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The map label for this gene is GlnA [H]

Identifier: 20093776

GI number: 20093776

Start: 329455

End: 330930

Strand: Reverse

Name: GlnA [H]

Synonym: MK0338

Alternate gene names: 20093776

Gene position: 330930-329455 (Counterclockwise)

Preceding gene: 20093778

Following gene: 20093775

Centisome position: 19.52

GC content: 60.77

Gene sequence:

>1476_bases
ATGCCTCGGAGGTTGCAAAACGTCTATAAAGTCCCTGGGCGGGGCCGCCACCAGCTCAGGATTGCCGGAAAATCCATTCC
GGGGGTGTCGCCTTTGGCCGACGAGGTATACACGGTCGATTTCAAGAAGCGTCAGAAGGAGAACGAGGAGAAGGTCGAGA
AAGTGATGGAACAGGTTGAGAGTAAGGACGTCAAGTTCGTCAGGCTTCAGTTCGTCGACATCCACGGGTTCGTTAAGAAC
TTCGCGATCCCCGCGGAGCGCCTCGAGGACGCGCTGGTCGAGGGTGTCCTCTTCGACGGTTCGTCCATCGATGGCTTCGT
CGACATCGAGGAGTCGGACATGATCGCGATGCCCGACCCGGACACCTTCGCCGTGCTGCCGTGGAGGCCGAGGGAGGGTA
AGGTCGCGAGGATAATTTGCGACGTTTACTGGCCGGAGGGCAAGCCGTTCGAGGGCTGCCCGAGACTCAACCTGAAGCGC
GTGATGAACGAGCTCGCCGAGAAGGGCTACATGATGTACTGCGGTCCCGAGTGTGAGTTCTACCTGCTCAAGCAGGACCC
GGAGACGGGTGAAGTGGAGGCCCACGACGAGGGTGGCTACTTTGACTTCTACCCGCTGGACCGCGCCGAGGACATCCGGC
GTGAGATCATCTTCGCGATGGAGGAATTCGGTCTCGAGGTCGAGATGAGCCATCACGAGGTCGGTCCGGGTCAGCACGAG
ATCGACTTCAAGTTCGCGGACGCCGTGACCACGGCCGACAACGTCATCTCGTTCAAGCAGATCGTGAAGGCCATCGCCAG
GAAGCACGGTCTGATCGCGACGTTCATGCCGAAGCCGATCTACGGCGAGAACGGCAACGGAATGCACGTGCACCAGTCGC
TGTGGGATCCCGAAGGTAAGGAGAACCTGTTCTACGACCCGGATGCCGAGGACCAGTATTACTTGTCTGACACCGCCCTG
TACTACATCGGCGGTATCATGGAGCACGCCCACGCGCTGTGTGCCGTCTGCAACCCGCTCGTGAACTCGTACAAGCGACT
CGTGCCGGGTTACGAGGCTCCGGTGTACATCGCGTGGAGCCCGAAGAACAGGAGCGCATTCATCCGTGTACCGGCTGCGC
GGGGTAAGGCCACGCGAATCGAGTACCGCTCGCCGGACCCGGCATGCAACCCGTACCTAGCCTTCGCCGCGATGGCCGTC
GCGGGTGTCGACGGCATCGAGAACAAGATCGACCCGGGCGACCCGGTGAACGAGAACCTCTACGAGATGAGCGAGGAGGA
GCGGAAGGAACTCGGCATCGAGCACCTACCGGAGAACCTCCGCGACGCCCTGGAGGCGTTCAAGGAGGACGAGGTCGTGC
AGAGCGCGTTCACGGACCACATCGTGGAGCAGTGGCTGGAGCTGAAGTGGGAGGAGTGGAGCGAGTACCGGGAGCGCGTC
ACCAAGTGGGAGATCAAGAAGTTCATCGTATACTAA

Upstream 100 bases:

>100_bases
ACGCGAATACCCCGACATCGGTAGCACAGGCGGGCGTAGCGAGTAGAATGGCGGAGATTAAGGGTATCAGGGTTCTAGGG
AACATGTTCACCCCCCTCGA

Downstream 100 bases:

>100_bases
GCCCGGAACCTTCTTTTCCTACCCACCTTATCGTTACAGGGGTTGAAACGAAATGAACCGGCTGGACGTGATGATCCTCA
AGATACTCGCCGAGGCGGAC

Product: glutamine synthase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MPRRLQNVYKVPGRGRHQLRIAGKSIPGVSPLADEVYTVDFKKRQKENEEKVEKVMEQVESKDVKFVRLQFVDIHGFVKN
FAIPAERLEDALVEGVLFDGSSIDGFVDIEESDMIAMPDPDTFAVLPWRPREGKVARIICDVYWPEGKPFEGCPRLNLKR
VMNELAEKGYMMYCGPECEFYLLKQDPETGEVEAHDEGGYFDFYPLDRAEDIRREIIFAMEEFGLEVEMSHHEVGPGQHE
IDFKFADAVTTADNVISFKQIVKAIARKHGLIATFMPKPIYGENGNGMHVHQSLWDPEGKENLFYDPDAEDQYYLSDTAL
YYIGGIMEHAHALCAVCNPLVNSYKRLVPGYEAPVYIAWSPKNRSAFIRVPAARGKATRIEYRSPDPACNPYLAFAAMAV
AGVDGIENKIDPGDPVNENLYEMSEEERKELGIEHLPENLRDALEAFKEDEVVQSAFTDHIVEQWLELKWEEWSEYRERV
TKWEIKKFIVY

Sequences:

>Translated_491_residues
MPRRLQNVYKVPGRGRHQLRIAGKSIPGVSPLADEVYTVDFKKRQKENEEKVEKVMEQVESKDVKFVRLQFVDIHGFVKN
FAIPAERLEDALVEGVLFDGSSIDGFVDIEESDMIAMPDPDTFAVLPWRPREGKVARIICDVYWPEGKPFEGCPRLNLKR
VMNELAEKGYMMYCGPECEFYLLKQDPETGEVEAHDEGGYFDFYPLDRAEDIRREIIFAMEEFGLEVEMSHHEVGPGQHE
IDFKFADAVTTADNVISFKQIVKAIARKHGLIATFMPKPIYGENGNGMHVHQSLWDPEGKENLFYDPDAEDQYYLSDTAL
YYIGGIMEHAHALCAVCNPLVNSYKRLVPGYEAPVYIAWSPKNRSAFIRVPAARGKATRIEYRSPDPACNPYLAFAAMAV
AGVDGIENKIDPGDPVNENLYEMSEEERKELGIEHLPENLRDALEAFKEDEVVQSAFTDHIVEQWLELKWEEWSEYRERV
TKWEIKKFIVY
>Mature_490_residues
PRRLQNVYKVPGRGRHQLRIAGKSIPGVSPLADEVYTVDFKKRQKENEEKVEKVMEQVESKDVKFVRLQFVDIHGFVKNF
AIPAERLEDALVEGVLFDGSSIDGFVDIEESDMIAMPDPDTFAVLPWRPREGKVARIICDVYWPEGKPFEGCPRLNLKRV
MNELAEKGYMMYCGPECEFYLLKQDPETGEVEAHDEGGYFDFYPLDRAEDIRREIIFAMEEFGLEVEMSHHEVGPGQHEI
DFKFADAVTTADNVISFKQIVKAIARKHGLIATFMPKPIYGENGNGMHVHQSLWDPEGKENLFYDPDAEDQYYLSDTALY
YIGGIMEHAHALCAVCNPLVNSYKRLVPGYEAPVYIAWSPKNRSAFIRVPAARGKATRIEYRSPDPACNPYLAFAAMAVA
GVDGIENKIDPGDPVNENLYEMSEEERKELGIEHLPENLRDALEAFKEDEVVQSAFTDHIVEQWLELKWEEWSEYRERVT
KWEIKKFIVY

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=448, Percent_Identity=25.6696428571429, Blast_Score=157, Evalue=3e-38,
Organism=Escherichia coli, GI1790301, Length=464, Percent_Identity=40.7327586206897, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI87081870, Length=468, Percent_Identity=26.0683760683761, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 56366; Mature: 56235

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRRLQNVYKVPGRGRHQLRIAGKSIPGVSPLADEVYTVDFKKRQKENEEKVEKVMEQVE
CCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHEEEECHHHHHCCCHHHHHHHHHHHH
SKDVKFVRLQFVDIHGFVKNFAIPAERLEDALVEGVLFDGSSIDGFVDIEESDMIAMPDP
CCCCEEEEEEEEEHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCEEEECCCCEEECCCC
DTFAVLPWRPREGKVARIICDVYWPEGKPFEGCPRLNLKRVMNELAEKGYMMYCGPECEF
CCEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCEE
YLLKQDPETGEVEAHDEGGYFDFYPLDRAEDIRREIIFAMEEFGLEVEMSHHEVGPGQHE
EEEECCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCE
IDFKFADAVTTADNVISFKQIVKAIARKHGLIATFMPKPIYGENGNGMHVHQSLWDPEGK
EEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEEHHHCCCCCC
ENLFYDPDAEDQYYLSDTALYYIGGIMEHAHALCAVCNPLVNSYKRLVPGYEAPVYIAWS
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
PKNRSAFIRVPAARGKATRIEYRSPDPACNPYLAFAAMAVAGVDGIENKIDPGDPVNENL
CCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
YEMSEEERKELGIEHLPENLRDALEAFKEDEVVQSAFTDHIVEQWLELKWEEWSEYRERV
HHHCHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
TKWEIKKFIVY
HHHHHHHHHCC
>Mature Secondary Structure 
PRRLQNVYKVPGRGRHQLRIAGKSIPGVSPLADEVYTVDFKKRQKENEEKVEKVMEQVE
CHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCHHEEEECHHHHHCCCHHHHHHHHHHHH
SKDVKFVRLQFVDIHGFVKNFAIPAERLEDALVEGVLFDGSSIDGFVDIEESDMIAMPDP
CCCCEEEEEEEEEHHHHHHHHCCCHHHHHHHHHHHEEECCCCCCCEEEECCCCEEECCCC
DTFAVLPWRPREGKVARIICDVYWPEGKPFEGCPRLNLKRVMNELAEKGYMMYCGPECEF
CCEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCEE
YLLKQDPETGEVEAHDEGGYFDFYPLDRAEDIRREIIFAMEEFGLEVEMSHHEVGPGQHE
EEEECCCCCCCEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCE
IDFKFADAVTTADNVISFKQIVKAIARKHGLIATFMPKPIYGENGNGMHVHQSLWDPEGK
EEEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCEEEEHHHCCCCCC
ENLFYDPDAEDQYYLSDTALYYIGGIMEHAHALCAVCNPLVNSYKRLVPGYEAPVYIAWS
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
PKNRSAFIRVPAARGKATRIEYRSPDPACNPYLAFAAMAVAGVDGIENKIDPGDPVNENL
CCCCCEEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHH
YEMSEEERKELGIEHLPENLRDALEAFKEDEVVQSAFTDHIVEQWLELKWEEWSEYRERV
HHHCHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
TKWEIKKFIVY
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]