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The map label for this gene is dnaB2 [H]

Identifier: 197294836

GI number: 197294836

Start: 848017

End: 849186

Strand: Reverse

Name: dnaB2 [H]

Synonym: PAa_0822

Alternate gene names: 197294836

Gene position: 849186-848017 (Counterclockwise)

Preceding gene: 197294837

Following gene: 197294835

Centisome position: 96.5

GC content: 24.96

Gene sequence:

>1170_bases
ATGATAAGCAATCGTTTTACCTTATTTAAAATTAAAAATCAAATTTCTTTATCATTTGAAGACCATCAAACTCTTAGTTT
ACTTTATCAACCATTAATAGGTGCTCAAGCGTTAAGTATTTATTATATTTTATTGTCTTTAAAAGCTAATTTCGAATATC
AACATCAATTTTTATTTGATATCGCTTTTATTTCAGAAGATGTTTTTTTAGAACAAAAAGAAAAATTAGAAGCACTTAAT
TTATTGGCTACTTATCAAAAAGAAAATCCTCGGGAAACAATTTATTTGGTTTTTCCTCCTTATAAAAGTAATGCTTTTTT
GCAAGACCCACTTTTAGGTGATTTTTTATTAAGTGAGATGGGTGAAAAAAATTATTTTCATTTGGAAAGCCATTTTTTAT
TAAAGCCCCTCAATTTAGAAGGTTTTCAAGATATTAGCAAAAAATTTGATGATATATATCAATTTGAAAAAATCAATTTT
AACCAAAATATTCAACAACACAAAACCATGAACCCTTTTGATAAGAGTTTTTTGCAAAGCAACTTTGATTATGAAGCATT
TTTATCATCCTTGCCAGAACGTTTTAAAAAAACTTTTTTAGTAGAGTCGCAAACAATTGACTTTATTAATAAATTAAGTT
TAGTTTATAATTTAACTCCTCAAAAAATAGCAGAAATTTATCAAAATACTTTTCCTAATCCTAATGATATAGATTTATCC
AAGTTACGTTTAAATGTCAAAAAAAATTATCATCAAAATAATAAAGAACAAATCATTATTTCTTCTAAAAAAACAGGCTA
TAACGAAGAAGAAATGATTACTTTTTTAAAAAAACCTAATGCAACTCAAAGAATTATTAAAGGTTATTGTCAAAAAAGTA
ATCAAACCATTGCAAGTGATACCGTTTTTCAGTTATTAGAAAGAAATGATTTAGAAATAGGTTTAGTTAACGCATTATTA
ATTTATATTTTAAGGTATCATAATGGTATTTTGCCTTCAGTTGTTTATTTAGAAAAAACTTTAAAATCGTGGTCCCAAAA
AGGTATTTTAGATGCAGAAACAGCTTATGATTTTTTAATGGAAGAAAAAACCGAAAAGCAAGTCAACTTTAAAAAACCAA
AAGATAAACCGAAATGGATAGACGAAATTAAAAAAAATTGGCAATCTTAA

Upstream 100 bases:

>100_bases
ACTGTTTGCATGACAAATATTATTAAAATCAAGGTTGGTGGCAGGGGAACTTATCTTTGTCCTTCATGTCAGCCAATTAA
ATAAAAAAAGAGGTTATAAA

Downstream 100 bases:

>100_bases
TTTGTTTATGAATTTTTAAAGATTTCCAATGTTTTTATCAGCTATCAAAAATTTAACTGTTGCAAAAATGAGAGATATTT
GCGCTTTTAAGAAAATCTTT

Product: Replication initiation/membrane attachment protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MISNRFTLFKIKNQISLSFEDHQTLSLLYQPLIGAQALSIYYILLSLKANFEYQHQFLFDIAFISEDVFLEQKEKLEALN
LLATYQKENPRETIYLVFPPYKSNAFLQDPLLGDFLLSEMGEKNYFHLESHFLLKPLNLEGFQDISKKFDDIYQFEKINF
NQNIQQHKTMNPFDKSFLQSNFDYEAFLSSLPERFKKTFLVESQTIDFINKLSLVYNLTPQKIAEIYQNTFPNPNDIDLS
KLRLNVKKNYHQNNKEQIIISSKKTGYNEEEMITFLKKPNATQRIIKGYCQKSNQTIASDTVFQLLERNDLEIGLVNALL
IYILRYHNGILPSVVYLEKTLKSWSQKGILDAETAYDFLMEEKTEKQVNFKKPKDKPKWIDEIKKNWQS

Sequences:

>Translated_389_residues
MISNRFTLFKIKNQISLSFEDHQTLSLLYQPLIGAQALSIYYILLSLKANFEYQHQFLFDIAFISEDVFLEQKEKLEALN
LLATYQKENPRETIYLVFPPYKSNAFLQDPLLGDFLLSEMGEKNYFHLESHFLLKPLNLEGFQDISKKFDDIYQFEKINF
NQNIQQHKTMNPFDKSFLQSNFDYEAFLSSLPERFKKTFLVESQTIDFINKLSLVYNLTPQKIAEIYQNTFPNPNDIDLS
KLRLNVKKNYHQNNKEQIIISSKKTGYNEEEMITFLKKPNATQRIIKGYCQKSNQTIASDTVFQLLERNDLEIGLVNALL
IYILRYHNGILPSVVYLEKTLKSWSQKGILDAETAYDFLMEEKTEKQVNFKKPKDKPKWIDEIKKNWQS
>Mature_389_residues
MISNRFTLFKIKNQISLSFEDHQTLSLLYQPLIGAQALSIYYILLSLKANFEYQHQFLFDIAFISEDVFLEQKEKLEALN
LLATYQKENPRETIYLVFPPYKSNAFLQDPLLGDFLLSEMGEKNYFHLESHFLLKPLNLEGFQDISKKFDDIYQFEKINF
NQNIQQHKTMNPFDKSFLQSNFDYEAFLSSLPERFKKTFLVESQTIDFINKLSLVYNLTPQKIAEIYQNTFPNPNDIDLS
KLRLNVKKNYHQNNKEQIIISSKKTGYNEEEMITFLKKPNATQRIIKGYCQKSNQTIASDTVFQLLERNDLEIGLVNALL
IYILRYHNGILPSVVYLEKTLKSWSQKGILDAETAYDFLMEEKTEKQVNFKKPKDKPKWIDEIKKNWQS

Specific function: Probable component of primosome involved in the initiation of DNA replication. It is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110 [H]

COG id: COG3611

COG function: function code L; Replication initiation/membrane attachment protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006343 [H]

Pfam domain/function: PF07261 DnaB_2 [H]

EC number: NA

Molecular weight: Translated: 45917; Mature: 45917

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISNRFTLFKIKNQISLSFEDHQTLSLLYQPLIGAQALSIYYILLSLKANFEYQHQFLFD
CCCCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IAFISEDVFLEQKEKLEALNLLATYQKENPRETIYLVFPPYKSNAFLQDPLLGDFLLSEM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHC
GEKNYFHLESHFLLKPLNLEGFQDISKKFDDIYQFEKINFNQNIQQHKTMNPFDKSFLQS
CCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHC
NFDYEAFLSSLPERFKKTFLVESQTIDFINKLSLVYNLTPQKIAEIYQNTFPNPNDIDLS
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHH
KLRLNVKKNYHQNNKEQIIISSKKTGYNEEEMITFLKKPNATQRIIKGYCQKSNQTIASD
HHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHH
TVFQLLERNDLEIGLVNALLIYILRYHNGILPSVVYLEKTLKSWSQKGILDAETAYDFLM
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EEKTEKQVNFKKPKDKPKWIDEIKKNWQS
HHHHHHCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MISNRFTLFKIKNQISLSFEDHQTLSLLYQPLIGAQALSIYYILLSLKANFEYQHQFLFD
CCCCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IAFISEDVFLEQKEKLEALNLLATYQKENPRETIYLVFPPYKSNAFLQDPLLGDFLLSEM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHC
GEKNYFHLESHFLLKPLNLEGFQDISKKFDDIYQFEKINFNQNIQQHKTMNPFDKSFLQS
CCCCEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHC
NFDYEAFLSSLPERFKKTFLVESQTIDFINKLSLVYNLTPQKIAEIYQNTFPNPNDIDLS
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHH
KLRLNVKKNYHQNNKEQIIISSKKTGYNEEEMITFLKKPNATQRIIKGYCQKSNQTIASD
HHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHH
TVFQLLERNDLEIGLVNALLIYILRYHNGILPSVVYLEKTLKSWSQKGILDAETAYDFLM
HHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EEKTEKQVNFKKPKDKPKWIDEIKKNWQS
HHHHHHCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3027697; 3027671; 8969504; 9387221; 9384377 [H]