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The map label for this gene is AmtB [C]

Identifier: 20093493

GI number: 20093493

Start: 51991

End: 52797

Strand: Direct

Name: AmtB [C]

Synonym: MK0053

Alternate gene names: 20093493

Gene position: 51991-52797 (Clockwise)

Preceding gene: 20093492

Following gene: 20093494

Centisome position: 3.07

GC content: 61.21

Gene sequence:

>1294_bases
ATGGCGGCCGAAAGCATAGCTAAGGCCGTGCACGATGTGCTAGCGACGTGGGGTGCCCAAGGACCGGGTGCGATCTTCTT
CCTAGTATGGGCAGCCGTGTTAGTCGTCGGAATGCAGTTAGGTTTCATGTTCCTAGAAGCAGGCCAGGTACGGACGAAGA
ACGTCGTCAGCGTCCTGATGAAGAACCTGCTAGACTTGTCGCTCGGAGGTATTGTCTTCATCTTCTTCGGATTCGCACTG
GCCTTCCCGGACTACGCGCAGAGCATCGGCAAGTGGATTGCCACCATGTTCACGGCGAACCCGTGGAGCGCCGATCCGAT
GAAGGCCGCCGATCCCTACAACCTAGCGTACTGCTTCTTCCAGTTCGCGTTCTGCGCCACGGCGGCGACCATCGTCTCGG
GAGCCGTCGCCGAGCGGATCAACTTCAAGGCGTGCCTGGTGATGACGGTGCTAATCACGGGGCTACTGTACCCGATCTTC
GTGCTGTGGACGTGGGGCGGCGGCTGGATGGGCGGCGACACCGGCCTGTTCGCCAAGGTCTTCGGACAGCCGTACCACGA
CTTCGCCGGATCGACCGTGGTGCACGCGATCGGAGGTTTCCTGGCGATGGCCGCCGCCTACCTGCTGGGTCCGAGGATCG
GGAGGTTCAAGAACGGCAAGCCTGTGCCGATCCCGGGGCACAACATCCCGCAGGCGTTCCTGGGAGCGCTTTTCCTGGCG
ATTACCTGGTACGGTTTCAACGTAGGTAGCTCGGCGGTGCTCTACGACCCGACGAACGAGTCGTGGATCAGCTCGTTAGT
GGCCACTCGCGATGTGTGGTGGAGCCGCGGCGGCCGCCTTCACGACGAGGTTCGACCCACTCTGGGCAGCCAACGGTCTG
ATCGCGGGACTGGTCGCGATCTGCGCCGGATGTGACATCATGAGTCCGTTTGGAGCCCTGCTCACGGGTATCGTGGCGGG
ACTGATCATCAAGCCGGCGTTCCGACTGCTGGAGAAGCTGGAGATCGACGACGTGGTCGCCGCGTGTCCGGTACACGGAT
TCGCGGGCGTTTGGGGAGCGATCGCGGCCGGAATCTTCGGAGCTGAAGCTCTAGGCGGCGCCGGTGGTGTGAGCCTAGCC
GCGCAGATCGTCGGTGCTCTCGTGTGCATAGCCTGGGCGCTGGGCTCAGGATTTGCGACCTTCTACGTGATCGACAAGGT
GATCGGGCTGAGAGCGACGGAAGAGGAGGAGAAAGGTCTGGACGAGACCGAGTTCGGCGTCAGCGCCTACCCGTACATGG
AGACCAGGGATTGA

Upstream 100 bases:

>100_bases
AGTGACCGAAGACGCAGGTGCTCACCTGATATTAAAAGGGGAGCCGAAAGGCGGCAGGAGTTTGCCGGAAAGCCTTGCCG
GATAAAAGTGGGGGAAATCA

Downstream 100 bases:

>100_bases
ACGAGCTGGGGGTGAACCCCGATGTACATGATCGAAGCCGTCATCAGGCCGGAGAAGTTGGACGATGTGAAAGAGGCGCT
GGACGAGGCCGGCTATCCTG

Product: ammonia permease

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>431_residues
MAAESIAKAVHDVLATWGAQGPGAIFFLVWAAVLVVGMQLGFMFLEAGQVRTKNVVSVLMKNLLDLSLGGIVFIFFGFAL
AFPDYAQSIGKWIATMFTANPWSADPMKAADPYNLAYCFFQFAFCATAATIVSGAVAERINFKACLVMTVLITGLLYPIF
VLWTWGGGWMGGDTGLFAKVFGQPYHDFAGSTVVHAIGGFLAMAAAYLLGPRIGRFKNGKPVPIPGHNIPQAFLGALFLA
ITWYGFNVGSSAVLYDPTNESWISSLVATLAMCGGAAAAAFTTRFDPLWAANGLIAGLVAICAGCDIMSPFGALLTGIVA
GLIIKPAFRLLEKLEIDDVVAACPVHGFAGVWGAIAAGIFGAEALGGAGGVSLAAQIVGALVCIAWALGSGFATFYVIDK
VIGLRATEEEEKGLDETEFGVSAYPYMETRD

Sequences:

>Translated_269_residues
MAAESIAKAVHDVLATWGAQGPGAIFFLVWAAVLVVGMQLGFMFLEAGQVRTKNVVSVLMKNLLDLSLGGIVFIFFGFAL
AFPDYAQSIGKWIATMFTANPWSADPMKAADPYNLAYCFFQFAFCATAATIVSGAVAERINFKACLVMTVLITGLLYPIF
VLWTWGGGWMGGDTGLFAKVFGQPYHDFAGSTVVHAIGGFLAMAAAYLLGPRIGRFKNGKPVPIPGHNIPQAFLGALFLA
ITWYGFNVGSSAVLYDPTNESWISSLVAT
>Mature_268_residues
AAESIAKAVHDVLATWGAQGPGAIFFLVWAAVLVVGMQLGFMFLEAGQVRTKNVVSVLMKNLLDLSLGGIVFIFFGFALA
FPDYAQSIGKWIATMFTANPWSADPMKAADPYNLAYCFFQFAFCATAATIVSGAVAERINFKACLVMTVLITGLLYPIFV
LWTWGGGWMGGDTGLFAKVFGQPYHDFAGSTVVHAIGGFLAMAAAYLLGPRIGRFKNGKPVPIPGHNIPQAFLGALFLAI
TWYGFNVGSSAVLYDPTNESWISSLVAT

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=245, Percent_Identity=30.6122448979592, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17550182, Length=239, Percent_Identity=41.4225941422594, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17550180, Length=234, Percent_Identity=38.034188034188, Blast_Score=137, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI133931100, Length=241, Percent_Identity=30.2904564315353, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI32564042, Length=237, Percent_Identity=28.6919831223629, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI161078314, Length=221, Percent_Identity=37.5565610859729, Blast_Score=104, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 28821; Mature: 28690

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCCEEEECCCCHHHHHHHHCC
>Mature Secondary Structure 
AAESIAKAVHDVLATWGAQGPGAIFFLVWAAVLVVGMQLGFMFLEAGQVRTKNVVSVLM
CHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
KNLLDLSLGGIVFIFFGFALAFPDYAQSIGKWIATMFTANPWSADPMKAADPYNLAYCFF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
QFAFCATAATIVSGAVAERINFKACLVMTVLITGLLYPIFVLWTWGGGWMGGDTGLFAKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
FGQPYHDFAGSTVVHAIGGFLAMAAAYLLGPRIGRFKNGKPVPIPGHNIPQAFLGALFLA
HCCCHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ITWYGFNVGSSAVLYDPTNESWISSLVAT
HHHHHHCCCCCEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]