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The map label for this gene is 20093669

Identifier: 20093669

GI number: 20093669

Start: 215254

End: 216432

Strand: Direct

Name: 20093669

Synonym: MK0229

Alternate gene names: NA

Gene position: 215254-216432 (Clockwise)

Preceding gene: 20093668

Following gene: 20093670

Centisome position: 12.7

GC content: 61.07

Gene sequence:

>1179_bases
TTGAACCTGGACCGTTATCGGAACATCGTCCGTGAGACGGAGCGGAAGTACATCAATGTGAACCCTATCCAACGAGGGGG
CGTGCTGACCCCGGAGGCTCGGAAAGCCCTCCTGGAGTTCGGAGATGGGTACAGTGTATGCGACTTCTGCGAGGGGTTGC
TCCATGAGATCGAGAAACCTCCCATCCGACAGTTTCACGAGGACTTAGCCGAGTTTCTCGGGATGGACGTTGTGCGGATC
ACCGCCGGTGCGAGGTACGCGAAGGAAGCCGTCATGAGCGCGCTGTGCGAGGAGGGAGACGTAGTCGTCGCGGACTCGCT
CGCGCACTACACCACGTTCGTCGCCGCGGAGAAGGCGGGAGCCACGGTCCGCGAGGTCCCGAACACCGGTCACCCGGAGT
ACAAGGTGAAGGTCGACGAGTACGCCCGGGTCATCGACGAGGTTGAGGACGAGCGGGGAGACCCACCCGCGTTGGCGTTA
CTCACACACGTCGATAGTGAGTACGGGAACCTCGCGGATGCGGAGAAGTTCGTGAAGATATGCCGCAAGAAGGGTGTTCC
GGCGCTGCTCAACTGCGCCTACACCATGGGTCGCATGGACCTGTCCAACCTGAGCCCGAAGCCCGACTTCATGGTCGGTA
GCGGTCATAAGGGCATGGCCGCGTGCGCACCGTGCGGCGTACTCGCGATGCGGGAGGAATGGGAAGAGGAAGTGCTCAGG
GGATCCTCACTGCGCGGAGACGTCTCCGGTCGGGAGTGGCCACACAAGGAGGTCGAAATGCTCGGATGTACCGTGATGGG
AGCGCCGATCGTCACTATGATGGCCAGCTTCCCCCACGTCGTGGAGCGGGTGAAACGTTGGAAGGAGGAGGTGCGAAAGA
CGCGGTGGTTCGTGAAGGAGATGGAAAGGATAGAGGGCGTACGACAACTCGGTGAGCGACCGAAGCGGCACGATCTGGTG
AAGTTTGAGACTCCGGGCTTCCACGAGGTGGCGGAAGACCATCCCAGGCGCGGCTACTTCCTGTACGAGGAGTTGAAAAA
GCGTGGAGTCATCGGTATCCAACCCGGACAGACCGAGACCATCAAGGCCAGCGTATACGGTCTGACGGACGAGCAGGTAG
AGCACGTCGTGCGAGCGTTCCACGAGATCGCGGAGGAGTACGGGCTAGAAGTCTCCTGA

Upstream 100 bases:

>100_bases
AGGTCAAGGCCGTGACCTACCACAACATGAAGGTCGAGCGCGAAGACGGACGGTGGGTAGCCGAGTACGTGGTCGACTTG
TGAAGGGGGCAGGTCGATCT

Downstream 100 bases:

>100_bases
GTCGTTCGGTCGGACCGACGGGGATCTCCCGCGGAAGTATTGAGGGACAGGAGCCCTAGATAGCGCGCCGGAGCGTGACT
GTGACGTGTGCGGAGTGAAG

Product: Sep-tRNA:Cys-tRNA synthetase

Products: NA

Alternate protein names: Sep-tRNA:Cys-tRNA synthase; SepCysS

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MNLDRYRNIVRETERKYINVNPIQRGGVLTPEARKALLEFGDGYSVCDFCEGLLHEIEKPPIRQFHEDLAEFLGMDVVRI
TAGARYAKEAVMSALCEEGDVVVADSLAHYTTFVAAEKAGATVREVPNTGHPEYKVKVDEYARVIDEVEDERGDPPALAL
LTHVDSEYGNLADAEKFVKICRKKGVPALLNCAYTMGRMDLSNLSPKPDFMVGSGHKGMAACAPCGVLAMREEWEEEVLR
GSSLRGDVSGREWPHKEVEMLGCTVMGAPIVTMMASFPHVVERVKRWKEEVRKTRWFVKEMERIEGVRQLGERPKRHDLV
KFETPGFHEVAEDHPRRGYFLYEELKKRGVIGIQPGQTETIKASVYGLTDEQVEHVVRAFHEIAEEYGLEVS

Sequences:

>Translated_392_residues
MNLDRYRNIVRETERKYINVNPIQRGGVLTPEARKALLEFGDGYSVCDFCEGLLHEIEKPPIRQFHEDLAEFLGMDVVRI
TAGARYAKEAVMSALCEEGDVVVADSLAHYTTFVAAEKAGATVREVPNTGHPEYKVKVDEYARVIDEVEDERGDPPALAL
LTHVDSEYGNLADAEKFVKICRKKGVPALLNCAYTMGRMDLSNLSPKPDFMVGSGHKGMAACAPCGVLAMREEWEEEVLR
GSSLRGDVSGREWPHKEVEMLGCTVMGAPIVTMMASFPHVVERVKRWKEEVRKTRWFVKEMERIEGVRQLGERPKRHDLV
KFETPGFHEVAEDHPRRGYFLYEELKKRGVIGIQPGQTETIKASVYGLTDEQVEHVVRAFHEIAEEYGLEVS
>Mature_392_residues
MNLDRYRNIVRETERKYINVNPIQRGGVLTPEARKALLEFGDGYSVCDFCEGLLHEIEKPPIRQFHEDLAEFLGMDVVRI
TAGARYAKEAVMSALCEEGDVVVADSLAHYTTFVAAEKAGATVREVPNTGHPEYKVKVDEYARVIDEVEDERGDPPALAL
LTHVDSEYGNLADAEKFVKICRKKGVPALLNCAYTMGRMDLSNLSPKPDFMVGSGHKGMAACAPCGVLAMREEWEEEVLR
GSSLRGDVSGREWPHKEVEMLGCTVMGAPIVTMMASFPHVVERVKRWKEEVRKTRWFVKEMERIEGVRQLGERPKRHDLV
KFETPGFHEVAEDHPRRGYFLYEELKKRGVIGIQPGQTETIKASVYGLTDEQVEHVVRAFHEIAEEYGLEVS

Specific function: Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys))

COG id: COG1103

COG function: function code R; Archaea-specific pyridoxal phosphate-dependent enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SepCysS family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPSS_METKA (Q8TYR3)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613516.1
- ProteinModelPortal:   Q8TYR3
- SMR:   Q8TYR3
- GeneID:   1477532
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0229
- NMPDR:   fig|190192.1.peg.229
- HOGENOM:   HBG539014
- OMA:   AHYTEFL
- ProtClustDB:   PRK09331
- BioCyc:   MKAN190192:MK0229-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_01675
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR013375
- InterPro:   IPR008829
- Gene3D:   G3DSA:3.40.640.10
- TIGRFAMs:   TIGR02539

Pfam domain/function: PF05889 SLA_LP_auto_ag; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.5.1.73

Molecular weight: Translated: 44252; Mature: 44252

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: BINDING 85-85 BINDING 109-109 BINDING 110-110 BINDING 191-191 BINDING 283-283 BINDING 292-292

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDRYRNIVRETERKYINVNPIQRGGVLTPEARKALLEFGDGYSVCDFCEGLLHEIEKP
CCHHHHHHHHHHHHHCEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
PIRQFHEDLAEFLGMDVVRITAGARYAKEAVMSALCEEGDVVVADSLAHYTTFVAAEKAG
HHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCC
ATVREVPNTGHPEYKVKVDEYARVIDEVEDERGDPPALALLTHVDSEYGNLADAEKFVKI
CHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
CRKKGVPALLNCAYTMGRMDLSNLSPKPDFMVGSGHKGMAACAPCGVLAMREEWEEEVLR
HHHCCCCHHHHHHHHHCCCCHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
GSSLRGDVSGREWPHKEVEMLGCTVMGAPIVTMMASFPHVVERVKRWKEEVRKTRWFVKE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MERIEGVRQLGERPKRHDLVKFETPGFHEVAEDHPRRGYFLYEELKKRGVIGIQPGQTET
HHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHCCCCCCCHHHHHHHHCCCEEECCCCCCE
IKASVYGLTDEQVEHVVRAFHEIAEEYGLEVS
EEEEEECCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNLDRYRNIVRETERKYINVNPIQRGGVLTPEARKALLEFGDGYSVCDFCEGLLHEIEKP
CCHHHHHHHHHHHHHCEECCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
PIRQFHEDLAEFLGMDVVRITAGARYAKEAVMSALCEEGDVVVADSLAHYTTFVAAEKAG
HHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCC
ATVREVPNTGHPEYKVKVDEYARVIDEVEDERGDPPALALLTHVDSEYGNLADAEKFVKI
CHHHHCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
CRKKGVPALLNCAYTMGRMDLSNLSPKPDFMVGSGHKGMAACAPCGVLAMREEWEEEVLR
HHHCCCCHHHHHHHHHCCCCHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
GSSLRGDVSGREWPHKEVEMLGCTVMGAPIVTMMASFPHVVERVKRWKEEVRKTRWFVKE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MERIEGVRQLGERPKRHDLVKFETPGFHEVAEDHPRRGYFLYEELKKRGVIGIQPGQTET
HHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHCCCCCCCHHHHHHHHCCCEEECCCCCCE
IKASVYGLTDEQVEHVVRAFHEIAEEYGLEVS
EEEEEECCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014