Click here to switch to the map view.

The map label for this gene is HemB [H]

Identifier: 20093638

GI number: 20093638

Start: 191625

End: 192632

Strand: Reverse

Name: HemB [H]

Synonym: MK0198

Alternate gene names: 20093638

Gene position: 192632-191625 (Counterclockwise)

Preceding gene: 20093640

Following gene: 20093637

Centisome position: 11.36

GC content: 60.91

Gene sequence:

>1008_bases
TTGAAGCACTCCCCGTGGGGGAGAGACGGATTGTATCCGACCGTCCGCATGCGCAGGTATCGTAAGTCCGAGGCGATCCG
TGATCTCGTCGCCGAAACGGAAGTGCGCCCGGACGATCTAATCTACCCGATCTTCGTACGTGAGGACGGGAAGACCCACG
AAATTCCCTCAATGCCCGGTCAACGCTACCACTCCGTGGAAACTGCCGTCGAGACAGTTAGAGAACTCCTGGACCTGGGC
CTACGGGCGTTCATACTCTTCGGGATCCCGAGAGAAAAGGACCCTGAAGGTCGAGTCGCCGCGGATCCCGAGGGTATAGT
CCAAAGGACCGTGCGGGCGCTCAAGGAGGAGTACGGCGACGACATTGTCGTGGTCACGGACGTTTGTCTCTGCCAGTACA
CTACCCACGGTCATTGCGGCCTCGTCGACGAGGATACGGGTAAGGTGCTGAACGACCCCACCCTGGAGGTCTTGGCGGAG
GTGGCTCTTTCCCACGCCGAAGCCGGGGCCGACATAGTCGCCCCCTCCGATATGATGGACGGTCGGGTGAAGGTGATTAG
AGAGGCGCTTGAGAGCGAGGGTTTCGACGATGTCCTGATCATGTCTTATGCGGCCAAGTATCACAGCGCGTTCTACGGCC
CGTTCCGGGACGCAGCGGACTCGGCACCGGAGTTCGGGGATCGGAGCACTTACCAGATGGATCCTCGGTGCTTCCGCCAG
GCGATCCGCGAGCTGGAGCTAGACGCCGAGGAGGGAGCGGACATCTTAATGATTAAGCCGGCGATGCCCTACCTGGACGT
GGTGCGCGAGGCTCGGCGGAGGTTCGACCACCCCATCGCGGCCTATCAGGTTAGCGGTGAATACGCGATGATCAAGGCGG
CGGCCGAGGCGGGGTACGTGGATTACCGGACCGCTGTGCTGGAGTCCCTAACGTGCATCAAGAGAGCTGGGGCGGATTTG
ATACTCACGTACTTCGCTCCGGAGGTGGTGCGGTGGCTGAAATCGTAG

Upstream 100 bases:

>100_bases
CAGCCGCGCCCATCCCTCCGACCACTTTATGAACGCCTCTTTGAACACGTCTACCACACCGAACGCCGTTTCACGGGACT
CCTTCCTCCCGCGTATCAAC

Downstream 100 bases:

>100_bases
TGCGGTTCGATCATCGCGTGATCGAGAAGATCCCGAGACGGCCCCACCCGGTCTTCGAGCTCATCGGTTTCATCTTCAGT
GGTTGGGTTTTATTCCCCTC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKHSPWGRDGLYPTVRMRRYRKSEAIRDLVAETEVRPDDLIYPIFVREDGKTHEIPSMPGQRYHSVETAVETVRELLDLG
LRAFILFGIPREKDPEGRVAADPEGIVQRTVRALKEEYGDDIVVVTDVCLCQYTTHGHCGLVDEDTGKVLNDPTLEVLAE
VALSHAEAGADIVAPSDMMDGRVKVIREALESEGFDDVLIMSYAAKYHSAFYGPFRDAADSAPEFGDRSTYQMDPRCFRQ
AIRELELDAEEGADILMIKPAMPYLDVVREARRRFDHPIAAYQVSGEYAMIKAAAEAGYVDYRTAVLESLTCIKRAGADL
ILTYFAPEVVRWLKS

Sequences:

>Translated_335_residues
MKHSPWGRDGLYPTVRMRRYRKSEAIRDLVAETEVRPDDLIYPIFVREDGKTHEIPSMPGQRYHSVETAVETVRELLDLG
LRAFILFGIPREKDPEGRVAADPEGIVQRTVRALKEEYGDDIVVVTDVCLCQYTTHGHCGLVDEDTGKVLNDPTLEVLAE
VALSHAEAGADIVAPSDMMDGRVKVIREALESEGFDDVLIMSYAAKYHSAFYGPFRDAADSAPEFGDRSTYQMDPRCFRQ
AIRELELDAEEGADILMIKPAMPYLDVVREARRRFDHPIAAYQVSGEYAMIKAAAEAGYVDYRTAVLESLTCIKRAGADL
ILTYFAPEVVRWLKS
>Mature_335_residues
MKHSPWGRDGLYPTVRMRRYRKSEAIRDLVAETEVRPDDLIYPIFVREDGKTHEIPSMPGQRYHSVETAVETVRELLDLG
LRAFILFGIPREKDPEGRVAADPEGIVQRTVRALKEEYGDDIVVVTDVCLCQYTTHGHCGLVDEDTGKVLNDPTLEVLAE
VALSHAEAGADIVAPSDMMDGRVKVIREALESEGFDDVLIMSYAAKYHSAFYGPFRDAADSAPEFGDRSTYQMDPRCFRQ
AIRELELDAEEGADILMIKPAMPYLDVVREARRRFDHPIAAYQVSGEYAMIKAAAEAGYVDYRTAVLESLTCIKRAGADL
ILTYFAPEVVRWLKS

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=310, Percent_Identity=46.7741935483871, Blast_Score=280, Evalue=2e-75,
Organism=Escherichia coli, GI87081728, Length=312, Percent_Identity=49.3589743589744, Blast_Score=271, Evalue=4e-74,
Organism=Saccharomyces cerevisiae, GI6321398, Length=314, Percent_Identity=43.9490445859873, Blast_Score=245, Evalue=7e-66,
Organism=Drosophila melanogaster, GI21358291, Length=299, Percent_Identity=44.1471571906354, Blast_Score=252, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 37654; Mature: 37654

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHSPWGRDGLYPTVRMRRYRKSEAIRDLVAETEVRPDDLIYPIFVREDGKTHEIPSMPG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCC
QRYHSVETAVETVRELLDLGLRAFILFGIPREKDPEGRVAADPEGIVQRTVRALKEEYGD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
DIVVVTDVCLCQYTTHGHCGLVDEDTGKVLNDPTLEVLAEVALSHAEAGADIVAPSDMMD
CEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCHHHC
GRVKVIREALESEGFDDVLIMSYAAKYHSAFYGPFRDAADSAPEFGDRSTYQMDPRCFRQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHHHHH
AIRELELDAEEGADILMIKPAMPYLDVVREARRRFDHPIAAYQVSGEYAMIKAAAEAGYV
HHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEHHHHCCCH
DYRTAVLESLTCIKRAGADLILTYFAPEVVRWLKS
HHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHC
>Mature Secondary Structure
MKHSPWGRDGLYPTVRMRRYRKSEAIRDLVAETEVRPDDLIYPIFVREDGKTHEIPSMPG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCC
QRYHSVETAVETVRELLDLGLRAFILFGIPREKDPEGRVAADPEGIVQRTVRALKEEYGD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
DIVVVTDVCLCQYTTHGHCGLVDEDTGKVLNDPTLEVLAEVALSHAEAGADIVAPSDMMD
CEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCHHHC
GRVKVIREALESEGFDDVLIMSYAAKYHSAFYGPFRDAADSAPEFGDRSTYQMDPRCFRQ
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCHHHHHH
AIRELELDAEEGADILMIKPAMPYLDVVREARRRFDHPIAAYQVSGEYAMIKAAAEAGYV
HHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCEEEEEHHHHCCCH
DYRTAVLESLTCIKRAGADLILTYFAPEVVRWLKS
HHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1398086 [H]