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The map label for this gene is SUI2

Identifier: 20093857

GI number: 20093857

Start: 394890

End: 395693

Strand: Direct

Name: SUI2

Synonym: MK0419

Alternate gene names: 20093857

Gene position: 394890-395693 (Clockwise)

Preceding gene: 20093856

Following gene: 20093858

Centisome position: 23.3

GC content: 59.08

Gene sequence:

>804_bases
ATGCCCCGGAAGTTACGCGATCTTCCCGAAGAAGGCGAGATAGTGATGGCCACGGTAGAGCGTGTCGAGGACCACGGAGC
CTTCGTCACGCTGGACGAGTACCCGGGAGTGGACGGGTACATCCATATCTCGGAGGTAGCGAGCGGTTGGGTCAAGAACA
TCAGGGACTACGTCAAGGAGGGTCAGAAGGTAGTCGCCAAGGTTATCCGCGTGAACCCGAAGCGCAAGTACGCCAACCTA
TCCCTCAGGAAGGTCACCGACCACCAGCGCAAGGAGAAACTGAAGGAGTGGAAGCGTGAGCAGAGGGCCGAAAAGCTCCT
AGAAATGGCCGCAGAAGAGCTCGGCAAGGATCTGGACGAGGCTTACGAGGAGGCTGGGTACAAACTGATCGAAGAATACG
GATCCCTTTACGATGCACTGGAAAGGGCGGCCGCCGAGGAAGGGCCGGAACCACTCCTCAAGGCCGGAGTACCGGAAGAA
TGGGCCGAAAAGCTCGCGGAGCTGGCGATTGAGAACATCGAGCCGGGTCGCGTGAAGATCGAGGCGTACGTCGATCTCAC
ATGCCCGGCTCCGAACGGTGTGGAGATAATCCGTGAGGCACTCGAGAAGATCGAAGAGTTCCAGCAAGGCGACGTGAAAA
TGGAAGTACAGTACGTAGGAGCTCCCCGGTACCGGATCACCGTGGACGCTCCGGACTACCGAACGGCAGAGAAAATGGTC
AGGAAGGCAGCTCAGGCCGCGATCGACCACGTGGAAGAGCACGGAGGCGAGGGCGAGTTCCACCGGGAGATCGAGGAGGG
GTAA

Upstream 100 bases:

>100_bases
CGAGCACCGAGGTAAAGTGCCACATCTGCGGCCGCACCCTAGCCAAGCCGACCGGCGGTAAGGCCAAGATCCTCACTAAG
ATCAAGGAGGTGCTCGAGTA

Downstream 100 bases:

>100_bases
AGCGTGCCTAAACGACTACGCAGGTGTAAGGAGTGCGGGGAATACACGCTTCAAAGGGACAAATGTCCGCATTGCGGTGG
AGATCTGGAGGTGCCGCACC

Product: translation initiation factor IF-2 subunit alpha

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: aIF2-alpha; eIF-2-alpha

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MPRKLRDLPEEGEIVMATVERVEDHGAFVTLDEYPGVDGYIHISEVASGWVKNIRDYVKEGQKVVAKVIRVNPKRKYANL
SLRKVTDHQRKEKLKEWKREQRAEKLLEMAAEELGKDLDEAYEEAGYKLIEEYGSLYDALERAAAEEGPEPLLKAGVPEE
WAEKLAELAIENIEPGRVKIEAYVDLTCPAPNGVEIIREALEKIEEFQQGDVKMEVQYVGAPRYRITVDAPDYRTAEKMV
RKAAQAAIDHVEEHGGEGEFHREIEEG

Sequences:

>Translated_267_residues
MPRKLRDLPEEGEIVMATVERVEDHGAFVTLDEYPGVDGYIHISEVASGWVKNIRDYVKEGQKVVAKVIRVNPKRKYANL
SLRKVTDHQRKEKLKEWKREQRAEKLLEMAAEELGKDLDEAYEEAGYKLIEEYGSLYDALERAAAEEGPEPLLKAGVPEE
WAEKLAELAIENIEPGRVKIEAYVDLTCPAPNGVEIIREALEKIEEFQQGDVKMEVQYVGAPRYRITVDAPDYRTAEKMV
RKAAQAAIDHVEEHGGEGEFHREIEEG
>Mature_266_residues
PRKLRDLPEEGEIVMATVERVEDHGAFVTLDEYPGVDGYIHISEVASGWVKNIRDYVKEGQKVVAKVIRVNPKRKYANLS
LRKVTDHQRKEKLKEWKREQRAEKLLEMAAEELGKDLDEAYEEAGYKLIEEYGSLYDALERAAAEEGPEPLLKAGVPEEW
AEKLAELAIENIEPGRVKIEAYVDLTCPAPNGVEIIREALEKIEEFQQGDVKMEVQYVGAPRYRITVDAPDYRTAEKMVR
KAAQAAIDHVEEHGGEGEFHREIEEG

Specific function: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA

COG id: COG1093

COG function: function code J; Translation initiation factor 2, alpha subunit (eIF-2alpha)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI4758256, Length=258, Percent_Identity=25.5813953488372, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17509871, Length=232, Percent_Identity=25.8620689655172, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322466, Length=252, Percent_Identity=26.984126984127, Blast_Score=106, Evalue=5e-24,
Organism=Drosophila melanogaster, GI18860087, Length=261, Percent_Identity=23.7547892720307, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): IF2A_METKA (Q8TY86)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613704.1
- ProteinModelPortal:   Q8TY86
- SMR:   Q8TY86
- GeneID:   1477722
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0419
- NMPDR:   fig|190192.1.peg.417
- HOGENOM:   HBG496806
- OMA:   EYPGKEG
- ProtClustDB:   PRK03987
- BioCyc:   MKAN190192:MK0419-MONOMER
- HAMAP:   MF_00231
- InterPro:   IPR011488
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR022964
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00316

Pfam domain/function: PF07541 EIF_2_alpha; PF00575 S1; SSF50249 Nucleic_acid_OB

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 30401; Mature: 30269

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRKLRDLPEEGEIVMATVERVEDHGAFVTLDEYPGVDGYIHISEVASGWVKNIRDYVKE
CCCCCCCCCCCCCEEHHHHHHHHHCCCEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHH
GQKVVAKVIRVNPKRKYANLSLRKVTDHQRKEKLKEWKREQRAEKLLEMAAEELGKDLDE
HHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYEEAGYKLIEEYGSLYDALERAAAEEGPEPLLKAGVPEEWAEKLAELAIENIEPGRVKI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEE
EAYVDLTCPAPNGVEIIREALEKIEEFQQGDVKMEVQYVGAPRYRITVDAPDYRTAEKMV
EEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECCCCCHHHHHHH
RKAAQAAIDHVEEHGGEGEFHREIEEG
HHHHHHHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure 
PRKLRDLPEEGEIVMATVERVEDHGAFVTLDEYPGVDGYIHISEVASGWVKNIRDYVKE
CCCCCCCCCCCCEEHHHHHHHHHCCCEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHH
GQKVVAKVIRVNPKRKYANLSLRKVTDHQRKEKLKEWKREQRAEKLLEMAAEELGKDLDE
HHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYEEAGYKLIEEYGSLYDALERAAAEEGPEPLLKAGVPEEWAEKLAELAIENIEPGRVKI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEE
EAYVDLTCPAPNGVEIIREALEKIEEFQQGDVKMEVQYVGAPRYRITVDAPDYRTAEKMV
EEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECCCCCHHHHHHH
RKAAQAAIDHVEEHGGEGEFHREIEEG
HHHHHHHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014