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The map label for this gene is trmE [H]

Identifier: 19746040

GI number: 19746040

Start: 868879

End: 870255

Strand: Reverse

Name: trmE [H]

Synonym: spyM18_1045

Alternate gene names: 19746040

Gene position: 870255-868879 (Counterclockwise)

Preceding gene: 19746041

Following gene: 19746017

Centisome position: 45.92

GC content: 42.41

Gene sequence:

>1377_bases
ATGAGTATAACTAAAGAATTTGATACAATTACCGCCATTTCAACCCCTCTTGGTGAAGGAGCTATTGGCATTGTTCGTCT
ATCCGGGACAGATGCGCTTGCCATTGCACAATCTGTTTTTAAGGGAAAGAACTTAGAGCAAGTCGCCTCACACACCATTA
ACTATGGCCATATCATTGATCCTAAGACAGGAACTATTATCGATGAAGTTATGGTGTCAGTCATGCTAGCCCCCAAAACC
TTTACCCGTGAAAATGTGGTTGAAATCAATACGCACGGTGGTATTGCTGTGACAAATGAAATCTTGCAACTATTGATTCG
CCAAGGCGCTAGAATGGCAGAGCCTGGCGAATTCACCAAACGTGCCTTCTTAAATGGTCGTGTTGATTTGACTCAAGCTG
AGGCTGTCATGGACATTATTCGTGCCAAAACAGACAAGGCTATGACGATTGCAGTCAAGCAACTGGATGGCTCCCTTTCC
CAGCTTATCAATGACACCCGTCAGGAAATTCTCAACACCTTAGCTCAAGTCGAAGTCAATATTGACTACCCTGAATACGA
TGATGTGGAAGAAATGACCACTGCTCTGCTACGTGAGAAAACACAAGAATTCCAAAGCCTTTTGGAAAATCTTCTTCGAA
CAGCTAAACGCGGTAAAATTCTGCGTGAGGGCTTATCAACAGCTATTATTGGTCGTCCAAACGTCGGTAAGTCAAGCTTA
CTTAATAATTTGTTGCGAGAAGACAAGGCTATTGTGACTGATATTGCAGGAACCACCCGAGATGTCATTGAAGAATACGT
TAATATCAAGGGAGTTCCTCTAAAACTTGTCGATACGGCCGGTATTCGTGAGACAGATGATCTGGTCGAACAAATCGGAG
TTGAACGGTCTAAAAAAGCTCTTCAAGAAGCTGACCTTGTTCTTCTTGTGCTGAATGCTTCTGAAAAACTGACCGACCAA
GACCGGGCTTTGTTAAATCTCAGTCAGGACAGTAACCGTATTATATTGTTGAATAAAACGGATTTGGAACAAAAGATTGA
ATTAGAGCAATTACCAGATGACTACATTCCCATTTCCGTTTTAACCAACCAGAACATTAATCTAATCGAAGATCGTATCA
ACCAGCTTTTCTTTGACAACGCTGGTTTGGTAGAGCAAGATGCCACTTACCTGTCAAATGCCCGCCACATTTCTTTGATT
GAAAAGGCAGTCCAGAGTTTAGAAGCTGTCAATGACGGTTTGGCGCTTGGCATGCCAGTTGATTTACTACAGGTTGATTT
AACCAGAACTTGGGAAATTCTCGGAGAAATCACAGGGGATGCTGCTCCTGATGAACTCATTACCCAACTCTTTAGTCAAT
TCTGTCTTGGTAAATAA

Upstream 100 bases:

>100_bases
TATTTTTTTAGTATTAGCTAAACTCAAAATGGTGCTAGTCCTATTTTCTATACAAAATAATACCTTGCTTTCTACTAGGA
CTTGATTTAAAATAGAGGGC

Downstream 100 bases:

>100_bases
CAACAGTCTCTAATCATCACAAAAGAGCTGGAACAGTGTTCCAGCTCTTTTTGCTGTCATAAGCATCTTATTTAATTAAT
TCGTAGATTGCTTCAGCATA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKNLEQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKT
FTRENVVEINTHGGIAVTNEILQLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRGKILREGLSTAIIGRPNVGKSSL
LNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKLVDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQ
DRALLNLSQDSNRIILLNKTDLEQKIELEQLPDDYIPISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLSNARHISLI
EKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK

Sequences:

>Translated_458_residues
MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKNLEQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKT
FTRENVVEINTHGGIAVTNEILQLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRGKILREGLSTAIIGRPNVGKSSL
LNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKLVDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQ
DRALLNLSQDSNRIILLNKTDLEQKIELEQLPDDYIPISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLSNARHISLI
EKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK
>Mature_457_residues
SITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKNLEQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKTF
TRENVVEINTHGGIAVTNEILQLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLSQ
LINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRGKILREGLSTAIIGRPNVGKSSLL
NNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKLVDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQD
RALLNLSQDSNRIILLNKTDLEQKIELEQLPDDYIPISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLSNARHISLIE
KAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=467, Percent_Identity=32.9764453961456, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=31.4629258517034, Blast_Score=220, Evalue=3e-57,
Organism=Homo sapiens, GI193082993, Length=457, Percent_Identity=32.1663019693654, Blast_Score=215, Evalue=8e-56,
Organism=Escherichia coli, GI2367268, Length=462, Percent_Identity=33.5497835497836, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI87082120, Length=126, Percent_Identity=42.0634920634921, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=30.5194805194805, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6323665, Length=500, Percent_Identity=30.8, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI45550813, Length=474, Percent_Identity=29.535864978903, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR003661
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50538; Mature: 50406

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKNLEQVASHTINYGHIID
CCCCCHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEC
PKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEILQLLIRQGARMAEPGEFTK
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHH
RAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLSQLINDTRQEILNTLAQVEVN
HHHHCCCCCHHHHHHHHHHHHHCCCCHHEEHHHHHCHHHHHHHHHHHHHHHHHHHHEEEE
IDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRGKILREGLSTAIIGRPNVGKSSL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHH
LNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKLVDTAGIRETDDLVEQIGVERSKKA
HHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHH
LQEADLVLLVLNASEKLTDQDRALLNLSQDSNRIILLNKTDLEQKIELEQLPDDYIPISV
HHHCCEEEEEECCCCCCCCHHHHHEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCCEEE
LTNQNINLIEDRINQLFFDNAGLVEQDATYLSNARHISLIEKAVQSLEAVNDGLALGMPV
EECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCH
DLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKNLEQVASHTINYGHIID
CCCCHHHHHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHHHCCCCCEEEC
PKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEILQLLIRQGARMAEPGEFTK
CCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHHH
RAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLSQLINDTRQEILNTLAQVEVN
HHHHCCCCCHHHHHHHHHHHHHCCCCHHEEHHHHHCHHHHHHHHHHHHHHHHHHHHEEEE
IDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRGKILREGLSTAIIGRPNVGKSSL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHH
LNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKLVDTAGIRETDDLVEQIGVERSKKA
HHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCHHHHHH
LQEADLVLLVLNASEKLTDQDRALLNLSQDSNRIILLNKTDLEQKIELEQLPDDYIPISV
HHHCCEEEEEECCCCCCCCHHHHHEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCCEEE
LTNQNINLIEDRINQLFFDNAGLVEQDATYLSNARHISLIEKAVQSLEAVNDGLALGMPV
EECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCH
DLLQVDLTRTWEILGEITGDAAPDELITQLFSQFCLGK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]