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The map label for this gene is bioB [C]

Identifier: 20093456

GI number: 20093456

Start: 15236

End: 16306

Strand: Direct

Name: bioB [C]

Synonym: MK0016

Alternate gene names: 20093456

Gene position: 15236-16306 (Clockwise)

Preceding gene: 20093455

Following gene: 20093457

Centisome position: 0.9

GC content: 60.6

Gene sequence:

>1071_bases
GTGCGTTTCAAAGACGCGCTGCGCGAAGTCAGGACGGATCGTAAGTTGGCCGATACCGAGGCCATCGTTCGCTTACTCTC
CGCCAAATCGGTTAGGGTACACGACCTGTTCCGAGCCGCCCTGAGCGAGAAACTCCACCATCGCGGAGAGCTGGTGAAGC
TCACGTCAACGATACACGTAACCAACGAGTGCCGCATCCGGCCGAGATGTGCGTACTGCGGCTTCGCCGCGGGCGCGTCC
CCGGAGGGTTACTTCGAGGGATTCACACGGAGCTACGAGGAGATCGCAGAGGCCGCGAAAGCCATCGAGGAATCGGGAAT
CCCACGCGTCAGCTGCTCGGGAGCTTATCGCGGAGACGGGGGTAAGCTGGCCGTGACCGCCGCTAGGGCGGTGAAGGAGA
ACACCGACCTGGAGCTCCTCATCAACTTCGGCCACGACCTGTCCGAAGAGACCATAGCGGAGCTCGCGAGGCTAGACGTG
GAAACCATTTGCTGTAATTTGGAAACCACTAATAGGGAGCTGTTCGAAAGGTTGAAACCCGGAGACAGCTTCGAGGAGCG
CGTCCGTGTGTGCGAGACCGTATGTCGATACGGCATTGACCTGTCAAGCGGCCTCCTAGTGGGAATCGGTGAGGACTACA
GGGACCGAGCCGAGCATCTGAAGTTCCTCGCGAGGTTCGAAACCCTCGCGGAAATACCCATTATGGGGTTCAACCCGTAT
CCCGGTACACTGATGGAACACGTGCCCCGTTGTCCGCTGTTGGAGCAGGCGAAGGTCATGGCCGTAGCCAGACTGATGTA
CCCCGACTTAATGATCACCGCCCCCACACCGACCGTGGGACCGGAGGAAGTCGAGGTGGCACTGATGGCGGGTGCCGACA
ACCTGGCGACCGTGATACCGGACAACCATCCGCACGAGGTTAAGGGTGTCGGAAACCCGCGGACCGGCAACCTGGATAGG
GTCGTGGAGCTCATCGAGGGGTTCGGGCTCAAGCCCGAGCTCAGGGGTGACCGAGCTTGCTCGACCTCCTGCACGAAGCT
TGTGAATCGCTCGACGCAGCGCTCGAGCTGA

Upstream 100 bases:

>100_bases
GAGCTGCCCGGGTTGAAGGAGCTCGCAGAGGAACTCTCGAAACGCTTGCGGGTGCGGGTAGAGCTGAGGTCGCCCGAGCA
TCCGGTGGGGGATCATTATC

Downstream 100 bases:

>100_bases
AGCGGCTGGCTTACGAGGGGAAGCTAAGCGTCGATGAGGTGGTTACGGACCTGGGAGAACTCACGGATCGGGAACTCGAA
GCCCTAGGTGACAATCTGCG

Product: biotin synthase

Products: biotin

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MRFKDALREVRTDRKLADTEAIVRLLSAKSVRVHDLFRAALSEKLHHRGELVKLTSTIHVTNECRIRPRCAYCGFAAGAS
PEGYFEGFTRSYEEIAEAAKAIEESGIPRVSCSGAYRGDGGKLAVTAARAVKENTDLELLINFGHDLSEETIAELARLDV
ETICCNLETTNRELFERLKPGDSFEERVRVCETVCRYGIDLSSGLLVGIGEDYRDRAEHLKFLARFETLAEIPIMGFNPY
PGTLMEHVPRCPLLEQAKVMAVARLMYPDLMITAPTPTVGPEEVEVALMAGADNLATVIPDNHPHEVKGVGNPRTGNLDR
VVELIEGFGLKPELRGDRACSTSCTKLVNRSTQRSS

Sequences:

>Translated_356_residues
MRFKDALREVRTDRKLADTEAIVRLLSAKSVRVHDLFRAALSEKLHHRGELVKLTSTIHVTNECRIRPRCAYCGFAAGAS
PEGYFEGFTRSYEEIAEAAKAIEESGIPRVSCSGAYRGDGGKLAVTAARAVKENTDLELLINFGHDLSEETIAELARLDV
ETICCNLETTNRELFERLKPGDSFEERVRVCETVCRYGIDLSSGLLVGIGEDYRDRAEHLKFLARFETLAEIPIMGFNPY
PGTLMEHVPRCPLLEQAKVMAVARLMYPDLMITAPTPTVGPEEVEVALMAGADNLATVIPDNHPHEVKGVGNPRTGNLDR
VVELIEGFGLKPELRGDRACSTSCTKLVNRSTQRSS
>Mature_356_residues
MRFKDALREVRTDRKLADTEAIVRLLSAKSVRVHDLFRAALSEKLHHRGELVKLTSTIHVTNECRIRPRCAYCGFAAGAS
PEGYFEGFTRSYEEIAEAAKAIEESGIPRVSCSGAYRGDGGKLAVTAARAVKENTDLELLINFGHDLSEETIAELARLDV
ETICCNLETTNRELFERLKPGDSFEERVRVCETVCRYGIDLSSGLLVGIGEDYRDRAEHLKFLARFETLAEIPIMGFNPY
PGTLMEHVPRCPLLEQAKVMAVARLMYPDLMITAPTPTVGPEEVEVALMAGADNLATVIPDNHPHEVKGVGNPRTGNLDR
VVELIEGFGLKPELRGDRACSTSCTKLVNRSTQRSS

Specific function: Biotin biosynthesis; last step. [C]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: 2.8.1.6

Molecular weight: Translated: 39362; Mature: 39362

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFKDALREVRTDRKLADTEAIVRLLSAKSVRVHDLFRAALSEKLHHRGELVKLTSTIHV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
TNECRIRPRCAYCGFAAGASPEGYFEGFTRSYEEIAEAAKAIEESGIPRVSCSGAYRGDG
CCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEECCC
GKLAVTAARAVKENTDLELLINFGHDLSEETIAELARLDVETICCNLETTNRELFERLKP
CEEEEEHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCHHHHEECCCCCCHHHHHHCCC
GDSFEERVRVCETVCRYGIDLSSGLLVGIGEDYRDRAEHLKFLARFETLAEIPIMGFNPY
CCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PGTLMEHVPRCPLLEQAKVMAVARLMYPDLMITAPTPTVGPEEVEVALMAGADNLATVIP
CCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHEEEEEEECCCCCEEECC
DNHPHEVKGVGNPRTGNLDRVVELIEGFGLKPELRGDRACSTSCTKLVNRSTQRSS
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRFKDALREVRTDRKLADTEAIVRLLSAKSVRVHDLFRAALSEKLHHRGELVKLTSTIHV
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
TNECRIRPRCAYCGFAAGASPEGYFEGFTRSYEEIAEAAKAIEESGIPRVSCSGAYRGDG
CCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCEECCC
GKLAVTAARAVKENTDLELLINFGHDLSEETIAELARLDVETICCNLETTNRELFERLKP
CEEEEEHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCHHHHEECCCCCCHHHHHHCCC
GDSFEERVRVCETVCRYGIDLSSGLLVGIGEDYRDRAEHLKFLARFETLAEIPIMGFNPY
CCCHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PGTLMEHVPRCPLLEQAKVMAVARLMYPDLMITAPTPTVGPEEVEVALMAGADNLATVIP
CCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCHHHEEEEEEECCCCCEEECC
DNHPHEVKGVGNPRTGNLDRVVELIEGFGLKPELRGDRACSTSCTKLVNRSTQRSS
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.002 {dethiobiotin}} [C]

Substrates: dethiobiotin; sulfur

Specific reaction: dethiobiotin + sulfur = biotin

General reaction: Sulfur atom transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]