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The map label for this gene is Hmd

Identifier: 20093453

GI number: 20093453

Start: 12672

End: 13748

Strand: Direct

Name: Hmd

Synonym: MK0013

Alternate gene names: 20093453

Gene position: 12672-13748 (Clockwise)

Preceding gene: 20093451

Following gene: 20093454

Centisome position: 0.75

GC content: 61.47

Gene sequence:

>1077_bases
ATGGTCGAGATCAACAAAGTCGCGATTTTAGGCGCCGGATGTTGGAGAACCCACGCCGCGACGGGCATCACGACGTTCAA
GCGTGCGTGTGAGGTGGCCGATGAGACCGGCATCAAAGAGGCCGCTCTCACGCACTCCAGCGTGACGTACGCCGTCGAGC
TGAAGCACCTGGCGGGCGTCGACGAGGTCGTCCTCTCCGACCCCGTGTTCGATGCCGATGGCTTCACGGTCGTGGACATC
GAAGAGGACTGCGACGTGGATCTGGACGAGTTCATCAAGGCGCACCTCGAGGGTAACCCCGAGGACGTGATGCCGAAGCT
ACGGGACTACGTGAACGACATCGCCGATGACGTCCCGAAGCCGCCCAAGGGAGCCATCCACTTCCTGAGCCCCGAGGAGA
TGGAGGACAAGCTCGACATCGTCGTGACCACAGACGACGCGGAGGCCGTCGAGGACGCGGACATGATCATCTCCTGGTTG
CCGAAAGGCGGAGTTCAGCCTGATATTTTCAAGAAGATCATCGACGACATCCCGGAAGGCTGCATCGTAGCCAACACCTG
TACGATCCCGACGCGACAGTTCAAGGAGATGTTCGAAGACATGGGCCGCGATGATCTGCAGGTAACCTCCTACCACCCGG
CGACCGTGCCGGAGCACAAGGGTCAGGTGTTCGTGGCAGAAGGTTACGCGGACGAGGAAGTTGTCGAGGCCGTCTACGAG
CTGGGTGAGAAGGCCCGCGGACTGGCGTTCAAGGTCCCGGGCTACCTGCTGGGCCCGGTGTGCGACATGGCCAGCGCCGT
CACGGCGATCGTGTACGCCGGCCTGCTGACGTTCCGAGATGCGTGTACCGACATCCTAGGAGCTCCCGTGGACTTCACCC
AGAACATGGCCGTAGAAGCACTGCAGATGATGGCGAAGTTCATGGAGGAAGAGGGTCTAGACAAGCTAGAGGAGGCCCTG
GACCCGGCGGCGCTCACGAACACGGCCGACTCGATGAACTTCGGCCCGCTGGCGGACACGGAGATCCTGCCGAAGGCCCT
AGAGGTCCTGGAGAAGTACTCGAAGAAGGCGGAGTAA

Upstream 100 bases:

>100_bases
TTTAACCGTTATCCCGTTGGAGCAGTCGGTGCGCTAATTAATCTCACCACCCGGAGGGATGACGCATTGGTAAAGATACC
CTAACACGGGGTGCCCACGC

Downstream 100 bases:

>100_bases
GCCCCTTTACTTTCCCCCACAGTCTCTTTCCAGGGGAGCTCGTTGAAGGAACCGGAACCGGGAATAACCGATCTCACGGA
GACCGTCCGGTCACGTCCGT

Product: H(2)-dependent methylenetetrahydromethanopterin dehydrogenase

Products: NA

Alternate protein names: H(2)-dependent methylene-H(4)MPT dehydrogenase; H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase; N(5),N(10)-methenyltetrahydromethanopterin hydrogenase

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MVEINKVAILGAGCWRTHAATGITTFKRACEVADETGIKEAALTHSSVTYAVELKHLAGVDEVVLSDPVFDADGFTVVDI
EEDCDVDLDEFIKAHLEGNPEDVMPKLRDYVNDIADDVPKPPKGAIHFLSPEEMEDKLDIVVTTDDAEAVEDADMIISWL
PKGGVQPDIFKKIIDDIPEGCIVANTCTIPTRQFKEMFEDMGRDDLQVTSYHPATVPEHKGQVFVAEGYADEEVVEAVYE
LGEKARGLAFKVPGYLLGPVCDMASAVTAIVYAGLLTFRDACTDILGAPVDFTQNMAVEALQMMAKFMEEEGLDKLEEAL
DPAALTNTADSMNFGPLADTEILPKALEVLEKYSKKAE

Sequences:

>Translated_358_residues
MVEINKVAILGAGCWRTHAATGITTFKRACEVADETGIKEAALTHSSVTYAVELKHLAGVDEVVLSDPVFDADGFTVVDI
EEDCDVDLDEFIKAHLEGNPEDVMPKLRDYVNDIADDVPKPPKGAIHFLSPEEMEDKLDIVVTTDDAEAVEDADMIISWL
PKGGVQPDIFKKIIDDIPEGCIVANTCTIPTRQFKEMFEDMGRDDLQVTSYHPATVPEHKGQVFVAEGYADEEVVEAVYE
LGEKARGLAFKVPGYLLGPVCDMASAVTAIVYAGLLTFRDACTDILGAPVDFTQNMAVEALQMMAKFMEEEGLDKLEEAL
DPAALTNTADSMNFGPLADTEILPKALEVLEKYSKKAE
>Mature_358_residues
MVEINKVAILGAGCWRTHAATGITTFKRACEVADETGIKEAALTHSSVTYAVELKHLAGVDEVVLSDPVFDADGFTVVDI
EEDCDVDLDEFIKAHLEGNPEDVMPKLRDYVNDIADDVPKPPKGAIHFLSPEEMEDKLDIVVTTDDAEAVEDADMIISWL
PKGGVQPDIFKKIIDDIPEGCIVANTCTIPTRQFKEMFEDMGRDDLQVTSYHPATVPEHKGQVFVAEGYADEEVVEAVYE
LGEKARGLAFKVPGYLLGPVCDMASAVTAIVYAGLLTFRDACTDILGAPVDFTQNMAVEALQMMAKFMEEEGLDKLEEAL
DPAALTNTADSMNFGPLADTEILPKALEVLEKYSKKAE

Specific function: Catalyzes the reversible reduction of methenyl- H(4)MPT(+) to methylene-H(4)MPT

COG id: COG4074

COG function: function code C; H2-forming N5,N10-methylenetetrahydromethanopterin dehydrogenase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMD family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HMD_METKA (Q02394)

Other databases:

- EMBL:   X60719
- EMBL:   Y10251
- EMBL:   AE009439
- PIR:   S30603
- RefSeq:   NP_613300.1
- ProteinModelPortal:   Q02394
- SMR:   Q02394
- GeneID:   1477315
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0013
- NMPDR:   fig|190192.1.peg.13
- HOGENOM:   HBG540926
- OMA:   THACTIP
- ProtClustDB:   PRK00961
- BioCyc:   MKAN190192:MK0013-MONOMER
- BRENDA:   1.12.98.2
- GO:   GO:0005488
- HAMAP:   MF_01090
- InterPro:   IPR008927
- InterPro:   IPR004889
- InterPro:   IPR010062
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01723

Pfam domain/function: PF03201 HMD; SSF48179 6DGDH_C_like

EC number: =1.12.98.2

Molecular weight: Translated: 39039; Mature: 39039

Theoretical pI: Translated: 4.01; Mature: 4.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEINKVAILGAGCWRTHAATGITTFKRACEVADETGIKEAALTHSSVTYAVELKHLAGV
CCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEEEHHHHCCC
DEVVLSDPVFDADGFTVVDIEEDCDVDLDEFIKAHLEGNPEDVMPKLRDYVNDIADDVPK
HHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
PPKGAIHFLSPEEMEDKLDIVVTTDDAEAVEDADMIISWLPKGGVQPDIFKKIIDDIPEG
CCCCCEEECCHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
CIVANTCTIPTRQFKEMFEDMGRDDLQVTSYHPATVPEHKGQVFVAEGYADEEVVEAVYE
CEEEEECCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHH
LGEKARGLAFKVPGYLLGPVCDMASAVTAIVYAGLLTFRDACTDILGAPVDFTQNMAVEA
HHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LQMMAKFMEEEGLDKLEEALDPAALTNTADSMNFGPLADTEILPKALEVLEKYSKKAE
HHHHHHHHHHCCHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVEINKVAILGAGCWRTHAATGITTFKRACEVADETGIKEAALTHSSVTYAVELKHLAGV
CCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEEEHHHHCCC
DEVVLSDPVFDADGFTVVDIEEDCDVDLDEFIKAHLEGNPEDVMPKLRDYVNDIADDVPK
HHHHHCCCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
PPKGAIHFLSPEEMEDKLDIVVTTDDAEAVEDADMIISWLPKGGVQPDIFKKIIDDIPEG
CCCCCEEECCHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
CIVANTCTIPTRQFKEMFEDMGRDDLQVTSYHPATVPEHKGQVFVAEGYADEEVVEAVYE
CEEEEECCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCEEEECCCCCHHHHHHHHH
LGEKARGLAFKVPGYLLGPVCDMASAVTAIVYAGLLTFRDACTDILGAPVDFTQNMAVEA
HHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LQMMAKFMEEEGLDKLEEALDPAALTNTADSMNFGPLADTEILPKALEVLEKYSKKAE
HHHHHHHHHHCCHHHHHHHCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1521540; 9151968; 11930014; 1772345