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The map label for this gene is proB [H]

Identifier: 19746586

GI number: 19746586

Start: 1399267

End: 1400088

Strand: Reverse

Name: proB [H]

Synonym: spyM18_1681

Alternate gene names: 19746586

Gene position: 1400088-1399267 (Counterclockwise)

Preceding gene: 19746587

Following gene: 19746585

Centisome position: 73.88

GC content: 39.66

Gene sequence:

>822_bases
ATGATGAAACGACAATTTGAAGATGTAACACGTATCGTAATTAAAATTGGCACTAGTTCATTAGTGCTACCAACAGGTAA
AATCAATTTAGAAAAAATCGACCAACTGGCTTTTGTGATTTCAAGTTTGATGAATAAGGGCAAGGAAGTTATTTTAGTAT
CATCGGGAGCGATGGGATTTGGACTAGATATTTTGAAAATGGAAAAACGTCCGACCAATCTTGCTAAACAACAGGCAGTT
TCCAGTGTTGGTCAGGTGGCCATGATGAGTCTTTATTCACAAATTTTTGCACACTACCAAACCAACGTGTCACAAATTTT
ATTGACGAGAGATGTGGTGGTTTTCCCAGAAAGTCTAGCCAATGTTACCAATGCTTTTGAAAGTCTGATTAGTTTGGGCA
TTGTGCCGATTGTTAATGAAAACGATGCGGTTAGTGTGGATGAAATGGATCATGCGACTAAATTTGGAGATAATGACCGT
TTATCTGCTGTCGTTGCTGGTATTACAAAAGCTGATTTATTAATCATGTTGTCTGATATTGATGGCCTTTTTGATAAAAA
CCCTACTATTTATGAGGATGCGCAGTTACGCAGTCATGTTGCAGTCGTTACGCAAGAAATCATTGCGTCAGCAGGTGGTG
CGGGCAGTAAATTTGGCACAGGTGGGATGCTTAGCAAAATTCAGTCTGCTCAGATGGTATTTGAAAACAAAGGACAGATG
GTCTTGATGAATGGAGCTAATCCCAGAGATATTTTAAGAGTGCTTGAAGGGCAACCACTAGGCACCTGGTTCAAGCAGAT
AGAAGAGGTAACACATGACTGA

Upstream 100 bases:

>100_bases
TCAAAAAACCTTCTGGAAAAGCTTAGAGAAGCTTTAACGAAGAGAGATCATTATTCGGAAAAACAGTCCTCTGGGGCTGT
TTTTATGCTATAATTTGGGT

Downstream 100 bases:

>100_bases
TATGAGAAGGTTAGGGCAAAGGGCAAAACAAGCTAGTCTTTTAATAGCACCTTTGTCGACGCAAATTAAAAATCGCTTTT
TAAGCACCTTAGCAAAGGCA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLMNKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAV
SSVGQVAMMSLYSQIFAHYQTNVSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVAVVTQEIIASAGGAGSKFGTGGMLSKIQSAQMVFENKGQM
VLMNGANPRDILRVLEGQPLGTWFKQIEEVTHD

Sequences:

>Translated_273_residues
MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLMNKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAV
SSVGQVAMMSLYSQIFAHYQTNVSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVAVVTQEIIASAGGAGSKFGTGGMLSKIQSAQMVFENKGQM
VLMNGANPRDILRVLEGQPLGTWFKQIEEVTHD
>Mature_273_residues
MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLMNKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAV
SSVGQVAMMSLYSQIFAHYQTNVSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVAVVTQEIIASAGGAGSKFGTGGMLSKIQSAQMVFENKGQM
VLMNGANPRDILRVLEGQPLGTWFKQIEEVTHD

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=297, Percent_Identity=29.6296296296296, Blast_Score=119, Evalue=2e-27,
Organism=Homo sapiens, GI21361368, Length=299, Percent_Identity=29.7658862876254, Blast_Score=118, Evalue=5e-27,
Organism=Escherichia coli, GI1786437, Length=259, Percent_Identity=33.976833976834, Blast_Score=144, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17569967, Length=275, Percent_Identity=31.2727272727273, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17569969, Length=275, Percent_Identity=31.2727272727273, Blast_Score=127, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=250, Percent_Identity=36, Blast_Score=158, Evalue=8e-40,
Organism=Saccharomyces cerevisiae, GI6321822, Length=248, Percent_Identity=33.8709677419355, Blast_Score=152, Evalue=4e-38,
Organism=Drosophila melanogaster, GI21357643, Length=295, Percent_Identity=31.864406779661, Blast_Score=134, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 29661; Mature: 29661

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLMNKGKEVILVSSGAMGF
CCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAHYQTNVSQILLTRDVVVFPESLA
CHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCHHHH
NVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDRLSAVVAGITKADLLIMLSDI
HHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEHHH
DGLFDKNPTIYEDAQLRSHVAVVTQEIIASAGGAGSKFGTGGMLSKIQSAQMVFENKGQM
HHHHCCCCCEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCE
VLMNGANPRDILRVLEGQPLGTWFKQIEEVTHD
EEECCCCHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLMNKGKEVILVSSGAMGF
CCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAHYQTNVSQILLTRDVVVFPESLA
CHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCHHHH
NVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDRLSAVVAGITKADLLIMLSDI
HHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEHHH
DGLFDKNPTIYEDAQLRSHVAVVTQEIIASAGGAGSKFGTGGMLSKIQSAQMVFENKGQM
HHHHCCCCCEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCE
VLMNGANPRDILRVLEGQPLGTWFKQIEEVTHD
EEECCCCHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA