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The map label for this gene is NadA

Identifier: 20093513

GI number: 20093513

Start: 68133

End: 69011

Strand: Reverse

Name: NadA

Synonym: MK0073

Alternate gene names: 20093513

Gene position: 69011-68133 (Counterclockwise)

Preceding gene: 20093514

Following gene: 20093512

Centisome position: 4.07

GC content: 59.5

Gene sequence:

>879_bases
GTGGAGACGTTCGTGCTGGCTCACAATTACCAGCGGCCCGACGTGCAGCTTATGGCGGACTCCGTCGGGGATTCGCTGGA
GCTGGCCTTGGAGGCCAGGGAGATCGACGCTGATCGTATCGTAATGTGCGGAGTGGACTTCATGGCGGAGGTTGTGAAGG
CACTCAACCCCGACCGTGAAGTCGTCGTGCCCGATCACCGCGCTGCGTGCGGCATGGCCATGCGGCTCAGGGCGAAAGAA
CTGCGAGAGTTTCGCCGCGAGCATCCGGACGCCGCCGTGGTCGTGTACGTCAACACGAGCGCCGAAGTGAAGGCTGAGGC
CGACGTGATGTGCACTAGTGCTAACGCCGTCGAAGTCGTGTCCTCACTCCCCGAGGATAAGATCATCTTCGTTCCCGACG
GTAACCTAGCGGCCTGGGTGCAGAAACACGTGCCGGACAAGGAGGTAATCCCGTTCCCCGAGCACGGTTGCTGCCCCGTC
CATCACTCGCTGTCCCCTTCCGACCTACGCGAACTGTGTTCTCAACATCCGGATGCCGCCGTCGTGGTACACCCGGAATG
CCCTCTTGAAGTATGCGCGATGGCGGACTTCGTCGGCTCCACGTCCCAAATACGTCAATACTGTGAAAAGGAGGACGCCA
GTAAAATCGTAATGGGGACCGAGGAAGGTCTCGCCTTCAGGATACGTCGCGAAACGGGCACCGAAGTCATCGTTCCCGGC
CATATGGTGTGTCCCGATATGAAAATCAACACGGGGGAAAAAGTCGAAAGAGTACTCGAGGCCCGACACGTCCCGGAACC
GCTCAGAGTGGAGCTGGACCCCGACCTTATCTCTCAGGTCGAAGAAGTAGTCGAGGAGATGTTCAGGCTCACGAGGTGA

Upstream 100 bases:

>100_bases
TACCATCGACCCAGACCTCGTGTCCGAGGTGATGATCGAAATTCCGGAACGCGTATTCGGGGTCGAGTTCGAGCCGTTGG
GGTAGAGGGGGTAGGGCTCC

Downstream 100 bases:

>100_bases
ACCGGGATGATCGGTAGGATCTTCCGTCGAGAGAGTAAGGTCGTACGGGACCGGCTGATGAAGATAAGGCAAACGATCGA
CGCGACCGAAGTAGATCCAA

Product: quinolinate synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
METFVLAHNYQRPDVQLMADSVGDSLELALEAREIDADRIVMCGVDFMAEVVKALNPDREVVVPDHRAACGMAMRLRAKE
LREFRREHPDAAVVVYVNTSAEVKAEADVMCTSANAVEVVSSLPEDKIIFVPDGNLAAWVQKHVPDKEVIPFPEHGCCPV
HHSLSPSDLRELCSQHPDAAVVVHPECPLEVCAMADFVGSTSQIRQYCEKEDASKIVMGTEEGLAFRIRRETGTEVIVPG
HMVCPDMKINTGEKVERVLEARHVPEPLRVELDPDLISQVEEVVEEMFRLTR

Sequences:

>Translated_292_residues
METFVLAHNYQRPDVQLMADSVGDSLELALEAREIDADRIVMCGVDFMAEVVKALNPDREVVVPDHRAACGMAMRLRAKE
LREFRREHPDAAVVVYVNTSAEVKAEADVMCTSANAVEVVSSLPEDKIIFVPDGNLAAWVQKHVPDKEVIPFPEHGCCPV
HHSLSPSDLRELCSQHPDAAVVVHPECPLEVCAMADFVGSTSQIRQYCEKEDASKIVMGTEEGLAFRIRRETGTEVIVPG
HMVCPDMKINTGEKVERVLEARHVPEPLRVELDPDLISQVEEVVEEMFRLTR
>Mature_292_residues
METFVLAHNYQRPDVQLMADSVGDSLELALEAREIDADRIVMCGVDFMAEVVKALNPDREVVVPDHRAACGMAMRLRAKE
LREFRREHPDAAVVVYVNTSAEVKAEADVMCTSANAVEVVSSLPEDKIIFVPDGNLAAWVQKHVPDKEVIPFPEHGCCPV
HHSLSPSDLRELCSQHPDAAVVVHPECPLEVCAMADFVGSTSQIRQYCEKEDASKIVMGTEEGLAFRIRRETGTEVIVPG
HMVCPDMKINTGEKVERVLEARHVPEPLRVELDPDLISQVEEVVEEMFRLTR

Specific function: Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate

COG id: COG0379

COG function: function code H; Quinolinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the quinolinate synthase A family. Type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1786964, Length=288, Percent_Identity=32.9861111111111, Blast_Score=147, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADA_METKA (Q8TZ66)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613360.1
- ProteinModelPortal:   Q8TZ66
- SMR:   Q8TZ66
- GeneID:   1477376
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0073
- NMPDR:   fig|190192.1.peg.73
- HOGENOM:   HBG291157
- OMA:   CPVHEFL
- ProtClustDB:   PRK09375
- BioCyc:   MKAN190192:MK0073-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00568
- InterPro:   IPR003473
- TIGRFAMs:   TIGR00550

Pfam domain/function: PF02445 NadA

EC number: =2.5.1.72

Molecular weight: Translated: 32530; Mature: 32530

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 25-25 BINDING 96-96 BINDING 113-113 BINDING 184-184 BINDING 201-201

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METFVLAHNYQRPDVQLMADSVGDSLELALEAREIDADRIVMCGVDFMAEVVKALNPDRE
CCEEEEECCCCCCCHHHHHHHCCCCEEEEEEHHHCCCCEEEEECHHHHHHHHHHCCCCCE
VVVPDHRAACGMAMRLRAKELREFRREHPDAAVVVYVNTSAEVKAEADVMCTSANAVEVV
EEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEECCCEEEECCHHHHHH
SSLPEDKIIFVPDGNLAAWVQKHVPDKEVIPFPEHGCCPVHHSLSPSDLRELCSQHPDAA
HHCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCE
VVVHPECPLEVCAMADFVGSTSQIRQYCEKEDASKIVMGTEEGLAFRIRRETGTEVIVPG
EEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCEEEECC
HMVCPDMKINTGEKVERVLEARHVPEPLRVELDPDLISQVEEVVEEMFRLTR
CEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
METFVLAHNYQRPDVQLMADSVGDSLELALEAREIDADRIVMCGVDFMAEVVKALNPDRE
CCEEEEECCCCCCCHHHHHHHCCCCEEEEEEHHHCCCCEEEEECHHHHHHHHHHCCCCCE
VVVPDHRAACGMAMRLRAKELREFRREHPDAAVVVYVNTSAEVKAEADVMCTSANAVEVV
EEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEECCCEEEECCHHHHHH
SSLPEDKIIFVPDGNLAAWVQKHVPDKEVIPFPEHGCCPVHHSLSPSDLRELCSQHPDAA
HHCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCE
VVVHPECPLEVCAMADFVGSTSQIRQYCEKEDASKIVMGTEEGLAFRIRRETGTEVIVPG
EEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCEEEECC
HMVCPDMKINTGEKVERVLEARHVPEPLRVELDPDLISQVEEVVEEMFRLTR
CEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014