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The map label for this gene is Fwd_F2 [H]

Identifier: 20093761

GI number: 20093761

Start: 315226

End: 315678

Strand: Direct

Name: Fwd_F2 [H]

Synonym: MK0323

Alternate gene names: 20093761

Gene position: 315226-315678 (Clockwise)

Preceding gene: 20093760

Following gene: 20093764

Centisome position: 18.6

GC content: 65.56

Gene sequence:

>453_bases
CTGCGAGCGGGCCTGTTCCGTGAACGCGATCACCGTCGAGACCCCTTCCGCCGTTGTGCGCAAGGGCGCCGACCCACTCG
ACATCGACTAGGAGACGTGCGTGCTGTGCGGTATCTGCGCCTTCGTGTGCCCGTACGACGCGATCACCTGCTCATCGACG
GTGAACCGGTAACTGAGCGAGGCGTCCCCAAGCTGCCCAAGAACGTAGAGGTCGACGAGGACCGGTGCGTGTACTGCGGC
GTGTGCATGCGCACCTGCCCTGTCGACGCCATCCAGGTCACCAAGCCCTACCAGGGTCACATCGAGATCGACGACGAGGA
GTGCGTCGGCTGCGGACTCTGCGTAGAGATCTGCCCGTGCAACGCCCTCGAGTTCGGGCGGGACGGCACCGCCGAGAAGA
CCCGCATCGTCGTCAACCTCGACCGCGTCCTGGGGCCGACCGAGAAGGAGTAG

Upstream 100 bases:

>100_bases
CCGTGAAGGAAGTCTCCCCGCTCACCATCCGAATCGTGCGCGAGGGCGCGAAGACGCGGGAGCTGGTCGTGAACCTCGAC
CGGTGCGCCGCCTGCGGACT

Downstream 100 bases:

>100_bases
GCGCCCTCCCCCACGTCCCCGGCCAATCGTTATGAATTACAAAACGATAGGTGGAGCTAAGGTGGGATGAGAACAGCGGT
GAGCACGGACCGATGAAGAG

Product: formylmethanofuran dehydrogenase subunit F, ferredoxin containing

Products: CO2; methanofuran; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MRAGLFRERDHRRDPFRRCAQGRRPTRHRLGDVRAVRYLRLRVPVRRDHLLIDGEPVTERGVPKLPKNVEVDEDRCVYCG
VCMRTCPVDAIQVTKPYQGHIEIDDEECVGCGLCVEICPCNALEFGRDGTAEKTRIVVNLDRVLGPTEKE

Sequences:

>Translated_150_residues
MRAGLFRERDHRRDPFRRCAQGRRPTRHRLGDVRAVRYLRLRVPVRRDHLLIDGEPVTERGVPKLPKNVEVDEDRCVYCG
VCMRTCPVDAIQVTKPYQGHIEIDDEECVGCGLCVEICPCNALEFGRDGTAEKTRIVVNLDRVLGPTEKE
>Mature_150_residues
MRAGLFRERDHRRDPFRRCAQGRRPTRHRLGDVRAVRYLRLRVPVRRDHLLIDGEPVTERGVPKLPKNVEVDEDRCVYCG
VCMRTCPVDAIQVTKPYQGHIEIDDEECVGCGLCVEICPCNALEFGRDGTAEKTRIVVNLDRVLGPTEKE

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG1145

COG function: function code C; Ferredoxin

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 8 4Fe-4S ferredoxin-type domains [H]

Homologues:

None

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR016459 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.99.5

Molecular weight: Translated: 17210; Mature: 17210

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
6.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAGLFRERDHRRDPFRRCAQGRRPTRHRLGDVRAVRYLRLRVPVRRDHLLIDGEPVTER
CCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCEEEECCCCCCCC
GVPKLPKNVEVDEDRCVYCGVCMRTCPVDAIQVTKPYQGHIEIDDEECVGCGLCVEICPC
CCCCCCCCCCCCCCCEEEEEHHHHHCCCCEEEECCCCCCCEEECCCCCCCCCEEEEECCC
NALEFGRDGTAEKTRIVVNLDRVLGPTEKE
CCCCCCCCCCCCCEEEEEEEHHHCCCCCCC
>Mature Secondary Structure
MRAGLFRERDHRRDPFRRCAQGRRPTRHRLGDVRAVRYLRLRVPVRRDHLLIDGEPVTER
CCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHEECCCCCCEEEECCCCCCCC
GVPKLPKNVEVDEDRCVYCGVCMRTCPVDAIQVTKPYQGHIEIDDEECVGCGLCVEICPC
CCCCCCCCCCCCCCCEEEEEHHHHHCCCCEEEECCCCCCCEEECCCCCCCCCEEEEECCC
NALEFGRDGTAEKTRIVVNLDRVLGPTEKE
CCCCCCCCCCCCCEEEEEEEHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formylmethanofuran; H2O; acceptor

Specific reaction: formylmethanofuran + H2O + acceptor = CO2 + methanofuran + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]