATERAMFENMWSEHCAYRSTRHLLRQLPSEADHVIVGPGD
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
DAAVVAIDDEWAVVVGIESHNHPSYVDPYNGAATGVGGIVRDVLSMGAFPIALLDPLRFG
CEEEEEECCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCC
PLDGERVPYLVDGVVRGISDYGNRIGVPTVGGELEFDPSYERNPLVNVMCVGIVRRSEIV
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHH
RGRADRPGDVLVLVGARTGRDGIGGAAFASEELGEESEEEDRPAVQIGDPFTERQLIIAI
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCHHCCCEEEECCCCCHHHHHHHH
REAVERGLVKGCKDLGAAGLTCAATEMAADGGTGVEIDVFKVPLREEGMEPWEIMLSESQ
HHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCEEEEEECCHHHCCCCHHHHHHCCCC
ERMLLVVAPEDVDEVIEICRKYGLEASVVGRVTDDGYLTVKDGDDVIARVPAEFLADGAP
CCEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCHHHHCCHHHHCCCCC
EVEWEEEPYSYPENVDVPEPDPEDLVRSVLSSPNVSPALREWVYRQYDHEVQGRTVVKPG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEECCC
HDAAVMWLQHEGLEDVALALTTDSNPRHVLIDPKTGTEGCVAEALRNLATVGAEPLCLVD
CCEEEEEEECCCCCEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHH
CLNFGSPENPRVYYQLRRSIEGLGKAAREFEVPVVGGNVSLYNEHEVDGPVNPTPVIGAV
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEECCCCCCCCCCCCCHHHHH
GVIRGLDYLEDFPREPEEGEAVIVLGETREELGGSLYLIEYHGIKGGKVPRVRYREERAL
HHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHCCEEEEEEEECCCCCCCCCHHHHHHHHH
HDLLRRIARKNMVSSVTDVSTGGLLAAVAELLGPVGASLSLSEVPNSVSRWDFLLLSESH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHCCCCHHHCEEEEEECCC
GRAIVTTDRPDDVLGAAEEAGVPAQVVGEVTGDGVLRISVGPVDVSLDREELEELWRSPL
CCEEEECCCCCHHHCCHHHCCCCHHHHHHCCCCCEEEEEECCEEEECCHHHHHHHHHHHH
HYLE
HHCC
>Mature Secondary Structure 
TLDERDLELIREELGRDPNATERAMFENMWSEHCAYRSTRHLLRQLPSEADHVIVGPGD
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
DAAVVAIDDEWAVVVGIESHNHPSYVDPYNGAATGVGGIVRDVLSMGAFPIALLDPLRFG
CEEEEEECCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCC
PLDGERVPYLVDGVVRGISDYGNRIGVPTVGGELEFDPSYERNPLVNVMCVGIVRRSEIV
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHH
RGRADRPGDVLVLVGARTGRDGIGGAAFASEELGEESEEEDRPAVQIGDPFTERQLIIAI
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHCCCCCHHCCCEEEECCCCCHHHHHHHH
REAVERGLVKGCKDLGAAGLTCAATEMAADGGTGVEIDVFKVPLREEGMEPWEIMLSESQ
HHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCCCCEEEEEECCHHHCCCCHHHHHHCCCC
ERMLLVVAPEDVDEVIEICRKYGLEASVVGRVTDDGYLTVKDGDDVIARVPAEFLADGAP
CCEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCEEEEECCCCHHHHCCHHHHCCCCC
EVEWEEEPYSYPENVDVPEPDPEDLVRSVLSSPNVSPALREWVYRQYDHEVQGRTVVKPG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEECCC
HDAAVMWLQHEGLEDVALALTTDSNPRHVLIDPKTGTEGCVAEALRNLATVGAEPLCLVD
CCEEEEEEECCCCCEEEEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHH
CLNFGSPENPRVYYQLRRSIEGLGKAAREFEVPVVGGNVSLYNEHEVDGPVNPTPVIGAV
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEECCCCCCCCCCCCCHHHHH
GVIRGLDYLEDFPREPEEGEAVIVLGETREELGGSLYLIEYHGIKGGKVPRVRYREERAL
HHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHCCEEEEEEEECCCCCCCCCHHHHHHHHH
HDLLRRIARKNMVSSVTDVSTGGLLAAVAELLGPVGASLSLSEVPNSVSRWDFLLLSESH
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHCCCCHHHCEEEEEECCC
GRAIVTTDRPDDVLGAAEEAGVPAQVVGEVTGDGVLRISVGPVDVSLDREELEELWRSPL
CCEEEECCCCCHHHCCHHHCCCCHHHHHHCCCCCEEEEEECCEEEECCHHHHHHHHHHHH
HYLE
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014