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The map label for this gene is EhaN [C]

Identifier: 20093902

GI number: 20093902

Start: 430599

End: 431087

Strand: Reverse

Name: EhaN [C]

Synonym: MK0464

Alternate gene names: 20093902

Gene position: 431087-430599 (Counterclockwise)

Preceding gene: 20093903

Following gene: 20093901

Centisome position: 25.43

GC content: 53.17

Gene sequence:

>489_bases
ATGAGCCTGAAGTCAGCGCTTAAAAAATTGAAAGGGTTTGTGAGATCTAGGTCTATTCATGTGACAGTAGTGAACACTGG
TGGGTGTAATGGATGCGATATTGAAATTTTAGCTTGCTACACTTATCGATACGATCTAGAACAGTACGGTATCTATTACC
ACAATAATCCGAGGAAATCTGACGTTCTAATAGTTACTGGACCAGTTACGTATCAATGGCGAGATAAGCTAGTCAAGCTG
TACCACAAGGTCCCCGAGCCCAAGGCCGTCGTCGCTGTGGGCACCTGCGCGTGCTCTGGCGGCATCTTCAACCAGCGAGG
AGGCCGCGTATGTGGGCCGGTACGCGAGGTGATCCCCGTAGACGCTGAGGTTCCAGGATGTCCACCACGGCCCGAGGAGA
TAGTCTCCGCGGTCGTCGACGTGCTTCCTGCTTTGTTCCGCAGTTGGGAGTTCCGTAGGGGTGGGGCCCGTGAAGCGCAG
GAGGCGTGA

Upstream 100 bases:

>100_bases
AGGATGAACTACTGCGAATGTTTGAAGGACGAAAATCCGTGCCGATAGACCGTAAGGAATTAACAAAGTTCTTCACTTCT
CTCCGTGAGAGGGGGATTAT

Downstream 100 bases:

>100_bases
GATCCAATCCAACGTCCAAGTCGTGGACGTCGAGACGGGTGAGATCGAGCCGATATCACACCGTGTCCCCGTGGGTCCTA
ATCATCCGATCCTCAAAGAG

Product: Ni,Fe-hydrogenase III small subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 162; Mature: 161

Protein sequence:

>162_residues
MSLKSALKKLKGFVRSRSIHVTVVNTGGCNGCDIEILACYTYRYDLEQYGIYYHNNPRKSDVLIVTGPVTYQWRDKLVKL
YHKVPEPKAVVAVGTCACSGGIFNQRGGRVCGPVREVIPVDAEVPGCPPRPEEIVSAVVDVLPALFRSWEFRRGGAREAQ
EA

Sequences:

>Translated_162_residues
MSLKSALKKLKGFVRSRSIHVTVVNTGGCNGCDIEILACYTYRYDLEQYGIYYHNNPRKSDVLIVTGPVTYQWRDKLVKL
YHKVPEPKAVVAVGTCACSGGIFNQRGGRVCGPVREVIPVDAEVPGCPPRPEEIVSAVVDVLPALFRSWEFRRGGAREAQ
EA
>Mature_161_residues
SLKSALKKLKGFVRSRSIHVTVVNTGGCNGCDIEILACYTYRYDLEQYGIYYHNNPRKSDVLIVTGPVTYQWRDKLVKLY
HKVPEPKAVVAVGTCACSGGIFNQRGGRVCGPVREVIPVDAEVPGCPPRPEEIVSAVVDVLPALFRSWEFRRGGAREAQE
A

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=116, Percent_Identity=36.2068965517241, Blast_Score=88, Evalue=3e-18,
Organism=Escherichia coli, GI1789074, Length=158, Percent_Identity=37.3417721518987, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1788834, Length=138, Percent_Identity=36.231884057971, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1788624, Length=113, Percent_Identity=33.6283185840708, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17509685, Length=125, Percent_Identity=35.2, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI18859983, Length=116, Percent_Identity=37.9310344827586, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24642371, Length=116, Percent_Identity=37.9310344827586, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24651058, Length=116, Percent_Identity=36.2068965517241, Blast_Score=87, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 17926; Mature: 17795

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKSALKKLKGFVRSRSIHVTVVNTGGCNGCDIEILACYTYRYDLEQYGIYYHNNPRKS
CCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEEEEEEEHHHCCEEEECCCCCC
DVLIVTGPVTYQWRDKLVKLYHKVPEPKAVVAVGTCACSGGIFNQRGGRVCGPVREVIPV
CEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHEEC
DAEVPGCPPRPEEIVSAVVDVLPALFRSWEFRRGGAREAQEA
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SLKSALKKLKGFVRSRSIHVTVVNTGGCNGCDIEILACYTYRYDLEQYGIYYHNNPRKS
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEEEEEEEHHHCCEEEECCCCCC
DVLIVTGPVTYQWRDKLVKLYHKVPEPKAVVAVGTCACSGGIFNQRGGRVCGPVREVIPV
CEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCHHHHEEC
DAEVPGCPPRPEEIVSAVVDVLPALFRSWEFRRGGAREAQEA
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]