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The map label for this gene is EhaO [C]

Identifier: 20093901

GI number: 20093901

Start: 429389

End: 430618

Strand: Reverse

Name: EhaO [C]

Synonym: MK0463

Alternate gene names: 20093901

Gene position: 430618-429389 (Counterclockwise)

Preceding gene: 20093902

Following gene: 20093900

Centisome position: 25.41

GC content: 58.21

Gene sequence:

>1230_bases
GTGAAGCGCAGGAGGCGTGAGATCCAATCCAACGTCCAAGTCGTGGACGTCGAGACGGGTGAGATCGAGCCGATATCACA
CCGTGTCCCCGTGGGTCCTAATCATCCGATCCTCAAAGAGCCCCTCCGGATCAAGCTGGCGGTCCGAGGAGAAGAGGTTG
TGGACTGCAAGGTGGAGATGGGATACTGCCACCGTGGTATCGAGAAGATCATGGAGGGAATGCCCTGGCAGAAGGCCGCC
TTCCTCGCTGAACGAGTCTGCGGAATCTGCTCCCACGCGCACAACATGTGCTTCATCGGAGGTGTCGAGAAACTCGCCGA
AGGAGATCCCGCTCCGCGTGGATTGTTCCTCCGCGTCCTCGTGCAAGAGCTAGATAGGATTCAATCCCACCTCATCGCCA
ACGCCGCCTACTTCTACTCGATAGAGCATGAGACTATGTTCGTGTGGAACATGAACACTCGTGAGCGCGTCCTGGACTGC
ATCGAAGAGATAACCGGTAACCGGATCCTGACGGGTTGGAACGTCGTCGGTGGTGTTCGAATGGACGTCACCGAGGACCA
ACTGAACAACGTCCTCGAAACCCTGGACGCAATCCGTGACGACGTGATAACGTACCGACGAATCGCCAAGAACGATCCCT
TCATCAAGCTCCGATCGCGTGGTGTCGGGGTGATCACTAAGGACCATATCAGGAAGTACCGAGTCGTTGGGCCGCAGGCT
AGGGCTTCCGGAGTCCCCGAGTCTGACATGCGTCTCCAAGAGCCCGTTTATCCGGAGCTGGGCTTCAAGCCCGTTTACAG
GAAGGAGGGGGACAACCTCGCCAGGATCTTGGTCCGATACGACGAGTGTTTGCAGTCCATAGAGCTCATCGAGCGGATCG
TCGATGAGTTGCCCGAAGGGAGGCATCGACGGGAGCTGGAGGTGAACGCGGGACACGTCGATAACCGCGTCGAGGCCCCA
CGAGGGGAACTCGTGTACGATATGGAACTAGCTCCTGGAGGAGTCGTGAAGCGCGTTACGATCCGCACACCTTCCGTCCC
GAATATCCGAGTCCTGGAGGCGATAGCGCCTGGATCTCCTAGCATCGCAGACGCCGTAGCCACATATGCGAGCTTGGACC
CGTGCGTCGCGTGCAATGAGAGGTTCGTGGTGATCGATGAACGGGAGGGTAAGGTTCTCTTAGAAGGGAAGGCGCGGGAG
GTGATCCGCGCTTGCTCCAGGACTTCCTGA

Upstream 100 bases:

>100_bases
GTTCCAGGATGTCCACCACGGCCCGAGGAGATAGTCTCCGCGGTCGTCGACGTGCTTCCTGCTTTGTTCCGCAGTTGGGA
GTTCCGTAGGGGTGGGGCCC

Downstream 100 bases:

>100_bases
ACCACATATTGAGCCGGGAAGGATCCAAGCGTCTGCTCGACGCTCACACCTCCCGAGAGATCATGCAAAGACCTCCCAGG
TTCCGGGACTTCCCGGACGT

Product: Ni,Fe-hydrogenase III large subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MKRRRREIQSNVQVVDVETGEIEPISHRVPVGPNHPILKEPLRIKLAVRGEEVVDCKVEMGYCHRGIEKIMEGMPWQKAA
FLAERVCGICSHAHNMCFIGGVEKLAEGDPAPRGLFLRVLVQELDRIQSHLIANAAYFYSIEHETMFVWNMNTRERVLDC
IEEITGNRILTGWNVVGGVRMDVTEDQLNNVLETLDAIRDDVITYRRIAKNDPFIKLRSRGVGVITKDHIRKYRVVGPQA
RASGVPESDMRLQEPVYPELGFKPVYRKEGDNLARILVRYDECLQSIELIERIVDELPEGRHRRELEVNAGHVDNRVEAP
RGELVYDMELAPGGVVKRVTIRTPSVPNIRVLEAIAPGSPSIADAVATYASLDPCVACNERFVVIDEREGKVLLEGKARE
VIRACSRTS

Sequences:

>Translated_409_residues
MKRRRREIQSNVQVVDVETGEIEPISHRVPVGPNHPILKEPLRIKLAVRGEEVVDCKVEMGYCHRGIEKIMEGMPWQKAA
FLAERVCGICSHAHNMCFIGGVEKLAEGDPAPRGLFLRVLVQELDRIQSHLIANAAYFYSIEHETMFVWNMNTRERVLDC
IEEITGNRILTGWNVVGGVRMDVTEDQLNNVLETLDAIRDDVITYRRIAKNDPFIKLRSRGVGVITKDHIRKYRVVGPQA
RASGVPESDMRLQEPVYPELGFKPVYRKEGDNLARILVRYDECLQSIELIERIVDELPEGRHRRELEVNAGHVDNRVEAP
RGELVYDMELAPGGVVKRVTIRTPSVPNIRVLEAIAPGSPSIADAVATYASLDPCVACNERFVVIDEREGKVLLEGKARE
VIRACSRTS
>Mature_409_residues
MKRRRREIQSNVQVVDVETGEIEPISHRVPVGPNHPILKEPLRIKLAVRGEEVVDCKVEMGYCHRGIEKIMEGMPWQKAA
FLAERVCGICSHAHNMCFIGGVEKLAEGDPAPRGLFLRVLVQELDRIQSHLIANAAYFYSIEHETMFVWNMNTRERVLDC
IEEITGNRILTGWNVVGGVRMDVTEDQLNNVLETLDAIRDDVITYRRIAKNDPFIKLRSRGVGVITKDHIRKYRVVGPQA
RASGVPESDMRLQEPVYPELGFKPVYRKEGDNLARILVRYDECLQSIELIERIVDELPEGRHRRELEVNAGHVDNRVEAP
RGELVYDMELAPGGVVKRVTIRTPSVPNIRVLEAIAPGSPSIADAVATYASLDPCVACNERFVVIDEREGKVLLEGKARE
VIRACSRTS

Specific function: Unknown

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=376, Percent_Identity=24.7340425531915, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI260898743, Length=370, Percent_Identity=24.8648648648649, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1788832, Length=368, Percent_Identity=29.3478260869565, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1789076, Length=370, Percent_Identity=30.2702702702703, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI145693162, Length=383, Percent_Identity=26.8929503916449, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17555284, Length=381, Percent_Identity=23.8845144356955, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17568379, Length=381, Percent_Identity=24.4094488188976, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24638644, Length=383, Percent_Identity=26.3707571801567, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI221459469, Length=380, Percent_Identity=25.5263157894737, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR014029
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 46195; Mature: 46195

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00507 NI_HGENASE_L_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRRREIQSNVQVVDVETGEIEPISHRVPVGPNHPILKEPLRIKLAVRGEEVVDCKVEM
CCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEEH
GYCHRGIEKIMEGMPWQKAAFLAERVCGICSHAHNMCFIGGVEKLAEGDPAPRGLFLRVL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHCCCCCCHHHHHHHH
VQELDRIQSHLIANAAYFYSIEHETMFVWNMNTRERVLDCIEEITGNRILTGWNVVGGVR
HHHHHHHHHHHHHCEEEEEEEECCEEEEEECCHHHHHHHHHHHHCCCEEEECCEEECCEE
MDVTEDQLNNVLETLDAIRDDVITYRRIAKNDPFIKLRSRGVGVITKDHIRKYRVVGPQA
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEHHHHHHHHCCCCHH
RASGVPESDMRLQEPVYPELGFKPVYRKEGDNLARILVRYDECLQSIELIERIVDELPEG
HHCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RHRRELEVNAGHVDNRVEAPRGELVYDMELAPGGVVKRVTIRTPSVPNIRVLEAIAPGSP
CCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCEEEEEEECCCCC
SIADAVATYASLDPCVACNERFVVIDEREGKVLLEGKAREVIRACSRTS
HHHHHHHHHHCCCCCEECCCEEEEEECCCCEEEECCHHHHHHHHHCCCC
>Mature Secondary Structure
MKRRRREIQSNVQVVDVETGEIEPISHRVPVGPNHPILKEPLRIKLAVRGEEVVDCKVEM
CCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEEEH
GYCHRGIEKIMEGMPWQKAAFLAERVCGICSHAHNMCFIGGVEKLAEGDPAPRGLFLRVL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHCCCCCCHHHHHHHH
VQELDRIQSHLIANAAYFYSIEHETMFVWNMNTRERVLDCIEEITGNRILTGWNVVGGVR
HHHHHHHHHHHHHCEEEEEEEECCEEEEEECCHHHHHHHHHHHHCCCEEEECCEEECCEE
MDVTEDQLNNVLETLDAIRDDVITYRRIAKNDPFIKLRSRGVGVITKDHIRKYRVVGPQA
ECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEHHHHHHHHCCCCHH
RASGVPESDMRLQEPVYPELGFKPVYRKEGDNLARILVRYDECLQSIELIERIVDELPEG
HHCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
RHRRELEVNAGHVDNRVEAPRGELVYDMELAPGGVVKRVTIRTPSVPNIRVLEAIAPGSP
CCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCEEEEEEECCCCC
SIADAVATYASLDPCVACNERFVVIDEREGKVLLEGKAREVIRACSRTS
HHHHHHHHHHCCCCCEECCCEEEEEECCCCEEEECCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S Cluster. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]