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The map label for this gene is CarA

Identifier: 20093929

GI number: 20093929

Start: 452156

End: 453241

Strand: Reverse

Name: CarA

Synonym: MK0491

Alternate gene names: 20093929

Gene position: 453241-452156 (Counterclockwise)

Preceding gene: 20093931

Following gene: 20093928

Centisome position: 26.74

GC content: 59.02

Gene sequence:

>1086_bases
TTGAGGGCGGCACTCGCGCTTGAGGATGGGACGATAGTACATGGCGAACTTTTCGGGAGTCCGCGCGAGGCCGAAGGGGA
AGTCGTGTTTAACACTTCTCACACTGGCTTCCAGGAAGCTCTGACAGATCCGTCCTACCGCGGACAGATCCTAATCATGA
CGTTCCCGATGCAAGGCAACTATGGGATCTTACCGGAAGTCGGCGAGTCCGACAGGGTGCAAGTGGAGGGCTTCGTCGTG
CGGTACCTCCACGATGGTCCCATCCATCCGCGCGCCGAAATCACATTGGACGAATTCCTCCAGGACCACGGCGTCCCGGG
GATCGCGGGCGTCGACACGCGGATGCTGACGAGGAAAATCCGGACGGAGGGTGCCATGAGAGGGGTTCTGGTACCCTACG
AACCGGACGACCAACCTTCCGATGAAGAACTACTGGAGCGAGTGCGGGAAGTTCCCCATATCTCGGAGATGGATCTGGTG
CCGCAGGTCTCGGTCCGTGAGGAATACCGGTTCTCCGACGGAAAACCGGAGATCGTGGTCATAGATTGCGGTGTGAAGCG
GAGCATACTGCGTGAGCTGGCCAAACGCGGCGCCGGCGTTACCGTAGTCCCGTACGACACTTCGGCCCAGGAGATAATGG
ACATCGATCCCGACGGTGTCGTAGTATCCAACGGACCGGGCGACCCCAAACGCGTCAGGGAGACCGTGGAAACGGTCCGT
GAACTGATAGGTCAGGTTCCATTGATGGGTATATGTCTTGGCAACCAAATCCTCGGACTCGCCGAGGGGGGCGATACTTT
CAAGCTGAAGTTCGGGCACCGTGGCGCGAACCAGCCCGTCAAGGATCTTGATAAGGATAGAGTCTACATCACTTCCCAGA
ACCATGGATTCGCACTGGATCCGGACAGTCTCAGAGACACGCCCTTGAGAGTCCGCTGGGTTAACGTCAACGACGGTACG
GTCGAAGGCGTCGTTCACACGGACGCCCCGGCGTTTTCCGTGCAGTTCCACCCGGAGGCAGGGCCCGGTCCTTGGGATAC
CAAGTGGGTGTTCGACGAGTTCCTGGCCATGTGTCGCGAACACTGA

Upstream 100 bases:

>100_bases
GACACCCGTCGCAGCAGCGGTCTCAGCTCCCTTATCAACGTGGGCCCCCCGGTGTAGCTTTTATCACCAAGATCCTAGGA
AACTCCGAGGGTTCGTCCGG

Downstream 100 bases:

>100_bases
CCTCCGGGGAACCCCCGTTGGAACGCATCGTCGTACTGATACCAGCTAAGCACGAATCAGTGGAAGCTGTGGAGAAACCT
CTCCGTTCCGTCTTATCGCA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MRAALALEDGTIVHGELFGSPREAEGEVVFNTSHTGFQEALTDPSYRGQILIMTFPMQGNYGILPEVGESDRVQVEGFVV
RYLHDGPIHPRAEITLDEFLQDHGVPGIAGVDTRMLTRKIRTEGAMRGVLVPYEPDDQPSDEELLERVREVPHISEMDLV
PQVSVREEYRFSDGKPEIVVIDCGVKRSILRELAKRGAGVTVVPYDTSAQEIMDIDPDGVVVSNGPGDPKRVRETVETVR
ELIGQVPLMGICLGNQILGLAEGGDTFKLKFGHRGANQPVKDLDKDRVYITSQNHGFALDPDSLRDTPLRVRWVNVNDGT
VEGVVHTDAPAFSVQFHPEAGPGPWDTKWVFDEFLAMCREH

Sequences:

>Translated_361_residues
MRAALALEDGTIVHGELFGSPREAEGEVVFNTSHTGFQEALTDPSYRGQILIMTFPMQGNYGILPEVGESDRVQVEGFVV
RYLHDGPIHPRAEITLDEFLQDHGVPGIAGVDTRMLTRKIRTEGAMRGVLVPYEPDDQPSDEELLERVREVPHISEMDLV
PQVSVREEYRFSDGKPEIVVIDCGVKRSILRELAKRGAGVTVVPYDTSAQEIMDIDPDGVVVSNGPGDPKRVRETVETVR
ELIGQVPLMGICLGNQILGLAEGGDTFKLKFGHRGANQPVKDLDKDRVYITSQNHGFALDPDSLRDTPLRVRWVNVNDGT
VEGVVHTDAPAFSVQFHPEAGPGPWDTKWVFDEFLAMCREH
>Mature_361_residues
MRAALALEDGTIVHGELFGSPREAEGEVVFNTSHTGFQEALTDPSYRGQILIMTFPMQGNYGILPEVGESDRVQVEGFVV
RYLHDGPIHPRAEITLDEFLQDHGVPGIAGVDTRMLTRKIRTEGAMRGVLVPYEPDDQPSDEELLERVREVPHISEMDLV
PQVSVREEYRFSDGKPEIVVIDCGVKRSILRELAKRGAGVTVVPYDTSAQEIMDIDPDGVVVSNGPGDPKRVRETVETVR
ELIGQVPLMGICLGNQILGLAEGGDTFKLKFGHRGANQPVKDLDKDRVYITSQNHGFALDPDSLRDTPLRVRWVNVNDGT
VEGVVHTDAPAFSVQFHPEAGPGPWDTKWVFDEFLAMCREH

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=375, Percent_Identity=44, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI21361331, Length=375, Percent_Identity=39.7333333333333, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI169790915, Length=375, Percent_Identity=39.7333333333333, Blast_Score=260, Evalue=2e-69,
Organism=Escherichia coli, GI1786215, Length=377, Percent_Identity=42.7055702917772, Blast_Score=296, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI193204318, Length=374, Percent_Identity=41.1764705882353, Blast_Score=266, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6324878, Length=375, Percent_Identity=40, Blast_Score=273, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6322331, Length=392, Percent_Identity=38.265306122449, Blast_Score=260, Evalue=2e-70,
Organism=Drosophila melanogaster, GI45555749, Length=372, Percent_Identity=43.010752688172, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24642586, Length=372, Percent_Identity=43.010752688172, Blast_Score=262, Evalue=3e-70,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_METKA (Q8TY15)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613776.1
- ProteinModelPortal:   Q8TY15
- SMR:   Q8TY15
- GeneID:   1477794
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0491
- NMPDR:   fig|190192.1.peg.489
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BioCyc:   MKAN190192:MK0491-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_A
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 39999; Mature: 39999

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 252-252 ACT_SITE 337-337 ACT_SITE 339-339

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAALALEDGTIVHGELFGSPREAEGEVVFNTSHTGFQEALTDPSYRGQILIMTFPMQGN
CCCEEEECCCCEEEEEECCCCCCCCCCEEEECCCCCHHHHHCCCCCCCEEEEEEECCCCC
YGILPEVGESDRVQVEGFVVRYLHDGPIHPRAEITLDEFLQDHGVPGIAGVDTRMLTRKI
CCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHH
RTEGAMRGVLVPYEPDDQPSDEELLERVREVPHISEMDLVPQVSVREEYRFSDGKPEIVV
HHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCEEE
IDCGVKRSILRELAKRGAGVTVVPYDTSAQEIMDIDPDGVVVSNGPGDPKRVRETVETVR
EECCCHHHHHHHHHHCCCCEEEEECCCCHHHHEECCCCCEEEECCCCCHHHHHHHHHHHH
ELIGQVPLMGICLGNQILGLAEGGDTFKLKFGHRGANQPVKDLDKDRVYITSQNHGFALD
HHHHCCCEEEEEECCCEEEEECCCCEEEEEECCCCCCCCHHHCCCCEEEEEECCCCEEEC
PDSLRDTPLRVRWVNVNDGTVEGVVHTDAPAFSVQFHPEAGPGPWDTKWVFDEFLAMCRE
CCCCCCCCEEEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHC
H
C
>Mature Secondary Structure
MRAALALEDGTIVHGELFGSPREAEGEVVFNTSHTGFQEALTDPSYRGQILIMTFPMQGN
CCCEEEECCCCEEEEEECCCCCCCCCCEEEECCCCCHHHHHCCCCCCCEEEEEEECCCCC
YGILPEVGESDRVQVEGFVVRYLHDGPIHPRAEITLDEFLQDHGVPGIAGVDTRMLTRKI
CCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHH
RTEGAMRGVLVPYEPDDQPSDEELLERVREVPHISEMDLVPQVSVREEYRFSDGKPEIVV
HHHHCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCCCCEEE
IDCGVKRSILRELAKRGAGVTVVPYDTSAQEIMDIDPDGVVVSNGPGDPKRVRETVETVR
EECCCHHHHHHHHHHCCCCEEEEECCCCHHHHEECCCCCEEEECCCCCHHHHHHHHHHHH
ELIGQVPLMGICLGNQILGLAEGGDTFKLKFGHRGANQPVKDLDKDRVYITSQNHGFALD
HHHHCCCEEEEEECCCEEEEECCCCEEEEEECCCCCCCCHHHCCCCEEEEEECCCCEEEC
PDSLRDTPLRVRWVNVNDGTVEGVVHTDAPAFSVQFHPEAGPGPWDTKWVFDEFLAMCRE
CCCCCCCCEEEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHC
H
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014