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The map label for this gene is thiI [H]

Identifier: 197294645

GI number: 197294645

Start: 594560

End: 595873

Strand: Direct

Name: thiI [H]

Synonym: PAa_0601

Alternate gene names: 197294645

Gene position: 594560-595873 (Clockwise)

Preceding gene: 197294597

Following gene: 197294646

Centisome position: 67.57

GC content: 28.46

Gene sequence:

>1314_bases
ATGGAGCAAAAAAGAATTTTAATCCGTTTTGGTGAACTTTTTTTAAAAGGAAAAAATAAAAAATATTTTATTCAAAAATT
AAAAGAGCTTTTGCAAAACAAGTTAAAAAATTTAGCCAATATTAATTTACAAGTTCAACATGATTACGCTTATCTAGATT
ATTGTTGTCATTTAGAAAAGGAAGTTTTGAAAAGATTATCTTATGTTTCCGGAATTGCTTCTTTTGTGATTGTTCAACAA
GCCGAAAAAAATATTGATGATATCGTTTCCAAAGCTTCTTTGATGTTAAAAGAAAAAATCAAAAAACCAGTTTTATTTAA
AATTGAAACAATAAGGAAAGATAAAAGTTTTTTTTTAACTAGTCTAGAATTAACTCAAAAAGTTGCCCCTTTAATTCTAC
AATCCTTACAAGGTTTATTGAAAGTAGATGTTAAAAAACCTGAAGTAATTTTAAACATTGAAGTCAAAAAAGAAAAAATT
TATCTTTATTGTCAAAACGACCAAAAGAAAGCTTTGGGAGGGTTCCCCAATTTTTCTTCTGGAAAAGGGCTTGTTTTTTT
AAGTGGAGGAATTGACTCGCCAGTAGCAGCTTATTTAGCGATGAAAAAAGGGATTGCGATTGAGTTGTTACATTTTGAAG
CAAGTCCGCTAACGCCTTTAGAATCAGTCCAAAAAGTAATTGATTTAGCAAAGATTTTAAGTCGTTATTCTGCTAACGGT
CAGGTTAAATTACATTTAATTCCTTTTAGGACTTTACAAGAAACTATTCATCAAAAAGTTTCAGATGCTTATATTATTAA
CGTTATGCGCCGAATGATGTATCGTTTGGGTGATCAAATAGCAAAAACTAACCACCATTTATGTTTAATTAATGGAGAAT
CGGTAGGACAAGTGGCTAGTCAAACTTTGGAAAGCATGCAAACGACTTCTTGCATTACTAATATCAATATTTTAAGACCT
TTAGCAATGATGGACAAAAACCAAATTATTGAGATTGCTAAAAACATTGAAACCTTTGATATTTCAATTCGACCTTTTCA
AGATTGCTGCACTGTTTATCTTCCTTCAAGACCTGTTACCAAGCCAAAAACAAAAATATCCGAAAAAGAAGAAAATTATT
TTGATTATCAACATTTATTAAAGACTACTTTAGAACAAACAGTTACTTTACAAATTGAAGAAAATTTAGATTTTAATCTT
TTGGACTTTGGTTTTTATAATGTTAAAGAAGCGATTGATAATTTTTGTCAAAAACATCCCAAACAAAAAATAATTTTGAA
AAACACAAAACTTATGCAAAATACAACAAAATAA

Upstream 100 bases:

>100_bases
TATTTTTTTTGGTTTTTTCAAAAATCTCCAAAAATCATCTTCAAAAATAAAATTATTATTTATTTAATCTAAACTAAAAT
ACTGATTTGAGGTAATATTT

Downstream 100 bases:

>100_bases
AAAAATCATGAACAAAAACTGCATAAAAAAAGAATTTCCAAAACGATTTAATAAAAAATGCTATTAAAAAAAGAAAAAAA
TTATGGATGAAAAAATTTTA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MEQKRILIRFGELFLKGKNKKYFIQKLKELLQNKLKNLANINLQVQHDYAYLDYCCHLEKEVLKRLSYVSGIASFVIVQQ
AEKNIDDIVSKASLMLKEKIKKPVLFKIETIRKDKSFFLTSLELTQKVAPLILQSLQGLLKVDVKKPEVILNIEVKKEKI
YLYCQNDQKKALGGFPNFSSGKGLVFLSGGIDSPVAAYLAMKKGIAIELLHFEASPLTPLESVQKVIDLAKILSRYSANG
QVKLHLIPFRTLQETIHQKVSDAYIINVMRRMMYRLGDQIAKTNHHLCLINGESVGQVASQTLESMQTTSCITNINILRP
LAMMDKNQIIEIAKNIETFDISIRPFQDCCTVYLPSRPVTKPKTKISEKEENYFDYQHLLKTTLEQTVTLQIEENLDFNL
LDFGFYNVKEAIDNFCQKHPKQKIILKNTKLMQNTTK

Sequences:

>Translated_437_residues
MEQKRILIRFGELFLKGKNKKYFIQKLKELLQNKLKNLANINLQVQHDYAYLDYCCHLEKEVLKRLSYVSGIASFVIVQQ
AEKNIDDIVSKASLMLKEKIKKPVLFKIETIRKDKSFFLTSLELTQKVAPLILQSLQGLLKVDVKKPEVILNIEVKKEKI
YLYCQNDQKKALGGFPNFSSGKGLVFLSGGIDSPVAAYLAMKKGIAIELLHFEASPLTPLESVQKVIDLAKILSRYSANG
QVKLHLIPFRTLQETIHQKVSDAYIINVMRRMMYRLGDQIAKTNHHLCLINGESVGQVASQTLESMQTTSCITNINILRP
LAMMDKNQIIEIAKNIETFDISIRPFQDCCTVYLPSRPVTKPKTKISEKEENYFDYQHLLKTTLEQTVTLQIEENLDFNL
LDFGFYNVKEAIDNFCQKHPKQKIILKNTKLMQNTTK
>Mature_437_residues
MEQKRILIRFGELFLKGKNKKYFIQKLKELLQNKLKNLANINLQVQHDYAYLDYCCHLEKEVLKRLSYVSGIASFVIVQQ
AEKNIDDIVSKASLMLKEKIKKPVLFKIETIRKDKSFFLTSLELTQKVAPLILQSLQGLLKVDVKKPEVILNIEVKKEKI
YLYCQNDQKKALGGFPNFSSGKGLVFLSGGIDSPVAAYLAMKKGIAIELLHFEASPLTPLESVQKVIDLAKILSRYSANG
QVKLHLIPFRTLQETIHQKVSDAYIINVMRRMMYRLGDQIAKTNHHLCLINGESVGQVASQTLESMQTTSCITNINILRP
LAMMDKNQIIEIAKNIETFDISIRPFQDCCTVYLPSRPVTKPKTKISEKEENYFDYQHLLKTTLEQTVTLQIEENLDFNL
LDFGFYNVKEAIDNFCQKHPKQKIILKNTKLMQNTTK

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=330, Percent_Identity=27.2727272727273, Blast_Score=122, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 50166; Mature: 50166

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQKRILIRFGELFLKGKNKKYFIQKLKELLQNKLKNLANINLQVQHDYAYLDYCCHLEK
CCCCHHHHEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHH
EVLKRLSYVSGIASFVIVQQAEKNIDDIVSKASLMLKEKIKKPVLFKIETIRKDKSFFLT
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCHHHHHH
SLELTQKVAPLILQSLQGLLKVDVKKPEVILNIEVKKEKIYLYCQNDQKKALGGFPNFSS
HHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEEEEEEEEEEECCCHHHCCCCCCCCC
GKGLVFLSGGIDSPVAAYLAMKKGIAIELLHFEASPLTPLESVQKVIDLAKILSRYSANG
CCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
QVKLHLIPFRTLQETIHQKVSDAYIINVMRRMMYRLGDQIAKTNHHLCLINGESVGQVAS
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHH
QTLESMQTTSCITNINILRPLAMMDKNQIIEIAKNIETFDISIRPFQDCCTVYLPSRPVT
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEEEEEECCHHHHHEEEECCCCCC
KPKTKISEKEENYFDYQHLLKTTLEQTVTLQIEENLDFNLLDFGFYNVKEAIDNFCQKHP
CCCHHCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHCC
KQKIILKNTKLMQNTTK
CCEEEEECCHHHHCCCC
>Mature Secondary Structure
MEQKRILIRFGELFLKGKNKKYFIQKLKELLQNKLKNLANINLQVQHDYAYLDYCCHLEK
CCCCHHHHEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHH
EVLKRLSYVSGIASFVIVQQAEKNIDDIVSKASLMLKEKIKKPVLFKIETIRKDKSFFLT
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCHHHHHH
SLELTQKVAPLILQSLQGLLKVDVKKPEVILNIEVKKEKIYLYCQNDQKKALGGFPNFSS
HHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEEEEEEEEEEEEEECCCHHHCCCCCCCCC
GKGLVFLSGGIDSPVAAYLAMKKGIAIELLHFEASPLTPLESVQKVIDLAKILSRYSANG
CCEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
QVKLHLIPFRTLQETIHQKVSDAYIINVMRRMMYRLGDQIAKTNHHLCLINGESVGQVAS
EEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHH
QTLESMQTTSCITNINILRPLAMMDKNQIIEIAKNIETFDISIRPFQDCCTVYLPSRPVT
HHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCEEEEEEECCHHHHHEEEECCCCCC
KPKTKISEKEENYFDYQHLLKTTLEQTVTLQIEENLDFNLLDFGFYNVKEAIDNFCQKHP
CCCHHCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHCC
KQKIILKNTKLMQNTTK
CCEEEEECCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA