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The map label for this gene is rlmN [H]

Identifier: 19746482

GI number: 19746482

Start: 1279712

End: 1280791

Strand: Reverse

Name: rlmN [H]

Synonym: spyM18_1550

Alternate gene names: 19746482

Gene position: 1280791-1279712 (Counterclockwise)

Preceding gene: 19746483

Following gene: 19746480

Centisome position: 67.59

GC content: 37.59

Gene sequence:

>1080_bases
ATGAAACCATCGATTTATAGCTTAACACGCGATGAACTGATCGCGTGGGCAGTTGAACGCGGCCAAAAACAGTTTAGAGC
TACTCAAATTTGGGATTGGCTCTATAAAAAAAGAGTTCAATCTTTTGAGGAGATGACGAATATTTCTAAAGATTTTGTTC
CTATTTTAAATGATAGTTTCTGTGTCAATCCTTTAAAACAGCGAGTTGTACAAGAATCAGCTGATGGTACAGTAAAATAT
TTATTTGAACTGCCCGATGGTATGTTAATTGAAACAGTTTTAATGCGTCAACATTATGGTCATTCTGTTTGTGTGACGAC
TCAAGTTGGCTGTAACATTGGCTGTACTTTTTGTGCTAGTGGATTGATCAAAAAGCAACGTGATCTAAACAGTGGGGAAA
TTACAGCTCAAATCATGTTAGTCCAAAAATATTTTGACGATAGAAAGCAAGGTGAGCGGGTTAGCCATGTTGTCGTAATG
GGCATTGGTGAACCTTTTGATAATTACAAAAATGTCATGTGCTTTCTTCGCGTCATCAACGATGATAATGGCTTAGCAAT
TGGTGCCCGTCATATTACAGTCTCTACCTCAGGGTTAGCTCACAAGATTCGGGATTTTGCAAACGAGGGAGTACAGGTTA
ATTTAGCAGTCTCGTTGCATGCACCAAATAATGATTTACGTTCAAGTATTATGCGAGTAAATCGCTCTTTTCCGCTGGAA
AAGCTCTTTTCAGCTATAGAATACTATATTGAAAAAACGAATCGTCGTGTCACTTTTGAATACATTATGCTAAATGAAGT
TAACGATAGCATTAAGCAAGCTCAAGAACTAGCAGACTTAACTAAAACTATTCGTAAGTTATCTTATGTTAACTTGATTC
CGTATAATCCTGTTTCAGAACATGATCAGTATAGTAGAAGTCCTAAAGAGCGAGTTCTAGCGTTTTATGATGTGCTAAAG
AAAAATGGAGTTAACTGTGTGGTGCGTCAAGAGCACGGAACAGATATTGATGCAGCCTGTGGCCAGTTACGTTCTAAAAC
GATGAAAAAAGATCGCGAAAAGGTTACAGCGACAAAATAA

Upstream 100 bases:

>100_bases
AAGTAATACTAAGCATAAAGAAAAGTCAAAACGTAATCAGACTAGTCAACTTAATACCAAAATTAGTCATTTTATCATCA
GAAAGAAAGATAAATAATCC

Downstream 100 bases:

>100_bases
AGTCCAAAAAATATCTTTGATTGACAATGCTTGATGACAAAATATTCCTAGTCACTAAAATGACTAGGTTTTTTATCTGG
CCTAAAAATGCTCTAGAAGC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEEMTNISKDFVPILNDSFCVNPLKQRVVQESADGTVKY
LFELPDGMLIETVLMRQHYGHSVCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVSLHAPNNDLRSSIMRVNRSFPLE
KLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQELADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLK
KNGVNCVVRQEHGTDIDAACGQLRSKTMKKDREKVTATK

Sequences:

>Translated_359_residues
MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEEMTNISKDFVPILNDSFCVNPLKQRVVQESADGTVKY
LFELPDGMLIETVLMRQHYGHSVCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVSLHAPNNDLRSSIMRVNRSFPLE
KLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQELADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLK
KNGVNCVVRQEHGTDIDAACGQLRSKTMKKDREKVTATK
>Mature_359_residues
MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEEMTNISKDFVPILNDSFCVNPLKQRVVQESADGTVKY
LFELPDGMLIETVLMRQHYGHSVCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVSLHAPNNDLRSSIMRVNRSFPLE
KLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQELADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLK
KNGVNCVVRQEHGTDIDAACGQLRSKTMKKDREKVTATK

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=363, Percent_Identity=35.8126721763085, Blast_Score=217, Evalue=8e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41064; Mature: 41064

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEEMTNISKDFVPILNDSF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
CVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHSVCVTTQVGCNIGCTFCAS
CCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
GLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVMGIGEPFDNYKNVMCFLRVIN
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHEEE
DDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVSLHAPNNDLRSSIMRVNRSFPLE
CCCCEEEEEEEEEEECHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCHH
KLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQELADLTKTIRKLSYVNLIPYNPVSE
HHHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
HDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGTDIDAACGQLRSKTMKKDREKVTATK
CHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEEMTNISKDFVPILNDSF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCC
CVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHSVCVTTQVGCNIGCTFCAS
CCCHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
GLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVMGIGEPFDNYKNVMCFLRVIN
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHEEE
DDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVSLHAPNNDLRSSIMRVNRSFPLE
CCCCEEEEEEEEEEECHHHHHHHHHHHCCCEEEEEEEEEECCCHHHHHHHHHHHCCCCHH
KLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQELADLTKTIRKLSYVNLIPYNPVSE
HHHHHHHHHHHHCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
HDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGTDIDAACGQLRSKTMKKDREKVTATK
CHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]