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The map label for this gene is ygcA [C]

Identifier: 19746322

GI number: 19746322

Start: 1131620

End: 1133008

Strand: Reverse

Name: ygcA [C]

Synonym: spyM18_1360

Alternate gene names: 19746322

Gene position: 1133008-1131620 (Counterclockwise)

Preceding gene: 19746323

Following gene: 19746321

Centisome position: 59.79

GC content: 41.18

Gene sequence:

>1389_bases
ATGGTTAGCCCTAGAAAAGGAAAAAGAATAAGAATGTTGAAAAAAAATGATATTATCCAAGTTGCTATTTCAGATTTAAG
CCATGAGGGAGCAGGTGTCGCTAAACACGATGGTTTTGTTTTCTTTGTTGATAATGCCTTGCCAGAAGAAGTGATTGACA
TGCGTGTCTTAAAGGTCAATAAAAACAGCGGTTTTGGCAAAGTAGAAGCCTATCATTACCTGTCGCCAGCTAGAAATGCA
GATGTCAATTTAACTTATTTGCGAACAGGCATTGCAGACCTTGGACATCTAACTTATGAAGACCAACTGACTTTCAAAAA
GAAACAAGTTCAGGACAGCCTCTATAAAATTGCTGGAATCAGTGACGTGACAGTCGAAAGCACGATTGGGATGACAGAAC
CTTTAGCTTACCGAAATAAGGCTCAAGTTCCTGTTAGACGTGTTAATGGTCAGCTAGAAACAGGCTTTTTCCGCAAGCAT
TCTCATGACTTGATTCCTATTTCGGATTATTATATTCAGGACAAAGAGATTGATCGTCTCATTAATTTTACCCGTGATTT
ATTACGCCGCTTTGATATTAAACCGTATGACGAAACAGAACAAACAGGTCTCTTGAGAAATATTGTTGTTCGCCGAGGCC
ATTACTCTGGAGAAATGATGGTGGTCTTAGTGACTACACGCCCAAAAGTTTTCCGTGTTGACCAAGTCATTGAAAAAATC
GTTGAAGCTTTTCCAGCAGTGGTGTCTATTATTCAAAATATTAATGATGAGAACACCAATGCAATCTTTGGTAAAGATTT
CAAAACGCTTTACGGCAAGGATACCATTACTGATAGCATGCTTGGAAACAACTATGCCATTTCTGCCCAGTCTTTCTACC
AAGTGAATACGGTTATGGCAGAAAAACTCTATCAAACAGCTATAGCCTTTTCAGACCTTTCTAAAGATGACATTGTGATT
GACGCCTACTCAGGTATCGGAACCATTGGCCTATCATTTGCTAAAACAGTTAAAGCTGTTTATGGTGTTGAAGTGATTGA
AGCAGCTGTTAGAGATGCTCAACAGAATGCCGCTCTTAATGGCATCACCAATGCCTACTTTGTCGCAGATACCGCAGAAC
ATGCCATGGCAACATGGGCGAAAGATGGCATTAAACCAAGTGTTATTCTGGTAGACCCGCCACGAAAAGGCTTAACAGAA
AGTTTCATCCAAGCCAGCGTGGCAATGGGACCACAAAAAATTACCTACGTTTCCTGCAACCCAGCCACCATGGCGCGTGA
CATCAAGCGTTATCAAGAGTTGGGTTACAAACTGGCCAAAGTACAGCCCGTAGATTTGTTTCCGCAAACGCATCACGTCG
AGTGCGTAGTGTTGCTAATCAAGGAGTAA

Upstream 100 bases:

>100_bases
CATAGATTTTGATAAGTATCAGAAAACGTTAGCCAAATTAGGTCTGCGCTAAGATATGGTATAATGGAGAGTAACTAAAG
TTGCTCTTCTTTTTTGAGCA

Downstream 100 bases:

>100_bases
GGCAGCTGCCTCCACATGACAAAAATAAGGAGAAACGAATGCCTATTACCAAACACGAGATTCCCCTTTTAGAATGTGAT
AGTAATCCATCAGCCGTCAT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MVSPRKGKRIRMLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMRVLKVNKNSGFGKVEAYHYLSPARNA
DVNLTYLRTGIADLGHLTYEDQLTFKKKQVQDSLYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKH
SHDLIPISDYYIQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGEMMVVLVTTRPKVFRVDQVIEKI
VEAFPAVVSIIQNINDENTNAIFGKDFKTLYGKDTITDSMLGNNYAISAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVI
DAYSGIGTIGLSFAKTVKAVYGVEVIEAAVRDAQQNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVDPPRKGLTE
SFIQASVAMGPQKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQTHHVECVVLLIKE

Sequences:

>Translated_462_residues
MVSPRKGKRIRMLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMRVLKVNKNSGFGKVEAYHYLSPARNA
DVNLTYLRTGIADLGHLTYEDQLTFKKKQVQDSLYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKH
SHDLIPISDYYIQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGEMMVVLVTTRPKVFRVDQVIEKI
VEAFPAVVSIIQNINDENTNAIFGKDFKTLYGKDTITDSMLGNNYAISAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVI
DAYSGIGTIGLSFAKTVKAVYGVEVIEAAVRDAQQNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVDPPRKGLTE
SFIQASVAMGPQKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQTHHVECVVLLIKE
>Mature_462_residues
MVSPRKGKRIRMLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMRVLKVNKNSGFGKVEAYHYLSPARNA
DVNLTYLRTGIADLGHLTYEDQLTFKKKQVQDSLYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKH
SHDLIPISDYYIQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGEMMVVLVTTRPKVFRVDQVIEKI
VEAFPAVVSIIQNINDENTNAIFGKDFKTLYGKDTITDSMLGNNYAISAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVI
DAYSGIGTIGLSFAKTVKAVYGVEVIEAAVRDAQQNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVDPPRKGLTE
SFIQASVAMGPQKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQTHHVECVVLLIKE

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI269784680, Length=425, Percent_Identity=27.2941176470588, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI269784676, Length=425, Percent_Identity=27.2941176470588, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI20149688, Length=425, Percent_Identity=27.2941176470588, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI34222389, Length=296, Percent_Identity=29.7297297297297, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI51173878, Length=296, Percent_Identity=29.7297297297297, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI269784678, Length=357, Percent_Identity=27.7310924369748, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1789148, Length=464, Percent_Identity=27.1551724137931, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1787083, Length=331, Percent_Identity=27.4924471299094, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1790403, Length=186, Percent_Identity=32.258064516129, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71997438, Length=509, Percent_Identity=23.5756385068762, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322909, Length=443, Percent_Identity=24.1534988713318, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24666579, Length=361, Percent_Identity=29.0858725761773, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 51730; Mature: 51730

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS50926 TRAM ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSPRKGKRIRMLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMRVLKVN
CCCCCCCCEEEEECCCCEEEEEEHHCCCCCCCEEECCCEEEEECCCCCHHHHCEEEEEEE
KNSGFGKVEAYHYLSPARNADVNLTYLRTGIADLGHLTYEDQLTFKKKQVQDSLYKIAGI
CCCCCCEEEEEEEECCCCCCCCEEEEEHHHHHHHCCCEECHHHHHHHHHHHHHHHHHCCC
SDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKHSHDLIPISDYYIQDKEIDRL
CCEEEHHHCCCCCCHHCCCCCCCCHHHHCCCCCCCHHHHCCCCEEECHHCEECHHHHHHH
INFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGEMMVVLVTTRPKVFRVDQVIEKI
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEHHHHHHHH
VEAFPAVVSIIQNINDENTNAIFGKDFKTLYGKDTITDSMLGNNYAISAQSFYQVNTVMA
HHHHHHHHHHHHHCCCCCCCEEECCCHHHHCCCCHHHHHHCCCCEEEEHHHHHHHHHHHH
EKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFAKTVKAVYGVEVIEAAVRDAQQNAALN
HHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GITNAYFVADTAEHAMATWAKDGIKPSVILVDPPRKGLTESFIQASVAMGPQKITYVSCN
CCCCEEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECC
PATMARDIKRYQELGYKLAKVQPVDLFPQTHHVECVVLLIKE
HHHHHHHHHHHHHHCCEEHEEECCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure
MVSPRKGKRIRMLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMRVLKVN
CCCCCCCCEEEEECCCCEEEEEEHHCCCCCCCEEECCCEEEEECCCCCHHHHCEEEEEEE
KNSGFGKVEAYHYLSPARNADVNLTYLRTGIADLGHLTYEDQLTFKKKQVQDSLYKIAGI
CCCCCCEEEEEEEECCCCCCCCEEEEEHHHHHHHCCCEECHHHHHHHHHHHHHHHHHCCC
SDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKHSHDLIPISDYYIQDKEIDRL
CCEEEHHHCCCCCCHHCCCCCCCCHHHHCCCCCCCHHHHCCCCEEECHHCEECHHHHHHH
INFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGEMMVVLVTTRPKVFRVDQVIEKI
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEHHHHHHHH
VEAFPAVVSIIQNINDENTNAIFGKDFKTLYGKDTITDSMLGNNYAISAQSFYQVNTVMA
HHHHHHHHHHHHHCCCCCCCEEECCCHHHHCCCCHHHHHHCCCCEEEEHHHHHHHHHHHH
EKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFAKTVKAVYGVEVIEAAVRDAQQNAALN
HHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GITNAYFVADTAEHAMATWAKDGIKPSVILVDPPRKGLTESFIQASVAMGPQKITYVSCN
CCCCEEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECC
PATMARDIKRYQELGYKLAKVQPVDLFPQTHHVECVVLLIKE
HHHHHHHHHHHHHHCCEEHEEECCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12122206; 12799345 [H]