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The map label for this gene is TRM1_1

Identifier: 20093594

GI number: 20093594

Start: 153397

End: 154548

Strand: Reverse

Name: TRM1_1

Synonym: MK0154

Alternate gene names: 20093594

Gene position: 154548-153397 (Counterclockwise)

Preceding gene: 20093595

Following gene: 20093593

Centisome position: 9.12

GC content: 62.15

Gene sequence:

>1152_bases
ATGGAGCTTGAGATAATCACCGAGGGCCGCACGCCGCTGAAAGTCCCGAAGACCCGCGGGCAGCCCTCCGCCAGAGACCC
AGTGTTCTACAACCCCGCAATGCAACTGAGCCGCGATTTAACGGTATCCTCGCTGGTGCAATACGGCCCGAAGATCGTGT
GTGATCCGCTGGCGGGAGTAGGCGCTCGCGGGATCCGCATCGCCGTCGAGTTGAGTCCTGAGGTGGTCGTCCTCAACGAT
CTCAATCCCCGCGCGGTCGAGCTGATCGAGGAGAACGTCCGGTTGAACGACGTCGAGGACGTCTGCAGGATCGAGAACCG
TGATGCGAACGCCTTGATGCACGAAGATGAGCTGGCAGGTCGTTTCGATTACGTCGACATCGATCCGTTCGGGCCTCCGG
TCCCGTTCTTGGATGCCGCCGTTAGAACGGTCCGGAACCGTGGCGTGGTCGGGATCTCCGCCACCGACGTCTCGGCCCTC
GCAGGGCGTTACCCGCGCTCGGCCCGGAGGAAGTATTGGGTCGAGGTTGAGCGCGTCGAGTTCTACCAGGAAGTGGCCAT
CCGCGCGCTGATTTCGTACATCGTCCGAACGTGTGCGAAGTACGACCTCGCGTTCGAACCACATATCGCGTTCTTCCAGC
GTCATCATGTGCGCGTAATCGGCGAGATCCGAAGAGGAGCCCGCCGAGCCGATCGGGCGTTAAAACGGCTCGGTTACCTT
CTGCACTGTCGCGAGTGTGGGTACACTTCGGAGCGCGAGTTCGATCGTGAGTGCCCGCGCTGCGGGTCAGGGAGCGTAGT
AAGGTTGGGACCCCTGTGGTTGCCCGACTTCGCGGACCGGGAGCGGGCCGAACGTGCGGCTTCGGACGCCCGCGAACTCG
GGCTCGAAGAGGCTGCGGAACTCCTGGAGACTGTGGCGAAGGAGACCGGGACGAATCCTTGGGCTTACGATATCCACCGA
TGGGCGTCGAGGTTGGGCTTATCCAGGGTGCCGAGTCTGACCTCGGTACTTGAGGGGCTCCGTGAGGAGGGATTCAACGC
GGTGCGTCCACACTATTCAAAGAGAGCCGTTGTGAAAACCGACGCGAGTCCGGAGGAGTTCGAAGCCGTCCTCACAGAGG
TGGCGGGGGACAGCGGATGCTTACACAGGTAG

Upstream 100 bases:

>100_bases
GTACGCGTCCGGTTCGAGCGTGGTCTCCCGGGCCAGGCCCTGGGTACCGAGGTGACACTGAAGTGAGCGCACCCTCAGGG
ACAGGGCCAGGGACAGGGCG

Downstream 100 bases:

>100_bases
CGTTGGATCTTACATCCCTGCCCAAAGCCATCGAAATAGCCGAAGCGAGCGTGGAGGCCGGAATCCACGTACTTGAGGTC
GGAACACCGCTCATCAAGGC

Product: N(2),N(2)-dimethylguanosine tRNA methyltransferase

Products: NA

Alternate protein names: tRNA 2,2-dimethylguanosine-26 methyltransferase; tRNA(guanine-26,N(2)-N(2)) methyltransferase; tRNA(m(2,2)G26)dimethyltransferase

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MELEIITEGRTPLKVPKTRGQPSARDPVFYNPAMQLSRDLTVSSLVQYGPKIVCDPLAGVGARGIRIAVELSPEVVVLND
LNPRAVELIEENVRLNDVEDVCRIENRDANALMHEDELAGRFDYVDIDPFGPPVPFLDAAVRTVRNRGVVGISATDVSAL
AGRYPRSARRKYWVEVERVEFYQEVAIRALISYIVRTCAKYDLAFEPHIAFFQRHHVRVIGEIRRGARRADRALKRLGYL
LHCRECGYTSEREFDRECPRCGSGSVVRLGPLWLPDFADRERAERAASDARELGLEEAAELLETVAKETGTNPWAYDIHR
WASRLGLSRVPSLTSVLEGLREEGFNAVRPHYSKRAVVKTDASPEEFEAVLTEVAGDSGCLHR

Sequences:

>Translated_383_residues
MELEIITEGRTPLKVPKTRGQPSARDPVFYNPAMQLSRDLTVSSLVQYGPKIVCDPLAGVGARGIRIAVELSPEVVVLND
LNPRAVELIEENVRLNDVEDVCRIENRDANALMHEDELAGRFDYVDIDPFGPPVPFLDAAVRTVRNRGVVGISATDVSAL
AGRYPRSARRKYWVEVERVEFYQEVAIRALISYIVRTCAKYDLAFEPHIAFFQRHHVRVIGEIRRGARRADRALKRLGYL
LHCRECGYTSEREFDRECPRCGSGSVVRLGPLWLPDFADRERAERAASDARELGLEEAAELLETVAKETGTNPWAYDIHR
WASRLGLSRVPSLTSVLEGLREEGFNAVRPHYSKRAVVKTDASPEEFEAVLTEVAGDSGCLHR
>Mature_383_residues
MELEIITEGRTPLKVPKTRGQPSARDPVFYNPAMQLSRDLTVSSLVQYGPKIVCDPLAGVGARGIRIAVELSPEVVVLND
LNPRAVELIEENVRLNDVEDVCRIENRDANALMHEDELAGRFDYVDIDPFGPPVPFLDAAVRTVRNRGVVGISATDVSAL
AGRYPRSARRKYWVEVERVEFYQEVAIRALISYIVRTCAKYDLAFEPHIAFFQRHHVRVIGEIRRGARRADRALKRLGYL
LHCRECGYTSEREFDRECPRCGSGSVVRLGPLWLPDFADRERAERAASDARELGLEEAAELLETVAKETGTNPWAYDIHR
WASRLGLSRVPSLTSVLEGLREEGFNAVRPHYSKRAVVKTDASPEEFEAVLTEVAGDSGCLHR

Specific function: Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups

COG id: COG1867

COG function: function code J; N2,N2-dimethylguanosine tRNA methyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TRM1 family

Homologues:

Organism=Homo sapiens, GI163792194, Length=357, Percent_Identity=29.9719887955182, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI8923219, Length=338, Percent_Identity=27.810650887574, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI209862871, Length=338, Percent_Identity=27.810650887574, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI216548445, Length=317, Percent_Identity=29.6529968454259, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17564890, Length=442, Percent_Identity=26.0180995475113, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6320325, Length=451, Percent_Identity=25.9423503325942, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19921190, Length=415, Percent_Identity=23.855421686747, Blast_Score=111, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRM1_METKA (Q8TYY7)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613441.1
- ProteinModelPortal:   Q8TYY7
- SMR:   Q8TYY7
- GeneID:   1477457
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0154
- NMPDR:   fig|190192.1.peg.154
- HOGENOM:   HBG577872
- OMA:   DAPVFYN
- ProtClustDB:   PRK04338
- BioCyc:   MKAN190192:MK0154-MONOMER
- HAMAP:   MF_00290
- InterPro:   IPR022923
- InterPro:   IPR004039
- InterPro:   IPR002905
- Gene3D:   G3DSA:2.20.28.10
- PANTHER:   PTHR10631
- TIGRFAMs:   TIGR00308

Pfam domain/function: PF02005 TRM

EC number: =2.1.1.32

Molecular weight: Translated: 43035; Mature: 43035

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELEIITEGRTPLKVPKTRGQPSARDPVFYNPAMQLSRDLTVSSLVQYGPKIVCDPLAGV
CCEEEEECCCCCCCCCCCCCCCCCCCCEEECHHHHHHHCCHHHHHHHCCCCEEECCCCCC
GARGIRIAVELSPEVVVLNDLNPRAVELIEENVRLNDVEDVCRIENRDANALMHEDELAG
CCCCEEEEEEECCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHCHHHHCC
RFDYVDIDPFGPPVPFLDAAVRTVRNRGVVGISATDVSALAGRYPRSARRKYWVEVERVE
CCCEEECCCCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHH
FYQEVAIRALISYIVRTCAKYDLAFEPHIAFFQRHHVRVIGEIRRGARRADRALKRLGYL
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHCRECGYTSEREFDRECPRCGSGSVVRLGPLWLPDFADRERAERAASDARELGLEEAAE
HHHHHCCCCCCHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
LLETVAKETGTNPWAYDIHRWASRLGLSRVPSLTSVLEGLREEGFNAVRPHYSKRAVVKT
HHHHHHHHHCCCCCHHHHHHHHHHHCHHCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEC
DASPEEFEAVLTEVAGDSGCLHR
CCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MELEIITEGRTPLKVPKTRGQPSARDPVFYNPAMQLSRDLTVSSLVQYGPKIVCDPLAGV
CCEEEEECCCCCCCCCCCCCCCCCCCCEEECHHHHHHHCCHHHHHHHCCCCEEECCCCCC
GARGIRIAVELSPEVVVLNDLNPRAVELIEENVRLNDVEDVCRIENRDANALMHEDELAG
CCCCEEEEEEECCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHCHHHHCC
RFDYVDIDPFGPPVPFLDAAVRTVRNRGVVGISATDVSALAGRYPRSARRKYWVEVERVE
CCCEEECCCCCCCCHHHHHHHHHHHHCCEEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHH
FYQEVAIRALISYIVRTCAKYDLAFEPHIAFFQRHHVRVIGEIRRGARRADRALKRLGYL
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHCRECGYTSEREFDRECPRCGSGSVVRLGPLWLPDFADRERAERAASDARELGLEEAAE
HHHHHCCCCCCHHHHHHCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
LLETVAKETGTNPWAYDIHRWASRLGLSRVPSLTSVLEGLREEGFNAVRPHYSKRAVVKT
HHHHHHHHHCCCCCHHHHHHHHHHHCHHCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEC
DASPEEFEAVLTEVAGDSGCLHR
CCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014