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The map label for this gene is tig [H]

Identifier: 197294734

GI number: 197294734

Start: 715233

End: 716519

Strand: Direct

Name: tig [H]

Synonym: PAa_0700

Alternate gene names: 197294734

Gene position: 715233-716519 (Clockwise)

Preceding gene: 197294733

Following gene: 197294735

Centisome position: 81.28

GC content: 26.65

Gene sequence:

>1287_bases
ATGGAAATTAAAAAAATTAATGATGAAAAGATCCAGTGTGGTTTTAAGGTTTTAACTGAAGAATTAGAACACCATCTTAC
TTTGGCTTATGAACAAATAAAACATAAAGTAGAAGTTAAAGGTTTTAGAAAAGGGCATGTACCAAGAAAAATTTTTGAAA
CTCGTTTTGGTAAAGAAGAATTATATAATAACGCTTTAGAAAAGATTGTTCAAAACAAATACAAAGAAGCTTTGGCCAAA
AAAACTTTTGAAAATATGGGAATGCCTAAAATAGTAGGACTAGAAGAAAAAAATTTCAAAGAAAAAAAAGACTTTCATTT
TAATTTGGAATTTAATCTTAAACCTAAAGTCGTTTTAGGTTCATATTTAGGAATCAAATTAAGTAAAGGTGATTTAGAAA
TCACAAAAGAAGAAATTAATACTCAAATTGATTCTTTATTAGACAGTAAGAAAAAATTAGTTCCTAAAAACAGTAACGTT
TTAAAAAACAACGACACAGCTATTTTTGATTTTGAAGGTTTTATTGATGACAAACCTTTTGAAGGCGGTAGTGCTAAAAA
TTTTAGTTTAGAAATCGGTTCTAAAAAATTTGTTCCTGGTTTTGAAGAACAAATGATAGGAATGAAAAAAGAAGAAACAA
AAAATATAACAATTACTTTTCCTAATGATTATCCTAAATCAGATTTAGCTAATAAAAAAGCTATTTTTAAAGTAACTTTG
CATGACCTCAAAACCAAAGTTAAACCTGAGTTAACAAATGAATTAGTTGTCTCTTTGCAATATCCTGAAATCAAAACTGT
AGAAGAGCTTCAAACCAAAGTTACAGAAGAACTTACAAAACAAAAAGAACAACAAAATAAAAGAAGAGTAGAAAAAGAAG
TTTTAGATAAAATCAAGCAAAATACACAACTTACAATTCCTTTAACTTTAATCGAGGAAGAAAAAAAAGACATTAAAAAT
AAATTTCAAGCTCAATTACAAAAACAAAAATTAACCTTAGAACAATATCAATCATATTTAGGAATTACTGAAGAAAAAAT
GGAACAAAATTGGCATGAAGAAGCAAAAAACAACTTAGAATACCAACTTATTATGGAACAAGTCGCTTTGCAAGAAAAAA
TAACCGTCAACCCAGAAAAAATAGAACAATGTTACAAAGATTTAAGTCAAATGCATCAAATGTCACTTGAACAAGTTAAG
CAGAATTTAGATCTTGAAGTTTTAAAGCAAAATTTATTGTTAGATGAAGCTTGGGAATTAGTAATGAAAAATGTTGTTTT
CGCTTAA

Upstream 100 bases:

>100_bases
ATTTAAAAAAAGAAATGTTTAGCAAACTAATCTTTTTTATTTTTAAATGATGCAATATCATCAAATACGAAGTTATAAAC
TTAGAAAAAGGTATTGAAAA

Downstream 100 bases:

>100_bases
TTAAAAAGTGAAAAGGAGTTCAAAATGAAAACAAAAATAATGAAAAAAACTAAAACTGTTGTAGATTTAGAACAAAACAC
ACAAACGCAATTACCAGCTT

Product: Trigger factor (TF)

Products: peptidylproline (omega=0)

Alternate protein names: TF [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MEIKKINDEKIQCGFKVLTEELEHHLTLAYEQIKHKVEVKGFRKGHVPRKIFETRFGKEELYNNALEKIVQNKYKEALAK
KTFENMGMPKIVGLEEKNFKEKKDFHFNLEFNLKPKVVLGSYLGIKLSKGDLEITKEEINTQIDSLLDSKKKLVPKNSNV
LKNNDTAIFDFEGFIDDKPFEGGSAKNFSLEIGSKKFVPGFEEQMIGMKKEETKNITITFPNDYPKSDLANKKAIFKVTL
HDLKTKVKPELTNELVVSLQYPEIKTVEELQTKVTEELTKQKEQQNKRRVEKEVLDKIKQNTQLTIPLTLIEEEKKDIKN
KFQAQLQKQKLTLEQYQSYLGITEEKMEQNWHEEAKNNLEYQLIMEQVALQEKITVNPEKIEQCYKDLSQMHQMSLEQVK
QNLDLEVLKQNLLLDEAWELVMKNVVFA

Sequences:

>Translated_428_residues
MEIKKINDEKIQCGFKVLTEELEHHLTLAYEQIKHKVEVKGFRKGHVPRKIFETRFGKEELYNNALEKIVQNKYKEALAK
KTFENMGMPKIVGLEEKNFKEKKDFHFNLEFNLKPKVVLGSYLGIKLSKGDLEITKEEINTQIDSLLDSKKKLVPKNSNV
LKNNDTAIFDFEGFIDDKPFEGGSAKNFSLEIGSKKFVPGFEEQMIGMKKEETKNITITFPNDYPKSDLANKKAIFKVTL
HDLKTKVKPELTNELVVSLQYPEIKTVEELQTKVTEELTKQKEQQNKRRVEKEVLDKIKQNTQLTIPLTLIEEEKKDIKN
KFQAQLQKQKLTLEQYQSYLGITEEKMEQNWHEEAKNNLEYQLIMEQVALQEKITVNPEKIEQCYKDLSQMHQMSLEQVK
QNLDLEVLKQNLLLDEAWELVMKNVVFA
>Mature_428_residues
MEIKKINDEKIQCGFKVLTEELEHHLTLAYEQIKHKVEVKGFRKGHVPRKIFETRFGKEELYNNALEKIVQNKYKEALAK
KTFENMGMPKIVGLEEKNFKEKKDFHFNLEFNLKPKVVLGSYLGIKLSKGDLEITKEEINTQIDSLLDSKKKLVPKNSNV
LKNNDTAIFDFEGFIDDKPFEGGSAKNFSLEIGSKKFVPGFEEQMIGMKKEETKNITITFPNDYPKSDLANKKAIFKVTL
HDLKTKVKPELTNELVVSLQYPEIKTVEELQTKVTEELTKQKEQQNKRRVEKEVLDKIKQNTQLTIPLTLIEEEKKDIKN
KFQAQLQKQKLTLEQYQSYLGITEEKMEQNWHEEAKNNLEYQLIMEQVALQEKITVNPEKIEQCYKDLSQMHQMSLEQVK
QNLDLEVLKQNLLLDEAWELVMKNVVFA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=365, Percent_Identity=25.2054794520548, Blast_Score=132, Evalue=3e-32,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: 5.2.1.8

Molecular weight: Translated: 50074; Mature: 50074

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKKINDEKIQCGFKVLTEELEHHLTLAYEQIKHKVEVKGFRKGHVPRKIFETRFGKEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHH
LYNNALEKIVQNKYKEALAKKTFENMGMPKIVGLEEKNFKEKKDFHFNLEFNLKPKVVLG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEEECCCCCEEEE
SYLGIKLSKGDLEITKEEINTQIDSLLDSKKKLVPKNSNVLKNNDTAIFDFEGFIDDKPF
HHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCCCCCC
EGGSAKNFSLEIGSKKFVPGFEEQMIGMKKEETKNITITFPNDYPKSDLANKKAIFKVTL
CCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCHHCCCCCEEEEEH
HDLKTKVKPELTNELVVSLQYPEIKTVEELQTKVTEELTKQKEQQNKRRVEKEVLDKIKQ
HHHHHHCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NTQLTIPLTLIEEEKKDIKNKFQAQLQKQKLTLEQYQSYLGITEEKMEQNWHEEAKNNLE
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCH
YQLIMEQVALQEKITVNPEKIEQCYKDLSQMHQMSLEQVKQNLDLEVLKQNLLLDEAWEL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VMKNVVFA
HHHHHCCC
>Mature Secondary Structure
MEIKKINDEKIQCGFKVLTEELEHHLTLAYEQIKHKVEVKGFRKGHVPRKIFETRFGKEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHH
LYNNALEKIVQNKYKEALAKKTFENMGMPKIVGLEEKNFKEKKDFHFNLEFNLKPKVVLG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCEEEEEEECCCCCEEEE
SYLGIKLSKGDLEITKEEINTQIDSLLDSKKKLVPKNSNVLKNNDTAIFDFEGFIDDKPF
HHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCCCCCC
EGGSAKNFSLEIGSKKFVPGFEEQMIGMKKEETKNITITFPNDYPKSDLANKKAIFKVTL
CCCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCCCHHCCCCCEEEEEH
HDLKTKVKPELTNELVVSLQYPEIKTVEELQTKVTEELTKQKEQQNKRRVEKEVLDKIKQ
HHHHHHCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NTQLTIPLTLIEEEKKDIKNKFQAQLQKQKLTLEQYQSYLGITEEKMEQNWHEEAKNNLE
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCH
YQLIMEQVALQEKITVNPEKIEQCYKDLSQMHQMSLEQVKQNLDLEVLKQNLLLDEAWEL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VMKNVVFA
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (omega=180)

Specific reaction: peptidylproline (omega=180) = peptidylproline (omega=0)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA